Article
Immunology
Sakiko Shimizu, Ryo Tanaka, Eriko Itoh, Minami Maekawa-Matsuura, Yoichiro Iwase
Summary: Under the pandemic situation, there is a need for sufficient prophylactic vaccines. Intradermal (ID) vaccination is an effective method, but currently not widely used due to technical difficulties. This study presents the design and performance testing results of the ImmuciseTM Intradermal Injection System. The study shows that ImmuciseTM can inject drugs precisely into the ID tissues and is safe to use. An analysis comparing ID administration with standard subcutaneous (SC) administration of influenza vaccine shows that ID administration is equally or more effective up to 180 days later. These findings suggest that ImmuciseTM is an efficient device for distributing a small amount of vaccine widely during a pandemic.
Article
Immunology
Koen C. L. Beyers, Momen N. M. Rbeihat, Daniele S. Vasconcelos, Danielle Pasmans, Stijn Verwulgen, Vanessa V. J. Vankerckhoven
Summary: The recent SARS-Cov2 pandemic and mpox health emergency have increased interest in intradermal vaccination due to its dose sparing potential. VAX-ID & REG;, a novel intradermal drug delivery device, has shown to overcome challenges seen with traditional techniques and provide reliable and accurate drug delivery in the dermal layer of the skin.
Article
Chemistry, Multidisciplinary
Feng Tao, Qingsong Ye, Yimiao Chen, Lifeng Luo, Haiheng Xu, Jialong Xu, Zhuo Feng, Chao Wang, Tao Li, Yuxuan Wen, Yiqiao Hu, Hong Dong, Xiaozhi Zhao, Jinhui Wu
Summary: Loading antigens onto the surface of flagellate bacteria modified by a cationic polymer can overcome the skin barrier and actively deliver antigens in the skin, promoting the activation of dendritic cells and enhancing immune responses. This approach shows potential in cancer therapy and COVID-19 immunization.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Multidisciplinary Sciences
Alexey Rzhevskiy, Andrei Popov, Chavdar Pavlov, Yuri Anissimov, Andrei Zvyagin, Yotam Levin, Efrat Kochba
Summary: This study found that intradermal injection of anesthetic with Micronjet600 is a safe and effective option for providing rapid local anesthesia prior to peripheral venous cannulation, significantly reducing pain experienced during the procedure.
Article
Pharmacology & Pharmacy
Min Tang, Ayane Sagawa, Nodoka Inoue, Satomi Torii, Kana Tomita, Yoshiyuki Hattori
Summary: This study developed mRNA lipoplexes using the modified ethanol injection (MEI) method and evaluated their in vitro and in vivo protein expression efficiencies. By selecting different cationic lipids and neutral helper lipids, a total of 18 mRNA lipoplexes were generated, which showed high protein expression levels in cells. Moreover, systemic injection of these mRNA lipoplexes in mice resulted in high protein expression in the lungs and spleen, and induced high levels of antigen-specific antibodies. These findings suggest that the MEI method can enhance the efficiency of mRNA transfection, both in vitro and in vivo.
Article
Immunology
Alejandro M. Gomez, George (Giorgi) Babuadze, Marc-Andre Plourde-Campagna, Hiva Azizi, Alice Berger, Robert Kozak, Marc-Antoine de La Vega, X. Ara, Maedeh Naghibosadat, Marie-Edith Nepveu-Traversy, Jean Ruel, Gary P. Kobinger
Summary: In recent years, tattooing technology has shown promise in evaluating vaccines. A study evaluated a device called IONAID and found that it improved immune responses in animals and was safe to use in non-human primates.
Article
Toxicology
Seunghee Oh, Min Seong Jang, Kyung Jin Jung, Ji-Seok Han, Hyojin Lee, Areum Gil, Bohyun Jeon, Christine C. Roberts, Joel N. Maslow, Yong-Bum Kim, Kang-Hyun Han
Summary: pGO-1002, a non-viral DNA vaccine expressing spike and ORF3a antigens of SARS-CoV-2, was evaluated for potential toxicity, local tolerance, and immunogenicity in a preclinical study on rabbits. The vaccine was deemed safe and effective, supporting its future use in human clinical trials.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Engineering, Biomedical
Jinming Yu, Chaiyaporn Kuwentrai, Hua-Rui Gong, Renhao Li, Bao-zhong Zhang, Xuansheng Lin, Xiaolei Wang, Jian-Dong Huang, Chenjie Xu
Summary: This study explores the usage of cryomicroneedles for the intradermal delivery of mRNA molecules. CryoMNs successfully induce specific immune responses in the mouse model. This work is expected to provide a new delivery strategy for emerging mRNA therapeutics.
ACTA BIOMATERIALIA
(2022)
Article
Medicine, Research & Experimental
Asuka Takanashi, Colin W. Pouton, Hareth Al-Wassiti
Summary: Lipid nanoparticles (LNPs) are crucial for delivering mRNA vaccines. This study shows that LNPs not only induce immune responses in muscles, but also deliver mRNA to secondary lymphoid organs, leading to stronger adaptive immune responses.
MOLECULAR PHARMACEUTICS
(2023)
Review
Virology
Huy Quang Quach, Richard B. Kennedy
Summary: The study found that intradermal (ID) administration of influenza vaccines is more immunogenic in the elderly compared to intramuscular (IM) or subcutaneous (SC) routes, offering a potential solution to the decline in immunogenicity observed in this age group. Additionally, the main benefit of ID influenza vaccine lies in its dose-sparing effect, although it may cause more frequent but mild local adverse reactions.
Article
Immunology
Somruedee Chatsiricharoenkul, Suvimol Niyomnaitham, Harry Joshua Posen, Zheng Quan Toh, Paul V. Licciardi, Patimaporn Wongprompitak, Thaneeya Duangchinda, Pattarakul Pakchotanon, Warangkana Chantima, Kulkanya Chokephaibulkit
Summary: This study examined the safety and immunogenicity of intradermal fractional dose vaccines for COVID-19 primary series. The results showed that intradermal vaccination was safe and effective, generating immune responses. BNT162b2 had the lowest dose but the highest antibody response, while the antibody response against the omicron variant was the weakest.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kristoffer Hendel, Anders C. N. Hansen, Liora Bik, Charlotte Bagger, Martijn B. A. van Doorn, Christian Janfelt, Uffe H. Olesen, Merete Haedersdal, Catharina M. Lerche
Summary: Bleomycin is being repositioned in dermato-oncology for intralesional and intra-tumoural use. In this study, ablative fractional lasers (AFLs) and intradermal needle injections (NIs) were compared for topical drug delivery of BLM in an in vivo pig model. Both methods resulted in distinct cutaneous biodistribution patterns and pharmacokinetic profiles, with similar tolerability for skin reactions and potential for individualized approaches in a clinical setting.
Article
Pharmacology & Pharmacy
Hafsa Shahid Faizi, Lalitkumar K. Vora, Muhammad Iqbal Nasiri, Yu Wu, Deepakkumar Mishra, Qonita Kurnia Anjani, Alejandro J. Paredes, Raghu Raj Singh Thakur, Muhammad Usman Minhas, Ryan F. Donnelly
Summary: This study successfully delivered deferasirox transdermally using polymeric dissolving microneedles loaded with a nanosuspension. The novel system exhibited higher dissolution rate and drug deposition compared to traditional methods.
Article
Multidisciplinary Sciences
Venkata S. S. Bollimpelli, Pradeep B. J. Reddy, Sailaja Gangadhara, Tysheena P. P. Charles, Samantha L. L. Burton, Gregory K. K. Tharp, Tiffany M. M. Styles, Celia C. C. Labranche, Justin C. C. Smith, Amit A. A. Upadhyay, Anusmita Sahoo, Traci Legere, Ayalnesh Shiferaw, Vijayakumar Velu, Tianwei Yu, Mark Tomai, John Vasilakos, Sudhir P. P. Kasturi, George M. M. Shaw, David Montefiori, Steven E. E. Bosinger, Pamela A. A. Kozlowski, Bali Pulendran, Cynthia A. A. Derdeyn, Eric Hunter, Rama R. R. Amara
Summary: The study found that intradermal administration of a live-attenuated vaccinia-based SHIV vaccine provided protection in female macaques, while intramuscular administration did not. Both routes of vaccination induced similar levels of serum IgG with neutralizing and non-neutralizing activities. Intradermal vaccination induced greater germinal center Tfh and B cell responses, reduced the ratio of Th1 to Tfh cells in blood, and showed lower activation of monocytes and inflammasome compared to intramuscular vaccination.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Jukito Sonoda, Izuru Mizoguchi, Shinya Inoue, Aruma Watanabe, Ami Sekine, Miu Yamagishi, Satomi Miyakawa, Natsuki Yamaguchi, Eri Horio, Yasuhiro Katahira, Hideaki Hasegawa, Takashi Hasegawa, Kunihiko Yamashita, Takayuki Yoshimoto
Summary: The current worldwide mRNA vaccination against SARS-CoV-2 via intramuscular injection has provided significant protection against COVID-19. Intramuscular injection is generally safer and easier to perform on a large scale, but intradermal injection is considered superior for inducing protective immunity. To address this, needle-free jet injectors have been developed to deliver DNA, proteins, or drugs through the skin at high velocity. Among these, pyro-drive jet injectors utilizing gunpowder as a driving force have shown promise in inducing potent cellular and humoral immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
