期刊
VACCINE
卷 27, 期 42, 页码 5846-5854出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.07.053
关键词
HMGB1 peptides; Chelator lipid; Stealth liposomes; Plasma membrane vesicles; Vaccines; Tumour immunotherapy
资金
- NHMRC of Australia [316949]
High-mobility group box 1 (HMGB1) protein is a nuclear binding protein which is released by monocytes and macrophages and is a potent maturation signal for dendritic cells (DCs). Synthetic HMGB1-related peptides are reported to be potent DC stimulants. Two HMGB1-related peptides, denoted as pHMGB-89 and pHMGB-106, were explored for their ability to enhance the immunogenicity of Ag-containing liposomes. pHMGB-engrafted liposomes targeted murine CD11c(+) and CD11b(+) cells in vitro and in vivo. Vaccination of mice with OVA-containing liposomes engrafted with pHMGB-89 and pHMGB-106 induced OVA-specific T cell priming and production of I(g)G1, IgG(2a) and IgG(2b) antibodies. Importantly, vaccination of mice with B16-OVA-derived plasma membrane vesicles (PMVs) engrafted with pHMGB-89 and pHMGB-106 inhibited turnout growth and metastasis, in syngeneic mice challenged with highly metastatic B16-OVA melanoma. The results show that vaccination with Ag-containing liposomes/PMVs engrafted with HMGB1 peptides could be an effective approach for developing novel vaccines and cancer immunotherapies. (C) 2009 Elsevier Ltd. All rights reserved.
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