期刊
ULTRASOUND IN MEDICINE AND BIOLOGY
卷 36, 期 9, 页码 1470-1480出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2010.06.010
关键词
Contrast agents; Gene delivery; Sonoporation; Ultrasound
资金
- National Institutes of Health NIBIB [EB002185]
- NHBLI [HL090700]
- American Heart Association
- Coulter Translational Research Grant
We investigated a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells in vitro under a range of acoustic pressures (0 to 950 kPa) and pulse durations (0 to 100 cycles). No effect on gene transfection or viability was observed from application of microbubbles, DNA or ultrasound alone. Microbubbles in combination with ultrasound (500-kPa, 1-MHz, 50-cycle bursts at a pulse repetition frequency [PRF] of 100 Hz) significantly reduced viability both with DNA (53 +/- 27%) and without (19 +/- 8%). Maximal gene transfection (similar to 1% of cells) occurred using 50-cycle, 1-MHz pulses at 300 kPa, which resulted in 40% viability of cells. We demonstrated that we can locally deliver DNA to vascular smooth muscle cells in vitro using microbubble carriers and focused ultrasound. (E-mail: jh7fj@virginia.edu) (C) 2010 World Federation for Ultrasound in Medicine & Biology.
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