Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Haichuan Wang, Yi Zhou, Hongwei Xu, Xue Wang, Yi Zhang, Runze Shang, Marie O'Farrell, Stephanie Roessler, Carsten Sticht, Andreas Stahl, Matthias Evert, Diego F. Calvisi, Yong Zeng, Xin Chen
Summary: This study evaluated the therapeutic efficacy of the FASN inhibitor TVB3664 for HCC treatment and found that it has limited effectiveness as monotherapy, but can be combined with other drugs for improved results. These combination therapies can be developed based on driver oncogenes, supporting precision medicine approaches for HCC treatment.
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Cell Biology
Qiqiao Du, Pan Liu, Chunyu Zhang, Tianyu Liu, Wei Wang, Chunliang Shang, Jieyu Wu, Yuandong Liao, Yili Chen, Jiaming Huang, Hao Tan, Yunhe Zhao, Meng Xia, Junxiu Liu, Shuzhong Yao
Summary: This study uncovers a novel molecular mechanism in lymph node metastasis (LNM) of cervical cancer (CC) and identifies fatty acid synthase (FASN) as a crucial player and a potential therapeutic target for LNM in CC.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Oncology
Anna Han, Dzmitry Mukha, Vivian Chua, Timothy J. Purwin, Manoela Tiago, Bhavik Modasia, Usman Baqai, Jenna L. Aumiller, Nelisa Bechtel, Emily Hunter, Meggie Danielson, Mizue Terai, Philip B. Wedegaertner, Takami Sato, Solange Landreville, Michael A. Davies, Stefan Kurtenbach, J. William Harbour, Zachary T. Schug, Andrew E. Aplin
Summary: Metastatic uveal melanoma is difficult to treat, but targeting metabolic pathways, such as lipid/fat metabolism and mTOR, may be a potential therapeutic approach. The upregulation of enzymes involved in lipid/fat metabolism, such as FASN, and activation of the mTOR pathway were observed in uveal melanoma cells. Inhibiting FASN and mTOR led to the suppression of uveal melanoma cell growth, suggesting a novel treatment strategy for uveal melanoma.
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Biochemistry & Molecular Biology
Wenxia Wei, Baifu Qin, Weijie Wen, Boyu Zhang, Haidan Luo, Yuzhi Wang, Hui Xu, Xiaoshan Xie, Sicheng Liu, Xin Jiang, Mengan Wang, Qin Tang, Jiayu Zhang, Runxiang Yang, Zongmin Fan, Haiwen Lyu, Junzhong Lin, Kai Li, Mong-Hong Lee
Summary: Continuous de novo fatty acid synthesis is essential for tumor growth. FBXW7 beta, an isoform of FBXW7 highly mutated in colorectal cancer (CRC), functions as an E3 ligase for fatty acid synthase (FASN). Mutations in FBXW7 beta, specific to CRC, result in sustained lipogenesis. The oncogenic marker CSN6 interacts with and stabilizes FASN, promoting lipogenesis and poor prognosis in CRC. The CSN6-FASN axis, regulated by EGF, contributes to tumor growth and can be targeted with combination therapy of orlistat and cetuximab for CSN6/FASN-high CRC.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Multidisciplinary Sciences
Marie O'Farrell, Greg Duke, Richard Crowley, Douglas Buckley, Eduardo B. Martins, Dipankar Bhattacharya, Scott L. Friedman, George Kemble
Summary: FASN inhibition can reduce the development of fatty liver and fibrosis, and alleviate the inflammatory and fibrotic drivers of NASH by inhibiting immune and stellate cell activity.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Hinata Maeno, Satoshi Ogata, Tetsuhide Shimizu, Ming Yang
Summary: The authors have developed a film-stacked structure using polyethylene terephthalate (PET) films, which can be combined with 96-well microplates for biochemical analysis. By inserting this structure into a well and rotating it, convection flow is generated to enhance chemical reactions between molecules. However, only a part of the solution circulates into the narrow gaps between the films, resulting in lower reaction efficiency. In this study, unsteady rotation is applied to improve analyte transport into the gaps using secondary flow generated on the rotating disk surface. Finite element analysis is used to optimize the rotation conditions and evaluate the molecular binding ratio.
Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
