Review
Immunology
Robert C. Brunham
Summary: This article discusses the approaches and challenges in developing a Chlamydia trachomatis vaccine, and summarizes two key research directions and unresolved issues in human C. trachomatis immunology.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Delia F. Tifrea, Wei He, Sukumar Pal, Angela C. Evans, Sean F. Gilmore, Nicholas O. Fischer, Amy Rasley, Matthew A. Coleman, Luis M. de la Maza
Summary: Researchers developed a cell-free co-translational system using tNLP to express the major outer membrane protein of Chlamydia muridarum, MOMP. The study demonstrated the protective ability of MOMP-tNLP+CpG+FSL-1 against intranasal challenge of C. muridarum in mice, showing significant immune responses and protection effects. This study marks the first successful demonstration of a MOMP-based vaccine formulated in tNLP providing protection against C. muridarum.
Article
Microbiology
Sukumar Pal, Anatoli Slepenkin, Jiin Felgner, D. Huw Davies, Philip Felgner, Luis M. de la Maza
Summary: There is an urgent need to develop a vaccine for Chlamydia infections. Four adjuvant combinations were tested for their local reactogenicity and ability to elicit protection against C. muridarum in mice. IVAX-1 and IVAX-2 showed minimal local reactogenicity, while vaccines containing Montanide resulted in the formation of local granuloma. The Th1-biased immune responses were observed in all four adjuvant combinations.
Article
Biochemistry & Molecular Biology
Dung T. Huynh, Wouter S. P. Jong, Gregory M. Koningstein, Peter van Ulsen, Joen Luirink
Summary: Ct-MOMP, a promising subunit-based vaccine candidate, is difficult to express in its native structure in E. coli outer membrane. Co-expression of the Bam complex improved the expression and localization of recombinant Ct-MOMP, leading to correct folding and assembly into a beta-barrel conformation at the cell surface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Junfei Wei, Venkatesh L. Hegde, Ananta V. Yanamandra, Madison P. O'Hara, Brian Keegan, Kathryn M. Jones, Ulrich Strych, Maria Elena Bottazzi, Bin Zhan, K. Jagannadha Sastry, Peter J. Hotez
Summary: Trichuriasis is a neglected tropical disease affecting the poorest populations. Researchers are developing a vaccine composed of Tm-WAP49 protein to be delivered through intranasal immunization. Preliminary results in a mouse model showed high levels of antibody response and significant reduction in worm burden. The addition of adjuvants further improved the vaccine efficacy, inducing robust cellular responses and long-term T cell memory.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Amanda L. Collar, Alexandria C. Linville, Susan B. Core, Kathryn M. Frietze
Summary: This study used immunogenic bacteriophage VLP technology to design a vaccine against Chlamydia trachomatis (Ct). Two methods were used to identify potential immunogens that could elicit neutralizing antibodies to Ct, and the resulting vaccines provided protection from Chlamydia infection in a mouse model.
Article
Immunology
Bo Peng, Shufang Zhong, Yaoqin Hua, Qizheng Luo, Weilei Dong, Chuan Wang, Zhongyu Li, Chunfen Yang, Aihua Lei, Chunxue Lu
Summary: Immunization with Pgp3 provides protection against Chlamydia infection, and the protective effect is dependent on the native conformation of Pgp3.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Richard I. Walker, A. Louis Bourgeois
Summary: Oral immunization is an effective strategy against mucosal enteric pathogens. Inactivated whole bacterial cells can also induce protective immunity. Developing a safe and simple delivery format with controlled immunogenic amounts of complex antigens is essential. Enhancing the efficacy of whole cell vaccines can be achieved through genetic engineering and the use of mucosal adjuvants. The development of ETVAX, a vaccine candidate against enterotoxigenic Escherichia coli (ETEC), has shown promising results.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sweta Karan, Devapriya Choudhury, Aparna Dixit
Summary: Vibrio parahaemolyticus is a major pathogen responsible for vibriosis and zoonotic infections. Its extracellular curli fibrils, specifically the adhesin CsgA, have been identified as potential vaccine candidates due to their role in adhesion and virulence. Immunization with recombinant soluble CsgA resulted in a strong immune response in mice, indicating its potential as an effective vaccine against V. parahaemolyticus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Immunology
Romina Cecilia Russi, Diego del Balzo, Ivana Gabriela Reidel, Mariano Alonso Bivou, Noelia Flor, Agustin Lujan, Diego Sanchez, Maria Teresa Damiani, Carolina Veaute
Summary: This study evaluated a vaccine based on a fragment of the Polymorphic membrane protein D (FPmpD) of C. trachomatis as an immunogen using a DNA prime-protein boost strategy. The results showed that both free recombinant FPmpD and recombinant FPmpD formulated with adjuvants increased the titers of anti-rFPmpD antibodies in mice and induced a rapid and robust humoral immune response upon Ct challenge. However, the adjuvant-containing vaccine formulations were more effective in reducing Ct shedding and preventing immunopathology compared to the vaccine with free rFPmpD.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Andrea Springer, Christian von Holtum, Georg von Samson-Himmelstjerna, Christina Strube
Summary: The lungworm Dictyocaulus viviparus is an economically important bovine parasite. A recombinant subunit vaccine based on the major sperm protein (MSP) was tested and found to induce specific antibody production. However, the vaccine alone did not significantly reduce worm fecundity.
Article
Immunology
Lanhua Zhao, Mingyi Shu, Hongliang Chen, Keliang Shi, Zhongyu Li
Summary: In this study, graphene oxide (GO)-stabilized Pickering emulsion (GPE) was used as an adjuvant to enhance the immune response. The GPE was prepared with small droplet sizes and demonstrated controlled release of antigens, enhanced macrophage uptake, polarization, and recruitment. The use of GPE as an adjuvant promoted augmented cellular and humoral immune responses and ameliorated genital tract damage.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Lind Throne Degn, Ditte Bech, Gunna Christiansen, Svend Birkelund
Summary: Chlamydia trachomatis is the major cause of sexually transmitted diseases and understanding the role of antibodies in its infection process is important for vaccine development. By investigating the specificity and binding characteristics of two monoclonal antibodies against the major outer membrane protein (MOMP) of C. trachomatis, this study provides insights into the protective contribution of antibodies and their limitations in reducing infectivity.
MOLECULAR IMMUNOLOGY
(2023)
Review
Immunology
Luis M. de la Maza, Toni L. Darville, Sukumar Pal
Summary: The article discusses the progress in research on the Chlamydia trachomatis vaccine and expects more vaccine clinical trials to be conducted in the next five years.
EXPERT REVIEW OF VACCINES
(2021)
Review
Immunology
Sam M. Murray, Paul F. McKay
Summary: Chlamydia trachomatis is a causative agent of a prevalent sexually transmitted bacterial disease, with current therapy options lacking protective immunity. Successful vaccine development requires understanding Chlamydia biology, immune correlates of protection, and lessons from previous vaccine attempts. Research on chlamydial immunity, key immune interactions, and timelines of vaccine development provide insights for potential solutions and further exploration.
Article
Immunology
Sukumar Pal, Olga V. Tatarenkova, Luis M. de la Maza
Article
Immunology
Delia F. Tifrea, Michael L. Barta, Sukumar Pal, P. Scott Hefty, Luis M. de la Maza
MICROBES AND INFECTION
(2016)
Article
Immunology
Sukumar Pal, Alison Favaroni, Delia F. Tifrea, Philipp T. Hanisch, Soeren E. T. Luczak, Johannes H. Hegemann, Luis M. de la Maza
Article
Immunology
Sukumar Pal, Delia F. Tifrea, Frank Follmann, Peter Andersen, Luis M. de la Maza
Article
Immunology
Delia F. Tifrea, Sukumar Pal, Jean-Luc Popot, Melanie J. Cocco, Luis M. de la Maza
JOURNAL OF IMMUNOLOGY
(2014)
Article
Immunology
Chunmei Cheng, Pooja Jain, Sukumar Pal, Delia Tifrea, Guifeng Sun, Andy A. Teng, Xiaowu Liang, Philip L. Felgner, Luis M. de la Maza
MICROBES AND INFECTION
(2014)
Article
Immunology
Chunmei Cheng, Sukumar Pal, Delia Tifrea, Zhenyu Jia, Luis M. de la Maza
MICROBES AND INFECTION
(2014)
Article
Immunology
Delia F. Tifrea, Sukumar Pal, Christel Le Bon, Fabrice Giusti, Jean-Luc Popot, Melanie J. Cocco, Manuela Zoonens, Luis M. de la Maza
Article
Immunology
Sukumar Pal, Delia F. Tifrea, Luis M. de la Maza
INFECTION AND IMMUNITY
(2019)
Article
Immunology
Delia F. Tifrea, Sukumar Pal, Luis M. de la Maza
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Biotechnology & Applied Microbiology
Sukumar Pal, Salvador Fernando Ausar, Delia F. Tifrea, Chunmei Cheng, Scott Gallichan, Violette Sanchez, Luis M. de la Maza, Lucian Visan
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2020)
Article
Multidisciplinary Sciences
Mufadhal Al-Kuhlani, Graham Lambert, Sukumar Pal, Luis de la Maza, David M. Ojcius
Article
Immunology
Delia F. Tifrea, Sukumar Pal, Jeff Fairman, Paola Massari, Luis M. de la Maza
Article
Immunology
Delia F. Tifrea, Sukumar Pal, Christel le Bon, Melanie J. Cocco, Manuela Zoonens, Luis M. de la Maza
Article
Immunology
Sukumar Pal, Delia F. Tifrea, Guangming Zhong, Luis M. de la Maza
INFECTION AND IMMUNITY
(2018)