Article
Biochemistry & Molecular Biology
Ivy Kekessie, Tatiana Goncharov, Laszlo G. Komuves, Domagoj Vucic, Aimin Song
Summary: The study presents a solid-phase synthesis method for conjugating biological probes to muramyl dipeptide (MDP) to examine the biological properties of nucleotide-binding oligomerization domain-containing 2 (NOD2) receptors, potentially facilitating the development of NOD2 targeting agents. The MDP conjugates generated using this approach allow for detection of NOD2 in cell lysates and NOD2 subcellular localization, showing selective co-localization with WT NOD2 but not a variant found in Crohn's disease.
BIOORGANIC CHEMISTRY
(2021)
Review
Oncology
Eliza Iwicka, Justyna Hajtuch, Krystyna Dzierzbicka, Iwona Inkielewicz-Stepniak
Summary: This article discusses promising MDP analogs for anticancer therapy, highlighting their structural changes compared to the original MDP molecule and calling for further research to find more effective and safe anticancer treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Wysocka, Krystyna Dzierzbicka, Beata Krawczyk
Summary: The study evaluated the in vitro microbiological activity of various antibiotic derivatives against common bacteria, showing promising activity against Staphylococcus aureus and Pseudomonas aeruginosa. The results suggest that structural modifications of the compounds could serve as a basis for further drug development.
ACTA BIOCHIMICA POLONICA
(2021)
Article
Biology
Haifang Wang, Xueying Shen, Xiaojiao Zheng, Ying Pan, Qin Zhang, Zhihua Liu
Summary: Lyz1 plays an important role in mediating the immune regulatory effect of commensal bacteria through the release of Nod2 ligands, and its deficiency reduces the immune response.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Shikha Nayar, Joshua K. Morrison, Mamta Giri, Kyle Gettler, Ling-Shiang Chuang, Laura A. Walker, Huaibin M. Ko, Ephraim Kenigsberg, Subra Kugathasan, Miriam Merad, Jaime Chu, Judy H. Cho
Summary: NOD2 deficiency contributes to fibrosis and stricturing complications in Crohn's disease through dysregulated homeostasis of activated fibroblasts and macrophages, which can be ameliorated by gp130 blockade. Carriers of NOD2 risk alleles exhibit dysregulated homeostasis of activated fibroblasts and macrophages, suggesting a potential role for gp130 blockade in rescuing the activated program caused by NOD2 mutations.
Article
Oncology
Ok-Jin Park, Yeongkag Kwon, Jiseon Kim, Chaeyeon Park, Cheol-Heui Yun, Seung Hyun Han
Summary: This study found that muramyl dipeptide (MDP) can alleviate post-menopausal osteoporosis through the regulation of Wnt signaling. MDP increases bone volume and bone mineral density, promotes bone formation, and inhibits osteoclast activity. These findings have significant implications for the treatment of post-menopausal bone loss.
JOURNAL OF PATHOLOGY
(2023)
Article
Pharmacology & Pharmacy
Alexandrine L. Martel, Nya L. Fraleigh, Emilie Picard, Jordan D. Lewicky, Graham Pawelec, Hoyun Lee, Grace W. Ma, Leila Mousavifar, Rene Roy, Hoang-Thanh Le
Summary: The study demonstrates that the combination of Ara-C with DS can enhance cellular antitumor immunity and reverse immunosuppression, potentially generating immune memory and improving cancer treatment.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Christina S. Kim, Anne M. Brown, Tijana Z. Grove, Felicia A. Etzkorn
Summary: Novel consensus leucine-rich repeat proteins (CLRR4-8) based on the LRR domain of NOD-like receptors show promise in high-affinity binding to bacterial cell wall component MDP. The biphasic binding curves and docking studies suggest multi-MDP binding, indicating potential for future pathogen biosensor design.
Article
Chemistry, Medicinal
Samo Guzelj, Sanja Nabergoj, Martina Gobec, Stane Pajk, Veronika Klancic, Bram Slutter, Ruza Frkanec, Adela Stimac, Primoz Sket, Janez Plavec, Irena Mlinaric-Rascan, Ziga Jakopin
Summary: This study focused on the design, synthesis, and evaluation of novel NOD2 desmuramylpeptide agonists with improved adjuvant properties. Identified compounds 68 and 75 showed promising results both in vitro and in vivo, enhancing immune cell activity and immune response to improve vaccine efficacy.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Mia Yoshikawa, Sayuri Yamada, Miho Sugamata, Osamu Kanauchi, Yuji Morita
Summary: KW3110 is efficiently incorporated into macrophages and induces IL-10 production, possibly through a mechanism involving Dectin-2 mediated phagocytosis.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Shaowei Huang, Jinrong He, Yanping Chen, Xiaojing Wang, Yanyang Li, Yulin Su, Ruyan Wen, Xiuling Li, Guanghua Yang, Shuang Luo, Lian Zhou, Xia Luo
Summary: This study found that Huangqin decoction can regulate the intestinal flora of ulcerative colitis mice, mainly by inhibiting the NOD2-dependent pathway.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Klare L. Bersch, Kristen E. DeMeester, Rachid Zagani, Shuyuan Chen, Kimberly A. Wodzanowski, Shuzhen Liu, Siavash Mashayekh, Hans-Christian Reinecker, Catherine L. Grimes
Summary: The article discusses the impact of bacterial peptidoglycan (PG) recognition elements on the human innate immune system, as well as immunostimulatory studies using synthesized PG-based disaccharide ligands.
ACS CENTRAL SCIENCE
(2021)
Article
Immunology
Xia Luo, Xiaojing Wang, Shaowei Huang, Bo Xu, Shuang Luo, Yanyang Li, Qing Wang, Yanping Chen, Xiangliang Deng, Lingyun Liu, Lian Zhou
Summary: The study reveals that an increase in abundance and infiltration of Gram-positive bacteria in colon tissues of ulcerative colitis mice. Paeoniflorin can significantly alleviate symptoms of experimental colitis, reduce Gram-positive bacteria abundance, and decrease levels of inflammatory cytokines.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Cell Biology
Charles Maisonneuve, Derek K. L. Tsang, Elisabeth G. Foerster, Lukian Maxence Robert, Tapas Mukherjee, Dave Prescott, Ivan Tattoli, Paul Lemire, Daniel A. Winer, Shawn Winer, Catherine J. Streutker, Kaoru Geddes, Ken Cadwell, Richard L. Ferrero, Alberto Martin, Stephen E. Girardin, Dana J. Philpott
Summary: The pioneering studies in the early 1980s indicated that bacterial peptidoglycan-derived muramyl peptides (MPs) have dual functions of either stimulation or immunosuppression depending on the chronicity of exposure. This study demonstrates the immunosuppressive potential of Nod1, the bacterial sensor of diaminopimelic acid (DAP)-containing MPs, in reprogramming macrophages and inducing myeloid-derived suppressor cells (MDSCs) to maintain their immunosuppressive activity, ultimately promoting tumor progression in colorectal cancer.
Article
Dentistry, Oral Surgery & Medicine
Hidetoshi Moroto, Hiroshi Inoue, Yuto Morikawa, Hiroaki Tanimoto, Kazushi Yoshikawa, Seiji Goda, Kazuyo Yamamoto
Summary: This study investigated the effects of co-stimulation with surface reaction-type pre-reacted glass-ionomer (S-PRG) filler eluate and muramyl dipeptide (MDP) on matrix metalloproteinase (MMP)-1 production by human dental pulp fibroblast-like cells (hDPFs). The results showed that the co-stimulation enhanced MMP-1 production and induced phosphorylation of ERK 1/2. This effect was likely due to the presence of strontium.
DENTAL MATERIALS JOURNAL
(2023)
Article
Rheumatology
Maria Skrzypkowska, Mariusz Stasiak, Justyna Sakowska, Joanna Chmiel, Agata Maciejewska, Adam Bucinski, Bartosz Slominski, Piotr Trzonkowski, Piotr Luczkiewicz
Summary: The study evaluated the concentrations of specific cytokines and chemokines in patients with rheumatoid arthritis and osteoarthritis. The results showed significant differences in the concentrations of various cytokines and chemokines in the blood and synovial fluid of rheumatoid arthritis patients, even in those with low disease activity. These abnormalities may serve as indicators of disease progression.
RHEUMATOLOGY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Karolina Piekarska, Zuzanna Urban-Wojciuk, Malgorzta Kurkowiak, Iwona Pelikant-Malecka, Adriana Schumacher, Justyna Sakowska, Jan Henryk Spodnik, Lukasz Arcimowicz, Hanna Zielinska, Boguslaw Tymoniuk, Alicja Renkielska, Janusz Siebert, Ewa Slominska, Piotr Trzonkowski, Ted Hupp, Natalia Maria Marek-Trzonkowska
Summary: Transfer of mitochondria from mesenchymal stem cells to allogeneic Treg cells enhances the immunosuppressive functions of Treg cells, providing potential for the development of cell-based immunotherapies.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Karolina Wojciechowicz, Marta Spodzieja, Katarzyna A. Lisowska, Anna Wardowska
Summary: Autoimmune diseases involve the loss of immune tolerance towards autoantigens. Understanding the pathogenesis and developing effective treatments are still ongoing areas of research. Immune checkpoints, particularly the BTLA-HVEM complex, have shown potential as important regulators in various immune contexts, including autoimmunity. The complex has mainly been studied in the context of cancer but may also be a target for treating autoimmune diseases.
CELLULAR IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Maciej Zielinski, Magdalena Zalinska, Dorota Iwaszkiewicz-Grzes, Mateusz Gliwinski, Matylda Hennig, Anna Jazwinska-Curyllo, Halla Kaminska, Justyna Sakowska, Anna Woloszyn-Durkiewicz, Radoslaw Owczuk, Wojciech Mlynarski, Przemyslawa Jarosz-Chobot, Artur Bossowski, Agnieszka Szadkowska, Janusz Siebert, Malgorzata Mysliwiec, Natalia Marek-Trzonkowska, Piotr Trzonkowski
Summary: This study explored the efficacy and safety of combined treatment with Tregs and rituximab in pediatric patients with T1DM. Results showed that the combined therapy was consistently superior to monotherapy in terms of C-peptide levels and the maintenance of remission, with a higher rate of adverse events.
DIABETES OBESITY & METABOLISM
(2022)
Article
Immunology
Hanna Zielinska, Maciej Zielinski, Anna Debska-Zielkowska, Anna Dukat-Mazurek, Grazyna Moszkowska, Dorota Lewandowska, Alicja Debska-Slizien, Piotr Trzonkowski
Summary: This study found that simultaneous testing of C1q and SAB can provide in-depth analysis of alloantibodies before transplantation. There is a strong correlation between SAB and C1q for most HLA loci, except HLA-DP. Additionally, an unusual laboratory phenotype was identified, with positive C1q results but no corresponding SAB specificity.
TRANSPLANTATION PROCEEDINGS
(2022)
Review
Immunology
Justyna Sakowska, Lukasz Arcimowicz, Martyna Jankowiak, Ines Papak, Aleksandra Markiewicz, Katarzyna Dziubek, Malgorzata Kurkowiak, Sachin Kote, Karolina Kazmierczak-Siedlecka, Karol Polom, Natalia Marek-Trzonkowska, Piotr Trzonkowski
Summary: Autoimmune diseases and cancer have traditionally been seen as separate fields of research, but recent studies have shown that studying autoimmune diseases can be beneficial in the development of anti-cancer drugs. This review discusses the role of regulatory cell populations, effector molecules, genetic predisposition, and environmental factors in the loss of self-tolerance in autoimmunity and tolerance induction in cancer.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Juliusz Maksymilian Walczak, Dorota Iwaszkiewicz-Grzes, Michalina Ziomkowska, Magdalena Sliwka-Kaszynska, Mateusz Dasko, Piotr Trzonkowski, Grzegorz Cholewinski
Summary: In this study, 18 new amide derivatives and selected heterocyclic amines were synthesized as potential immunosuppressive agents. The most promising activity was observed in a benzoxazole analogue.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Geriatrics & Gerontology
Michal Cezary Czarnogorski, Justyna Sakowska, Mateusz Maziewski, Maciej Zielinski, Agnieszka Piekarska, Igor Obuchowski, Mikolaj Mlynski, Magdalena Dutka, Alicja Sadowska-Klasa, Ewa Zarzycka, Maria Bieniaszewska, Piotr Trzonkowski, Jacek M. Witkowski, Andrzej Hellmann, Katarzyna Ruckemann-Dziurdzinska, Jan M. Zaucha
Summary: This study compared the ageing characteristics between recipients and donors of allogeneic hematopoietic cell transplant (allo-HCT) and found that the rate of ageing in long-term allo-HCT recipients is not accelerated, except for some molecular features in the recipients' lymphocytes (mainly CD8(+)) that are characteristic of physiological ageing. However, a history of lower infection numbers in HCT recipients is associated with an increased percentage of NK cells.
Article
Biochemistry & Molecular Biology
Marlena Typiak, Piotr Trzonkowski, Monika Skotarczak, Anna Dubaniewicz
Summary: Sarcoidosis (SA) is a granulomatous disorder that affects the lungs, resembling tuberculosis (TB) in clinical characteristics but with different treatment approaches. By studying the phagocytic activity of monocytes in the blood of SA and TB patients using flow cytometry, we found higher phagocytic activity in SA patients and an increased frequency of Fc?RIII (CD16) receptors but a decreased frequency of CR1 (CD35) receptors compared to TB patients. This study provides insights into the pathomechanisms and differential diagnosis of SA and TB.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Martyna Tomaszewicz, Anna Ronowska, Maciej Zielinski, Agnieszka Jankowska-Kulawy, Piotr Trzonkowski
Summary: CD4(+)CD25(high)FoxP3(+) regulatory T cells (Tregs) are a subset of lymphocytes that can suppress the immune system through direct interactions with other cells and the secretion of suppressive compounds. Modulating the metabolic program of Tregs may improve or weaken their stability and function, making it a crucial tool in managing infections and autoimmunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Justyna Sakowska, Paulina Glasner, Anna Dukat-Mazurek, Anna Rydz, Maciej Zielinski, Irena Pellowska, Wojciech Biernat, Leopold Glasner, Katarzyna Michalska-Ma Lecka, Piotr Trzonkowski
Summary: The risk of graft failure increases significantly for repeated corneal transplantations due to alloimmunization and the development of memory T and B cells. The rejection is mainly mediated by local T cells, particularly the accumulation of effector memory CD4+ and CD8+ T cells. Additionally, the presence of CD4+ and CD8+ Tregs expressing Foxp3 and Helios may be insufficient for graft acceptance.
TRANSPLANT IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Klaudia Ciesielska-Figlon, Karolina Wojciechowicz, Anna Wardowska, Katarzyna Aleksandra Lisowska
Summary: Background: Nigella sativa has been traditionally used for medicinal purposes and has known antibacterial, virucidal, fungicidal, and antiparasitic properties. However, studies on its immunomodulatory effects are limited. This review examines in vitro and in vivo research supporting the role of Nigella sativa in modulating immune responses. The results suggest its potential as a therapeutic agent for regulating immune reactions.
Meeting Abstract
Immunology
M. Piotrowska, D. Iwaszkiewicz-Grzes, M. Gliwinski, P. Trzonkowski
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
J. Sakowska, M. Zielinski, P. Trzonkowski
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
Justyna Sakowska, Paulina Glasner, Anna Rydz, Anna Dukat-Mazurek, Maciej Zielinski, Piotr Trzonkowski, Leopold Glasner
