Article
Immunology
Sabrina de Oliveira Emerick, Thais Vieira de Carvalho, Bianca Meirelles Miranda, Adriana Carneiro da Silva, Thais Viana Fialho Martins, Leandro Licursi de Oliveira, Eduardo de Almeida Marques-da-Silva
Summary: The study evaluated the immunization using different vaccines and adjuvants against L. chagasi infection, and found that the LPG3-SAP vaccine significantly reduced parasite load in both spleen and liver in mice, inducing Th1/Th17 response and IL-10 production.
Article
Microbiology
Felipe Gaitan-Albarracin, Monica Losada-Barragan, Nathalia Pinho, Renata Azevedo, Jonathan Duraes, Juan Sebastian Arcila-Barrera, Rodrigo C. Menezes, Fernanda N. Morgado, Vinicius de Frias Carvalho, Adriana Umana-Perez, Patricia Cuervo
Summary: Malnutrition may result in structural and immunological changes in the gut mucosae, leading to impaired immune response to Leishmania infantum infection. Pre-infection malnutrition exacerbates intestinal pathological alterations post-infection, promoting parasite proliferation in lymphoid tissues.
Article
Immunology
Marco Antonio Cabrera Gonzalez, Ana Alice Maia Goncalves, Jennifer Ottino, Jaqueline Costa Leite, Lucilene Aparecida Resende, Otoni Alves Melo-Junior, Patricia Silveira, Mariana Santos Cardoso, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno, Renato Lima Santos, Tatiane Furtado de Carvalho, Giani Martins Garcia, Paulo Ricardo de Oliveira Paes, Alexsandro Sobreira Galdino, Miguel Angel Chavez-Fumagalli, Marilia Martins Melo, Denise Silveira-Lemos, Olindo Assis Martins-Filho, Walderez Ornelas Dutra, Vanessa Carla Furtado Mosqueira, Rodolfo Cordeiro Giunchetti
Summary: Visceral leishmaniasis (VL) is a fatal disease caused by Leishmania infantum, and dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) is important in controlling human and CVL infections by reducing L. infantum infection. This study evaluated the potential of two polymeric nanoformulations loaded with Leishmania amazonensis antigens as a VL vaccine using golden hamsters as a model. The results showed that the vaccines had no adverse reactions and significantly reduced the parasitic load in the liver and spleen. These vaccines may be promising candidates for a VL vaccine.
Article
Biochemistry & Molecular Biology
Daniela P. Lage, Amanda S. Machado, Danniele L. Vale, Camila S. Freitas, Fernanda F. Ramos, Fernanda Ludolf, Joao A. Oliveira-da-Silva, Raquel S. Bandeira, Alessandra M. Silva, Luciana C. Simoes, Jamil S. Oliveira, Myron Christodoulides, Miguel A. Chavez-Fumagali, Bruno M. Roatt, Vivian T. Martins, Flavia P. Linhares, Jamille M. O. Cardoso, Isabela A. G. Pereira, Grasiele S. V. Tavares, Thiago A. R. Reis, Eduardo A. F. Coelho
Summary: Leishmania virulence proteins, such as the GTP-binding protein, have potential as vaccine candidates against disease. In this study, the gene encoding GTP was cloned and the recombinant protein was evaluated as a vaccine candidate against Leishmania infantum infection. The results showed that the vaccine induced a Th1-type immune response and significantly reduced parasite load in mice.
Review
Immunology
Greta Volpedo, Thalia Pacheco-Fernandez, Parna Bhattacharya, Timur Oljuskin, Ranadhir Dey, Sreenivas Gannavaram, Abhay R. Satoskar, Hira L. Nakhasi
Summary: Leishmaniasis is a disease endemic in tropical and subtropical regions transmitted by infected sand flies. The interactions between Leishmania, host immune cells, and adaptive immunity play a crucial role in disease severity and development. Besides cytokine signals, metabolic shifts in innate cells also influence immune signaling cascades in Leishmania pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Manu Kupani, Rajeev K. Pandey, Sanjana Mehrotra
Summary: Neutrophils play a crucial role in the initial defense against Leishmania parasites and their interaction with the parasites can influence the outcome of the disease. Recent research has provided new insights into the role of neutrophils in visceral leishmaniasis, as well as their early interaction with the parasite in mouse models of cutaneous leishmaniasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Veterinary Sciences
Lucy A. Parker, Lucrecia Acosta, Mariana Noel Gutierrez, Israel Cruz, Javier Nieto, Enrique Jorge Deschutter, Fernando Jorge Bornay-Llinares
Summary: The study utilized city land registry for random selection of domestic dog samples and compared with shelter dogs, showing a higher prevalence among shelter dogs. The seroprevalence among domestic dogs was significantly lower than among the abandoned dogs and the spatial distribution of infected dogs was fairly homogenous throughout the city.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Thais Lopes Valentim Di Paschoale Ostolin, Miria Rodrigues Gusmao, Fernando Augusto Siqueira Mathias, Jamille Mirelle de Oliveira Cardoso, Bruno Mendes Roatt, Rodrigo Dian de Oliveira Aguiar-Soares, Jeronimo Conceicao Ruiz, Daniela de Melo Resende, Rory Cristiane Fortes de Brito, Alexandre Barbosa Reis
Summary: This study evaluated the immunogenicity and efficacy potential of a polyepitope T-cell antigen candidate against visceral leishmaniasis in a murine model. The results showed that the vaccine increased specific T-cell proliferation and produced beneficial cytokines. Additionally, it reduced pathogen infection and parasite burden. The candidate vaccine was considered potentially valuable for visceral leishmaniasis.
CELLULAR IMMUNOLOGY
(2022)
Article
Parasitology
Livia Mendes Carvalho, Miria Rodrigues Gusmao, Ana Flavia Pereira Costa, Rory Cristiane Fortes de Brito, Rodrigo Dian de Oliveira Aguiar-Soares, Jamille Mirelle de Oliveira Cardoso, Alexandre Barbosa Reis, Claudia Martins Carneiro, Bruno Mendes Roatt
Summary: The immunochemotherapy using miltefosine along with LBSapMPL-vaccine showed promising results in controlling Leishmania infection and upregulating immunity, suggesting it as a potential alternative option for human and canine visceral leishmaniasis.
Article
Immunology
Rocio Leiva-Rebollo, Juan Gemez-Mata, Dolores Castro, Juan J. Borrego, Alejandro M. Labella
Summary: The DNA vaccine based on the major capsid protein of Lymphocystis disease virus 3 has been found to induce changes in the expression of immune-related genes in vaccinated gilthead seabream. The vaccine can effectively decrease viral replication and modulate the inflammatory and interferon response, leading to control of the infection progression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Daniela P. Lage, Danniele L. Vale, Flavia P. Linhares, Camila S. Freitas, Amanda S. Machado, Jamille M. O. Cardoso, Daysiane de Oliveira, Nathalia C. Galvani, Marcelo P. de Oliveira, Joao A. Oliveira-da-Silva, Fernanda F. Ramos, Grasiele S. Tavares, Fernanda Ludolf, Raquel S. Bandeira, Isabela A. G. Pereira, Miguel A. Chavez-Fumagalli, Bruno M. Roatt, Ricardo A. Machado-de-Avila, Myron Christodoulides, Eduardo A. F. Coelho, Vivian T. Martins
Summary: The study developed a recombinant chimeric protein ChimT based on immunogenic Leishmania proteins and evaluated its immunogenicity and protective efficacy as a potential vaccine for human visceral leishmaniasis (VL). The results showed that ChimT, when combined with adjuvants, induced a specific Th1-type immune response and significantly reduced parasite load in internal organs, indicating its promising potential as a VL vaccine candidate.
Article
Immunology
Joao Guilherme Lino da Silva, Ana Alice Maia Goncalves, Liliam Teixeira Oliveira, Giani Martins Garcia, Mauricio Azevedo Batista, Ludmila Zanandreis de Mendonca, Kelvinson Fernandes Viana, Rita de Cassia Oliveira Sant'Ana, Otoni Alves de Oliveira Melo Junior, Denise Silveira-Lemos, Walderez Ornelas Dutra, Olindo Assis Martins-Filho, Alexsandro Sobreira Galdino, Sandra Aparecida Lima de Moura, Vanessa Carla Furtado Mosqueira, Rodolfo Cordeiro Giunchetti
Summary: Recent studies suggest that antigens in microparticles can enhance the immunogenicity of antiLeishmania vaccines. This study investigates the in situ effect of chitosan-coated poly(D,L-lactic) acid submicrometric particles (SMP) as adjuvants and analyzes their inflammatory profile and toxicity. The results show that SMPs promote cell recruitment without tissue damage and stimulate early innate immune responses. They also demonstrate reduced NK cell count and impaired T and B cell subsets in mice vaccinated with SMPs encapsulating Leishmania braziliensis antigen.
Review
Immunology
Daniela E. Kirwan, Deborah L. W. Chong, Jon S. Friedland
Summary: In 2019, 10 million people developed symptomatic tuberculosis (TB) disease, resulting in 1.2 million deaths. The tissue destruction in TB is mainly mediated by enzymes, particularly matrix metalloproteinases (MMPs) secreted by leukocytes and stromal cells. Platelets are implicated in TB immunopathology, with high platelet counts in TB patients correlating with disease severity and a hypercoagulable profile. Platelets likely drive TB immunopathology by influencing other immune cells, such as monocytes, leading to increased activation markers, MMP secretion, and enhanced phagocytosis. Targeted anti-platelet agents are being considered for the treatment of TB as part of host-directed therapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Olivia C. Ihedioha, Anutr Sivakoses, Stephen M. Beverley, Diane McMahon-Pratt, Alfred L. M. Bothwell
Summary: LPG is a key virulence factor that induces DKK1 production from activated platelets and drives immune responses in Leishmania infection through TLR1/2-mediated mechanisms.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Deep Bhowmik, Achyut Bhuyan, Seshan Gunalan, Gugan Kothandan, Diwakar Kumar
Summary: In this study, a global multiepitope subunit vaccination against visceral leishmaniasis (VL) was designed using innovative immunoinformatics technique. The vaccine showed promising immunogenicity and has the potential to eradicate the disease in the future.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Daiane T. Oliveira, Adriano B. Chaves-Filho, Marcos Y. Yoshinaga, Nivia Carolina N. Paiva, Claudia M. Carneiro, Sayuri Miyamoto, William T. Festuccia, Renata Guerra-Sa
Summary: The chronic consumption of a high-sugar diet induces lipid overload, oxidative stress, and impaired fatty acid oxidation in the liver, leading to the development of nonalcoholic fatty liver disease (NAFLD). This study provides comprehensive insights into the pathogenesis of NAFLD induced by high-sugar diet consumption under energy-balanced conditions.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Review
Medical Laboratory Technology
Mariana T. Rezende, Andrea G. C. Bianchi, Claudia M. Carneiro
Summary: The study evaluated the feasibility of implementing automatic methods in cervical cancer screening, and found that AMs have advantages in diagnostic performance and reducing false-negatives, as well as reducing sample rejection rates.
DIAGNOSTIC CYTOPATHOLOGY
(2021)
Article
Engineering, Multidisciplinary
Alan M. Braga, Regis C. P. Marques, Fatima N. S. Medeiros, Jeova F. S. Rocha Neto, Andrea G. C. Bianchi, Claudia M. Carneiro, Daniela M. Ushizima
Summary: This paper introduces a novel hierarchical nuclei segmentation algorithm based on narrow band level set implementation, which achieved significant accuracy and Dice coefficient values in the segmentation of cervical cell images, and demonstrated high average accuracy in classification experiments. The results indicate the suitability of the algorithm for integration into decision support systems for cervical cell screening.
Article
Pharmacology & Pharmacy
Debora Faria Silva, Levi Eduardo Soares Reis, Marina Guimaraes Carvalho Machado, Douglas Daniel Dophine, Vinicius Roberto de Andrade, Wanderson Geraldo de Lima, Margareth Spangler Andrade, Jose Mario Carneiro Vilela, Alexandre Barbosa Reis, Gwenaelle Pound-Lana, Simone Aparecida Rezende, Vanessa Carla Furtado Mosqueira
Summary: This study investigated the use of TMX-NC as an oral treatment strategy for human visceral leishmaniasis, demonstrating its anti-parasitic efficacy in animal models and showing great promise for future oral treatments.
Article
Parasitology
Livia Mendes Carvalho, Miria Rodrigues Gusmao, Ana Flavia Pereira Costa, Rory Cristiane Fortes de Brito, Rodrigo Dian de Oliveira Aguiar-Soares, Jamille Mirelle de Oliveira Cardoso, Alexandre Barbosa Reis, Claudia Martins Carneiro, Bruno Mendes Roatt
Summary: The immunochemotherapy using miltefosine along with LBSapMPL-vaccine showed promising results in controlling Leishmania infection and upregulating immunity, suggesting it as a potential alternative option for human and canine visceral leishmaniasis.
Article
Multidisciplinary Sciences
Maria A. Duque-Correa, David Goulding, Faye H. Rodgers, Claire Cormie, Kate Rawlinson, J. Andrew Gillis, Allison J. Bancroft, Hayley M. Bennett, Magda Lotkowska, Adam J. Reid, Anneliese Speak, Paul Scott, Nicholas Redshaw, Catherine McCarthy, Cordelia Brandt, Catherine Sharpe, Caroline Ridley, Judit Gali Moya, Claudia M. Carneiro, Tobias Starborg, Kelly S. Hayes, Nancy Holroyd, Mandy Sanders, David J. Thornton, Richard K. Grencis, Matthew Berriman
Summary: Whipworms are large parasites causing chronic disease in humans and other mammals. Researchers have discovered how the larvae invade host epithelial cells, establishing infection by degrading mucus layers and creating tunnels. This interaction between the whipworm and the host triggers an immune response and tissue repair.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Alcides Castro e Silva, Americo Tristao Bernardes, Eduardo Augusto Goncalves Barbosa, Igor Aparecido Santana das Chagas, Wesley Dattilo, Alexandre Barbosa Reis, Servio Pontes Ribeiro
Summary: This article studies a version of the SIR model that takes into account the spatial localization, movement, and social isolation probabilities of individuals. The effects of virus variants and vaccination rates on pandemic dynamics are discussed. The study shows that continuous waves of infections will occur unless global vaccination is implemented.
Article
Microbiology
Bruno Mendes Roatt, Jamille Mirelle de Oliveira Cardoso, Levi Eduardo Soares Reis, Gabriel Jose Lucas Moreira, Leticia Captein Goncalves, Flavia de Souza Marques, Nadia das Dores Moreira, Paula Melo de Abreu Vieira, Rodrigo Dian de Oliveira Aguiar-Soares, Rodolfo Cordeiro Giunchetti, Alexandre Barbosa Reis
Summary: Our results demonstrate a reduction of hyperplasia in the spleen, increased expression of pro-inflammatory markers, and intense reduction of splenic parasitism in dogs treated with the LBMPL vaccine. These findings suggest that the vaccine may be an effective treatment strategy for canine visceral leishmaniasis (CVL).
Review
Medicine, General & Internal
Mayron Antonio Candia-Puma, Laura Yesenia Machaca-Luque, Brychs Milagros Roque-Pumahuanca, Alexsandro Sobreira Galdino, Rodolfo Cordeiro Giunchetti, Eduardo Antonio Ferraz Coelho, Miguel Angel Chavez-Fumagalli
Summary: This systematic review and meta-analysis examined the accuracy of diagnostic tests for Chagas disease over a thirty-year period. The results showed that ELISA and qPCR techniques had higher accuracy in diagnosing chronic and acute phases of the disease, respectively. Future studies should focus on developing new diagnostic approaches for Chagas disease.
Article
Immunology
Fernando Augusto Siqueira Mathias, Thais Lopes Valentim Di Paschoale Ostolin, Levi Eduardo Soares Reis, Jamille Mirelle de Oliveira Cardoso, Rory Cristiane Fortes De Brito, Rodrigo Dian de Oliveira Aguiar Soares, Bruno Mendes Roatt, Paula Melo de Abreu Vieira, Alexandre Barbosa Reis
Summary: This study evaluated the effect and optimal dose of adjuvant systems for a vaccine candidate against Leishmania (Viannia) braziliensis. The combination of saponin and monophosphoryl lipid A (SM) as an adjuvant system increased the production of TNF and IFN-gamma, leading to the generation of central and effector memory CD4(+) T cells. Therefore, using an adjuvant system can enhance the innate immune response and has the potential for future vaccine development.
Article
Microbiology
Daniel Silva Dias, Juliana Martins Machado, Patricia Aparecida Fernandes Ribeiro, Amanda Sanchez Machado, Fernanda Fonseca Ramos, Lais Moreira Nogueira, Ana Alice Maia Goncalves, Luana de Sousa Ramos, Isadora Braga Gandra, Flaviane Silva Coutinho, Michelli dos Santos, Jonatas Oliveira da Silva, Miguel Angel Chavez-Fumagalli, Rafael Goncalves Teixeira-Neto, Ana Thereza Chaves, Mariana Campos-da-Paz, Amanda A. Souza, Rodolfo Cordeiro Giunchetti, Sonia Maria Freitas, Sandra Lyon, Danielle Ferreira de Magalhaes-Soares, Julia Angelica Goncalves Silveira, Eduardo Sergio Silva, Eduardo Antonio Ferraz Coelho, Alexsandro Sobreira Galdino
Summary: Background: Visceral leishmaniasis (VL) is a critical public health problem in over ninety countries, and control measures in Brazil have been insufficient. A chimeric protein called rMELEISH was developed for the accurate diagnosis of VL in dogs and humans. In trials, rMELEISH showed 100% sensitivity and specificity in distinguishing VL cases from other diseases and healthy samples. These preliminary results suggest that rMELEISH has potential for future diagnostic studies in the field.
Article
Microbiology
Juliana Martins Machado, Isabela Amorim Goncalves Pereira, Ana Clara Gontijo Maia, Mariana Ferraz Chaves Francisco, Lais Moreira Nogueira, Isadora Braga Gandra, Anna Julia Ribeiro, Kamila Alves Silva, Carlos Ananias Aparecido Resende, Jonatas Oliveira da Silva, Michelli dos Santos, Ana Alice Maia Goncalves, Grasiele de Sousa Vieira Tavares, Miguel Angel Chavez-Fumagalli, Mariana Campos-da-Paz, Rodolfo Cordeiro Giunchetti, Manoel Otavio da Costa Rocha, Ana Thereza Chaves, Eduardo Antonio Ferraz Coelho, Alexsandro Sobreira Galdino
Summary: This study aimed to develop a new recombinant multiepitope protein, rTC, for the diagnosis of Chagas disease. The results showed that rTC could differentiate between indeterminate and cardiac cases, as well as cross-reactive diseases and healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These findings suggest the potential of rTC to be tested in future studies against a larger serological panel for Chagas disease diagnosis.
Review
Medicine, General & Internal
Juan Jeferson Vilca-Alosilla, Mayron Antonio Candia-Puma, Katiusca Coronel-Monje, Luis Daniel Goyzueta-Mamani, Alexsandro Sobreira Galdino, Ricardo Andrez Machado-de-Avila, Rodolfo Cordeiro Giunchetti, Eduardo Antonio Ferraz Coelho, Miguel Angel Chavez-Fumagalli
Summary: This paper presents a systematic review and meta-analysis evaluating the reliability of COVID-19 diagnostic tests. Ninety-nine scientific articles were analyzed to assess the specificity and sensitivity of these tests. Molecular tests, such as RT-PCR and CRISPR, performed better in terms of accuracy compared to serological tests. The study highlights the need to improve the performance of these diagnostic tests to effectively control COVID-19 outbreaks.
Review
Veterinary Sciences
Geralda Gabriele da Silva, Maykelin Fuentes Zaldivar, Lucilene Aparecida Resende Oliveira, Reysla Maria da Silveira Mariano, Daniel Ferreira Lair, Renata Antunes de Souza, Alexsandro Sobreira Galdino, Miguel Angel Chavez-Fumagalli, Denise da Silveira-Lemos, Walderez Ornelas Dutra, Ricardo Nascimento Araujo, Lorena Lopes Ferreira, Rodolfo Cordeiro Giunchetti
Summary: The infestation of blood-sucking mites in poultry farming has caused significant damage to the industry. This article analyzes the impact of these mites on poultry health, discusses chemical and non-chemical control methods, and explores the development of vaccines as a new approach to pest control.
VETERINARY SCIENCES
(2023)
Letter
Parasitology
Servio Pontes Ribeiro, Mariana Moncassin Vale, Jose Alexandre Felizola Diniz-Filho, Geraldo Wilson Fernandes, Alexandre Barbosa Reis, Carlos Eduardo de Viveiros Grelle
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2022)
