Article
Materials Science, Multidisciplinary
Zhiming Zhang, Zijie Rong, Guofeng Wu, Yihan Wang, Zhiwen Tan, Juan Zheng, Yanglei Jin, Zhihao Liang, Chun Liu, Jiasong Guo, Lixin Zhu
Summary: The study introduced a controlled-oxygen-generating composite scaffold to enhance seed cell survival and improve bone defect reconstruction.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Applied
Antonia Ressler, Maja Antunovic, Laura Teruel-Biosca, Gloria Gallego Ferrer, Slaven Babic, Inga Urlic, Marica Ivankovic, Hrvoje Ivankovic
Summary: Ionic substitutions in composite materials have shown promising potential in enhancing the biological performance for bone tissue engineering applications. This study focused on multi-substituted organic/inorganic scaffolds and demonstrated that these scaffolds have high stability and can promote osteogenic potential of human mesenchymal stem cells. Results highlighted the beneficial effect of ionic substitutions on cells and tissues.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemical Research Methods
Gordian Born, Evelia Plantier, Guido Nannini, Alessandro Caimi, Andrea Mazzoleni, M. Adelaide Asnaghi, Manuele G. Muraro, Arnaud Scherberich, Ivan Martin, Andres Garcia-Garcia
Summary: Bioreactors that allow direct perfusion of cell suspensions or culture media through 3D scaffolds are widely used in tissue engineering to improve cell seeding efficiency, uniformity of cell distribution, and tissue development. A macro-scale U-shaped bioreactor has been previously developed, but the perfusion flow in the peripheral regions was not uniform. In this study, the design of the perfusion chamber has been optimized to provide more homogeneous perfusion flow through the scaffold. Furthermore, a miniaturized version of the flow-optimized bioreactor has been proposed for medium-throughput assays.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Biomedical
M. Bouyer, C. Garot, P. Machillot, J. Vollaire, V Fitzpatrick, S. Morand, J. Boutonnat, V Josserand, G. Bettega, C. Picart
Summary: The study suggests that using 3D-printed PLA scaffolds loaded with reduced doses of BMP-2 may be a safe and simple solution for large bone defects in the clinic. The dose of BMP-2 delivered from the scaffold significantly influences the amount of regenerated bone and the repair kinetics, showing good bone repair outcomes.
MATERIALS TODAY BIO
(2021)
Article
Nanoscience & Nanotechnology
Sophia Szymkowiak, Nathan Sandler, David L. Kaplan
Summary: Chronic kidney disease and kidney failure are increasing globally, with a lack of corresponding improvement in available therapies. Using silk fibroin protein scaffolds to support kidney cells can mimic the structure of the kidney and support a larger number of cells with more even distribution under perfused conditions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Martina Tortorici, Ansgar Petersen, Klara Ehrhart, Georg N. Duda, Sara Checa
Summary: Mechanical and biological factors are known to play key roles in osteochondral defect healing, but the underlying principles and how they can be applied in treatment strategies are still largely unknown. A computational approach was used to investigate the impact of scaffold mechanical and architectural properties on osteochondral defect healing, revealing that scaffold material elastic modulus and structural stability are crucial for supporting the repair process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Duoyi Zhao, Tongtong Zhu, Jie Li, Liguo Cui, Zhiyu Zhang, Xiuli Zhuang, Jianxun Ding
Summary: This article comprehensively reviews the research and applications of artificial bone-substitute materials based on PLGA and its composites, discussing their potential and challenges in bone regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Min-Chia Chen, Hsien-Chung Chiu, Po-Jan Kuo, Cheng-Yang Chiang, Martin M. Fu, Earl Fu
Summary: The study found that implanting a 900mm pore size 3D-printed PCL scaffold can facilitate new bone formation in critical-size calvarial defects, while adding PRF did not provide additional advantages.
JOURNAL OF DENTAL SCIENCES
(2021)
Article
Cell Biology
Gustavo Henrique Dona Rodrigues Almeida, Leandro Norberto da Silva-Junior, Mariana Sversut Gibin, Henrique dos Santos, Bianca de Oliveira Horvath-Pereira, Leticia Beatriz Mazo Pinho, Mauro Luciano Baesso, Francielle Sato, Luzmarina Hernandes, Charles R. Long, Luciana Relly, Maria Angelica Miglino, Ana Claudia Oliveira Carreira
Summary: This study aimed to establish and standardize a protocol for porcine ovaries' decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. Results showed that the proposed method was able to remove cellular components efficiently, and the scaffolds had preserved ECM composition, were cytocompatible, and allowed cell attachment.
Article
Materials Science, Textiles
Amir Nahanmoghadam, Maryam Asemani, Vahabodin Goodarzi, Somayeh Ebrahimi-Barough
Summary: A composite scaffold incorporating PHBV and PCL polymers with 5% HA nanoparticles was successfully fabricated for bone critical size defect regeneration. The scaffold showed enhanced bone regeneration and decreased fibrous connective tissue formation compared to other groups.
FIBERS AND POLYMERS
(2021)
Article
Engineering, Biomedical
Mahboubeh Sadat Mousavi, Ghassem Amoabediny, Seyed Hossein Mahfouzi, Seyed Hamid Safiabadi Tali
Summary: The study introduced a novel perfusion-based bioreactor (PBB) method for efficient decellularization of bovine articular cartilages, resulting in better preservation of the tissue structure and essential components while reducing harmful physical and chemical steps and process duration. The PBB method showed a 90% reduction in DNA content, significantly higher than the control method, and maintained around 50% of sulfated glycosaminoglycan (sGAG) content and 92% of compression properties, demonstrating efficient preservation of scaffold structure and components. Additionally, the PBB specimens supported cell adherence and proliferation effectively, showing promise for producing close-to-natural scaffolds for cartilage tissue engineering.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Lingjun Wang, Jiannan Mao, Feng Cai, Jincheng Tang, Kun Xi, Yu Feng, Yichang Xu, Xiao Liang, Yong Gu, Liang Chen
Summary: This study successfully guided the regeneration of critical-size bone defects on long bones and restored the natural bone anatomical structure through the construction of a scaffold with heterogeneous biomimetic structure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yunsheng Dong, Hui Xiao, Jie Wang, Tingting Yang, Naiqi Kang, Jiaxing Huang, Wei Cui, Yufei Liu, Qiang Yang, Shufang Wang
Summary: Constructing scaffolds with osteogenic activity through tissue engineering is an effective strategy for bone regeneration. This study used gelatin, silk fibroin, and Si3N4 to prepare silicon-functionalized bio-macromolecules composite scaffolds using 3DP technology. The scaffold showed good cyto-compatibility and promoted osteogenic differentiation of MSCs in vitro, and facilitated bone regeneration in vivo. The composite scaffold system has great potential for application in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Mathematics, Applied
Prakash Kumar, G. P. Raja Sekhar
Summary: This paper develops a mathematical model to study fluid flow, nutrient transport, and cell growth inside a bioreactor. The model includes deformable scaffold material, biphasic mixture theory equations, Stokes equation for flow, advection-diffusion-reaction equation for nutrient balance, Contois equation for cell growth, and the use of Laplace transformation to handle time-dependent terms. The study investigates nutrient outreach in the scaffold region, factors affecting nutrient concentration, and computes total mass transfer rate for nutrient distribution across different regions of the bioreactor.
MATHEMATICAL METHODS IN THE APPLIED SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Rui Du, Bin Zhao, Kun Luo, Man-Xi Wang, Quan Yuan, Lei-Xiao Yu, Ke-Ke Yang, Yu-Zhong Wang
Summary: In this study, a bioactive and shape memory bone scaffold was fabricated using a four-dimensional fused deposition modeling technique. The scaffold utilized a poly(e-caprolactone) segment with a transition temperature near body temperature as the molecular switch for shape memory effect, and a poly(propylene fumarate) segment for improving regulation effect on osteogenesis. The experimental results demonstrated that the scaffold could quickly adapt to defect geometry through shape change, and significantly promote bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Claudia Kleinhans, Lena Schmohl, Jakob Barz, Petra J. Kluger
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Nanoscience & Nanotechnology
Silke Keller, Katharina Woergoetter, Anke Liedek, Petra J. Kluger, Monica Bach, Guenter E. M. Tovar, Alexander Southan
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Engineering, Biomedical
Silke Keller, Tomke Bakker, Benjamin Kimmel, Lisa Rebers, Tobias Goetz, Guenter E. M. Tovar, Petra J. Kluger, Alexander Southan
Summary: By functionalizing gelatin with azides, it can be covalently immobilized in hydrogels, improving the efficiency of using biological materials. Experimentally validated, this new gelatin derivative is not only noncytotoxic to cells, but also significantly enhances cell adhesion on the material surface.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Multidisciplinary Sciences
A. Leucht, A-C Volz, J. Rogal, K. Borchers, P. J. Kluger
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Freia F. Schmidt, Sophia Nowakowski, Petra J. Kluger
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Svenja Nellinger, Isabelle Schmidt, Simon Heine, Ann-Cathrin Volz, Petra J. Kluger
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biochemistry & Molecular Biology
Svenja Nellinger, Mareike A. Rapp, Alexander Southan, Valentin Wittmann, Petra J. Kluger
Summary: Extracellular matrix (ECM) serves as the natural environment for cells and can be specifically modified with functional groups through metabolic glycoengineering without altering its structure. This allows for further functionalization in various applications without affecting the cellular behavior.
Article
Chemistry, Multidisciplinary
Max Wacker, Jan Riedel, Heike Walles, Maximilian Scherner, George Awad, Sam Varghese, Sebastian Schuerlein, Bernd Garke, Priya Veluswamy, Jens Wippermann, Joern Huelsmann
Summary: This study contrasted the effects of different surface coatings on the growth of human cells on bacterial nanocellulose small-diameter vascular grafts. It was found that fibronectin and heparin-chitosan coatings could positively impact endothelialization, potentially improving the longevity and reducing thrombogenicity of the vascular grafts in the future.
Article
Biotechnology & Applied Microbiology
Svenja Nellinger, Ivana Mrsic, Silke Keller, Simon Heine, Alexander Southan, Monika Bach, Ann-Cathrin Volz, Thomas Chasse, Petra J. Kluger
Summary: This study compared and characterized human adipose tissue-derived dECM and cdECM, revealing differences in molecular composition, structural characteristics, and biological purity. These findings can aid researchers in selecting suitable ECM-based biomaterials for specific applications and interpreting experimental results.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Pharmacology & Pharmacy
Cornelia Wiese-Rischke, Rasika S. Murkar, Heike Walles
Summary: The review summarizes the development and application of airway models, ranging from cell line models to organoids. The function of mucus as a barrier component and the need for nondestructive characterization of barrier models using TEER measurements and live cell imaging are emphasized. Organ-on-a-chip technology presents a great opportunity for cultivating complex airway models.
Review
Biochemistry & Molecular Biology
Svenja Nellinger, Petra Juliane Kluger
Summary: Adipose-derived stem cells (ASCs) are a promising cell type in tissue engineering and medical approaches. They can be easily obtained and expanded, and have the ability to differentiate into clinically relevant cell types. The extracellular matrix (ECM) surrounding cells provides physical and chemical cues that influence ASC behavior. This review provides an overview of current research on ASC mechanosensing and the impact of biomaterial properties on ASC behavior, including stiffness, topography, and chemical modification. The use of natural ECM as a biomaterial and its interaction with ASC behavior are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Franziska B. Albrecht, Freia F. Schmidt, Ann-Cathrin Volz, Petra J. Kluger
Summary: This study investigates the relationship between adipose tissue and multiple diseases, highlighting the importance of suitable in vitro models for research purposes. The researchers successfully developed physiological in vitro adipose tissue models using bioprinting technology.
Article
Polymer Science
Franziska B. Albrecht, Vera Dolderer, Svenja Nellinger, Freia F. Schmidt, Petra J. Kluger
Summary: GG-based adipose tissue models demonstrate long-term stability and facilitate the maturation and functionality of adipocytes.
Article
Polymer Science
Jannis O. Wollschlaeger, Robin Maatz, Franziska B. Albrecht, Annemarie Klatt, Simon Heine, Andreas Blaeser, Petra J. Kluger
Summary: With the growing world population, finding alternative ways to meet the demand for meat is becoming increasingly important. This study evaluated agarose, gellan, and a xanthanlocust bean gum blend (XLB) as support materials for cultured meat. The addition of pea and soy protein to the support materials was also analyzed. The results showed that these blends have the potential for cultured meat production, but high protein content led to unstable gels. Agarose and gellan blends had suitable gelation temperature ranges, while XLB blends required higher temperatures. The polysaccharide-protein gels exhibited different viscosity and swelling behavior compared to pure polysaccharide gels. None of the investigated materials showed cytotoxic effects on the myoblast cell line C2C12. Gellan blends were found to be the most suitable for cell-laden gels, followed by agarose blends, while stable cell-laden gels could not be formed with XLB blends.
Article
Chemistry, Multidisciplinary
Silke Keller, Anke Liedek, Dalia Shendi, Monika Bach, Gunter E. M. Tovar, Petra J. Kluger, Alexander Southan