Article
Chemistry, Analytical
Jiang Qian, Matthew P. Donohue, Thomas Bowen, Ying Zhang
Summary: The potency of inactivated influenzaviral vaccines is currently assessed using the SRID method. However, the time delay in producing the necessary reagents for SRID can be problematic. The LTD-IDMS method, which does not require antisera or reference antigens, has shown comparable results to SRID. In this study, a streamlined improvement to the LTD-IDMS method is demonstrated, saving time and labor in the sample preparation process.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Jesse Bodle, Kirsten Vandenberg, Karen Laurie, Ian G. Barr, Ying Zhang, Steven Rockman
Summary: This study aimed to determine the applicability of enzyme linked immunoassay (ELISA) in quantifying and assessing the stability of different influenza vaccines. The ELISA, utilizing stable-indicating monoclonal antibodies (mAbs), accurately measures the HA antigen content and stability, with higher sensitivity and specificity towards different influenza B virus strains. Moreover, the ELISA can accurately quantify HA from different platforms and processing stages. Therefore, it is a suitable replacement for the traditional single radial immunodiffusion (SRID) assay.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Tor Kristian Andersen, Johanna Bodin, Fredrik Oftung, Bjarne Bogen, Siri Mjaaland, Gunnveig Grodeland
Summary: The 2009 swine flu pandemic exposed the limitations of egg-based vaccines in terms of global supply, prompting the urgent need for efficient new vaccine platforms; DNA vaccines have since been developed as a temperature stable, cost-effective option for pandemic responses, capable of inducing rapid protective immune responses without the need for adjuvants.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Kartik Narayan, Crina Paduraru, Taylor Blake, Arun B. Arunachalam
Summary: A robust SPR-based method using HA-specific mAbs is described for measuring HA concentration in influenza multivalent vaccines, eliminating the need for annual preparation of antisera reagents and expediting vaccine delivery. The method shows good correlation with the current potency assay and can detect degradation of HA caused by environmental factors.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Andrew Tung Yep, Yasu Takeuchi, Othmar G. Engelhardt, Simon E. Hufton
Summary: Influenza virus poses challenges due to its antigenic variability, but there are conserved epitopes that could be targeted for therapeutic development. Single domain antibodies, with their preference for specific epitopes, small size, flexibility, and stability, are well-suited to address these vulnerabilities and provide robust therapeutics for controlling influenza.
Article
Agriculture, Dairy & Animal Science
P. Martin, A. Vinet, C. Denis, C. Grohs, L. Chanteloup, D. Dozias, D. Maupetit, J. Sapa, G. Renand, F. Blanc
Summary: The study analyzed colostrum samples and calf serum from Charolais cows to assess passive immune transfer in beef cattle. Results showed higher concentrations of RID-IgG(1) in colostrum, and increased levels of RID-IgG(1) in calf serum were associated with improved survival and growth.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Immunology
Steven Rockman, Beverly Taylor, John W. McCauley, Ian G. Barr, Ray Longstaff, Ranbir Bahra
Summary: The COVID-19 pandemic has led to rapid investigation and deployment of vaccine platforms never used before. Both new and existing vaccine platforms are likely to play a crucial role in combating future pandemics, especially influenza. The field of influenza vaccine manufacturing has a long history of successes and advancements in the past decade.
Article
Agriculture, Dairy & Animal Science
I Elsohaby, J. C. Arango-Sabogal, J. T. McClure, S. Dufour, S. Buczinski, G. P. Keefe
Summary: Accurate assessment of colostrum quality is essential for calf health and future productivity in dairy farms. A study in Atlantic Canada assessed 591 colostrum samples from 42 commercial Holstein dairy herds using RID, TIR spectroscopy, and digital Brix refractometer, with different sensitivities and specificities observed for each method. The study also estimated within-herd prevalence of low-quality colostrum and recommended the use of digital Brix refractometer for improved colostrum management practices.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Agriculture, Dairy & Animal Science
A. J. Lopez, A. J. Heinrichs
Summary: Feeding maternal colostrum to newborn calves in their first hours of life is crucial for their development. Colostrum contains various nutrients and immunoglobulins, which play a significant role in building the calves' immune system. The successful transfer of passive immunity is determined by the calf's consumption of an adequate amount of immunoglobulins. Factors such as colostrum IgG concentration, quantity fed, and age of the calf at colostrum feeding influence the outcome of passive immunity. Monitoring serum IgG concentrations is recommended to assess the overall management of colostrum. Apparent efficiency of immunoglobulin absorption can be determined through direct or indirect methods and used to evaluate the prevalence of successful or failed passive immunity transfer.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Immunology
Chung Y. Cheung, Sitara Dubey, Martina Hadrovic, Christina R. Ball, Walter Ramage, Jacqueline U. McDonald, Ruth Harvey, Simon E. Hufton, Othmar G. Engelhardt
Summary: Inactivated vaccines, particularly split or subunit vaccines, are commonly used for influenza. The gold standard potency assay for these vaccines is the SRD assay, but alternative assays have been proposed. New assays should measure the amount of functional antigen, such as HA protein, and detect potency loss caused by changes to HA integrity. Our ELISA-based potency assays using cross-reactive nanobodies are sensitive to the reference antigen used, improving correspondence with the SRD assay.
Article
Obstetrics & Gynecology
Nicola Vousden, Marian Knight
Summary: Influenza during pregnancy is associated with an increased risk of hospital admission, and there is variability in policies and uptake of immunization globally. Immunization is cost-effective and safe in preventing influenza in pregnant women and their infants.
BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY
(2021)
Article
Chemistry, Analytical
Hualin Helen Li, Jenny Xu, Li He, Lynne Ireland Denny, Richard R. Rustandi, Geethanjali Dornadula, Brock Fiorito, Zhi-Qiang Zhang
Summary: Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections worldwide. Recent advances in vaccine technology have increased the likelihood of developing a safe and effective RSV vaccine. A promising approach is the development of an RSV vaccine called V171, which consists of lipids and mRNA encoding an engineered form of the RSV F protein. Preclinical and Phase I clinical trial results have shown positive outcomes, and a cell-based relative potency assay has been developed to support the Phase II development of this vaccine.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Immunology
Ian McGovern, Alina Bogdanov, Katherine Cappell, Sam Whipple, Mendel Haag
Summary: The COVID-19 pandemic has led to increased awareness and uptake of influenza vaccination, with overall vaccination rates gradually rising since 2016. In the 2020-2021 season, there was a notable increase in vaccination rates across all age groups except for those aged 65 and above, particularly among individuals under 18. Previous vaccination history was found to be associated with vaccination status in 2020-2021, with few differences observed in patient characteristics when stratified by vaccination history.
Article
Immunology
Joseph Eiden, Bram Volckaert, Oleg Rudenko, Roger Aitchison, Renee Herber, Robert Belshe, Harry Greenberg, Kathleen Coelingh, David Marshall, Yoshihiro Kawaoka, Gabriele Neumann, Pamuk Bilsel
Summary: This study demonstrates that vaccine-induced neutralizing antibodies can protect against infection and illness from different virus strains. It is the first evidence of vaccine-induced protection against a highly drifted H3N2 challenge virus.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Kelly A. S. da Costa, Joanne Marie M. Del Rosario, Matteo Ferrari, Sneha Vishwanath, Benedikt Asbach, Rebecca Kinsley, Ralf Wagner, Jonathan L. Heeney, George W. Carnell, Nigel J. Temperton
Summary: By using NA pseudoviruses and developing the pELLA assay, we successfully measured neuraminidase inhibition levels in different influenza sera and found that pELLA is more sensitive than the commercially available NA-Fluor(TM). These studies may lead to the design of more potent, longer-lasting, and broader protective vaccines that can be used in a pre-pandemic approach in combination with HA vaccines.
Article
Virology
Xiaoquan Wang, Natalia A. Ilyushina, Vladimir Y. Lugovtsev, Nicolai V. Bovin, Laura K. Couzens, Jin Gao, Raymond P. Donnelly, Maryna C. Eichelberger, Hongquan Wan
JOURNAL OF VIROLOGY
(2017)
Article
Multidisciplinary Sciences
Natalia A. Ilyushina, Vladimir Y. Lugovtsev, Anastasia P. Samsonova, Faruk G. Sheikh, Nicolai V. Bovin, Raymond P. Donnelly
Article
Multidisciplinary Sciences
Nicolette Lee, Alexey M. Khalenkov, Vladimir Y. Lugovtsev, Derek D. Ireland, Anastasia P. Samsonova, Nicolai V. Bovin, Raymond P. Donnelly, Natalia A. Ilyushina
Article
Multidisciplinary Sciences
Yulia Desheva, Ivan Sychev, Tatiana Smolonogina, Andrey Rekstin, Natalia Ilyushina, Vladimir Lugovtsev, Anastasia Samsonova, Aleksey Go, Anna Lerner
Article
Infectious Diseases
Falko Schmeisser, Rachel Friedman, Joseph Besho, Vladimir Lugovtsev, Jackeline Soto, Wei Wang, Carol Weiss, Ollie Williams, Hang Xie, Zhiping Ye, Jerry P. Weir
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2013)
Article
Biochemical Research Methods
Laura Couzens, Jin Gao, Kim Westgeest, Matthew Sandbulte, Vladimir Lugovtsev, Ron Fouchier, Maryna Eichelberger
JOURNAL OF VIROLOGICAL METHODS
(2014)
Article
Multidisciplinary Sciences
Vladimir Y. Lugovtsev, Darya Melnyk, Jerry P. Weir
Article
Immunology
Chia Chu, Vladimir Lugovtsev, Andrew Lewis, Michael Betenbaugh, Joseph Shiloach
Article
Virology
Vladimir Y. Lugovtsev, David F. Smith, Jerry P. Weir
Article
Pharmacology & Pharmacy
Simone E. Adams, Nicolette Lee, Vladimir Y. Lugovtsev, Anastasia Kan, Raymond P. Donnelly, Natalia A. Ilyushina
ANTIVIRAL RESEARCH
(2019)
Article
Microbiology
Simone E. Adams, Vladimir Y. Lugovtsev, Anastasia Kan, Nicolai V. Bovin, Raymond P. Donnelly, Natalia A. Ilyushina
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Virology
Natalia A. Ilyushina, Nicolette Lee, Vladimir Y. Lugovtsev, Anastasia Kan, Nicolai Bovin, Raymond P. Donnelly
Article
Microbiology
Brady T. Hickerson, Simone E. Adams, Subrata Barman, Lance Miller, Vladimir Y. Lugovtsev, Richard J. Webby, William L. Ince, Raymond P. Donnelly, Natalia A. Ilyushina
Summary: This study investigates the pleiotropic effects of baloxavir resistance-associated substitutions on influenza viruses. It shows that certain PA substitutions significantly reduce virus sensitivity to baloxavir and affect viral replication and interferon gene expression.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Immunology
Marie-Clotilde Bernard, Joanna Waldock, Sylvie Commandeur, Lea Strauss, Claudia Maria Trombetta, Serena Marchi, Fan Zhou, Serge van de Witte, Peter van Amsterdam, Sammy Ho, Katja Hoschler, Vladimir Lugovtsev, Jerry P. Weir, Emanuele Montomoli, Rebecca J. Cox, Othmar G. Engelhardt, Damien Friel, Ralf Wagner, Thierry Ollinger, Sophie Germain, Hanna Sediri-Schoen
Summary: This study validated a harmonized assay for quantifying anti-NA antibodies in influenza. The assay demonstrated precision, specificity, and robustness in testing influenza vaccines. Additionally, the use of a calibrator significantly improved inter-laboratory agreement.
FRONTIERS IN IMMUNOLOGY
(2022)