Article
Chemistry, Medicinal
Molly Congdon, Russell G. Fritzemeier, Yugesh Kharel, Anne M. Brown, Vlad Serbulea, David R. Bevan, Kevin R. Lynch, Webster L. Santos
Summary: Elevated levels of Sphingosine 1-phosphate (S1P) and increased expression of Sphingosine kinase iso-forms (SphK1 and SphK2) are associated with various disease states. The development of selective inhibitors for SphK1 and SphK2 has become a focus of drug discovery, with studies focusing on optimizing binding in the SphK2 substrate binding site. The identification of indole-based compounds with 1,5-disubstitution as potent inhibitors highlights the potential for targeting SphK2 with improved potency and selectivity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Lan Dai, Chen Wang, Wenjing Wang, Keqi Song, Taiyang Ye, Jie Zhu, Wen Di
Summary: This study reveals the involvement of adipocytes in promoting the growth of epithelial ovarian cancer (EOC) and identifies sphingosine kinase 2 (SphK2) as a key mediator in this process. Inhibition of SphK2 significantly suppresses adipocyte-induced EOC cell proliferation, which is dependent on the activation of extracellular signal-regulated protein kinases (ERK). Furthermore, silencing SphK2 inhibits the adipocyte-induced expression of phospho-ERK and c-Myc, two crucial factors in EOC growth.
Article
Multidisciplinary Sciences
Khalil Khashei Varnamkhasti, Mehdi Moghanibashi, Sirous Naeimi
Summary: Lymph node metastasis is a crucial factor in the prognosis of lung squamous cell carcinoma. This study aimed to identify potential prognostic biomarkers shared between primary tumors and lymphatic metastasis. A total of 397 differentially expressed genes were identified, and 56 of them showed significant correlation with reference genes, highlighting their potential for identifying lymph node metastasis in lung squamous cell carcinoma.
SCIENTIFIC REPORTS
(2023)
Review
Oncology
Rui Liu, Kunjun Sun, Yuanda Wang, Yunxian Jiang, Jianyong Kang, Hong Ma
Summary: The study found that high PCNA expression is more prevalent in OSCC than low PCNA expression, while there is no significant difference between p53 and OSCC. Low PCNA expression is associated with higher 5-year overall survival in OSCC patients compared to high PCNA expression, and p53 negative patients have a higher 5-year overall survival than p53 positive patients.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Shan Lu, Zhongyun Dong
Summary: This study demonstrates that PCNA interacts with AR and its constitutively active variants, and the inhibition of PCNA-AR interaction can suppress AR transcriptional activity and the expression of AR target genes. Targeting the PCNA-AR interaction may represent an innovative and selective therapeutic strategy against prostate cancer, especially castration-resistant prostate cancers overexpressing constitutively active AR-Vs.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Fisheries
Simiao Yu, Xue Qiao, Xiaorui Song, Ying Yang, Dan Zhang, Wending Sun, Lingling Wang, Linsheng Song
Summary: In this study, a crucial replication accessory factor CgPCNA was identified from oysters and found to be highly expressed in agranulocytes and potential hematopoietic sites. The expression of CgPCNA was up-regulated after stimulation with LPS and Vibrio splendidus. These findings suggest that CgPCNA may serve as a marker for hemocyte proliferation in oysters.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Cell Biology
Vanessa Villegas-Ruiz, Antonio Romo-Mancillas, Isabel Medina-Vera, Kattia Alejandra Castro-Lopez, Josselene Carina Ramirez-Chiquito, Marco Antonio Fonseca-Montano, Mercedes Edna Garcia-Cruz, Roberto Rivera-Luna, Julieta Griselda Mendoza-Torreblanca, Sergio Juarez-Mendez
Summary: Leukemia is a common childhood malignancy in Mexico with low treatment success rate. The study found that there are multiple transcript variants of the proliferating cell nuclear antigen (PCNA) gene, and some of these variants are expressed at significantly low levels in acute lymphoblastic leukemia patients, which could serve as potential molecular markers for relapse.
Article
Multidisciplinary Sciences
David Martin-Hernandez, Irene L. Gutierrez, Marta Gonzalez-Prieto, Karina S. MacDowell, Javier Robledo-Montana, Hiram Tendilla-Beltran, Natalia Calleja-Rodriguez, Alvaro G. Bris, Cristina Ulecia-Moron, Beatriz Moreno, Javier R. Caso, Borja Garcia-Bueno, Sandra Rodrigues-Mascarenhas, Ignacio Marin-Jimenez, Juan Carlos Leza, Luis Menchen
Summary: The chronic inflammatory process in inflammatory bowel diseases (IBD) is mainly driven by T-cell response to microbial and environmental antigens. Psychological stress can trigger clinical flares of IBD, and sphingosine-1-phosphate (S1P) is involved in T-cell recruitment. By evaluating the impact of stress and the absence of sphingosine kinase 2 (Sphk2) on the colon of mice, it was found that stress increased S1P levels in the colon, possibly due to decreased degradation enzymes and Sphk2. This S1P accumulation could lead to inflammation and immune dysregulation, contributing to the development of IBD.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Yuanbao Zhou, Shishuo Zhang, Biaoxiong Wang, Ying Ran, Shaoyu Tu, Zhipeng Lin, Meilin Jin, Huanchun Chen, Jiahui Zou, Hongbo Zhou
Summary: A study found that the genome of Influenza A virus (IAV) transcribes and replicates in the nucleus, and the viral ribonucleoprotein (vRNP) complex plays a crucial role in viral replication. The polymerase basic protein 2 (PB2) is a major component of the vRNP complex and is translocated to the nucleus through its nuclear localization signals mediated by importins. However, it was discovered that proliferating cell nuclear antigen (PCNA) inhibits the nuclear import of PB2 and subsequently impairs viral replication. PCNA interacts with PB2 and inhibits its nuclear import, decreases the binding efficiency of PB2 with importin alpha, and affects vRNP assembly and polymerase activity. Overall, these findings highlight the negative regulatory role of PCNA in virus replication.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Medicine, General & Internal
Ani C. S. Simila, T. Isaac Joseph, K. L. Girish, T. Prasanth, Angelin Binu, Jeslin Mary
Summary: Expressional comparison and correlation of CD57 and PCNA in different grades of oral squamous cell carcinoma (OSCC) were conducted by immunohistochemistry. The results showed a significant decrease in CD57 labeling index from well-differentiated to poorly differentiated squamous cell carcinoma, whereas a significant increase in PCNA labeling index was observed. The combination of CD57 and PCNA biomarkers appears to be good indicators of the immune status of the patient and the aggressiveness of the lesion.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Chemistry, Medicinal
Aimee J. Horsfall, Theresa Chav, Jordan L. Pederick, Zoya Kikhtyak, Bethiney C. Vandborg, Wioleta Kowalczyk, Denis B. Scanlon, Wayne D. Tilley, Theresa E. Hickey, Andrew D. Abell, John B. Bruning
Summary: A modular peptidomimetic was designed in this study to interact with PCNA and achieve cellular and nuclear permeability, showing promising potential in inhibiting cancer cell proliferation. The peptidomimetic consisted of a p21-derived peptide as the starting scaffold, with a fluorescein tag and SV40 nuclear localization signal attached. The attachment of the fluorescein tag directly influenced the cellular uptake of the peptidomimetic, with fluorescein being essential for nuclear permeability. This work represents an important advancement in the development of PCNA targeting peptidomimetics for anti-cancer agents or cancer diagnostics.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Xiaowei Liu, Yuanxia Cai, Cheng Cheng, Yaoyao Gu, Xiaoxiao Hu, Kai Chen, Yeming Wu, Zhixiang Wu
Summary: PCLAF is overexpressed in neuroblastoma and is associated with poor prognosis. Knocking down PCLAF restricts neuroblastoma cell proliferation and affects cell cycle-related pathways. PCLAF accelerates the G1/S transition of neuroblastoma cell cycle by activating the E2F1/PTTG1 signaling pathway.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Masoumeh Moradi-Ozarlou, Sana Moshari, Hamed Rezaei Agdam, Amir Nomanzadeh, Simineh Shahmohamadlou, Mazdak Razi
Summary: The study found that obese rats induced by HFD showed a significant increase in mRNA levels of Hsp70-2a and Hsp90, while the mRNA levels of PCNA and the percentage of PCNA+ cells were decreased in the obese group. Additionally, obesity led to a significant decrease in testicular TAC, resulting in a reduction in serum testosterone levels, along with cell DNA and RNA damage.
Review
Biochemistry & Molecular Biology
Rocio Diaz Escarcega, Louise D. McCullough, Andrey S. Tsvetkov
Summary: S1P, a bioactive lipid molecule synthesized by SPHK1 and SPHK2, plays key roles in various signaling pathways, with SPHK2 regulating processes in the nucleus and mitochondria. Investigation into SPHK2 as a target for age-associated conditions is ongoing.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Food Science & Technology
Quanwei Zhang, Libo Huang, Bo Leng, Yang Li, Ning Jiao, Shuzhen Jiang, Weiren Yang, Xuejun Yuan
Summary: The study revealed that Zearalenone (ZEA) has adverse effects on the morphology, expression, and distribution of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestines of weaned gilts. However, the small intestine can resist or alleviate these adverse effects of ZEA by modulating the autocrine of ghrelin in intestinal epithelial cells.
Article
Nutrition & Dietetics
Peng Liu, Xiaohui Su, Mu Li, Hongmei Shen, Jun Yu, Patrick J. Kelly, Fangang Meng, Lixiang Liu, Lijun Fan, Ming Li, Shoujun Liu, Dianjun Sun
BRITISH JOURNAL OF NUTRITION
(2016)
Article
Endocrinology & Metabolism
Ming Li, Haiming Sun, Shoujun Liu, Jun Yu, Qiang Li, Peng Liu, Hongmei Shen, Dianjun Sun
Article
Cell Biology
Qiu-shi Wang, Ming Li, Lin-you Zhang, Yan Jin, Dan-dan Tong, Yang Yu, Jing Bai, Qi Huang, Fang-Li Liu, An Liu, Ki-Young Lee, Song-bin Fu
Article
Oncology
Ming Li, Yan Jin, Wen-jing Sun, Yang Yu, Jing Bai, Dan-dan Tong, Ji-ping Qi, Jin-rong Du, Jing-shu Geng, Qi Huang, Xiao-yi Huang, Yun Huang, Fei-fei Han, Xiang-ning Meng, Jesusa L. Rosales, Ki-Young Lee, Song-bin Fu
JOURNAL OF PATHOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Jing Bai, Changlong Guo, Wenjing Sun, Ming Li, Xiangning Meng, Yang Yu, Yan Jin, Dandan Tong, Jingshu Geng, Qi Huang, Jiping Qi, Songbin Fu
MOLECULAR BIOLOGY REPORTS
(2012)
Article
Cell Biology
Dan-dan Tong, Ying Jiang, Ming Li, Dan Kong, Xiang-nin Meng, Yu-zhen Zhao, Yan Jin, Jing Bai, Song-bin Fu, Jing-shu Geng
Article
Oncology
Yu Liu, Yanan Liang, Ming Li, Duanyang Liu, Jing Tang, Weiwei Yang, Dandan Tong, Xiaoming Jin
Article
Nutrition & Dietetics
Lanchun Liu, Lixiang Liu, Ming Li, Yang Du, Peng Liu, Lijun Fan, Fangang Meng
Summary: The policy of Universal Salt Iodisation (USI) helps reduce thyroid volume, but may mask the impact of height and weight on TVOL. Factors like age, weight, and salt iodine significantly influence TVOL.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Nutrition & Dietetics
Shuli An, Lijun Fan, Ming Li, Zhengyuan Wang, Shoujun Liu, Yutong Ji, Lixiang Liu, Yang Du, Fangang Meng, Peng Liu
Summary: In areas with high water iodine concentration, both water iodine and urinary iodine levels influence thyroid volume and goiter rate in children, while non-iodised salt coverage rate only affects the goiter rate. When the water iodine concentration exceeds 420 μg/l or urinary iodine concentration surpasses 800 μg/l, the goiter rate increases rapidly. Maintaining a non-iodised salt coverage rate of over 85% can help reduce the risk of goiter in children in high water iodine areas.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Baozhu Yi, Shuaibin Wang, Xinran Wang, Zhichao Liu, Chao Zhang, Ming Li, Song Gao, Shi Wei, Sejong Bae, Erica Stringer-Reasor, Lizhong Wang, Runhua Liu
Summary: MiR-3662 is minimally expressed in normal tissues but highly expressed in various cancers, especially in TNBC and African American breast cancer. Inhibition or knockout of miR-3662 suppresses proliferation and migration of TNBC cells by reducing Wnt/β-catenin signaling activation. HMG-box transcription factor 1 (HBP-1) is identified as a target of miR-3662 responsible for TNBC cell proliferation.
Article
Endocrinology & Metabolism
Guan-ying Nie, Rui Wang, Peng Liu, Ming Li, Dian-jun Sun
Summary: This study found that mild anemia may affect thyroid function in pregnant women during the first trimester, highlighting the importance of excluding mild anemia confounding when establishing a locally derived specific reference interval for early pregnancy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
He Qian, Su Xiao Hui, Liu Peng, Fan Li Jun, Meng Fan Gang, Liu Li Xiang, Li Ming, Liu Shou Jun
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2018)