Article
Computer Science, Information Systems
K. S. Mithra, W. R. Sam Emmanuel
Summary: This study introduces a method for diagnosing tuberculosis using the Gaussian mixture model and fuzzy neural network combination of GFNN technology. Experimental results demonstrate the advantages of this technique in terms of diagnostic accuracy.
JOURNAL OF KING SAUD UNIVERSITY-COMPUTER AND INFORMATION SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Conghao Wang, Qinmiao Chen, Huilan Liu, Runlong Wu, Xiong Jiang, Qiang Fu, Zhe Zhao, Ye Zhao, Yuqian Gao, Bosong Yu, Hongchen Jiao, Aimin Wang, Shumin Xiao, Lishuang Feng
Summary: Miniature two-photon microscopy is a powerful technique for investigating brain activity in freely moving animals. This study successfully addressed the challenges of probe weight and animal behavior constraints by employing metalenses, and demonstrated two-photon imaging of mouse brain structures.
Article
Engineering, Electrical & Electronic
Panchun Gu, Zengxin Huang, Mi Ping, Wenshuang Li, Meng Xiang, Xizeng Feng, Dengfeng Kuang
Summary: This study presents a novel method to generate multiplane parallel light sheets with high resolution and low phototoxicity using an isosceles triangular array microstructure. Experimental validation and comparison with simulations were performed to evaluate the imaging quality, showing promising results for volumetric imaging in light sheet fluorescence microscopy.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Chemistry, Analytical
Hiroshi Aoki
Summary: A novel technique for microscopic observation of single microplastic particles stained with fluorescent dyes has been developed, enabling fluorescence-based determination of the materials of these particles as a first screening of material determination. Commercially available microplastic particles and fluorescent dyes were used to observe the degree of fluorescent intensity for various combinations, revealing statistically significant differences in fluorescent intensity for different materials.
Article
Biochemistry & Molecular Biology
Jiamin Wu, Zhi Lu, Dong Jiang, Yuduo Guo, Hui Qiao, Yi Zhang, Tianyi Zhu, Yeyi Cai, Xu Zhang, Karl Zhanghao, Hao Xie, Tao Yan, Guoxun Zhang, Xiaoxu Li, Zheng Jiang, Xing Lin, Lu Fang, Bing Zhou, Peng Xi, Jingtao Fan, Li Yu, Qionghai Dai
Summary: The study introduces a computational imaging framework called DAOSLIMIT for high-speed, high-resolution 3D imaging, tiled wavefront correction, and low phototoxicity with a compact system. The research demonstrates the capabilities of observing large-scale cell migration and neural activities in mammals, as well as various subcellular dynamics during neutrophil migration and tumor cell circulation using this method.
Article
Infectious Diseases
Divya Anthwal, Rakesh Kumar Gupta, Narayan Sivaramakrishnan Gomathi, Srikanth Prasad Tripathy, Dasarathi Das, Sanghamitra Pati, Nikita Panwalkar, Prabha Desikan, Kiran Bala, Urvashi B. Singh, Manpreet Bhalla, Ritu Singhal, Ajoy Kumar Verma, Khalid Umar Khayyam, Vithal Prasad Myneedu, Rohit Sarin, Sandeep Sharma, Avi Kumar Bansal, Umesh D. Gupta, Sripad A. Patil, Abhinav Goyal, Ashawant Gupta, Manjula Singh, Nalini Kant Gupta, Sagarika Haldar, Jaya Sivaswami Tyagi
Summary: The study evaluated the performance of the 'TBDetect' kit-based bio-safe fluorescent microscopy filter in comparison to direct smear microscopy and culture for the detection of pulmonary tuberculosis in India. TBDetect showed a higher positivity rate compared to other methods, with efficient sputum concentration and disinfection confirmed through a six-site validation study. Scientists and technicians praised the ease of use and convenience of TBDetect microscopy during the study.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Biochemistry & Molecular Biology
Misako Nishigami, Yuki Uno, Kanta Tsumoto
Summary: In this study, the activation of GP64 and membrane fusion on giant liposomes were triggered using NPE-caged-proton. It was found that BVs prefer phosphatidylserine as they accumulated around GUVs with DOPS before the fusion reaction. This research provides valuable insights into the behavior of viruses under different chemical and biochemical environments.
Article
Biochemical Research Methods
Guangxing Wu, Serene Wen Ling Ng, Yan Zhou, Minghui Hong
Summary: Optical microscopic techniques are crucial for observing and extracting dynamic information from micro/nano-scale samples in microfluidic devices in biology and chemistry fields. However, current microfluidic imaging schemes face challenges in achieving high spatial and temporal resolutions simultaneously. The emergence of microsphere nanoscope offers a potential solution to address these challenges, with its high spatial resolution, real-time imaging abilities, and cost-effectiveness. In this study, a microsphere compound lens integrated microfluidic imaging device is proposed for real-time super-resolution imaging. The device exhibits superiority over single microsphere and conventional optical microscopes in discerning nano-objects beyond the diffraction limit and generating magnified images. Additionally, the device is experimentally demonstrated to be applicable in nanoparticle tracing and live-cell monitoring.
Review
Physiology
Kenneth W. Dunn, Bruce A. Molitoris, Pierre C. Dagher
Summary: The Indiana O'Brien Center is a research center funded by the NIH, dedicated to developing and disseminating advanced optical microscopy methods to support renal researchers worldwide. The center provides advanced imaging capabilities through collaborations and a unique fellowship program to renal researchers globally.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Yu-Jui Fan, Han-Yun Hsieh, Sheng-Fang Tsai, Cheng-Hsuan Wu, Chia-Ming Lee, Yen-Ting Liu, Chieh-Han Lu, Shu-Wei Chang, Bi-Chang Chen
Summary: The study developed a continuous cell-imaging system with subcellular resolution by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). By using a microfluidic channel sealed with a water refractive index-matched thin film, aberrations of the lightsheet entering the device were reduced, leading to clearer cell images.
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Article
Microbiology
Sidwell Mvo, Carine Bokop, Benjamin Longo-Mbenza, Sandeep D. Vasaikar, Teke Apalata
Summary: This study aimed to determine the factors associated with prolonged smear positivity in patients with multidrug-resistant tuberculosis (MDR-TB). The results showed that age, CD4+ T-cell count, and baseline smear grade were the key factors affecting smear positivity.
Article
Plant Sciences
Yosuke Toda, Toshiaki Tameshige, Masakazu Tomiyama, Toshinori Kinoshita, Kentaro K. Shimizu
Summary: Recent technical advances in computer vision have led to the development of methods for image-based quantification of stomata traits. A new platform for real-time stomata detection using machine learning and common hardware components is presented, offering a low-cost solution for phenotyping. The platform allows for comprehensive stomatal phenotype collection and accelerates research in stomata phenotyping.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Physical
Wenlong Yao, Qin Ling, Qing Dai, Shuyan Fang, Cheng Yang, Like Huang, Xiaohui Liu, Houcheng Zhang, Jing Zhang, Yuejin Zhu, Ziyang Hu
Summary: This paper systematically examines the degradation mechanisms of all-inorganic perovskite CsPbI2Br under a humid environment. It is found that the level of relative humidity (RH) is important for the degradation process and it initiates at the grain boundaries and extends toward the grain interiors.
ACS APPLIED ENERGY MATERIALS
(2022)
Article
Multidisciplinary Sciences
Wen Rongrong, Li Ru, He Sixiao, Wang Ziqing, Huang Junhao, Zhao Liying, Tian Zhihui, Ma Qiang
Summary: The study involves observing the intravascular dynamics of mouse liver tissue by injecting a dye to stain blood vessels, allowing for in-depth studies on tissue-related diseases. Multiphoton microscopy is utilized to capture high-resolution images of biological tissues in vivo, providing insights into cell and cell function in deep tissue sections or organs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Biochemical Research Methods
Catherine E. Majors, Chelsey A. Smith, Mary E. Natoli, Kathryn A. Kundrod, Rebecca Richards-Kortum
Article
Multidisciplinary Sciences
Pelham A. Keahey, Mathieu L. Simeral, Kristofer J. Schroder, Meaghan M. Bond, Prince J. Mtenthaonnga, Robert H. Miros, Queen Dube, Rebecca R. Richards-Kortum
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Otorhinolaryngology
Sarah M. Kidwai, Arjun K. Parasher, Victor J. Schorn, Elizabeth G. Demicco, Rebecca Richards-Kortum, Alfred Marc Iloreta, Satish Govindaraj, Brett A. Miles
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2018)
Article
Biochemical Research Methods
Mary E. Natoli, Brittany A. Rohrman, Carolina De Santiago, Gert U. van Zyle, Rebecca R. Richards-Kortum
ANALYTICAL BIOCHEMISTRY
(2018)
Article
Dentistry, Oral Surgery & Medicine
Eric C. Yang, Melody T. Tan, Richard A. Schwarz, Rebecca R. Richards-Kortum, Ann M. Gillenwater, Nadarajah Vigneswaran
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
(2018)
Article
Multidisciplinary Sciences
Megan Heenan, Jose D. Rojas, Z. Maria Oden, Rebecca Richards-Kortum
Article
Chemistry, Analytical
Mary E. Natoli, Megan M. Chang, Kathryn A. Kundrod, Jackson B. Coole, Gladstone E. Airewele, Venee N. Tubman, Rebecca R. Richards-Kortum
Summary: A rapid and low-cost nucleic acid test for detecting the point mutation in SCD has been developed in this study, showing high sensitivity and specificity with a turnaround time of less than 30 minutes. The test can be performed near the point of care and may be adapted for detecting other disease-causing point mutations in genomic DNA.
ANALYTICAL CHEMISTRY
(2021)
Editorial Material
Chemistry, Multidisciplinary
Emilie Newsham, Rebecca Richards-Kortum
ACS CENTRAL SCIENCE
(2021)
Article
Engineering, Biomedical
Catherine E. Majors, Michal E. Pawlowski, Tomasz S. Tkaczyk, Queen Dube, Rebecca Richards-Kortum
Summary: The study demonstrates that LeukoScope can assist in measuring WBC counts in pediatric patients but faces challenges in accurately assessing WBC counts in neonatal patients.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Pediatrics
Jennifer Carns, Sara Liaghati-Mobarhan, Aba Asibon, Alfred Chalira, Norman Lufesi, Elizabeth Molyneux, Maria Z. Oden, Rebecca Richards-Kortum, Kondwani Kawaza
Summary: The study revealed a significant improvement in survival rates for neonates weighing 1.0-1.3 kg after the implementation of CPAP in Malawi government hospitals, with the improvement sustained during the follow-up period. This demonstrates the effectiveness of a nurse-led CPAP program in improving outcomes for this neonatal population.
ARCHIVES OF DISEASE IN CHILDHOOD
(2022)
Article
Engineering, Biomedical
Jesse K. Adams, Dong Yan, Jimin Wu, Vivek Boominathan, Sibo Gao, Alex Rodriguez, Soonyoung Kim, Jennifer Carns, Rebecca Richards-Kortum, Caleb Kemere, Ashok Veeraraghavan, Jacob T. Robinson
Summary: Lensless microscopy with a designed phase mask allows for imaging of biological tissue in vivo, overcoming the limitations of existing techniques. The low-cost and small form factor of this lensless imaging method makes it suitable for clinical use in inaccessible areas of the body.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Engineering, Biomedical
Huayu Hou, Ruchika Mitbander, Yubo Tang, Ahad Azimuddin, Jennifer Carns, Richard A. Schwarz, Rebecca R. Richards-Kortum
Summary: This article reviews the recent development and application of low-cost optical imaging technologies, emphasizing the integration of artificial intelligence to improve in vivo cancer detection accuracy in low-resource settings. The article also discusses the challenges and future prospects of applying optical imaging technologies in clinical practice.
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2023)
Article
Pediatrics
Sonia E. Sosa Saenz, Mary Kate Hardy, Megan Heenan, Z. Maria Oden, Rebecca Richards-Kortum, Queen Dube, Kondwani Kawaza
BMJ PAEDIATRICS OPEN
(2020)
Article
Chemistry, Multidisciplinary
Catherine E. Majors, Michal E. Pawlowski, Daniel C. Burke, Tomasz S. Tkaczyk, Alyssa Rieber, Rebecca Richards-Kortum
Article
Engineering, Biomedical
Sonia Parra, Eduardo Carranza, Jackson Coole, Brady Hunt, Chelsey Smith, Pelham Keahey, Mauricio Maza, Kathleen Schmeler, Rebecca Richards-Kortum
IEEE JOURNAL OF TRANSLATIONAL ENGINEERING IN HEALTH AND MEDICINE
(2020)
