Article
Fisheries
Xifeng Hu, Xiangdong Wu, Meijia Xue, Yiting Chen, Beiyi Zhou, Tong Wan, Hongnan You, Huansheng Wu
Summary: The study found that chTAX1 inhibits IFN expression by promoting degradation of chicken MAVS, and after IBDV infection, the distribution of chTAX1 changes and co-localizes with VP1 and VP3. Overexpression of chTAX1 promotes IBDV replication.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Xifeng Hu, Xiangdong Wu, Zhen Ding, Zheng Chen, Huansheng Wu
Summary: It has been found that chhnRNPU inhibits IFN-I3 production via interacting with MDA5 and MAVS, and facilitates IBDV replication via associating with genomic dsRNA of IBDV.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Bing Yang, Xiaochun Wu, Jingqi Zeng, Jinjing Song, Tianhao Qi, Yanjun Yang, Dingkun Liu, Yulin Mo, Miao He, Liang Feng, Xiaobin Jia
Summary: This study investigated the potential of natural polysaccharides as carriers to improve the biopharmaceutical properties of active components. It was found that natural polysaccharides can self-assemble into aggregates and form complexes with the active components, thereby enhancing their stability, solubility, permeability, and absorption in the gastrointestinal tract.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Medicine, Research & Experimental
Yu Du, Haitong Wan, Ping Huang, Jiehong Yang, Yu He
Summary: Astragalus polysaccharides (APs), as important active ingredients of Astragali Radix, have therapeutic potential for multiple diseases. However, their low bioavailability limits their clinical application. Nanocrystals and structural modification of APs have been shown to improve bioavailability, potentially expanding their application.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Microbiology
Elisabet Diaz-Beneitez, Liliana Lilibeth Cubas-Gaona, Oscar Candelas-Rivera, Ana Benito-Zafra, Maria Teresa Sanchez-Aparicio, Lisa Miorin, Jose F. Rodriguez, Adolfo Garcia-Sastre, Dolores Rodriguez
Summary: Infectious Bursal Disease Virus (IBDV) is an immunosuppressive disease that affects domestic chickens. This study found that TRIM25 plays an important role in the pathogenesis of IBDV by enhancing the activation of IFN-inducing pathway mediated by MDA5.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Wenlong Zhang, Ning Song, Yuan Gao, Xufeng Xie, Kun Liu, Yongguo Cao, Ningyi Jin
Summary: Leptospirosis, a neglected infectious disease, can cause serious health problems including kidney injury and bleeding disorders. This study found that astragalus polysaccharides (APS), a natural compound, can protect hamsters from acute leptospirosis by enhancing glycolysis.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Yanan Li, Junping Zheng, Yao Wang, Huabing Yang, Lu Cao, Shuiyong Gan, Jun Ma, Hongtao Liu
Summary: This study investigates the role of gut microbiota in the immune-enhancing activity of Astragalus polysaccharides (APS) in mice. The results show that APS can ameliorate immunosuppression in mice and activate Toll-like receptor 4 (TLR4) and mitogen-activated protein kinase (MAPK) pathways. APS treatment also reverses gut microbiota dysbiosis in immunocompromised mice.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Bing Yang, Zhiwei Xiong, Meng Lin, Yanjun Yang, Yaping Chen, Jingqi Zeng, Xiaobin Jia, Liang Feng
Summary: This study found that polysaccharides, the main components of Astragalus, a traditional Chinese medicine, can regulate the gut microbiota associated with type 1 diabetes (T1D). It improves gut barrier function, gut microbiota composition, lowers blood glucose, and increases insulin levels. This effect may be related to the production of short-chain fatty acids and the modulation of inflammatory response.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Guangming Chen, Nan Jiang, Junping Zheng, Haiming Hu, Huabing Yang, Aizhen Lin, Baifei Hu, Hongtao Liu
Summary: In this study, two homogeneous polysaccharides (APS-A1 and APS-B1) were isolated from Astragalus membranaceus and their chemical structures were characterized. APS-A1 had a 1,4-alpha-D-Glcp backbone with a 1,4,6-alpha-D-Glcp branch every ten residues, while APS-B1 was a heteropolysaccharide composed of glucose, galactose, and arabinose. Bioactivity assays showed that both APS-A1 and APS-B1 exhibited potential anti-inflammatory activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Xifeng Hu, Xiangdong Wu, Zheng Chen, Huansheng Wu
Summary: This study investigated the effect of chicken PRMT1 (chPRMT1) on regulating IFN-beta production and IBDV replication by constructing chPRMT1 knock out DF-1 cells. It was found that chPRMT1 was widely expressed in various tissues and localized in the cytoplasm and nucleus of DF-1 cells. chPRMT1 inhibited IFN-beta activation at the step of chMAVS. Additionally, chPRMT1 knock out DF-1 cells were constructed using CRISPR-Cas9 technique, and it was demonstrated that PRMT1 negatively regulated IBDV replication via suppressing IFN-beta production.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yan Yu, Lili Li, Rui Sun, Zhiyong Xu, Qiuxia Wang, Changbo Ou, Yanhong Zhang, Pei Gao, Jinyou Ma
Summary: The study revealed the important roles of PTEN gene in the distribution and development of immune organs in poultry, and the downregulation of PTEN expression in chickens infected with IBDV might be a mechanism for the virus to evade host immunity. Strategies aimed at restoring PTEN expression could potentially be a therapy for preventing IBDV infection.
Article
Chemistry, Applied
Yang Cai, Zhenyuan Si, Ying Jiang, Meng Ye, Feng Wang, Xiaobing Yang, Juping Yu, Xiangdong Gao, Wei Liu
Summary: This study investigated the structure-activity relationship of Astragalus membranaceus polysaccharides (APS) using two carbohydrate-active enzymes. The degradation products were divided into different groups based on their molecular weights. The results showed that APS-A1 and APS-G3 had better immunomodulatory activity, while APS-G1 and APS-G2 had weaker activity. Molecular interaction experiments revealed that APS-A1 and APS-G3 could bind to toll-like receptor-4 (TLR-4), while APS-G1 and APS-G2 could not. The branched chains of galactose or arabinogalactooligosaccharide played a crucial role in the immunomodulatory activity of APS.
CARBOHYDRATE POLYMERS
(2023)
Review
Biochemistry & Molecular Biology
Tasnim H. Beacon, James R. Davie
Summary: The chicken genome is one-third the size of the human genome and shares 60% similarity in gene content. Chickens have a gene arrangement closer to humans than rodents. They are used as model organisms to study evolution, epigenome, and diseases. The chicken nucleated erythrocyte's role in oxygen transport to tissues, removal of carbon dioxide, and innate immune response makes it a valuable subject for epigenomic studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wei Yu, Yukai Yang, Qicun Zhou, Xiaolin Huang, Zhong Huang, Tao Li, Qiaer Wu, Chuanpeng Zhou, Zhenhua Ma, Heizhao Lin
Summary: This study demonstrates that the supplementation of Astragalus polysaccharides (APS) can have beneficial effects on the growth, health, and resistance to Vibrio harveyi of Asian seabass. APS supplementation at 0.10% to 0.20% significantly improved the growth performance, digestive enzyme activity, and intestinal health of Asian seabass. Furthermore, APS supplementation enhanced the antioxidant capacity and immune function of the fish. However, a higher concentration of APS (0.20%) had adverse effects on the growth and immune response of Asian seabass.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Virology
Aijing Liu, Qing Pan, Suyan Wang, Yu Zhang, Yue Li, Yongqiang Wang, Xiaole Qi, Li Gao, Changjun Liu, Yanping Zhang, Hongyu Cui, Kai Li, Xiaomei Wang, Yulong Gao
Summary: Infectious bursal disease virus (IBDV) causes severe immunosuppressive disease in chickens, leading to significant economic losses in the poultry industry. This study identified and evaluated the chicken transmembrane protein cluster of differentiation 44 (chCD44) as a cellular receptor for IBDV, facilitating virus binding and entry into host cells.
JOURNAL OF VIROLOGY
(2022)
