期刊
VACCINE
卷 31, 期 47, 页码 5524-5530出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2013.09.024
关键词
Infectious bronchitis virus; VLPs; Vaccine
资金
- Chinese National High-tech R&D Program (863 Program) [2011AA10A209]
- cooperation project in industry, education and research of Guangdong province
- Ministry of Education of People's Republic of China [2010B090301019]
Infectious bronchitis virus (IBV) as an avian coronavirus is still posing a persistent and imminent threat to the poultry industry worldwide. Here we report that transfection of Sf9 cells with a single recombinant baculovirus encoding M and S proteins resulted in the assembly of IBV VLPs; this is the first report that S protein plus M protein alone were able to be assembled into VLPs for coronaviruses. We further showed that the generated IBV VLPs could induce humoral immune responses in a level comparable to that of inactivated IBV vaccine, and more importantly the IBV VLPs could elicit significantly higher cellular immune responses than the inactivated IBV vaccine. In summary, the assembly of IBV VLPs with M and S proteins provided a simple strategy for generating VLPs for coronaviruses, and the generated IBV VLPs laid a feasible foundation for the development of an effective vaccine against infection of IBV in the future. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据