Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications

Manipulating the substrate specificity of murine dihydrofolate reductase enzyme using an expanded set of amino acids

(2015) Shun Zheng et al.   BIOCHEMICAL ENGINEERING JOURNAL

Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells

(2015) Michael Ehrlich et al.   CHEMISTRY-A EUROPEAN JOURNAL

Bioconjugation of therapeutic proteins and enzymes using the expanded set of genetically encoded amino acids

(2015) Sung In Lim et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Conceptual and methodological advances in cell-free directed evolution

(2015) Igor Dodevski et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization

(2015) Jeffrey Chun Yu Wu et al.   JOURNAL OF BIOTECHNOLOGY

Unnatural amino acid mutagenesis-based enzyme engineering

(2015) Yuvaraj Ravikumar et al.   TRENDS IN BIOTECHNOLOGY

Significant improvement of oxidase activity through the genetic incorporation of a redox-active unnatural amino acid

(2015) Yang Yu et al.   Chemical Science

Designing logical codon reassignment – Expanding the chemistry in biology

(2015) Anaëlle Dumas et al.   Chemical Science

Dissecting Ubiquitin Signaling with Linkage-Defined and Protease Resistant Ubiquitin Chains

(2014) Tatjana Schneider et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Overview on the biotechnological production of l-DOPA

(2014) Kyoungseon Min et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recent advances in engineering proteins for biocatalysis

(2014) Ye Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Therapeutic Applications of an Expanded Genetic Code

(2014) Sophie B. Sun et al.   CHEMBIOCHEM

Optimized Plasmid Systems for the Incorporation of Multiple Different Unnatural Amino Acids by Evolved Orthogonal Ribosomes

(2014) Christoph Lammers et al.   CHEMBIOCHEM

Enhancing the Efficiency and Regioselectivity of P450 Oxidation Catalysts by Unnatural Amino Acid Mutagenesis

(2014) Joshua N. Kolev et al.   CHEMBIOCHEM

Biological Applications of Expanded Genetic Codes

(2014) Xiang Li et al.   CHEMBIOCHEM

Cell Surface Display Yields Evolvable, Clickable Antibody Fragments

(2014) James A. Van Deventer et al.   CHEMBIOCHEM

A Bacterial Strain with a Unique Quadruplet Codon Specifying Non-native Amino Acids

(2014) Abhishek Chatterjee et al.   CHEMBIOCHEM

Engineering Transaminase for Stability Enhancement and Site-Specific Immobilization through Multiple Noncanonical Amino Acids Incorporation

(2014) Kanagavel Deepankumar et al.   ChemCatChem

Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins

(2014) Kathrin Lang et al.   CHEMICAL REVIEWS

Site-specific PEGylation of Proteins: Recent Developments

(2014) Nicole Nischan et al.   JOURNAL OF ORGANIC CHEMISTRY

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

(2014) Ying Ma et al.   MOLECULES

Site-Specific Bioconjugation of a Murine Dihydrofolate Reductase Enzyme by Copper(I)-Catalyzed Azide-Alkyne Cycloaddition with Retained Activity

(2014) Sung In Lim et al.   PLoS One

The “Gate Keeper” Role of Trp222 Determines the Enantiopreference of Diketoreductase toward 2-Chloro-1-Phenylethanone

(2014) Hairong Ma et al.   PLoS One

Genetic incorporation of unnatural amino acids biosynthesized from α-keto acids by an aminotransferase

(2014) Jae-Eun Jung et al.   Chemical Science

Selective chemical protein modification

(2014) Christopher D. Spicer et al.   Nature Communications

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-Chelating Unnatural Amino Acid

(2013) Xiaohong Liu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Controlling enzyme inhibition using an expanded set of genetically encoded amino acids

(2013) Shun Zheng et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein conjugation with genetically encoded unnatural amino acids

(2013) Chan Hyuk Kim et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein engineering with unnatural amino acids

(2013) William H Zhang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Biochemical analysis with the expanded genetic lexicon

(2012) Hui-wang Ai   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Structural basis for the enhanced stability of highly fluorinated proteins

(2012) B. C. Buer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ω-Transaminases for the Production of Optically Pure Amines and Unnatural Amino Acids

(2012) Sam Mathew et al.   ACS Catalysis

Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis

(2011) John T. Ngo et al.   ACCOUNTS OF CHEMICAL RESEARCH

Bioconjugation ofl-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

(2011) Niraikulam Ayyadurai et al.   BIOCONJUGATE CHEMISTRY

Manipulation of enzyme properties by noncanonical amino acid incorporation

(2011) Shun Zheng et al.   Biotechnology Journal

Enhanced Refoldability and Thermoactivity of Fluorinated Phosphotriesterase

(2011) Peter James Baker et al.   CHEMBIOCHEM

A facile and efficient method for the incorporation of multiple unnatural amino acids into a single protein

(2011) Niraikulam Ayyadurai et al.   CHEMICAL COMMUNICATIONS

Fluorinated amino acids: compatibility with native protein structures and effects on protein–protein interactions

(2011) Mario Salwiczek et al.   CHEMICAL SOCIETY REVIEWS

Modulating substrate specificity of histone acetyltransferase with unnatural amino acids

(2011) Kinjal Rajesh Mehta et al.   Molecular BioSystems

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

Engineering of Biocatalysts: from Evolution to Creation

(2011) Maureen B. Quin et al.   ACS Catalysis

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to “Teflon” Proteins

(2010) Lars Merkel et al.   CHEMBIOCHEM

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Replacing 32 Proline Residues by a Noncanonical Amino Acid Results in a Highly Active DNA Polymerase

(2010) Bastian Holzberger et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Residue-specific global fluorination of Candida antarctica lipase B in Pichia pastoris

(2010) Nediljko Budisa et al.   Molecular BioSystems

Mutagenesis and Evolution of Sulfated Antibodies Using an Expanded Genetic Code

(2009) Chang C. Liu et al.   BIOCHEMISTRY

Evolution of Proteins with Genetically Encoded “Chemical Warheads”

(2009) Chang C. Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanded chemical diversity sampling through whole protein evolution

(2009) Amy J. Baldwin et al.   Molecular BioSystems

Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code

(2009) Chang C Liu et al.   Nature Protocols

Unnatural Amino Acid Incorporation into Virus-Like Particles

(2008) Erica Strable et al.   BIOCONJUGATE CHEMISTRY

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast

(2008) Qian Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Azatryptophans endow proteins with intrinsic blue fluorescence

(2008) S. Lepthien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein evolution with an expanded genetic code

(2008) C. C. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA