microR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages

MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-kappa B (NF-kappa B1), TNF-alpha and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down- regulating IRAK-1 protein expression. (C) 2013 Elsevier Ltd. All rights reserved.