Article
Biochemical Research Methods
Yang Liu, Bingxi Liu, John Green, Carly Duffy, Ming Song, James D. Lauderdale, Peter Kner
Summary: The combination of an electrically tunable lens and adaptive optics allows for high-resolution imaging of a volume of 499 x 499 x 192 cubic micrometers. Compared to a system without adaptive optics, the signal to background ratio has increased by a factor of 3.5. While the current imaging speed is 7s/volume, it should be easy to increase it to under 1s per volume.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Optics
Yue Wang, Jiarui Lei, Jianfeng Zheng, Xulongoi Wang, Miao Cheng, Ming Liu, Junan Zhang, Weibin Chen, Xiaoyao Hu, Weizhong Gu, Shiwei Guo, Xiaobo Hu, Zhigang Gao, Dong Liu
Summary: A microscope requires careful assembly, alignment, and testing due to its complex lenses. Chromatic aberration correction is important but can increase weight and cost. However, this paper proposes an algorithm that shifts some correction tasks to post-processing, achieving higher-quality images without changing hardware or optical parameters.
Article
Materials Science, Multidisciplinary
Zhichun Zhou, Binbin Jiang, Jianke Qiu, Linglei Zhang, Jiafeng Lei, Rui Yang, Kui Du
Summary: This study investigates the mechanism of crack formation in titanium alloys with lamellar microstructure under low-cycle dwell fatigue. The accumulation of residual dislocations and local tensile stress at the α/β interfaces are found to be the key factors leading to the formation of dwell fatigue cracks.
SCIENCE CHINA-MATERIALS
(2023)
Article
Physics, Multidisciplinary
Biwei Zhang, Jiazhu Zhu, Ke Si, Wei Gong
Summary: The proposed method utilizes deep learning neural networks to rapidly correct optical aberrations, achieving high degrees of freedom corrections while maintaining fast speed. Experimental results demonstrate good performance in correcting aberrations of different complexities, and the method has potential applications in deep tissue imaging and large volume imaging without the need for extra devices.
FRONTIERS IN PHYSICS
(2021)
Review
Materials Science, Multidisciplinary
Knut W. Urban, Juri Barthel, Lothar Houben, Chun-Lin Jia, Lei Jin, Markus Lentzen, Shao-Bo Mi, Andreas Thust, Karsten Tillmann
Summary: Transmission electron microscopy is a crucial tool for studying the structure and properties of materials at atomic resolution. It allows for high spatial resolution and enables the correlation between microstructure and macroscopic properties. With the development of aberration-corrected electron optics, it became possible to perform picometer-scale measurements and chemical analyses. This paper aims to introduce this new type of electron microscopy, discuss its implications, applications, and limitations in materials science, and provide access to relevant literature.
PROGRESS IN MATERIALS SCIENCE
(2023)
Article
Optics
Chenda Lu, Qinghua Tian, Lei Zhu, Ran Gao, Haipeng Yao, Feng Tian, Qi Zhang, Xiangjun Xin
Summary: In this work, an attention-based adaptive optics method is proposed, which integrates phase diversity with a convolutional neural network (CNN) using a non-local block. Simulation results demonstrate the effectiveness of the proposed method in mitigating the ambiguity problem of phase retrieval and its superior performance compared to traditional CNN-based wavefront correction.
Article
Biochemical Research Methods
Ruofei Wu, Jiaxiong Luo, Jiancong Li, Hanbao Chen, Junrui Zhen, Sicong Zhu, Zicong Luo, Yanxiong Wu
Summary: This study proposes an aberration correction method (AA-P algorithm) based on an improved phase recovery strategy, which optimizes the spectral function and optical pupil function updates of the samples using adaptive modulation factors added during the iterations. Experimental results show that the proposed AA-P algorithm can recover complex amplitude images with clearer contours and higher phase contrast in optical systems with hybrid aberrations. The algorithm improves the image reconstruction quality by 82.6% compared to the existing EPRY-FPM algorithm.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Microscopy
Shigeyuki Morishita, Hidetaka Sawada
Summary: Spherical aberration correctors using hexapole fields are widely used and are pivotal in atomic-resolution imaging. Two types of spherical aberration correctors, a four-hexapole corrector and a two-hexapole corrector, were proposed to compensate for higher-order geometrical aberrations.
Article
Optics
Sharika Mohanan, Lexander D. Corbett
Summary: It has been demonstrated that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane, and this study proposes a compensation method using a correction collar controlled by a stepper motor. The study verifies the accuracy of the compensation method using a Shack-Hartmann wavefront sensor, and discusses the limited impact of spherical aberration compensation on the diffraction limited range of the remote focusing system.
Article
Neurosciences
Stephane Bancelin, Luc Mercier, Emanuele Murana, U. Valentin Nagerl
Summary: The study aimed to develop and validate a method based on adaptive optics for a priori correction of spherical aberrations to improve the spatial resolution of STED microscopy inside thick biological tissue. Experimental results demonstrated that the corrections substantially increased image quality in living brain slices, allowing for clear measurement of small structures at depth.
Article
Materials Science, Multidisciplinary
Wei Yan, Yangrui Huang, Luwei Wang, Yong Guo, Jin Li, Yinru Zhu, Zhigang Yang, Junle Qu
Summary: An adaptive optics system based on genetic algorithm was introduced for aberration correction in two-photon fluorescence microscopy, resulting in improved signal intensity and imaging depth. The method was successfully applied to two-photon fluorescence lifetime imaging, leading to enhanced signal-to-noise ratio and accuracy in lifetime analysis. In addition, this technique can be extended to other experiments such as three-photon microscopy, light-sheet microscopy, and super-resolution microscopy.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Optics
B. Miao, L. Feder, J. E. Shrock, H. M. Milchberg
Summary: This article introduces a method to retrieve the complex amplitude of a Bessel beam from intensity measurements alone, and then correct the wavefront and intensity profile using a deformable mirror.
Article
Materials Science, Multidisciplinary
Chaoshuai Guan, Bin Chen, Liping Jiang, Abdul Karim, Lei Jia, Yang Hu, Yuping Liang, Xia Deng, Junwei Zhang, Liu Zhu, Kaiqi Bi, Hongli Li, Hong Zhang, Mingsu Si, Yong Peng
Summary: In this study, omega phase transformation in Galfenol under low temperature aging was observed, and it was found that the omega phase precipitates deteriorate the magnetostriction of Galfenol. This provides important insights into the structural evolution of Galfenol and its magnetostrictive performance.
MATERIALS & DESIGN
(2021)
Article
Optics
Xunbo Yu, Hanyu Li, Xinzhu Sang, Xiwen Su, Xin Gao, Boyang Liu, Duo Chen, Yuedi Wang, Binbin Yan
Summary: This study demonstrates a method of reducing lens aberrations and improving image quality in light-field displays using a pre-correction convolutional neural network. By transforming the elemental image array generated by a virtual camera array into a pre-corrected EIA, higher quality 3D images are achieved through optical transformation of the lens array. The proposed method is validated through simulations and optical experiments, resulting in a 70-degree viewing angle light field display with improved image quality.
Article
Microscopy
Ryo Ishikawa, Riku Tanaka, Shigeyuki Morishita, Yuji Kohno, Hidetaka Sawada, Takuya Sasaki, Masanari Ichikawa, Masashi Hasegawa, Naoya Shibata, Yuichi Ikuhara
Summary: Recent advancements in aberration correction technology have significantly improved the illumination angle in scanning transmission electron microscopy (STEM), allowing for larger depth resolution. However, minimizing low-order aberrations is crucial for three-dimensional depth sectioning with large illumination angles.
Article
Microscopy
C. Gao, C. Hofer, T. J. Pennycook
Summary: Ptychography provides high dose efficiency images that can reveal light elements next to heavy atoms. However, contrast reversals can occur when the projected potential becomes strong. Recent research has shown that these reversals can be counteracted by adapting the focus. This study explains why the best contrast is often found with the probe focused to the middle of the sample and highlights the importance of convergence angle in thin samples to remove contrast reversals.
Article
Microscopy
J. Lindner, U. Ross, T. Meyer, V. Boureau, M. Seibt, Ch. Jooss
Summary: Phase-shifting electron holography is an excellent method with high phase sensitivity to reveal electron wave phase information. An advanced drift correction scheme is proposed in this study, which exploits the interface of the TEM specimen and the vacuum area in the hologram. This method allows for obtaining reliable phase information.
Article
Microscopy
Ali Jaberi, Nicolas Brodusch, Jun Song, Raynald Gauvin
Summary: This study investigates knock-on damage in lithium-ion batteries (LIBs) by computing threshold displacement energies (TDEs) and performing Monte Carlo simulation. The results indicate that Li is most sensitive to knock-on damage at moderate electron energies, and TDE is the principal parameter for assessing Li sensitivity to knock-on damage across similar structures.
Article
Microscopy
Alexander Schroeder, Christopher Rathje, Leon van Velzen, Maurits Kelder, Sascha Schaefer
Summary: This study utilizes novel event-based electron detector platforms to extend the temporal resolution of electron microscopy. By training a neural network to predict electron arrival time, the researchers were able to improve the timing accuracy and achieve a promising solution for enhancing electron timing precision in various electron microscopy applications.
Article
Microscopy
Avi Auslender, Nivedita Pandey, Amit Kohn, Oswaldo Dieguez
Summary: This article describes a faster implementation based on DFT for computing the mean inner potential of crystals, providing quantum-mechanical calculations beyond the independent-atom approximation. The study also reveals the dependence of the mean inner potential on sample boundary conditions, mass density, and magnetic susceptibility, highlighting its correlation with various material properties.
Article
Microscopy
Zhidong Yang, Dawei Zang, Hongjia Li, Zhao Zhang, Fa Zhang, Renmin Han
Summary: In this work, we propose a self-supervised deep learning model for cryo-ET volumetric image denoising based on noise modeling and sparsity guidance. Experimental results demonstrate that our method can achieve reliable denoising by training on single noisy volume and outperform existing methods.
Article
Microscopy
J. Kuttruff, J. Holder, Y. Meng, P. Baum
Summary: In this study, a robust clustering algorithm is proposed that can find clusters in a continuous stream of raw data in real time. This algorithm converts pixel hits measured by hybrid pixel detectors to real single-electron events. By continuously comparing with previous hits, the algorithm efficiently identifies the merging of new and old events.
Article
Microscopy
D. G. Senturk, C. P. Yu, A. De Backer, S. Van Aert
Summary: This article presents a statistics-based method for accurately counting the number of atoms in nanostructures, especially for images acquired with low electron doses and multiple element structures.
Article
Microscopy
Mauricio J. Prieto, Lucas de Souza Caldas, Liviu C. Tanase, Thomas Schmidt, Oscar Rodriguez de la Fuente
Summary: This study presents a synchrotron-based investigation of the synthesis process of a magnetite/hematite bilayer. Ion bombardment gradually transforms hematite into magnetite, and the growth of magnetite leads to the formation of stable boundaries. These findings are significant for understanding novel oxide heterostructures.
Article
Microscopy
Emre Yoruk, Holger Klein, Stephanie Kodjikian
Summary: Beam sensitive nanomaterials pose challenges for crystallographic structure determination. A dose symmetric electron diffraction tomography (DS-EDT) method is developed to reduce beam damage and obtain complete data sets by merging individual datasets from multiple crystals.
Article
Microscopy
A. Pofelski, Y. Zhu, G. A. Botton
Summary: The precision and sensitivity of the GPA method for strain characterization is a widely discussed topic. This study introduces the concept of phase noise and analyzes the parameter of sampling to improve the precision of GPA. Experimental and theoretical results demonstrate that using a larger pixel spacing in STEM can enhance the precision and sensitivity of the GPA method.
Article
Microscopy
Sangjun Kang, Di Wang, Christian Kuebel, Xiaoke Mu
Summary: Transmission electron microscopy is a valuable tool for assessing strain fields within materials. However, using thin specimens in TEM analysis can affect atomic configuration and deformation structure.