Article
Biochemistry & Molecular Biology
Carmen Gonzalez-Fernandez, Alberto Cuesta
Summary: Nanoplastics might have negative effects on aquatic organisms, including their disease resistance. This study found that exposure to functionalized polystyrene NPs increased viral replication and clinical symptoms of nervous necrosis virus (NNV) in fish, while also reducing their inflammatory and antiviral response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Hyun Jung Gye, Han Sol Lee, Toyohiko Nishizawa
Summary: Experimental observations suggest that sevenband grouper used in the study may have experienced mild NNV infections in the past and naturally convalesced in an aquaculture farm setting without causing mass mortality.
Article
Fisheries
M. Makesh, N. Venkata Satyanarayana, K. Muddukrishnaiah, Sujeet Kumar, G. Thiagarajan, Ashok Kumar Jangam, R. Subburaj, M. Kailasam, K. K. Vijayan
Summary: The red-spotted grouper nervous necrosis virus (RGNNV) is the most prevalent genotype among fishes in tropical and temperate regions. Inactivated and recombinant VNN vaccines produced significant immune responses in Asian seabass fingerlings and broodstock, and also demonstrated efficacy under field conditions. The vaccine can be used to vaccinate broodstock to reduce loss due to VNN in early larval stages.
Article
Fisheries
Rosemary Thwaite, Angus Li, Minami Kawasaki, Chun-han Lin, Fran Stephens, Brad Cherrie, Richard Knuckey, Matt Landos, Andrew C. Barnes
Summary: Betanodavirus or NNV continues to threaten aquaculture globally, with Australian research successfully developing a vaccine for VER in giant grouper. The study underscores the importance of environmental and dietary factors in Betanodavirus outbreaks, and highlights the usefulness of autogenous vaccine platforms in complex farm operations.
Article
Zoology
Wan-Wan Zhang, Peng Jia, Xiao-Bing Lu, Xiao-Qi Chen, Jue-Hua Weng, Kun-Tong Jia, Mei-Sheng Yi
Summary: This study found that red-spotted grouper NNV induces the formation of autophagosomes but impairs their fusion with lysosomes. It was discovered that the binding of NNV's capsid protein to the cell surface receptor HSP90ab1 contributes to the induction of autophagy. Furthermore, the study revealed that the capsid protein inhibits the interaction between HSP90ab1 and AKT, blocking the AKT-mTOR pathway.
ZOOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Han Sol Lee, Hyun Jung Gye, Toyohiko Nishizawa
Summary: In this study, the effect of NNV on SSN-1 cells was investigated in terms of adsorption ratio and infection efficiency. It was found that the CPE appearance ratio decreased by 17% for each halving of NNV infectivity dose, suggesting that this system accurately detects subtle differences in NNV infectivity. Additionally, the NNV particles were observed to be almost completely adsorbed onto cells within 4 days.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Hyun Jung Gye, Toyohiko Nishizawa
Summary: Research has shown that exposure to PBS at 37 degrees Celsius significantly reduces the antigenicity of NNV, while treatment under high pH conditions decreases both the antigenicity and infectivity of NNV. Treatment with urea reduces the antigenicity of NNV without affecting its infectivity.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Gao-Yang Liu, Zhong-Yu Zhang, Fei Ling, Peng-Fei Li, Er-Long Wang, Gao-Xue Wang
Summary: The capsid protein of Nervous necrosis virus (NNV) has been identified as a potential subunit vaccine to prevent aquaculture diseases. In this study, a peptide fragment (P4) containing important epitopes was loaded onto single-walled carbon nanotubes and induced strong and sustained immune responses in grouper.
Article
Fisheries
Myung-Hwa Jung, Sajal Kole, Sung-Ju Jung
Summary: This study aims to develop a safe and efficient inactivated (IV) vaccine against rock bream iridovirus (RBIV). The evaluation results show that saponin (Sa) acts as an adjuvant to induce antiviral immune response and protect rock bream from RBIV infection. The Sa-adjuvanted IV vaccine significantly improves fish survivability through upregulation of IL1 beta, Mx, and PKR gene transcript. It provides strong protection against reinfection with RBIV.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Sandra Souto, Emilie Merour, Alain Le Coupanec, Annie Lamoureux, Julie Bernard, Michel Bremont, Jean K. K. Millet, Stephane Biacchesi
Summary: Researchers have engineered the genome of VHSV to modify gene order and introduce an expression cassette encoding the major antigen of NNV capsid protein. Eight recombinant VHSVs (rVHSV) have been successfully recovered and tested in trout and sole, showing promising results for the development of a bivalent live attenuated vaccine candidate for the protection against VHSV and NNV.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Sergio Ortega-del Campo, Luis Diaz-Martinez, Patricia Moreno, Esther Garcia-Rosado, M. Carmen Alonso, Julia Bejar, Ana Grande-Perez
Summary: NNV is a neurotropic virus that causes viral nervous necrosis disease in fish. It has a bisegmented (+) ssRNA genome and the variability of its genome may be associated with its virulence.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Rahul Rajendran, Rahul Krishnan, Jong -Oh Kim, Myung-Joo Oh
Summary: Ion channels in fishes play an important role in the excitation and transmission of impulses through neuronal cells. This study reveals that the expression of potassium channel genes changes during NNV infection in the sevenband grouper, and the downregulation of these genes is associated with viral replication. These findings suggest that potassium channels play a crucial role during viral infection.
Article
Immunology
Zahaludin Amir-Danial, Mohd Zamri-Saad, Mohammad Noor Azmai Amal, Salleh Annas, Aslah Mohamad, Sutra Jumria, Tilusha Manchanayake, Ali Arbania, Md Yasin Ina-Salwany
Summary: This study determines the immune response and protective efficacy of a monovalent vaccine against vibriosis in Asian seabass. The results show that the vaccinated group has significantly higher innate and adaptive immune responses compared to the non-vaccinated group. This study reveals that feed-based vaccination improves growth performance, stimulates innate and adaptive immune responses, and increases protection against vibriosis in Asian seabass.
Article
Immunology
Azure Tariro Makadzange, Patricia Gundidza, Charles Lau, Norest Beta, Nellie Myburgh, Nyasha Elose, Wilmot James, Lawrence Stanberry, Chiratidzo Ndhlovu
Summary: This study evaluated the adverse events following vaccination with whole inactivated COVID-19 vaccines in an African population and found that the vaccines were well tolerated with mostly mild symptoms, and no hospitalizations were required. Participants who perceived the vaccines as safe or had a personal experience with COVID-19 were less likely to report adverse events.
Article
Immunology
Richard I. Walker, A. Louis Bourgeois
Summary: Oral immunization is an effective strategy against mucosal enteric pathogens. Inactivated whole bacterial cells can also induce protective immunity. Developing a safe and simple delivery format with controlled immunogenic amounts of complex antigens is essential. Enhancing the efficacy of whole cell vaccines can be achieved through genetic engineering and the use of mucosal adjuvants. The development of ETVAX, a vaccine candidate against enterotoxigenic Escherichia coli (ETEC), has shown promising results.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Jui-Shin Chang, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2015)
Article
Fisheries
Y. -C. Wu, P. -Y. Tsai, J. -C. Chan, S. -C. Chi
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2016)
Article
Fisheries
Tz-Shiang Chen, Yu-Chi Wu, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2016)
Article
Biophysics
Shu Kuan, Shau-Chi Chi, Yung-Jen Cheng, Ta-Jui Chia, Long-Sun Huang
BIOSENSORS & BIOELECTRONICS
(2012)
Article
Fisheries
Yu-Chi Wu, Yu-Hsuan Kai, Shau-Chi Chi
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2013)
Article
Fisheries
Yi-Da Wang, Chun-Wei Kung, Shau-Chi Chi, Jyh-Yih Chen
FISH & SHELLFISH IMMUNOLOGY
(2010)
Article
Fisheries
Ta-Jui Chia, Yu-Chi Wu, Jyh-Yih Chen, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2010)
Article
Fisheries
Yu-Chi Wu, Yi-Fan Lu, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2010)
Article
Fisheries
Yun-Tsan Chang, Yu-Hsuan Kai, Shau-Chi Chi, Yen-Ling Song
FISH & SHELLFISH IMMUNOLOGY
(2011)
Article
Fisheries
Chen-Fu Xing, Hung-Hsi Hu, Jian-Bin Huang, Han-Chun Fang, Yu-Hsuan Kai, Yu-Chi Wu, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2013)
Article
Fisheries
Yu-Hsuan Kai, Yu-Chi Wu, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2014)
Article
Fisheries
Jian-Bin Huang, Yu-Chi Wu, Shau-Chi Chi
FISH & SHELLFISH IMMUNOLOGY
(2014)
Article
Virology
Jui-Shin Chang, Shau-Chi Chi
JOURNAL OF VIROLOGY
(2015)
Article
Fisheries
Yuan-kai Cheng, Yu-Chi Wu, Shau-Chi Chi
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2017)
Article
Engineering, Multidisciplinary
Chia-Hsuan Sung, Shau-Chi Chi, Kwo-Ching Huang, Jenn-Kan Lu
JOURNAL OF MARINE SCIENCE AND TECHNOLOGY-TAIWAN
(2010)