Article
Microbiology
Zhiran Qin, Yangyang Chen, Jianhai Yu, Xiaoen He, Xuling Liu, Li Zhu, Qinghua Wu, Chengsong Wan, Bao Zhang, Wei Zhao
Summary: This study successfully constructed a transcription template of ZIKV ZKC2 strain using infectious clones, and recovered rZKC2 strain through transfection. Two important mutation sites were identified, with significant impacts on rZKC2 recovery. Additionally, by studying an early-introduced ZIKV strain in China, a potential site for antiviral drug development was discovered.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Chang-Joo Park, Yoon-A Lee, Seung-Min Yoo, Geon A. Kim, Myung-Shin Lee, Changhoon Park
Summary: This study demonstrated the effectiveness of the modified infectious subgenomic amplicon (ISA) method in generating recombinant dengue viruses with robust replication capabilities. The study also successfully inserted a green fluorescence protein gene into the genome of dengue virus serotype 4, highlighting the efficiency of ISA in genetically modifying the virus.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Hengxing Yuan, Yaoyao Zheng, Xiaoling Yan, Hailong Wang, Youming Zhang, Jingyun Ma, Jun Fu
Summary: A streamlined approach for generating infectious BAC clones of herpesvirus was established, bypassing the tedious method of multiple rounds of plaque purification. This method enables rapid excision of BAC vector from the viral genome in mammalian cells, resulting in pure virus genetically identical to the original strain.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Microbiology
Mingyue Hu, Wan-Fei Li, Tiantian Wu, Yang Yang, Guoquan Chen, Tongling Chen, Yongchen Liu, Yaqing Mei, De Wu, Youchuan Wei, Tingrong Luo, Hong-Jie Zhang, Yi-Ping Li
Summary: This study developed an infectious clone and a subgenomic replicon of DENV-3, facilitating the study of antivirals and viral pathogenesis. A compound, C169-P1, was identified as an inhibitor of DENV replication and cell entry. This research provides important tools for the study of DENV and the development of antiviral drugs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Pharmacology & Pharmacy
Jinqian Li, Qing Zhou, Liang Rong, Dade Rong, Yang Yang, Jiawei Hao, Zhenzhen Zhang, Ling Ma, Guirong Rao, Yuanping Zhou, Fei Xiao, Chengyao Li, Haihe Wang, Yi-Ping Li
Summary: This study successfully developed two infectious clones for genotype 1b of HCV and demonstrated a novel strategy for culture adaptation of HCV isolates by utilizing a genetically close backbone sequence. The transcriptional landscape of HCV 1b-infected hepatoma cells was characterized, facilitating future studies on genotype 1b infection.
ANTIVIRAL RESEARCH
(2021)
Review
Medicine, Research & Experimental
Wen-Hung Wang, Aspiro Nayim Urbina, Chih-Yen Lin, Zih-Syuan Yang, Wanchai Assavalapsakul, Arunee Thitithanyanont, Po-Liang Lu, Yen-Hsu Chen, Sheng-Fan Wang
Summary: The development of dengue vaccines is crucial in preventing and controlling the global health threat caused by Dengue virus. Current approved vaccines have demonstrated low efficacy in certain populations, leading to ongoing investigation and development of novel strategies for dengue vaccines.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Virology
Mingyue Hu, Tiantian Wu, Yang Yang, Tongling Chen, Jiawei Hao, Youchuan Wei, Tingrong Luo, De Wu, Yi-Ping Li
Summary: In this study, a full-length infectious clone of DENV-1 genotype I isolate was successfully constructed, and the virus's infectivity and titer were improved through consecutive passages and amino acid substitutions. The clone exhibited genetic stability in transformant bacteria, serving as a useful tool for DENV-1 research.
Article
Immunology
Ploypailin Semkum, Nattarat Thangthamniyom, Penpitcha Chankeeree, Challika Keawborisuth, Sirin Theerawatanasirikul, Porntippa Lekcharoensuk
Summary: In this study, infectious clones of foot-and-mouth disease virus (FMDV) types O and A were successfully constructed using Gibson Assembly (GA) technology. These clones were generated by joining each FMDV coding region with a 4.3-kb FMDV minigenome vector. The assembled DNA was transfected into BHK-21 cells to produce the infectious FMDVs, which showed similar growth kinetics and antigenicity to the parental viruses. This method and the FMDV minigenome will facilitate the construction of FMDV infectious clones and enable genetic manipulation for FMDV research and vaccine production.
Article
Microbiology
Shuyun Li, Shunyi Fan, Nianning Li, Yuxi Shen, Xuelian Xiang, Wen Chen, Jing Xia, Xinfeng Han, Min Cui, Yong Huang
Summary: The N1038S substitution and (1154)EQTRPKKSV(1162) deletion in the S2 subunit of IBV are closely related to the replication efficiency and pathogenicity of the virus.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Chung-Hao Huang, Yu-Te Tsai, Seng-Fan Wang, Wen-Hung Wang, Yen-Hsu Chen
Summary: Development of efficient dengue vaccines is still lacking despite the presence of licensed vaccines like CYD-TDV. Efforts are being made to improve vaccine efficacy and eliminate the risk of severe dengue through heterologous prime-boost regimens. Additionally, the absence of an ideal animal model remains a challenge in dengue vaccine development.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2021)
Article
Immunology
Abid Ullah Shah, Zhisheng Wang, Yating Zheng, Rongli Guo, Saisai Chen, Mengwei Xu, Chuanjian Zhang, Yamei Liu, Jichun Wang
Summary: This study demonstrates the construction of a novel vaccine, HVT-VP2-09, which effectively combats the threat of infectious bursal disease virus (IBDV) in chickens, showing good growth and protective effects post-vaccination.
Article
Microbiology
Jasmine B. Ayers, Xuping Xie, Heather Coatsworth, Caroline J. Stephenson, Christy M. Waits, Pei-Yong Shi, Rhoel R. Dinglasan
Summary: Dengue virus, primarily transmitted by Aedes aegypti mosquitoes, has been increasing in autochthonous cases in Florida since 2009, alongside consistent importation cases. Studies reveal the potential for undetected human-mosquito transmission and long-term maintenance of the virus in mosquito populations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Hanh T. Nguyen, Qian Wang, Saumya Anang, Joseph G. Sodroski
Summary: Efforts to develop an effective HIV-1 vaccine have been frustrated by the inability to elicit broad neutralizing antibodies that recognize multiple virus strains. Such antibodies can bind a particular shape of the HIV-1 envelope glycoprotein trimer, as it exists on a viral membrane but before engaging receptors on the host cell. Here, we establish simple yet powerful assays to characterize the envelope glycoproteins in a natural context on virus particles. We find that, depending on the HIV-1 strain, some envelope glycoproteins change shape and fall apart, creating decoys that can potentially divert the host immune response. We identify requirements to keep the relevant envelope glycoprotein target for broad neutralizing antibodies intact on virus-like particles.
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Jinjin Wang, Yan Li, Yawen Zhang, Longfei Chen, Lichun Fang, Shuang Chang, Yixin Wang, Peng Zhao
Summary: This study confirmed the pathogenicity of the chicken infectious anemia virus (CIAV) detected in contaminated vaccines through reverse genetic assays. The virus caused anemia and immune suppression in poultry. This highlights the significance of vaccine contamination and the potential risks associated with it.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Tomohiro Kotaki, Yurie Nagai, Atsushi Yamanaka, Eiji Konishi, Masanori Kameoka
Summary: This study focused on developing a Japanese encephalitis (JE) vaccine that does not induce antibody-dependent enhancement (ADE) of dengue virus (DENV) infection. By introducing a mutation at the 106th amino acid residue of the JEV E protein, the modified JE vaccine was able to significantly reduce the production of cross-reactive ADE-prone antibodies against DENV.
