期刊
VACCINE
卷 28, 期 15, 页码 2788-2798出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.01.050
关键词
Human parainfluenza virus; Live attenuated vaccine candidate; Human ciliated airway epithelium
资金
- NIH [R01 HL77844]
- NIAID
- Molecular Biology of Viral Diseases Training [5-T32-AI007419]
Human parainfluenza viruses (HPIVs) are common causes of severe pediatric respiratory viral disease. We characterized wild-type HPIV2 infection in an in vitro model of human airway epithelium (HAE) and found that the virus replicates to high titer, sheds apically, targets ciliated cells, and induces minimal cytopathology. Replication of an experimental, live attenuated HPIV2 vaccine strain, containing both temperature sensitive (ts) and non-ts attenuating mutations, was restricted >30-fold compared to rHPIV2-WT in HAE at 32 degrees C and exhibited little productive replication at 37 degrees C. This restriction paralleled attenuation in the upper and lower respiratory tract of African green monkeys, supporting the HAE model as an appropriate and convenient system for characterizing HPIV2 vaccine candidates. (C) 2010 Elsevier Ltd. All rights reserved.
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