期刊
BIOMED RESEARCH INTERNATIONAL
卷 2015, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2015/419549
关键词
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资金
- Division de Investigacion Bogota (DIB)-Vicerrectoria de Investigacion, Universidad Nacional de Colombia [16060, 14837, 14337]
- Academic Direction, Universidad Nacional de Colombia
- Jovenes Investigadores e Innovadores Program, Colciencias, Colombia
- Intramural Research Program of the National Institutes of Health, National Library of Medicine
- National Center for Biotechnology Information (NCBI)
- NATIONAL LIBRARY OF MEDICINE [ZIALM082713] Funding Source: NIH RePORTER
Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the present study, we demonstrated that M. colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed that M. colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M. avium. Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M. colombiense CECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.
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