期刊
VACCINE
卷 26, 期 40, 页码 5111-5122出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.03.089
关键词
FMDV DNA vaccine; cytokine adjuvant; cell-mediated immune responses; DC maturation
资金
- Hi-Tech R&D Program of China [2003 AA213102, 2003 AA241110]
- National Key Technology RD Program [2004BA519A39, 2006BAD06A06]
- CAU
Various approaches have been developed to improve efficacy of DNA vaccination, such as the use of plasmid expressing cytokine as a molecular adjuvant. In this study, we investigated whether co-inoculation of a construct expressing either IL-6 or TNF-alpha as the molecular adjuvant with FMDV DNA vaccine, pcD-VP1, can increase immune responses. Compared to the group immunized with pcD-VP1 alone, the co-inoculation with either molecular adjuvant induced a higher ratio of IgG2a/IgG1, higher levels of expression of IFN-gamma in CD4(+) and CD8(+) T cells, IL-4 in CD4(+) T cells, and in vivo antigen-specific cytotoxic response. Both adjuvants induced maturation of dendritic cells, suggesting a correlation between the initiating innate response and subsequent activating adaptive immune responses. Together, the results demonstrate that IL-6 and TNF-alpha used as molecular adjuvants can enhance the antigen-specific cell-mediated responses elicited by VP1 DNA vaccine. (C) 2008 Elsevier Ltd. All rights reserved.
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