Review
Biochemistry & Molecular Biology
Rahul Advani, Sara Luzzi, Emma Scott, Caroline Dalgliesh, Joachim Weischenfeldt, Jennifer Munkley, David J. Elliott
Summary: This article discusses the expression of splicing regulators ESRP1 and ESRP2 in aggressively proliferating primary prostate tumors as markers of disease progression. It suggests that ESRP1 and ESRP2 act as lineage survival oncogenes in prostate cancer and highlights the need to identify the gene expression targets controlled by these regulators to develop targeted therapies.
Review
Oncology
Samantha Gogola, Michael Rejzer, Hisham F. Bahmad, Wassim Abou-Kheir, Yumna Omarzai, Robert Poppiti
Summary: Prostate cancer is the second most common cancer in men worldwide, and treatment options include radiation therapy, surgery, and active surveillance. However, in most cases, the cancer eventually becomes castration-resistant. The transition from androgen-dependent to androgen-independent state is not well understood, but epithelial-to-non-epithelial transition plays a crucial role. This review summarizes the factors and pathways involved in this transition, as well as the diagnostic and prognostic biomarkers.
Article
Engineering, Biomedical
Mugdha Pol, Hanyuan Gao, He Zhang, Olivia J. George, Joseph M. Fox, Xinqiao Jia
Summary: Synthetic matrices with dynamic presentation of cell guidance cues are engineered to mimic prostate cancer progression and metastasis. A tunable hyaluronic acid-based hydrogel platform is developed using bioorthogonal tetrazine ligation with strained alkenes. The platform allows the spontaneous formation of multicellular tumoroids and the development of cellular protrusions in response to cell adhesive peptide modification. The engineered tumor model can be utilized to identify potential molecular targets and test pharmacological inhibitors, accelerating the design of innovative strategies for cancer therapeutics.
Review
Biochemistry & Molecular Biology
Enrique A. Castellon, Sebastian Indo, Hector R. Contreras
Summary: This article explores the presence and role of cancer stem cells (CSCs) and the Epithelial-Mesenchymal transition (EMT) in prostate cancer (PCa), and finds that the stemness-mesenchymal axis is a critical process related to relapse, metastasis, and resistance. Manipulating the stemness-EMT axis genes on the androgen receptor (AR) may shed light on the effect of this axis on metastasis and castration resistance in PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Guang-Cheng Luo, Lin Chen, Jiang Fang, Zhi-Jian Yan
Summary: This study revealed that hsa_circ_0030586 is significantly upregulated in prostate cancer cells and may promote epithelial-mesenchymal transition (EMT) through the PI3K-AKT signaling pathway. Experiment results showed that interfering with hsa_circ_0030586 could inhibit tumor cell proliferation, migration, and invasion in PCa.
Article
Biochemistry & Molecular Biology
Janae D. Sweeney, Marija Debeljak, Stacy Riel, Ana Cecilia Millena, James R. Eshleman, Channing J. Paller, Valerie Odero-Marah
Summary: The research showed that SOD2 expression did not correlate with tumor aggressiveness or SOD2 genotype in prostate cancer. However, the Ala-SOD2 allele may promote EMT, leading to increased cell migration. The antioxidant MSKE could inhibit EMT mediated by Ala-SOD2 SNP, indicating promising therapeutic potential for halting prostate cancer progression.
Article
Medicine, Research & Experimental
Yao Li, Quan Li, Dujian Li, Jie Gu, Duocheng Qian, Xiaojing Qin, Yu Chen
Summary: The study found that exosomal PSGR promoted migration, invasion, stemness, and epithelial-mesenchymal transitions of prostate cancer cells, and reshaped the mRNA profiles of the cells.
Review
Oncology
Xiaobo Zheng, Fuzhen Dai, Lei Feng, Hong Zou, Li Feng, Mingqing Xu
Summary: The interplay between epithelial-mesenchymal plasticity and cancer stem cells plays a crucial role in cancer progression, affecting the aggressiveness of tumor cells and their response to treatment. Understanding the dynamic nature of EMT as a hybrid state rather than a binary model is important for unraveling the complexities of cancer biology and developing targeted therapeutic interventions.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Dan Dong, Lei Na, Kailing Zhou, Zhuo Wang, Yu Sun, Qianqian Zheng, Jian Gao, Chenghai Zhao, Wei Wang
Summary: The research revealed that FZD5 prevents EMT in gastric cancer and is associated with longer survival. ELF3 acts as a downstream effector of FZD5, repressing ZEB1 expression and guarding against EMT.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Lee Armstrong, Colin E. Willoughby, Declan J. Mckenna
Summary: This study investigated the association between miR-143-3p and EMT in prostate cancer. The findings showed that miR-143-3p expression was significantly decreased in prostate cancer cells and it was associated with the EMT-associated gene AKT1. Further analysis suggested that miR-143-3p may serve as a predictor of disease recurrence in prostate cancer, making it a potential diagnostic and prognostic biomarker.
Review
Oncology
Barbora Kvokackova, Jan Remsik, Mohit Kumar Jolly, Karel Soucek
Summary: Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) play critical roles in the development of TNBC, shaping aggressive and heterogeneous biological features, but may also contribute to the progression of metastatic disease.
Article
Biochemistry & Molecular Biology
Emilia Bevacqua, Manuela Curcio, Federica Saletta, Orazio Vittorio, Giuseppe Cirillo, Paola Tucci
Summary: The study demonstrates the enhanced efficacy of curcumin when immobilized in nanoparticles, as well as the synergistic effect with Doxorubicin for prostate cancer treatment. In vitro data provides a proof of principle for the design of an effective therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Bingfeng Luo, Yuan Yuan, Yifei Zhu, Songwu Liang, Runan Dong, Jian Hou, Ping Li, Yaping Xing, Zhenquan Lu, Richard Lo, Guan-Ming Kuang
Summary: miRNA-145-5p was found to negatively regulate EMT, inhibit Pca bone metastasis, and promote apoptosis. It may serve as a potential target for treating Pca with bone metastases.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qi Chen, Meng Gu, Zhi-kang Cai, Hu Zhao, Shi-cheng Sun, Chong Liu, Ming Zhan, Yan-bo Chen, Zhong Wang
Summary: CD44 serves as a marker of cancer stem cells in many tumors, with alternative splicing generating various isoforms. This study reveals a novel mechanism where TGF-beta 1 induces CD44 alternative splicing, promoting epithelial-to-mesenchymal transition and stemness in prostate cancer cells. The findings demonstrate the important roles of CD44 isoforms and TGF-beta 1 in tumor progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Medicine, General & Internal
Yu Fan, Tu-Run Song, Qiang Wei, Lu Yang, Tao Lin, Xiao-Bing Feng, Xian-Ding Wang, Zhong-Li Huang, Shi-Jian Feng
Summary: Estrogen plays a role in the development of benign prostatic hyperplasia (BPH) through promoting AQP5 expression and subsequent epithelial-mesenchymal transition (EMT) in prostate cells. This study found that BPH tissues had higher AQP5 expression and EMT levels compared to normal prostate tissues. Estrogen-stimulated cells had increased AQP5 expression, cell proliferation, and EMT, which could be partially reversed by AQP5 knockdown.
CHINESE MEDICAL JOURNAL
(2021)
Article
Medicine, Research & Experimental
Nancy Farfan, Octavio Orellana-Serradell, Daniela Herrera, Dominique Chrzanowsky, Paulina Cubillos, Gabriel Marin, Antonio Garcia De Herreros, Enrique A. Castellon, Hector R. Contreras
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Ana F. Castillo, Ulises D. Orlando, Paula M. Maloberti, Jesica G. Prada, Melina A. Dattilo, Angela R. Solano, Maria M. Bigi, Mayra A. Rios Medrano, Maria T. Torres, Sebastian Indo, Graciela Caroca, Hector R. Contreras, Belkis E. Marelli, Facundo J. Salinas, Natalia R. Salvetti, Hugo H. Ortega, Pablo Lorenzano Menna, Sergio Szajnman, Daniel E. Gomez, Juan B. Rodriguez, Ernesto J. Podesta
Summary: ACSL4 is involved in tumor aggressiveness in breast and prostate tumors, and the inhibitor PRGL493 blocks tumor growth and sensitizes cells to chemotherapy and hormonal treatment. Additionally, PRGL493 inhibits de novo steroid synthesis in cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Paula I. Escalante, Luis A. Quinones, Hector R. Contreras
Summary: The FOLFOX scheme is commonly used for metastatic colorectal cancer patients, but chemoresistance is a major challenge. EMT and overexpression of multiple genes may contribute to drug resistance.
Article
Oncology
Gisella Perez, Fernanda Lopez-Moncada, Sebastian Indo, Maria Jose Torres, Enrique A. Castellon, Hector R. Contreras
Summary: This study demonstrated that stable silencing of ZEB1 in prostate cancer cell lines can reverse CSC properties, including decreased expression levels of CSC phenotype markers, reduced ability to form prostatospheres, and generate colonies. Targeting ZEB1 may be a key factor to eradicate CSCs and improve the prognosis of patients with advanced PCa.
Article
Biology
Catherine Sanchez, Alejandro Mercado, Hector R. Contreras, Felipe Carvajal, Apolo Salgado, Christian Huidobro, Enrique A. Castellon
Summary: The study showed that pretreatment with leuprolide and IN3 enhanced apoptosis and decreased cell survival in prostate cancer cells after radiation therapy. There was also an increased proportion of cells arrested in G2 phase.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Article
Cell Biology
Pedro Carriere, Natalia Calvo, Maria Belen Novoa Diaz, Fernanda Lopez-Moncada, Alexander Herrera, Maria Jose Torres, Exequiel Alonso, Norberto Ariel Gandini, Graciela Gigola, Hector R. Contreras, Claudia Gentili
Summary: The study shows that PTHrP modulates factors and morphological changes associated with EMT in HCT116 cells from CRC, suggesting a novel action on CRC progression involving the microenvironment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Oncology
Maria Jose Torres, Fernanda Lopez-Moncada, Daniela Herrera, Sebastian Indo, Alejandro Lefian, Paola Llanos, Julio Tapia, Enrique A. Castellon, Hector R. Contreras
Summary: The study found higher levels of ETAR expression in tissues from PCa patients with a low Gleason Score, while ETBR showed no significant changes; PC3 cells expressed higher levels of ET-1 and ETAR, while LNCaP cells exhibited higher levels of ETBR; Blocking ETAR and ETBR reduced the expression levels of steroidogenesis enzymes and AR in PC3 cells, leading to decreased T secretion.
Article
Biochemistry & Molecular Biology
Sebastian Morales-Pison, Lilian Jara, Valentina Carrasco, Cristian Gutierrez-Vera, Jose Miguel Reyes, Patricio Gonzalez-Hormazabal, Leandro J. Carreno, Julio C. Tapia, Hector R. Contreras
Summary: The rs6505162:A allele plays an important role in mammary tumorigenesis by promoting breast cancer cell proliferation, chemoresistance, migration, invasion, and inhibiting apoptosis. This finding may have implications for early diagnosis, prognosis, and treatment of breast cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Enrique A. Castellon, Sebastian Indo, Hector R. Contreras
Summary: This article explores the presence and role of cancer stem cells (CSCs) and the Epithelial-Mesenchymal transition (EMT) in prostate cancer (PCa), and finds that the stemness-mesenchymal axis is a critical process related to relapse, metastasis, and resistance. Manipulating the stemness-EMT axis genes on the androgen receptor (AR) may shed light on the effect of this axis on metastasis and castration resistance in PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Diego Figueroa, Javiera Rios, Oscar F. Araneda, Hector R. Contreras, Miguel L. Concha, Carlos Garcia
Summary: This study aimed to determine the acute toxicity of OA and DTX-1 on oxidative stress parameters in medaka larvae. The results showed that OA and DTX-1 significantly affected the levels of MDA and carbonyl content. DTX-1 exhibited a greater inhibition of the antioxidant system, leading to increased oxidative damage in medaka larvae.