Minimal genome: Worthwhile or worthless efforts toward being smaller?

Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium

(2015) M. Lluch-Senar et al.   Molecular Systems Biology

A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

(2015) Howon Lee et al.   Nature Communications

Enhancing recombinant protein production with an Escherichia coli host strain lacking insertion sequences

(2014) Myung Keun Park et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

The genome-scale interplay amongst xenogene silencing, stress response and chromosome architecture in Escherichia coli

(2014) Rajalakshmi Srinivasan et al.   NUCLEIC ACIDS RESEARCH

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual Symbioses in a Phloem-Feeding Insect

(2013) Gordon M. Bennett et al.   Genome Biology and Evolution

Microbial production of short-chain alkanes

(2013) Yong Jun Choi et al.   NATURE

Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli

(2013) Seung Min Yoo et al.   Nature Protocols

Minimal genome encoding proteins with constrained amino acid repertoire

(2013) O. Tsoy et al.   NUCLEIC ACIDS RESEARCH

Probing the Limits of Genetic Recoding in Essential Genes

(2013) M. J. Lajoie et al.   SCIENCE

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Genome Scanning for Conditionally Essential Genes in Salmonella enterica Serotype Typhimurium

(2012) Anita Khatiwara et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genome-scale promoter engineering by coselection MAGE

(2012) Harris H Wang et al.   NATURE METHODS

The essential genome of a bacterium

(2011) B. Christen et al.   Molecular Systems Biology

Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq

(2011) L. A. Gallagher et al.   mBio

Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications

(2010) Kinga Umenhoffer et al.   Microbial Cell Factories

Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

(2010) Dong-Uk Kim et al.   NATURE BIOTECHNOLOGY

Chemical synthesis of the mouse mitochondrial genome

(2010) Daniel G Gibson et al.   NATURE METHODS

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production

(2009) Jun Lee et al.   Microbial Cell Factories

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology

(2008) V. Deepak et al.   BIORESOURCE TECHNOLOGY

Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering

(2008) Carlos Olano et al.   METABOLIC ENGINEERING