Article
Environmental Sciences
Jagadish Chandra Kumar Mangu, Marios Stylianou, Per-Erik Olsson, Jana Jass
Summary: The exposure to PFAS increases susceptibility to pathogens in Caenorhabditis elegans by reducing host immunity and increasing intestinal membrane permeability, while also increasing bacterial virulence, with potential implications for humans and other animals.
ENVIRONMENTAL POLLUTION
(2022)
Article
Veterinary Sciences
Susanna Di Mauro, Joel Filipe, Alessia Facchin, Laura Roveri, Maria Filippa Addis, Valentina Monistero, Renata Piccinini, Giulia Sala, Davide Pravettoni, Clarissa Zamboni, Fabrizio Ceciliani, Cristina Lecchi
Summary: This study compared the effects of two different S. aureus strains with opposite epidemiological behaviors on peripheral bovine mononuclear cells. The results showed that the secretome produced by these strains had different impacts on cell viability and inflammatory response.
VETERINARY RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Aubin Souche, Francois Vandenesch, Anne Doleans-Jordheim, Karen Moreau
Summary: Cystic fibrosis (CF) is a severe genetic disease causing impaired lung function and premature death. Chronic infections with Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are common in CF lungs. SA and PA are able to adapt to the host immune response through various strategies, including bypassing the airway epithelia, evading recognition, modulating host immune cell proliferation, and inhibiting the immune response. These mechanisms not only benefit the bacteria but also promote coinfections in CF lungs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Mamiko Takayasu, Kouichi Hirayama, Homare Shimohata, Masaki Kobayashi, Akio Koyama
Summary: Since 1995, numerous cases of MRSA infection-associated glomerulonephritis, also known as Staphylococcus infection-associated glomerulonephritis (SAGN) or IgA-IRGN, have been reported. This disease presents as rapidly progressive glomerulonephritis or acute kidney injury with proteinuria, hematuria, and ongoing infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Rahul Chatterjee, Panchanan Sahoo, Soumya Ranjan Mahapatra, Jyotirmayee Dey, Mrinmoy Ghosh, Gajraj Singh Kushwaha, Namrata Misra, Mrutyunjay Suar, Vishakha Raina, Young-Ok Son
Summary: Staphylococcus aureus is a highly deadly Gram-positive bacterium that poses a major challenge for vaccine development. The novel vaccine candidate constructed using immunoinformatics approach shows promising immune response in worldwide populations. This chimeric vaccine has the potential to elicit both humoral and cell-mediated immune responses against the multidrug-resistant pathogen.
Article
Immunology
Rosanna Leuzzi, Margherita Bodini, Isaac P. Thomsen, Elisabetta Soldaini, Erika Bartolini, Alessandro Muzzi, Bruna Clemente, Bruno Galletti, Andrea Guido Oreste Manetti, Cinzia Giovani, Stefano Censini, Sonia Budroni, Fabiana Spensieri, Erica Borgogni, Silvia Rossi Paccani, Immaculada Margarit, Fabio Bagnoli, Giuseppe Del Giudice, Clarence B. Creech
Summary: The study identified specific cytokine and functional antibody signatures in patients with different primary invasive diseases caused by Staphylococcus aureus. These data provide insights into human responses to invasive staphylococcal infections and are important for guiding the identification of novel preventive and therapeutic interventions against S. aureus. Our findings showed distinct differences in inflammatory responses and antibody levels between patients with invasive S. aureus disease and healthy donors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Kamila R. Santos, Fernando N. Souza, Eduardo M. Ramos-Sanchez, Camila F. Batista, Luiza C. Reis, Wesley F. Fotoran, Marcos B. Heinemann, Hiro Goto, Magnus Gidlund, Adriano F. Cunha, Angelica Rosa Faria, Helida M. Andrade, Andrey P. Lage, Monica M. O. P. Cerqueira, Alice M. M. P. Della Libera
Summary: Immunization with Staphylococcus aureus recombinant proteins in mice generated memory cells and led to an increase in IL-17A(+) cells, while combined with GM-CSF DNA vaccine. This approach fostered type 3 immunity, suggesting the development of a protective immune response against S. aureus.
Article
Multidisciplinary Sciences
Xinhai Chen, Olaf Schneewind, Dominique Missiakas
Summary: This study demonstrates that modifying the Fc gamma domain of therapeutic antibodies can enhance their half-life and opsonophagocytic killing, leading to improved antibody-based immunotherapy. Additionally, staphylococci are able to reduce antibody half-life by competing for FcRn binding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Katrien Van Dyck, Felipe Viela, Marion Mathelie-Guinlet, Liesbeth Demuyser, Esther Hauben, Mary Ann Jabra-Rizk, Greetje Vande Velde, Yves F. Dufrene, Bastiaan P. Krom, Patrick Van Dijck
Summary: The study revealed the interaction between Candida albicans and Staphylococcus aureus, and their importance in bacterial dissemination, while also identifying the crucial role of the host immune response in this process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jingjing Sun, Xuansheng Lin, Yige He, Baozhong Zhang, Nan Zhou, Jian-dong Huang
Summary: In this study, engineered bacterial outer membrane vesicles (OMVs) were used to develop a multitargeting S. aureus click vaccine. The click vaccine, decorated with surface exposed SpyCatcher, can flexibly bind with various SpyTag-fused S. aureus antigens. Compared with antigens mixed with alum adjuvant, the click vaccine induced more potent antigen-specific humoral and Th1-based cellular immune response, providing protection against S. aureus infection in a mouse model.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Zhen-Zhen Liu, Yong-Jun Yang, Cheng-Kai Zhou, Shi-Qing Yan, Ke Ma, Yu Gao, Wei Chen
Summary: STING plays a crucial role in suppressing necroptosis to combat pulmonary S. aureus infection, with STING deficiency resulting in impaired bacterial clearance and increased cell death.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Kira L. Tomlinson, Tania Wong Fok Lung, Felix Dach, Medini K. Annavajhala, Stanislaw J. Gabryszewski, Ryan A. Groves, Marija Drikic, Nancy J. Francoeur, Shwetha H. Sridhar, Melissa L. Smith, Sara Khanal, Clemente J. Britto, Robert Sebra, Ian Lewis, Anne-Catrin Uhlemann, Barbara C. Kahl, Alice S. Prince, Sebastian A. Riquelme
Summary: The authors demonstrate that the pathogen Staphylococcus aureus induces a distinct airway immunometabolic response dominated by the release of itaconate. This metabolite potentiates extracellular polysaccharide synthesis and biofilm formation in S. aureus, potentially facilitating chronic infection.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Oncology
Celine M. Laumont, Brad H. Nelson
Summary: The research demonstrates that IgA antibodies produced by tumor-infiltrating B cells and plasma cells can enter tumor cells via transcytosis, inhibiting oncogenic signals and enhancing T cell-mediated cytotoxicity. These findings unveil a promising new avenue for immunotherapy.
Article
Engineering, Biomedical
Brian A. Pettygrove, Rachel M. Kratofil, Maria Alhede, Peter o. Jensen, Michelle Newton, Klaus Qvortrup, Kyler B. Pallister, Thomas Bjarnsholt, Paul Kubes, Jovanka M. Voyich, Philip S. Stewart
Summary: Biofilms formed on implanted medical devices lead to persistent infections, but the mechanisms by which bacteria evade immune clearance in the early stages are not well understood. Research shows that bacterial aggregates on implant surfaces can resist neutrophil killing, allowing them to establish biofilms and acquire tolerance to immune clearance.
Review
Immunology
Maren von Koeckritz-Blickwede, Volker Winstel
Summary: NETosis is a cellular process that promotes the formation of neutrophil extracellular traps (NETs). However, pathogens often take advantage of NETs to cause persistent infections. Staphylococcus aureus, a high-priority pathogen, catalyzes and exploits NETs during pathogenesis and recurrent infections. This article focuses on how staphylococci induce NET formation and highlights the molecular and underlying principles of NETosis. It also discusses the inflammatory potential of NET-controlled microenvironments and the adaptation and counteracting strategies evolved by S. aureus to impede NET-mediated killing.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Andi Rofian Sultan, Mehri Tavakol, Nicole A. Lemmens-den Toom, Peter D. Croughs, Nelianne J. Verkaik, Annelies Verbon, Willem J. B. van Wamel
Summary: Isothermal microcalorimetry enables real-time monitoring of the sensitivity of Staphylococcus aureus biofilms towards antibiotics, showing that conventional susceptibility assays do not accurately represent the behavior of biofilm-associated S. aureus. The calorimetry-based biofilm susceptibility assay reveals that S. aureus biofilms, both methicillin-sensitive and methicillin-resistant strains, are resistant to most tested antibiotics.
Article
Infectious Diseases
Patrick M. Meyer Sauteur, Michael L. Beeton, Soren A. Uldum, Nathalie Bossuyt, Melissa Vermeulen, Katherine Loens, Sabine Pereyre, Cecile Bebear, Darja Kese, Jessica Day, Baharak Afshar, Victoria J. Chalker, Gilbert Greub, Ran Nir-Paz, Roger Dumke
Summary: This study investigated the global incidence of Mycoplasma pneumoniae (M. pneumoniae) infections after the implementation of non-pharmaceutical interventions (NPIs) against COVID-19. The results showed a significant reduction in M. pneumoniae detection rates through direct test methods (mainly PCR) after the implementation of NPIs, while the detection rates through indirect test methods (serology) did not decrease. Furthermore, direct detections remained low worldwide regardless of lockdown or school closure periods. Some sites reported a decrease in the detection of macrolide-resistant M. pneumoniae (MRMp).
Article
Public, Environmental & Occupational Health
J. Pirzadian, T. Souhoka, M. Herweijer, L. van Heel, W. J. B. van Wamel, R. H. M. Goossens, J. A. Severin, M. C. Vos
Summary: In hospitals, sinks act as reservoirs for bacterial pathogens. A study found that when fluorescein dye was applied to the drain of hospital sinks, droplets were found in almost all wash basins, especially in those without a drain plug. Sink design considerations such as installing drain plugs, reducing dripping, and adjusting the tap position can help prevent pathogen transmission from sinks.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Pharmacology & Pharmacy
Kirby R. Lattwein, Ines Beekers, Joop J. P. Kouijzer, Mariel Leon-Grooters, Simone A. G. Langeveld, Tom van Rooij, Antonius F. W. van der Steen, Nico de Jong, Willem J. B. van Wamel, Klazina Kooiman
Summary: This study investigated the effects of ultrasound-induced microbubble oscillation on Staphylococcus aureus biofilms in real-time. It found that oscillating microbubbles resulted in bacterial dispersion, and increasing pressure and cycles led to more dispersion. The presence of an antibiotic did not significantly impact the therapeutic efficacy of sonobactericide.
Article
Medicine, General & Internal
Nelianne J. Verkaik, Yunus C. Yalcin, Hannelore I. Bax, Alina A. Constantinescu, Jasper J. Brugts, Olivier C. Manintveld, Ozcan Birim, Peter D. Croughs, Ad J. J. C. Bogers, Kadir Caliskan
Summary: This study proposes a flowchart for protocolized treatment of LVAD infections and shows promising results. However, careful consideration should be given to the use of cephalexin suppressive therapy due to the risk of infections with resistant micro-organisms.
FRONTIERS IN MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
A. R. Wahadat, W. Tanis, T. W. Galema, L. E. Swart, W. J. van Leeuwen, N. J. Verkaik, C. A. M. Schurink, B. van Dalen, S. Zoet-Nugteren, C. Gurlek, R. P. J. Budde, J. W. Roos-Hesselink
Summary: This study presents the experiences of using the Endocarditis Team (ET) in the management of infective endocarditis (IE). The results show that the ET has a significant impact on the diagnostic and therapeutic approach to IE, making it highly valuable in the management of this life-threatening disease.
NETHERLANDS HEART JOURNAL
(2023)
Article
Microbiology
Valerie O. Baede, Mehri Tavakol, Margreet C. Vos, Gwenan M. Knight, Willem J. B. van Wamel
Summary: A newly developed in vitro dehydration assay using isothermal microcalorimetry (IMC) combined with mathematical modeling was used to determine the dehydration tolerance of methicillin-resistant Staphylococcus aureus (MRSA) strains. Results showed that all MRSA strains had a reduction in survival of approximately 1 log after 1 week of dehydration. Furthermore, there was no significant difference in dehydration tolerance between epidemic and nonepidemic strains, while higher starting inocula were associated with lower reduction in survival. These findings highlight the importance of effective surface cleaning to control MRSA transmission.
MICROBIOLOGY SPECTRUM
(2022)
Letter
Infectious Diseases
Patrick M. Meyer Sauteur, Victoria J. Chalker, Christoph Berger, Ran Nir-Paz, Michael L. Beeton
Article
Infectious Diseases
Alberto Antonelli, Marco Coppi, Chaitanya Tellapragada, Badrul Hasan, Ainhize Maruri, Desiree Gijon, Fabio Morecchiato, Corne de Vogel, Annelies Verbon, Willem van Wamel, Kasper Norskov Kragh, Niels Frimodt-Moller, Rafael Canton, Christian G. Giske, Gian Maria Rossolini
Summary: This study aimed to evaluate the activity of meropenem-amikacin and meropenem-colistin combinations against multi-drug-resistant Gram-negative clinical isolates. The results showed that isothermal microcalorimetry (ITMC) showed good concordance with checkerboard broth microdilution (CKBM) assays, indicating its potential for rapid evaluation of antimicrobial combinations in vitro.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Romy D. Zwittink, Cornelia Ch Wielders, Daan W. Notermans, Nelianne J. Verkaik, Annelot F. Schoffelen, Sandra Witteveen, Varisha A. Ganesh, Angela de Haan, Jeroen Bos, Jacinta Bakker, Caroline Schneeberger-van der Linden, Ed J. Kuijper, Sabine C. de Greeff, Antoni P. A. Hendrickx
Summary: Since March 2022, multidrug-resistant organisms (MDRO) originating from Ukraine have been detected in patients in the Netherlands. Genomic surveillance revealed that the majority of these MDRO strains belong to globally spread epidemic lineages, with 60% containing New Delhi metallo-β-lactamase (NDM) genes. This highlights the importance of monitoring and addressing the increase of MDRO associated with migration and medical evacuation from Ukraine.
Article
Virology
Michele M. Molendijk, My V. T. Phan, Lonneke G. M. Bode, Nikolas Strepis, Divyae K. Prasad, Nathalie Worp, David F. Nieuwenhuijse, Claudia M. E. Schapendonk, Bouke K. H. L. Boekema, Annelies Verbon, Marion P. G. Koopmans, Miranda de Graaf, Willem J. B. van Wamel
Summary: Infections caused by antibiotic resistant Staphylococcus aureus pose a significant challenge to treatment, and phages could potentially serve as an alternative to antibiotics. However, the lack of correlation between conventional in vitro and in vivo phage susceptibility assays hinders the use of phage therapy. This study assessed the susceptibility of twelve S. aureus strains to phages using four assays, and found that microcalorimetry in human serum better mimicked in vivo conditions and could be used to predict the in vivo susceptibility of S. aureus to phages.
Article
Infectious Diseases
Valerie O. Baede, Arya Gupta, Gwenan M. Knight, Leo M. Schouls, Ken Laing, Mehri Tavakol, Anais Barray, Sake J. de Vlas, Anneke S. de Vos, Antoni P. A. Hendrickx, Madeeha Khan, Mirjam E. Kretzschmar, Willem J. B. van Wamel, Gerard Lina, Francois Vandenesch, Margreet C. Vos, Adam A. Witney, Jean-Philippe Rasigade, Jodi A. Lindsay
Summary: The MACOTRA consortium aimed to identify bacterial markers of epidemic success of MRSA isolates in Europe using a representative MRSA collection originating from France, the Netherlands and the United Kingdom. The results showed that MRSA antibiotic resistance profiles and antibiotic usage were associated with the incidence of infection and successful clonal spread, but there were variations among countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Linda J. J. Wammes, Anne F. Voor in F. 't Holt, Corne H. W. Klaassen, Margreet C. C. Vos, Nelianne J. J. Verkaik, Juliette A. Severin
Summary: This study aimed to improve the sensitivity of detecting vancomycin-resistant Enterococcus faecium (VRE) transmission and determine the time from presumed exposure to detectable colonization. Retrospective analysis of 9 VRE outbreaks showed that using the first three out of five rectal swabs identified 89% of all secondary cases, compared to only 64% with the first swab. In addition, taking one or more rectal swabs around day 9 after presumed exposure was found to be the most effective screening approach.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Amaia Garmendia Urdalleta, Mathijs Van Poll, Niamh Fahy, Janneke Witte-Bouma, Willem Van Wamel, Iulian Apachitei, Amir A. A. Zadpoor, Lidy E. E. Fratila-Apachitei, Eric Farrell
Summary: Macrophage responses are crucial for successful implant integration. This study investigated the osteoimmunomodulatory properties of titanium implants functionalized with silver nanoparticles (AgNPs). The results showed that a concentration of 0.3 g/L AgNPs in the plasma electrolytic oxidation (PEO) electrolyte optimized macrophage viability and inhibition of bacteria growth. Moreover, co-cultured human marrow stromal cells (hMSCs) could differentiate without adverse effects caused by macrophages exposed to AgNP-functionalized implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Valerie O. Baede, Michella. M. M. Voet, Tanny J. K. van der Reijden, Annelies van Wengen, Deborah. E. E. Horst-Kreft, Nicole. A. A. Lemmens-den Toom, Mehri Tavakol, Margreet. C. C. Vos, Peter. H. H. Nibbering, Willem J. B. van Wamel
Summary: Bacterial survival and interactions with human skin contribute to the success of MRSA strains. Different MRSA strains have varying survival rates and expression of immune factors on different cell models. Epidemic MRSA strains express certain virulence factors.
EPIDEMIOLOGY AND INFECTION
(2022)
