期刊
VACCINE
卷 29, 期 46, 页码 8405-8411出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.08.035
关键词
Foot-and-mouth disease; VP1 G-H loop; Marker vaccine
资金
- BBSRC
Contrary to to the dogma that the VP1 G-H loop is essential for FMD vaccine efficacy, it has been previously shown that foot-and-mouth disease 146s antigen containing heterologous VP1 G-H loops confers complete protection in pigs and cattle. Moreover, serological evaluation of cattle vaccinated with an antigen lacking a large proportion of the VP1 G-H loop indicated that these animals should be protected against infection with FMD. Absence of this loop provides opportunity for the development of an FMD negative marker vaccine, allowing infection to be detected by antibodies against this missing region. Cattle vaccinated with this negative marker vaccine were fully protected following virus challenge 28 days post vaccination as determined by the absence of generalised lesions on their feet. Furthermore, use of our improved differentiation ELISA identified animals exposed to infection as early as 7 days post-challenge. We thus demonstrate, for the first time, the ability of this FMD negative marker vaccine to fully protect cattle from experimental challenge and rapidly distinguish animals that are subsequently exposed to infection. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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