Review
Biochemistry & Molecular Biology
Uri Galili
Summary: The alpha-gal epitope is a carbohydrate antigen synthesized by the glycosylation enzyme alpha 1,3galactosyltransferase in non-primate mammals, lemurs, and New-World monkeys, but extinct in ancestral Old-World monkeys and apes. The interaction between the alpha-gal epitope and anti-Gal in mammals can lead to hyperacute rejection during organ transplantation from non-primate mammals to Old-World monkeys or humans. Harnessing this interaction for development of novel immunotherapies (alpha-gal therapies) in various clinical settings may induce beneficial immune processes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yongxue Lv, Liangliang Chang, Jihui Yang, Jia Wen, Yinqi Zhao, Mingxing Zhu, Changyou Wu, Wei Zhao
Summary: Cutting off transmission route is an effective method to prevent Echinococcus granulosus infection, and vaccines are the most effective means of controlling and eliminating infectious diseases. This study shows that peptide vaccines based on recombinant protein P29 of E. granulosus can induce Th1-mediated cellular immune responses and high levels of specific antibodies in mice. In addition, immunization with rEg.P29(T+B) can induce higher antibody and cytokine production levels than single-epitope vaccines, and has longer immune memory. These findings suggest that rEg.P29(T+B) has the potential to be an efficient subunit vaccine for use in areas endemic to E. granulosus.
Article
Medicine, Research & Experimental
Jianmei Huang, Meiying Li, Bingjie Mei, Junyang Li, Yi Zhu, Qiaoshan Guo, Jianming Huang, Guonan Zhang
Summary: The study suggests that desialylated tumor cell vaccines can enhance tumor antigen presentation and overcome immune suppression, thereby promoting anti-tumor immunity. This research provides a new strategy for immunotherapy in ovarian cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Zhaozhen Liu, Can Pang, Yuchuan Deng, Caiping Guo, Jia Li, Yanping Li, Ruolei Xin, Xiyao Li, Conghui Xu, Chun Huang, Hongyan Lu, Jie Li
Summary: This study investigated the immunogenicity of the quadrivalent inactivated influenza vaccine in HIV-infected and uninfected individuals, and found that HIV-infected individuals had weaker immune responses, especially those with CD4+ T cell counts below 350 cells/mm3.
Article
Biochemistry & Molecular Biology
Gayathri Anandhan, Yogesh B. Narkhede, Manikandan Mohan, Premasudha Paramasivam
Summary: Respiratory syncytial virus (RSV) is a contagious viral pathogen that causes serious respiratory infection in adults and neonates. The monoclonal antibodies palivizumab and motavizumab are the only approved treatments for RSV, but they are expensive and require hospital administration. In this study, in silico prediction methods were used to identify potential vaccine targets for RSV. By analyzing the entire proteome of RSV strain A, 70 potential immunogenic epitopes were identified, including 13 experimentally verified epitopes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Aqel Albutti
Summary: The researcher conducted a study on tuberculosis, identifying two potential vaccine proteins and designing a multi-epitope vaccine with high binding affinity and stable dynamics, capable of producing abundant immunoglobulins and interferons. The experimental evaluation of this designed vaccine could potentially help control drug-resistant infections of M. tuberculosis.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Dustin M. McCraw, Mallory L. Myers, Neetu M. Gulati, Madhu Prabhakaran, Joshua Brand, Sarah Andrews, John R. Gallagher, Samantha Maldonado-Puga, Alexander J. Kim, Udana Torian, Hubza Syeda, Seyhan Boyoglu-Barnum, Masaru Kanekiyo, Adrian B. McDermott, Audray K. Harris
Summary: Structure-guided design of nanoparticles displaying conserved regions of influenza virus has the potential to develop more efficacious and universal vaccines. This design concept provides a new approach to universal influenza vaccine development strategies and opens opportunities for the development of nanoparticles with broad coverage over many antigenically diverse influenza HA subtypes.
Article
Infectious Diseases
Hannah M. Garcia Garrido, Jenny L. Schnyder, Beheshta Haydari, Albert M. Vollaard, Michael W. T. Tanck, Godelieve J. de Bree, Bob Meek, Martin P. Grobusch, Abraham Goorhuis
Summary: The immunogenicity of the combined pneumococcal vaccination schedule in PLWH was investigated, showing low initial seroprotection rates and rapid decline, highlighting the need for further research into alternative strategies and higher-valent vaccine schedules.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Immunology
Cecilia Losada, Hady Samaha, Erin M. Scherer, Bahaa Kazzi, Lana Khalil, Ighovwerha Ofotokun, Nadine Rouphael
Summary: People living with HIV (PLH) have a higher risk of HPV infection and HPV-related diseases. However, there is limited data on the long-term effectiveness of HPV vaccines in this population. PLH have lower seroconversion rates and lower antibody levels compared to HIV-negative individuals, especially those with a low CD4 count and detectable viral load. It is uncertain whether these differences affect protection. Few studies have focused on vaccine efficacy in PLH, with varied results depending on age at vaccination and baseline seropositivity. Further research is needed to understand the differences between vaccine formulations and the impact of additional doses on immune protection durability.
Article
Oncology
Kjersti Flatmark, Annette Torgunrud, Karianne G. Fleten, Ben Davidson, Hedvig Juul, Nadia Mensali, Christin Lund-Andersen, Else Marit Inderberg
Summary: Pseudomyxoma peritonei (PMP) is a rare abdominal cancer without effective treatments in non-resectable cases, but GNAS mutations are common, providing a target for therapeutic cancer vaccines. Tumor samples from PMP patients showed a pre-existing immunity towards mutated Gs alpha, but immune checkpoint molecules may inhibit antitumor T cells. This suggests that peptide vaccination with Gs alpha peptides in combination with immune checkpoint inhibition could be a potential treatment for PMP and other GNAS mutated cancers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Immunology
Shridhar Bale, Lifei Yang, Mehrdad Alirezaei, Richard Wilson, Takayuki Ota, Esmeralda D. Doyle, Christopher A. Cottrell, Javier Guenaga, Karen Tran, Wenjuan Li, Leonidas Stamatatos, David Nemazee, Andrew B. Ward, Richard T. Wyatt
Summary: Fusion of the molecular adjuvant C3d to the Env trimers improves B-cell germinal center formation and antibody responses. In mice, this fusion enhances germinal center formation, magnitude of Env-specific binding antibodies, and antibody avidity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
ZhengQing Yu, SiYing Chen, JianMei Huang, WenXi Ding, YuFeng Chen, JunZhi Su, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui Li
Summary: Eimeria spp. is a worldwide threat to the poultry industry, and there are no ideal drugs or vaccines to combat it. In this study, a multiepitope antigen named NSLC protein was designed using bioinformatics. The protective immunity of NSLC protein and the optimal adjuvant, immune route, and dosage were evaluated in laying chickens. Results showed that NSLC proteins entrapped in PLGA nanospheres were the most effective in stimulating protective immunity, and PLGA nanospheres were the optimum adjuvant. The intramucosal route with PLGA nanospheres containing 300 μg of NSLC protein was the most efficient approach to induce protective immunity against the four Eimeria species.
VETERINARY RESEARCH
(2022)
Article
Chemistry, Medicinal
Ivan Pacheco, Sandra Diaz-Sanchez, Marinela Contreras, Margarita Villar, Alejandro Cabezas-Cruz, Christian Gortazar, Jose de la Fuente
Summary: High alpha-Gal content probiotics, such as Aeromonas veronii and Pseudomonas entomophila, have been found to be safe and effective in controlling Mycobacterium marinum in fish by regulating immunity and metabolism mechanisms.
Article
Chemistry, Multidisciplinary
Elena A. Egorova, Gerda E. M. Lamers, Fazel Abdolahpur Monikh, Aimee L. Boyle, Bram Slutter, Alexander Kros
Summary: Gold nanoparticles with different shapes and sizes can have different impacts on biological processes. Our study found that smaller nanoparticles induce weaker T-cell responses, while larger spherical particles can effectively present antigens. The antigen presentation efficiency of smaller particles is lower.
Article
Multidisciplinary Sciences
Magdalena K. Scheck, Lisa Lehmann, Magdalena Zaucha, Paul Schwarzlmueller, Kristina Huber, Michael Pritsch, Giovanna Barba-Spaeth, Oliver Thorn-Seshold, Anne B. Krug, Stefan Endres, Simon Rothenfusser, Julia Thorn-Seshold
Summary: Better diagnostic and analytical methods are urgently needed for vaccine research and infection control in virology. This study presents a Fluorescence Reduction Neutralisation Test (FluoRNT) for yellow fever virus, which accurately measures neutralising antibody titres in human serum samples within 24 hours.
Article
Biochemical Research Methods
Heather P. Ostendorff, Amany Awad, Karen I. Braunschweiger, Ziying Liu, Zhi Wan, Kenneth J. Rothschild, Mark J. Lim
JOURNAL OF IMMUNOLOGICAL METHODS
(2013)
Article
Biochemical Research Methods
Zheng Wang, Mingshun Zhang, Yan Wang, Yanmei Jiao, Lu Zhang, Lin Li, Zuhu Huang, Hao Wu, Jingyun Li, Shan Lu, Shixia Wang
JOURNAL OF VIROLOGICAL METHODS
(2011)
Article
Biochemical Research Methods
Jianhui Nie, Wenbo Wang, Zhiheng Wen, Aijing Song, Kunxue Hong, Shan Lu, Ping Zhong, Jianqing Xu, Wei Kong, Jingyun Li, Hong Shang, Hong Ling, Li Ruan, Youchun Wang
JOURNAL OF VIROLOGICAL METHODS
(2012)
Article
Gastroenterology & Hepatology
Gary L. Norman, Chen-Yen Yang, Heather P. Ostendorff, Zakera Shums, Mark J. Lim, Jinjun Wang, Amany Awad, Gideon M. Hirschfield, Piotr Milkiewicz, Donald B. Bloch, Kenneth J. Rothschild, Christopher L. Bowlus, Iannis E. Adamopoulos, Patrick S. C. Leung, Harry J. Janssen, Angela C. Cheung, Catalina Coltescu, M. Eric Gershwin
LIVER INTERNATIONAL
(2015)
Article
Multidisciplinary Sciences
Guohong Ge, Shixia Wang, Yaping Han, Chunhua Zhang, Shan Lu, Zuhu Huang
Article
Biochemical Research Methods
Mark J. Lim, Ziying Liu, Karen I. Braunschweiger, Amany Awad, Kenneth J. Rothschild
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2014)
Article
Immunology
Yang Dai, Xiaoting Wang, Song Zhao, Jianxia Tang, Lu Zhang, Jianrong Dai, Mingtao Zeng, Shan Lu, Yinchang Zhu, Chuan Su
Article
Biotechnology & Applied Microbiology
Shixia Wang, Michael Kishko, Shengqin Wan, Yan Wang, Frank Brewster, Glenda E. Gray, Avye Violari, John L. Sullivan, Mohan Somasundaran, Katherine Luzuriaga, Shan Lu
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2012)
Article
Biotechnology & Applied Microbiology
Mingshun Zhang, Lu Zhang, Chunhua Zhang, Kunxue Hong, Yiming Shao, Zuhu Huang, Shixia Wang, Shan Lu
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2012)
Article
Biotechnology & Applied Microbiology
Michael Hellerstein, Yongxian Xu, Tracie Marino, Shan Lu, Hong Yi, Elizabeth R. Wright, Harriet L. Robinson
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2012)
Article
Biotechnology & Applied Microbiology
Anna Gil, Siyuan Shen, Scott Coley, Laura Gibson, Don J. Diamond, Shixia Wang, Shan Lu
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2013)
Article
Biotechnology & Applied Microbiology
Aaron Wallace, Kim West, Alan Rothman, Francis A. Ennis, Shan Lu, Shixia Wang
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2013)
Article
Biotechnology & Applied Microbiology
Ke Jin, Shixia Wang, Chunhua Zhang, Yanling Xiao, Shan Lu, Zuhu Huang
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2013)
Article
Biochemistry & Molecular Biology
Jessica Roelands, Peter J. K. Kuppen, Eiman I. Ahmed, Raghvendra Mall, Tariq Masoodi, Parul Singh, Gianni Monaco, Christophe Raynaud, Noel F. C. C. de Miranda, Luigi Ferraro, Tatiana C. Carneiro-Lobo, Najeeb Syed, Arun Rawat, Amany Awad, Julie Decock, William Mifsud, Lance D. Miller, Shimaa Sherif, Mahmoud G. Mohamed, Darawan Rinchai, Marc Van den Eynde, Rosalyn W. Sayaman, Elad Ziv, Francois Bertucci, Mahir Abdulla Petkar, Stephan Lorenz, Lisa Sara Mathew, Kun Wang, Selvasankar Murugesan, Damien Chaussabel, Alexander L. Vahrmeijer, Ena Wang, Anna Ceccarelli, Khalid A. Fakhro, Gabriele Zoppoli, Alberto Ballestrero, Rob A. E. M. Tollenaar, Francesco M. Marincola, Jerome Galon, Souhaila Al Khodor, Michele Ceccarelli, Wouter Hendrickx, Davide Bedognetti
Summary: The lack of multi-omics cancer datasets with extensive follow-up information hinders the identification of accurate biomarkers of clinical outcome. In this cohort study, comprehensive genomic analyses were performed on fresh-frozen samples from 348 patients with primary colon cancer. The study revealed a gene expression signature called Immunologic Constant of Rejection that outperformed conventional prognostic molecular biomarkers. Additionally, a favorable outcome was associated with a microbiome signature driven by Ruminococcus bromii.
Review
Immunology
Yuxin Chen, Shixia Wang, Shan Lu