Article
Veterinary Sciences
Remil L. Galay, Carina R. Llaneta, Maria Karla Faye B. Monreal, Antero L. Armero, Arianne Bel D. Baluyut, Czarina Marie F. Regino, Kristina Andrea C. Sandalo, Billy P. Divina, Melbourne R. Talactac, Lennox P. Tapawan, Maarten Czar L. Mojares, Cherry R. Alvarez, Emmanuel R. Mago, Noemi D. Encarnacion, Masako Andoh, Tetsuya Tanaka
Summary: The study investigated the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines. It found a high prevalence of A. marginale in the region and provided the first molecular evidence of E. minasensis in the country.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Edgar Dantan-Gonzalez, Rosa Estela Quiroz-Castaneda, Hugo Aguilar-Diaz, Itzel Amaro-Estrada, Fernando Martinez-Ocampo, Sergio Rodriguez-Camarillo
Summary: This study analyzed the genetic diversity of seven Mexican strains of A. marginale for the first time and found their phylogeographic relationships with strains from North America, South America, and Asia.
Article
Infectious Diseases
Maria Evangelina Primo, Julio Bellezze, Nicolas Morel, Matilde Mazzucco Panizza, Beatriz Susana Valentini, Susana Marta Torioni, Carolina Soledad Thompson
Summary: A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the highly conserved msp5 gene of Anaplasma spp. was developed and evaluated in field samples. The assay showed high sensitivity and agreement with other specific nPCR methods for the detection of A. marginale and A. centrale.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Immunology
Yu-ming Peng, Jian-jun Tao, Su-fang Kuang, Ming Jiang, Xuan-xian Peng, Hui Li
Summary: This study identified potential polyvalent vaccine candidates against bacterial infections by constructing DNA vaccines from extracellular secretory proteins of Vibrio alginolyticus. Three of the vaccines showed cross-immune protective effects against various bacterial infections, indicating the possibility of using extracellular secretory proteome as an antigen pool for identifying cross-protective immunogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Muna Salem M. Solyman, Jessica Ujczo, Kelly A. Brayton, Dana K. Shaw, David A. Schneider, Susan M. Noh
Summary: This study discovers the importance of iron for the replication of Anaplasma marginale, a pathogen causing bovine anaplasmosis, in tick cells. It identifies the involvement of specific proteins in iron transport and their differential expression in response to iron levels in host cells. This foundational work could lead to interventions to prevent bovine and human anaplasmosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
V. A. Teixeira, A. M. Q. Lana, T. Bresolin, T. R. Tomich, G. M. Souza, J. Furlong, J. P. P. Rodrigues, S. G. Coelho, L. C. Goncalves, J. A. G. Silveira, L. D. Ferreira, E. J. Facury Filho, M. M. Campos, J. R. R. Dorea, L. G. R. Perira
Summary: Bovine anaplasmosis causes significant economic losses in dairy cattle production systems worldwide, and conventional detection methods are laborious and costly. This study explores the early identification of anaplasmosis using wearable sensors and recurrent neural networks. The findings suggest a great potential for wearable sensors in early detection of anaplasmosis diseases.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
R. S. Souza, M. F. S. Resende, L. C. A. Ferreira, R. S. Ferraz, M. V. V. Araujo, C. Bastos, J. A. G. Silveira, T. F. Moreira, R. M. Meneses, A. U. Carvalho, F. O. P. Leme, E. J. Facury Filho
Summary: The study evaluated the monitoring of tick fever in a Brazilian dairy farm and found that the current protocols overestimate the occurrence of the disease, leading to overtreatment. Proposing a new monitoring protocol based on rectal temperature and microscopic analysis could result in significant cost savings, rational use of medication, and reduced resistance to antimicrobials.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Hanene Belkahia, Meriem Ben Abdallah, Rihab Andolsi, Rachid Selmi, Sayed Zamiti, Myriam Kratou, Moez Mhadhbi, Mohamed Aziz Darghouth, Lilia Messadi, Mourad Ben Said
Summary: Molecular, phylogenetic, and immuno-informatics analyses of Tunisian isolates revealed a 2.4% prevalence of A. marginale DNA, with cattle from subhumid bioclimatic areas showing higher infection rates. The analysis of lipA phylogeographic marker indicated higher genetic diversity in Tunisian A. marginale isolates compared to worldwide isolates and strains. The conservation analysis of the OmpA protein vaccine candidate demonstrated conserved immunodominant epitopes, suggesting potential cross-protective capacity against multiple A. marginale strains.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Zaibullah Khan, Shehla Shehla, Abdulaziz Alouffi, Muhammad Kashif Obaid, Alam Zeb Khan, Mashal M. Almutairi, Muhammad Numan, Ome Aiman, Shumaila Alam, Shafi Ullah, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali
Summary: Ticks infesting livestock in different districts of Pakistan were molecularly characterized in this study, and nine species of hard ticks were identified, with Anaplasma marginale being detected. The findings are important for further understanding the diversity of tick species and associated pathogens in Pakistan.
Article
Veterinary Sciences
Pablo Parodi, Maria T. Armua-Fernandez, Marcos Schanzembach, Daiana Mir, Maria Jose Benitez-Galeano, Nelida Rodriguez-Osorio, Rodolfo Rivero, Jose M. Venzal
Summary: This study characterized the genetic diversity of Anaplasma marginale in Uruguay for the first time and investigated its relationship with Rhipicephalus microplus. Different genotypes and tandem repeat combinations of the MSP1a gene were identified. Furthermore, a portion of the strains lacked the ability to bind to tick cells, suggesting potential transmission by other vectors.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Parasitology
Joshua Kamani, Abdul Matin M. N. Mubo Qasim, Toyin A. Olubade, Moses Samuel Abasiama, Ali Gajibo, Peter Umar Balami, Mike Shands, Shimon Harrus
Summary: Bovine anaplasmosis caused by Anaplasma marginale is a widespread and significant endemic disease in developing countries. This study analyzed samples from Nigerian cattle and identified three different nucleotide sequence types of A. marginale, providing the first nationwide report on its diversity and genetic characterization.
Article
Microbiology
Witchuta Junsiri, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Pornpiroon Nooroong, Sathaporn Jittapalapong, Runglawan Chawengkirttikul, Panat Anuracpreeda
Summary: The study focused on determining the genetic diversity and antigenicity of Anaplasma marginale in cattle in Thailand based on the msp1a and msp1b genes. Results revealed diverse tandem repeat sequences in the MSP1a gene and the presence of novel conserved tandem sequences named TH1 and TH2. Phylogenetic analysis showed genetic diversity in the msp1a and msp1b genes among the isolates, with various haplotypes identified. Additionally, B-cell and T-cell epitopes were investigated, providing insights into the molecular phylogenetics, genetic diversity, and antigenicity of the A. marginale Thailand strain.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Dhruba Das, Kalyan Sarma, Chethan Gollahalli Eregowda, Parimal Roychoudhury, Justus Babykutty Rajesh, Parthasarathi Behera, Hridayesh Prasad, Hniang Lalrinkima, Fatema Aktar, Nikitasha Bora, Champak Deka, Neeraj Thakur, Thingujam Chaa Tolenkhomba
Summary: This study aimed to investigate the distribution of Anaplasma species infection in cattle from the Aizawl region of Mizoram, India, and analyze the associated risk factors. The study also identified haemato-biochemical alterations, oxidant/antioxidant status, and serum trace mineral levels in cattle with naturally occurring Anaplasma marginale infection.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Shumaila Alam, Mehran Khan, Abdulaziz Alouffi, Mashal M. Almutairi, Shafi Ullah, Muhammad Numan, Nabila Islam, Zaibullah Khan, Ome Aiman, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali
Summary: This study investigated the tick prevalence, host range, genetic diversity, and molecular survey of Anaplasma spp. in different tick species in the southern regions of Khyber Pakhtunkhwa (KP), Pakistan. The results revealed variations in infection rates and genetic characteristics among different tick species, and provided the first molecular level information on Haemaphysalis cornupunctata.
Article
Veterinary Sciences
Hassan Y. A. H. Mahmoud, Alsagher O. Ali, Tetsuya Tanaka
Summary: In this study, novel molecular techniques were used to detect and characterize A. marginale in cattle, buffalo, and camel populations in southern Egypt. The prevalence of A. marginale infection was found to be 61% in cattle, 12% in buffaloes, and 6.66% in camels. Phylogenetic analysis provided new data on A. marginale infections in camels. Screening for A. marginale is recommended even in the absence of clinical signs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Editorial Material
Veterinary Sciences
Francisco O. Conrado, Michael J. Dark, Sarah S. K. Beatty
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2019)
Editorial Material
Veterinary Sciences
Kellie M. Whipple, Elizabeth A. Kieran, Michael J. Dark, Sarah S. Beatty
VETERINARY CLINICAL PATHOLOGY
(2020)
Article
Veterinary Sciences
Shannon Darby, Erin Porter, Sarah S. K. Beatty, Michael J. Dark, Andrew Smith, Ramiro E. Toribio, Diego E. Gomez
JOURNAL OF EQUINE VETERINARY SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Firas M. Abed, Melissa A. Brown, Omar A. Al-Mahmood, Michael J. Dark
Article
Veterinary Sciences
C. J. Fisher, A. T. Lejeune, M. J. Dark, O. M. Hernandez, K. Shiomitsu
Summary: Ponatinib has shown cytotoxic effects on canine mast cell tumor cell lines, inducing apoptosis. The study confirmed the expression of three tyrosine kinase receptors and highlighted ponatinib as a potential therapeutic agent for canine mast cell tumors. Further research is needed to explore the prognostic value of these receptors in canine MCTs.
VETERINARY JOURNAL
(2021)
Article
Immunology
Matthew J. Cascio, Elizabeth M. Whitley, Bikash Sahay, Galaxia Cortes-Hinojosa, Lung-Ji Chang, Jonathan Cowart, Marc Salute, Elias Sayour, Michael Dark, Zachary Sandoval, Duane A. Mitchell, Rowan J. Milner
Summary: The expression of PD-L1, HVEM, and B7H3 in osteosarcoma was found to be associated with metastatic lesions and correlated with each other. The presence of these ligands on osteosarcoma cells positively correlated with peritumoral T cell infiltration. Osteosarcoma showed significant resistance to effector T cells but multiple agents targeting checkpoints may have immune modulating benefits in this context.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Veterinary Sciences
J. Quesada, L. Londono, G. J. Buckley, M. J. Dark, J. C. Colee, L. L. Farina
Summary: The study found that closed chest cardiopulmonary resuscitation can lead to iatrogenic complications in dogs, with pulmonary hemorrhage being the most common complication. Severe bleeding may impact the recurrence of cardiac arrest following successful resuscitation.
JOURNAL OF SMALL ANIMAL PRACTICE
(2021)
Editorial Material
Pathology
Donald J. Meuten, Frances M. Moore, Taryn A. Donovan, Christof A. Bertram, Robert Klopfleisch, Robert A. Foster, Rebecca C. Smedley, Michael J. Dark, Milan Milovancev, Paul Stromberg, Bruce H. Williams, Marc Aubreville, Giancarlo Avallone, Pompei Bolfa, John Cullen, Michelle M. Dennis, Michael Goldschmidt, Richard Luong, Andrew D. Miller, Margaret A. Miller, John S. Munday, Paola Roccabianca, Elisa N. Salas, F. Yvonne Schulman, Renee Laufer-Amorim, Midori G. Asakawa, Linden Craig, Nick Dervisis, D. Glen Esplin, Jeanne W. George, Marlene Hauck, Yumiko Kagawa, Matti Kiupel, Keith Linder, Kristina Meichner, Laura Marconato, Michelle L. Oblak, Renato L. Santos, R. Mark Simpson, Harold Tvedten, Derick Whitley
Summary: Standardization of tumor assessment is crucial for validating grading systems, ensuring reproducibility in oncologic studies, and increasing confidence in study results. This article provides standard guidelines for tumor assessment parameters to address the lack of methodological standardization in veterinary medicine. By updating and revising these guidelines regularly and providing them as living documents on a website, collaboration between pathologists and institutions can be facilitated to advance veterinary oncologic pathology and patient care.
VETERINARY PATHOLOGY
(2021)
Article
Veterinary Sciences
Kallie J. Hobbs, Erin Porter, Courtney Wait, Michael Dark, Robert J. MacKay
Summary: This study retrospectively describes the MRI features and size of normal equine pituitary glands and explores the correlation between pituitary size, brain size, and patient weight. The findings can serve as a reference for MRI evaluation of equine pituitary glands.
VETERINARY RADIOLOGY & ULTRASOUND
(2022)
Article
Veterinary Sciences
Julia L. Engelien, Amandine T. Lejeune, Michael J. Dark, Rowan J. Milner, Keijiro Shiomitsu
Summary: This study investigates the use of RNA in situ hybridisation (ISH) technology for the diagnosis of canine histiocytic sarcoma (HS). The results demonstrate that CD204 and IBA-1 have high specificity and sensitivity in identifying HS, making RNA-ISH a valuable diagnostic technique.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Multidisciplinary Sciences
Taranpreet Rai, Ambra Morisi, Barbara Bacci, Nicholas J. Bacon, Michael J. Dark, Tawfik Aboellail, Spencer Angus Thomas, Miroslaw Bober, Roberto La Ragione, Kevin Wells
Summary: This article describes a novel method for automated necrosis detection in whole slide images using deep learning AI. By testing on a canine soft tissue sarcoma dataset, the optimized DenseNet-161 model demonstrated state-of-the-art performance, representing a significant step forward in reproducible and reliable necrosis assessment for improving the precision of tumor grading.
SCIENTIFIC REPORTS
(2022)
Article
Computer Science, Artificial Intelligence
Marc Aubreville, Nikolas Stathonikos, Christof A. Bertram, Robert Klopfleisch, Natalie ter Hoeve, Francesco Ciompi, Frauke Wilm, Christian Marzahl, Taryn A. Donovan, Andreas Maier, Jack Breen, Nishant Ravikumar, Youjin Chung, Jinah Park, Ramin Nateghi, Fattaneh Pourakpour, Rutger H. J. Fick, Saima Ben Hadj, Mostafa Jahanifar, Adam Shephard, Jakob Dexl, Thomas Wittenberg, Satoshi Kondo, Maxime W. Lafarge, Viktor H. Koelzer, Jingtang Liang, Yubo Wang, Xi Long, Jingxin Liu, Salar Razavi, April Khademi, Sen Yang, Xiyue Wang, Ramona Erber, Andrea Klang, Karoline Lipnik, Pompei Bolfa, Michael J. Dark, Gabriel Wasinger, Mitko Veta, Katharina Breininger
Summary: The density of mitotic figures (MF) in tumor tissue is an important marker for tumor grading, but its recognition by pathologists is biased and limited. Deep learning methods can support the recognition, but their performance deteriorates in different clinical environments due to variability caused by using different whole slide scanners. The MICCAI MIDOG 2021 challenge aimed to develop scanner-agnostic MF detection algorithms and the winning algorithm outperformed six experts on the same task.
MEDICAL IMAGE ANALYSIS
(2023)
Letter
Pathology
F. Yvonne Schulman, Christof A. Bertram, Donald J. Meuten, Frances M. Moore, Giancarlo Avallone, Alexander Bartel, Pompei Bolfa, Melinda Camus, James K. Chambers, Michael J. Dark, Nick Dervisis, Melanie J. Dobromylskyj, Taryn A. Donovan, Robert A. Foster, Paula M. Krimer, Andrew Miller, Milan Milovancev, Paola Roccabianca, Laura E. Selmic, Geoff A. Wood
VETERINARY PATHOLOGY
(2023)
Article
Veterinary Sciences
Ambra Morisi, Taran Rai, Nicholas J. Bacon, Spencer A. Thomas, Miroslaw Bober, Kevin Wells, Michael J. Dark, Tawfik Aboellail, Barbara Bacci, Roberto M. La Ragione
Summary: Canine soft-tissue sarcomas are tumors that arise from the skin and subcutaneous connective tissue. The use of artificial intelligence algorithms and machine learning can help automatically detect tumor necrosis and improve the efficiency and accuracy of tumor grading.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Valentine D. Verpaalen, J. Brad Case, Michael J. Dark, S. Joel Cardenas-Goicoechea, Matt D. Winter, Sarah E. Boston, Fernando Garcia-Pereira, Alice S. Rhoton-Vlasak, Beau B. Toskich
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2020)