Review
Chemistry, Medicinal
Mohamed Ahmed Attia, Ebtessam Ahmed Essa, Toka Tarek Elebyary, Ahmed Mostafa Faheem, Amal Ali Elkordy
Summary: Vaccination is the most effective means of preventing infectious diseases, and advancements in biotechnology and nanoparticles have enabled successful vaccine development. Liposomes, as a versatile carrier for vaccines, have shown great potential in delivering antigens and eliciting desired immune responses during the ongoing pandemic of COVID-19.
Review
Pharmacology & Pharmacy
Jeroen Heuts, Wim Jiskoot, Ferry Ossendorp, Koen van der Maaden
Summary: Cationic nanoparticles have shown to be effective in cancer immunotherapy by delivering tumor-associated antigens to dendritic cells and inducing strong antigen-specific cellular immune responses. Lipid- and polymer-based cationic nanoparticles are utilized for different types of cancer vaccines, and their potential in T-cell induction is discussed for therapeutic cancer vaccination.
Review
Pharmacology & Pharmacy
Dylan A. Hendy, Eva A. Amouzougan, Isabella C. Young, Eric M. Bachelder, Kristy M. Ainslie
Summary: Influenza is a global disease affecting millions of people, and vaccination is the best prevention method. However, due to viral mutations, seasonal influenza vaccines often have low efficacy and require yearly vaccination. To increase the antigenicity of universal influenza vaccines, nano/microparticles have been utilized as carriers, showing promising results in animal models.
Article
Veterinary Sciences
Camilo Jaramillo-Morales, Kaitlyn James, Samantha Barnum, Wendy Vaala, Duane E. Chappell, Chrissie Schneider, Bryant Craig, Fairfield Bain, D. Craig Barnett, Earl Gaughan, Nicola Pusterla
Summary: This study aimed to describe selected prevalence factors of horses with acute onset of fever and respiratory signs testing positive for S. equi, as well as the impact of vaccination on S. equi detection.
VETERINARY SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Beata Biri-Kovacs, Zoltan Banoczi, Anitha Tummalapally, Ildiko Szabo
Summary: Skin cancer is the most common type of cancer, with melanoma being a leading cause of skin cancer deaths. Recent advancements in understanding melanoma tumorigenesis and its interaction with the immune system have led to the development of new therapeutic strategies. Peptide-based vaccines, which target tumor antigens and activate immune responses, have shown promise in treating advanced melanoma. However, the immunogenicity of peptide antigens can be enhanced through synthetic modifications, adjuvants, and delivery systems. This review summarizes the various chemical approaches for developing effective peptide-based vaccines for immunotherapy in advanced melanoma.
Review
Medicine, Research & Experimental
Despo Chatzikleanthous, Derek T. O'Hagan, Roberto Adamo
Summary: Despite the progress in vaccine adjuvants, there are still unmet needs for vaccines against challenging pathogens like HIV, tuberculosis, and cancer. Liposomes have shown to be effective as adjuvant/delivery systems and have potential for further use in vaccine development, as seen in recent COVID-19 vaccines. This review outlines different approaches for lipid-based vaccine adjuvant/delivery systems and how they can be combined to develop multicomponent vaccines.
MOLECULAR PHARMACEUTICS
(2021)
Article
Engineering, Multidisciplinary
Ana Maria Carmona-Ribeiro
Summary: This article explores the role of nanovaccines in preventing or treating cancer, infectious diseases, and diseases caused by parasites. The main properties of novel adjuvants are nanosize, cationic character, and biocompatibility, even at low doses.
Review
Immunology
Arianna Raponi, James M. Brewer, Paul Garside, Donatello Laera
Summary: Purified vaccine antigens offer safety advantages compared to live vaccines, but adjuvants like aluminum salts are needed to induce the required immune response. By reducing aluminum particle size from micro to nanometers, a more effective adjuvant can be obtained, with the ability to sterile filter the vaccine product.
SEMINARS IN IMMUNOLOGY
(2021)
Review
Chemistry, Physical
Aihua Qu, Maozhong Sun, Liguang Xu, Liqiang Liu, Lingling Guo, Panpan Chen, Qing Wang, Zhiyong Du, Zhimeng Wu, Chuanlai Xu, Hua Kuang
Summary: Chiral nanomaterials play a crucial role in the field of vaccines by promoting antigen presentation and activating the immune system for tumor immunotherapy. However, there are still challenges in this research, such as finding better synthesis methods and addressing the issue of immune system regulation.
Review
Biochemistry & Molecular Biology
Eugenia D. Namiot, Aleksandr Sokolov, Vladimir N. Chubarev, Vadim V. Tarasov, Helgi B. Schioth
Summary: Nanoparticles, particularly lipid-based ones, have played a crucial role in the development and manufacturing of the Pfizer and Moderna COVID-19 vaccines. They offer advantages such as reduced toxicity and increased bioavailability, and can be modified for specific tissues or cells. The number of clinical trials using nanoparticles for drug delivery is growing rapidly due to advancements in nanotechnology, biotechnology, and medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Anna Wasniewska-Wlodarczyk, Renata Pepas, Renata Janowicz, Wieslaw Konopka
Summary: This report presents an extremely rare case of retropharyngeal abscess in a 12-year-old girl caused by an infection of Streptococcus equi subsp. equi.
Review
Immunology
Thai Thanh Hoang Thi, Estelle J. A. Suys, Jung Seok Lee, Dai Hai Nguyen, Ki Dong Park, Nghia P. Truong
Summary: This review highlights the importance of lipid-based nanoparticles in the development of COVID-19 vaccines and other nanomedicines, focusing on innovations that have obtained regulatory approval or are in clinical trials. It also discusses the physicochemical properties required for specific applications, emphasizes the differences in requirements for delivering different cargos, and introduces current challenges that need further development.
Review
Immunology
Javier Castrodeza-Sanz, Ivan Sanz-Munoz, Jose M. Eiros
Summary: In recent decades, improvements in traditional vaccines have led to the development of more processed vaccines with better tolerability profiles but lower immunogenicity. Adjuvants provide a good solution to enhance the immunogenicity of these vaccines with improved tolerability and fewer side effects. The approval of protein-based vaccines in 2022 and 2023 further expanded the options. Adjuvanted vaccines can induce potent immune responses in populations with weak or insufficient immune systems, such as the elderly, and could play a crucial role in completing global COVID-19 vaccination efforts in the present and future.
Review
Immunology
Sivakumar S. Moni, Siddig Ibrahim Abdelwahab, Aamena Jabeen, Mohamed Eltaib Elmobark, Duaa Aqaili, Gassem Ghoal, Bassem Oraibi, Abdulla Mohammed Farasani, Ahmed Ali Jerah, Mahdi Mohammed A. Alnajai, Abdul Majeed Hamad Mohammad Alowayni
Summary: This review provides a thorough analysis of the advances in adjuvant research and explores the evolution and effects of various adjuvants. Alum, as the pioneer, laid the foundation for further research and innovation. The transition from traditional alum-based adjuvants to nano formulations reflects the dynamism and potential of vaccine research. Innovations in adjuvant research, particularly the development of nano formulations, hold great promise for improving vaccine efficacy and safety.
Article
Immunology
Giuditta Guerrini, Antonio Vivi, Sabrina Gioria, Jessica Ponti, Davide Magri, Arnd Hoeveler, Donata Medaglini, Luigi Calzolai
Summary: Physicochemical properties of adjuvant-protein antigen complexes impact vaccine efficacy and safety, and a multi-staged characterization strategy has been developed for accurate assessment of nanoaluminum-ovalbumin complex, which can be adapted for various adjuvant-protein antigen combinations to control quality and improve immunological outcomes of vaccines.
Review
Food Science & Technology
Andreia Nunes, Joana Marto, Li'dia Goncalves, Ana Margarida Martins, Carmo Fraga, Helena Margarida Ribeiro
Summary: The by-products of the olive oil industry, containing phenolic compounds harmful to the environment, have potential for recovery and reuse for their health-promoting properties. These compounds extracted from the by-products have numerous health benefits for skin health and therapeutics, contributing to the sustainable development of the olive oil industry.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Beatriz Silva, Lidia M. Goncalves, Berta Sao Braz, Esmeralda Delgado
Summary: This study investigates the delivery of EPO beta to the eye through subconjunctival administration of chitosan-hyaluronic acid-EPO beta nanoparticles. The results show that these nanoparticles can efficiently deliver EPO beta into the retina without adverse effects. This nanoformulation could be a promising tool for treating retinopathies.
Article
Geography
A. Almeida, V. Rato, Z. F. Dabaja
Summary: Studies in Scandinavian and Anglo-Saxon countries have shown a decline in outdoor activities and contact with nature during childhood. This study, conducted in Lisbon, Portugal, involved 562 urban children and 303 parents, and found a decrease in outdoor activities among children during weekdays, weekends, and summer holidays. It was also noted that schools are no longer promoting outdoor activities and children are spending their leisure time indoors.
CHILDRENS GEOGRAPHIES
(2023)
Article
Biochemistry & Molecular Biology
David Pires, Manoj Mandal, Jacinta Pinho, Maria Joao Catalao, Antonio Jose Almeida, Jose Miguel Azevedo-Pereira, Maria Manuela Gaspar, Elsa Anes
Summary: Mycobacterium tuberculosis establishes chronic colonization in lung macrophages through controlled replication, leading to latent infection. Manipulation of endolysosomal enzymes, cathepsins, by the pathogen is crucial for intracellular survival. Liposomal delivery of saquinavir, a protease inhibitor, enhances its internalization in macrophages and exhibits significant intracellular killing effects on drug-susceptible and drug-resistant Mycobacterium tuberculosis strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ana F. Bettencourt, Joana Costa, Isabel A. C. Ribeiro, Lidia Goncalves, Maria Teresa Arias-Moliz, Juliana R. Dias, Margarida Franco, Nuno M. Alves, Jaime Portugal, Cristina B. Neves
Summary: The high recurrence rate of common denture stomatitis after antifungal treatment is still a concern. Impregnating denture materials with antimicrobials for local delivery is a strategy to improve treatment efficacy. In this study, three hard autopolymerizing reline acrylic resins loaded with different percentages of chlorhexidine (CHX) were developed and characterized. Probase Cold loaded with 5 wt% CHX showed the most promising results in terms of antimicrobial activity and lack of serious detrimental properties.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Beatriz Silva, Lidia M. Goncalves, Berta Sao Braz, Esmeralda Delgado
Summary: This study explored the possibility of delivering EPO beta to the posterior segment of the eye using a chitosan-hyaluronic acid nanoparticulate system via topical instillation. The results showed that topical administration of CS/HA nanoparticles did not cause adverse ocular effects and had no significant impact on microhematocrits or electroretinography. Immunofluorescence staining demonstrated the presence of EPO beta in the retina for up to 21 days after administration. In conclusion, topical administration of CS/HA nanoparticles could efficiently deliver EPO beta to the retina and has the potential for retinal neuroprotection.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Radoslaw Starosta, Telma C. Santos, Andreia F. Dinis F. de Sousa, Maria Soledade Santos, M. Luisa Corvo, Ana Isabel Tomaz, Rodrigo F. M. de Almeida
Summary: This study investigates the possible role of the cell membrane in the mechanism of action of two ruthenium complexes against human tumor cells. The results show that these complexes increase the permeability of lipid vesicles and alter the biophysical properties of the membranes. Moreover, the effects of the complexes depend on changes in membrane lipid composition.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Medicinal
Beatriz Silva, Lidia M. Goncalves, Berta Sao Braz, Esmeralda Delgado
Summary: The study investigated the effects of CS/HA-EPO beta nanoparticles on a rat glaucoma model. The rats were divided into treatment group and control group, and ocular examinations were performed. The treated group showed faster recovery of retinal electrical activity and EPO beta was detected in their retinas. The study suggests that topical ocular administration of CS/HA-EPO beta nanoparticles can deliver EPO beta to the retina and promote retinal recovery.
Article
Pharmacology & Pharmacy
Paula Leandro, Paulo R. Lino, Raquel Lopes, Joao Leandro, Mariana P. Amaro, Paulo Sousa, Joao B. Vicente, Antonio J. Almeida
Summary: The structural maintenance of therapeutic proteins during formulation and/or storage is crucial. Protein freeze-drying is a commonly used technique for preserving protein structure and function. In this study, differential scanning fluorimetry (DSF) and isothermal denaturation fluorimetry (ITDF) were used to screen for the most effective stabilizer for the model protein human phenylalanine hydroxylase (hPAH) among different freeze-drying additives. ITDF was found to be the most suitable screening method based on correlation studies with recovered enzyme amount and activity. The selected compounds prevented protein aggregation and preserved hPAH structural and functional properties throughout long-term storage.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Instruments & Instrumentation
Ivan Lamela-Gomez, Lidia M. Goncalves, Antonio J. Almeida, Asteria Luzardo-alvarez
Summary: Microencapsulation of infliximab (INF), a monoclonal antibody, using ultrasonic atomization (UA) and conventional emulsion/evaporation method (Em/Ev) was investigated for improved stability and intra-articular administration. UA achieved higher encapsulation efficiency (69.7-80.25%) compared to Em/Ev (17.3-23.0%). Particle size ranged from 26.6 to 49.9 μm for UA and 1.5-2.1 μm for Em/Ev. Both methods demonstrated sustained INF release and preserved biological activity. Microencapsulated INF exhibited higher efficacy in tumor necrosis factor-alpha (TNF alpha) neutralization and anti-inflammatory activity.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Giulia Nerli, Lidia M. D. Goncalves, Marzia Cirri, Antonio J. Almeida, Francesca Maestrelli, Natascia Mennini, Paola A. Mura
Summary: There is a need for pediatric drug formulations to avoid safety and quality risks associated with the use of adult dosage forms. In this study, chitosan nanoparticles (CSNPs) and nanostructured lipid carriers (NLCs) were developed as potential nanocarriers for oral pediatric solutions of cefixime. The CSNPs showed better stability and drug release profiles compared to NLCs, making them a promising option for pediatric drug delivery.
Article
Polymer Science
Valentina Sallustio, Giovanna Farruggia, Massimiliano Pio di Cagno, Martina M. Tzanova, Joana Marto, Helena Ribeiro, Lidia Maria Goncalves, Manuela Mandrone, Ilaria Chiocchio, Teresa Cerchiara, Angela Abruzzo, Federica Bigucci, Barbara Luppi
Summary: Rising environmental awareness has led to an increased demand for sustainable cosmetics made from natural bioactive compounds. This study aimed to develop an eco-friendly anti-aging gel containing Rosa canina L. extract. The extract was encapsulated in ethosomal vesicles with varying concentrations of ethanol, and the formulations were tested for size, polydispersity, zeta potential, entrapment efficiency, release, skin penetration/permeation, and cell viability. The ethosomes were then incorporated into hyaluronic gels and evaluated for rheological properties. The results showed that the ethosomes containing 30% ethanol effectively encapsulated the rosehip extract, and the resulting gel formulation exhibited optimal stability and spreadability for skin application. This innovative and green skincare product has potential in the anti-aging market.
Review
Pharmacology & Pharmacy
M. Zegre, E. Poljanska, L. A. Caetano, L. Goncalves, A. Bettencourt
Summary: The treatment of bone infections involves high doses of antibiotics, either systemically or locally. Recent research has focused on developing drug delivery systems that can target the infected bone specifically, reducing the amount of drugs needed and their potential toxicity. Biodegradable polymeric platforms, such as micro- and nanoparticles, scaffolds, and hydrogels, are being studied as alternative options to the current gold standard treatment, poly(methyl methacrylate) (PMMA) cement.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)