期刊
VACCINE
卷 30, 期 48, 页码 6845-6850出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.09.011
关键词
Foot-and-Mouth Disease Virus; Cross-protection; Avidity; Subtypes
资金
- FMD-DISCONVAC [226556]
- European Commission within the Seventh Frame Programme
- Agencia Nacional de Promocion Cientifica y Tecnologica, Argentina [PID-87 PAE 37206]
Serological assessment of the heterologous response among Foot-and-Mouth Disease Virus (FMDV) strains is mainly performed by virus neutralization test (VNT), liquid phase blocking ELISA and complement fixation assay. In this study two high-throughput ELISA techniques, avidity and IgG subtype ELISA, were developed and used to further characterize heterologous antibody responses in cattle during vaccination and challenge. Both assays were applied to a set of previously characterized sera from animals immunized with an inactivated A24 Cruzeiro/Brazil/55 (A24 Cruzeiro) strain monovalent FMDV vaccine and challenged with the heterologous A/Argentina/2001 (A/Arg/01) strain. Single dilution avidity ELISA assessment showed that animals that were protected against A/Arg/01 challenge had higher avidity antibodies to this heterologous strain than non-protected cattle. Animals with low or even undetectable anti-A/Arg/01 serum-neutralizing titers that passed the heterologous challenge presented higher IgG1/IgG2 ratio than non-protected animals. In this study, the three assessments (VNT and both ELISAs) discriminated between protected and not protected animals against a heterologous challenge. The combination of these techniques may be applied to complement current indirect serological vaccine-matching assessments. The measurement of these qualitative parameters may provide additional information to understand the mechanisms underlying FMD heterologous responses and the induction of cross-protection in cattle. (C) 2012 Elsevier Ltd. All rights reserved.
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