期刊
VACCINE
卷 29, 期 38, 页码 6695-6703出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.06.092
关键词
Flagellin; Adjuvant; Transgenic plant; Oral immunization
资金
- La Fondation de l'Universite du Quebec a Montreal
- FARE (Fond de l'Accessibilite a la Reussite des Etudiants de l'Universite du Quebec A Montreal)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Institutes of Health Research (NSER/CIHR)
The aim of this study was to produce adjuvant with high biosafety, efficacy and low cost. Towards this goal, the plant Nicotiana benthamiana transient expression system was successfully used to express Salmonella typhimurium's flagellin (FljB). The yield of the expressed FljB was 280 mg per kg of fresh weight (FAN) leaves. The lyophilized plant powder containing plant expressing FljB was mixed with ovalbumin (OVA) and used for oral immunization of BALB/c mice. The ELISA analysis showed higher and accelerated OVA-specific serum antibody responses in mice given the mixture when compared to animals receiving OVA alone. Furthermore, FljB elicited a mixed Th1/Th2 response as shown by the presence of specific anti-OVA IgG1, IgG2a and IgG2b isotypes. OVA-specific IgAs were also detected in mice given the mixture. Cell-mediated immune response to OVA was induced by FljB as determined by a spleen lymphocyte specific proliferation test. No immune response was generated against FljB. In conclusion, our results showed for the first time the production of FljB in plants and the efficient use of the crude lyophilized extract as an adjuvant for oral immunization. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据