Article
Infectious Diseases
Jing Tong, Mengqiu Gao, Yu Chen, Jie Wang
Summary: This case report describes a 14-year-old girl with miliary pulmonary tuberculosis and extensively drug-resistant tuberculous meningitis (TBM). The final diagnosis was pre-extensive drug-resistant TBM. After individualized antituberculosis treatment, the patient recovered with no significant neurological sequelae.
BMC INFECTIOUS DISEASES
(2023)
Article
Immunology
Pinidphon Prombutara, Tegar Adriansyah Putra Siregar, Thanida Laopanupong, Phongthon Kanjanasirirat, Tanawadee Khumpanied, Suparerk Borwornpinyo, Awantika Rai, Angkana Chaiprasert, Prasit Palittapongarnpim, Marisa Ponpuak
Summary: The upsurge of multidrug-resistant infections has made tuberculosis the leading cause of death among infectious diseases. A clonal outbreak strain called MKR superspreader has been identified, which exhibits a faster growth rate and unique host gene expression pattern. IL-36RN may serve as a new target against this emerging multidrug-resistant M. tuberculosis strain.
Review
Clinical Neurology
Kenneth Ssebambulidde, Jane Gakuru, Jayne Ellis, Fiona V. Cresswell, Nathan C. Bahr
Summary: Diagnosis of TBM remains challenging due to the limitations of current diagnostic methods. A combination of clinical factors and various investigations are utilized for diagnosis. Starting empiric therapy promptly is crucial for good outcomes in many cases.
FRONTIERS IN NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Su-Young Kim, Dae Hun Kim, Seong Mi Moon, Ju Yeun Song, Hee Jae Huh, Nam Yong Lee, Sung Jae Shin, Won-Jung Koh, Byung Woo Jhun
Summary: This study evaluated the association between rrs mutations and amikacin resistance in clinical isolates of NTM in NTM-PD patients. The findings showed a higher prevalence of rrs mutations in high-level amikacin-resistant MAC isolates compared to low-level isolates, while all amikacin-resistant M. abscessus isolates had rrs mutations. These results emphasize the significance of phenotypic and genotypic susceptibility testing in managing MAC and M. abscessus-PD.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Dana Auganova, Sabina Atavliyeva, Asylulan Amirgazin, Akmaral Akisheva, Anna Tsepke, Pavel Tarlykov
Summary: In this study, whole-genome sequencing was used to examine drug resistance, compensatory mutations, population structure, and transmission patterns in clinical isolates of L2/Beijing Mtb collected in Kazakhstan. The study found that the genotypic prediction of Mtb susceptibility to anti-TB agents was consistent with the phenotypic susceptibility, except for bedaquiline. Most of the isolates were characterized as pre-extensively drug-resistant tuberculosis (pre-XDR-TB), and the high prevalence of the Central Asia outbreak (CAO) clade in the population structure of Mtb may explain the rapid spread of MDR-TB in Kazakhstan.
Article
Infectious Diseases
Dingwen Lin, Junning Wang, Zhezhe Cui, Jing Ou, Liwen Huang, Ya Wang
Summary: The study sequenced genomes of M. tuberculosis from high and low incidence regions in Guangxi, revealing significant genetic variation among populations in different counties. Regions with low TB incidence showed lower prevalence of Beijing genotypes, and strains of modern Beijing lineage were more likely to acquire drug resistance. The findings suggest a potential impact of bacterial population structure on TB incidence rates and support the hypothesis that the Modern Beijing lineage originated in South China.
BMC INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Anastasia Ushtanit, Yulia Mikhailova, Ludmila Krylova, Dmitry Grigorash, Marina Makarova, Svetlana Safonova, Danila Zimenkov
Summary: The emergence of drug-resistant tuberculosis has led to the development of new drugs and the screening of analogues with better efficacy and lower toxicity. Perchlozone, targeting a step in the FAS-II cycle, has recently been approved for tuberculosis treatment in Russia. The study analyzed the phenotypic properties of primary clinical isolates of M. tuberculosis and identified molecular determinants of resistance to isoniazid, ethionamide, and perchlozone. The findings show variability in perchlozone susceptibility and the possibility of using phenotypic or molecular analysis for therapy in certain cases.
Review
Microbiology
Wen-Feng Cao, Er-Ling Leng, Shi-Min Liu, Yong-Liang Zhou, Chao-Qun Luo, Zheng-Bing Xiang, Wen Cai, Wei Rao, Fan Hu, Ping Zhang, An Wen
Summary: Tuberculous meningitis is the most common and deadly type of central nervous system tuberculosis. Early diagnosis is crucial for patient prognosis, but laboratory diagnosis is challenging. This review summarizes the latest research in microbiological and molecular diagnostics for tuberculous meningitis and discusses issues related to diagnostic effectiveness, laboratory infrastructure, testing costs, and clinical expertise.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Nicholas C. Poulton, Zachary A. Azadian, Michael A. DeJesus, Jeremy M. Rock
Summary: Tuberculosis is a fatal bacterial infection, causing 1.5 million deaths globally each year. Drug-resistant strains of Mycobacterium tuberculosis (Mtb) have emerged, leading to efforts in developing novel drugs. The arabinogalactan biosynthetic enzyme DprE1 in Mtb is a promising drug target, with over a dozen inhibitory compounds identified. Among them, BTZ043 and PBTZ169 have shown promise and are in clinical trials. A study using CRISPRi chemical-genetic screen with PBTZ169 identified rv0678 as a negative regulator of the mmpS5/L5 drug efflux pump, which confers resistance to PBTZ169. Mutations in rv0678 are associated with resistance to another drug, bedaquiline. These results highlight the importance of monitoring for rv0678 mutations in ongoing clinical trials of BTZ043 and PBTZ169.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biology
Fabrizio Menardo, Caroline Colijn
Summary: Detecting factors associated with transmission is crucial for understanding disease epidemics and designing effective public health measures. In this study, the author used a simulation-based approach to investigate the influence of various epidemiological processes on clustering and TBL analyses of Mycobacterium tuberculosis genomic data sets. The results show that different factors, except the length of the infectious period, affect the outcomes of clustering and TBL distributions. The study also highlights the limitations of current interpretations of these analyses and suggests that optimal clustering thresholds and TBL distributions may vary depending on epidemiological conditions.
Article
Microbiology
Matthew Silcocks, Xuling Chang, Nguyen Thuy Thuong Thuong, Youwen Qin, Dang Thi Minh Ha, Phan Vuong Khac Thai, Srinivasan Vijay, Do Dang Anh Thu, Vu Thi Ngoc Ha, Hoang Ngoc Nhung, Nguyen Huu Lan, Nguyen Thi Quynh Nhu, David Edwards, Artika Nath, Kym Pham, Nguyen Duc Bang, Tran Thi Hong Chau, Guy Thwaites, A. Dorothee Heemskerk, Chiea Chuen Khor, Yik Ying Teo, Michael Inouye, Rick Twee-Hee Ong, Maxine Caws, Kathryn E. Holt, Sarah J. Dunstan
Summary: In this study, the prevalence and evolutionary dynamics of drug resistance in Mycobacterium tuberculosis in Ho Chi Minh City, Vietnam were investigated. The accuracy of predicting drug resistance from sequencing data was assessed. The findings shed light on the transmission and evolution of drug resistance, as well as the need for rapid assays for drug resistance detection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Emmanuel Riviere, Lennert Verboven, Anzaan Dippenaar, Sander Goossens, Elise De Vos, Elizabeth Streicher, Bart Cuypers, Kris Laukens, Fathia Ben-Rached, Timothy C. Rodwell, Arnab Pain, Robin M. Warren, Tim H. Heupink, Annelies Van Rie
Summary: Studies have shown that variants in bedaquiline-resistance genes can occur in isolates from bedaquiline-naive patients. The prevalence of these variants in such patients is high, but the number of isolates exhibiting phenotypic resistance is very low. There is an association between these variants and the lineage of Mycobacterium tuberculosis, leading to a lineage-dependent difference in bedaquiline phenotype. Future studies should investigate the impact of these variants on the acquisition of bedaquiline resistance and treatment outcomes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Dian Ayu Eka Pitaloka, Arfan Arfan, Dwi Syah Fitra Ramadhan, Lidya Chaidir
Summary: This study aims to evaluate the impact of mutations found in pyrazinamide monoresistant strains of Mycobacterium tuberculosis on the effectiveness of pyrazinamide for tuberculosis treatment. Five mutations of pyrazinamidase were analyzed, and the results showed that three of these mutations affected the enzyme's activity and led to PZA resistance. However, the other two mutations had no effect. Experimental clarification is needed for further studies on drug resistance in pyrazinamidase.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Jessica Comin, Jan Madacki, Isabel Rabanaque, Maria Zuniga-Anton, Daniel Ibarz, Alberto Cebollada, Jesus Vinuelas, Luis Torres, Juan Sahagun, Christophe Klopp, Jesus Gonzalo-Asensio, Roland Brosch, Maria-Jose Iglesias, Sofia Samper
Summary: Through analysis of a tuberculosis outbreak and molecular characteristics of the MtZ strain, several SNPs in genes related to virulence, pathogenesis, and survival, as well as other genomic polymorphisms, were identified, which may contribute to its success in transmission among the population.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Grace Mugumbate, Brilliant Nyathi, Albert Zindoga, Gadzikano Munyuki
Summary: This article discusses the role of computational techniques in understanding the drug resistance mechanisms of Mycobacterium tuberculosis, particularly the impact of mutations on the binding affinities of anti-TB drugs. With advancements and adoption of computational techniques, chemoinformatics, and bioinformatics, there are new methods for predicting mutations in Mtb.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Applied
Awat Wisetsai, Florian T. Schevenels, Kiatichai Faksri, Sarawut Tontapha, Vittaya Amornkitbamrung, Ratsami Lekphrom
Summary: Phytochemical investigation of the roots of Rothmannia wittii led to the isolation of three new iridoid glucosides and nine known compounds. The structures and configurations were determined through thorough analysis of mass spectrometric and NMR spectroscopic data, as well as CD calculations. Compounds 4 and 11 are reported from the Rubiaceae family for the first time.
NATURAL PRODUCT RESEARCH
(2022)
Letter
Infectious Diseases
K. Klaos, Y. Holicka, R. Groenheit, C. Kodmon, M. J. van der Werf, V. Nikolayevskyy
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2022)
Review
Infectious Diseases
Maria Krutikov, Lena Faust, Vladyslav Nikolayevskyy, Yohhei Hamada, Rishi K. Gupta, Daniela Cirillo, Alberto Matteelli, Alexei Korobitsyn, Claudia M. Denkinger, Molebogeng X. Rangaka
Summary: This study systematically assessed the diagnostic performance of novel skin-based tests for tuberculosis infection compared to the TST and IGRA tests. The results showed that the novel skin-based tests performed similarly to IGRA or TST, but there were variations in study quality.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Yen Holicka, Elisa Tagliani, Daniela Maria Cirillo, Vlad Nikolayevskyy
Summary: This study aims to gather observational evidence of the impact of Whole Genome Sequencing (WGS) in the pathogen identification, antimicrobial resistance profiling and transmission tracking of tuberculosis. The study found that WGS significantly improved TB drug resistance testing and reduced false clustering. Further research is needed to assess the financial impact of WGS.
Article
Microbiology
Orawee Kaewprasert, Sissades Tongsima, Rick Twee-Hee Ong, Kiatichai Faksri
Summary: This study demonstrates the importance of optimizing parameters and using high-quality variants in whole-genome sequence analysis for accurate bacterial phylogeny reconstruction.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Auttawit Sirichoat, Viraphong Lulitanond, Kiatichai Faksri
Summary: This study explores the microbial community composition and functional predictions of fermented fish (pla-ra) from Northeast Thailand. The results reveal the rich bacterial community with diverse metabolic pathways, as well as the presence of various genera in the fungal community. The study also uncovers an association between the microbial community and odor intensity.
ARCHIVES OF MICROBIOLOGY
(2022)
Editorial Material
Respiratory System
Sophia B. Georghiou, Timothy C. Rodwell, Alexei Korobitsyn, Said H. Abbadi, Kanchan Ajbani, Jan-Willem Alffenaar, David Alland, Nataly Alvarez, Sonke Andres, Elisa Ardizzoni, Alexandra Aubry, Rossella Baldan, Marie Ballif, Ivan Barilar, Erik C. Bottger, Soumitesh Chakravorty, Pauline M. Claxton, Daniela M. Cirillo, Inaki Comas, Chris Coulter, Claudia M. Denkinger, Brigitta Derendinger, Edward P. Desmond, Jurriaan E. M. de Steenwinkel, Keertan Dheda, Andreas H. Diacon, David L. Dolinger, Kelly E. Dooley, Matthias Egger, Maha R. Farhat, Lanfranco Fattorini, Iris Finci, Laure Fournier Le Ray, Victoria Furio, Ramona Groenheit, Tawanda Gumbo, Scott K. Heysell, Doris Hillemann, Harald Hoffmann, Po-Ren Hsueh, Yi Hu, Hairong Huang, Alamdar Hussain, Farzana Ismail, Kiyohiko Izumi, Tomasz Jagielski, John L. Johnson, Priti Kambli, Kone Kaniga, G. H. R. Eranga Karunaratne, Meenu Kaushal Sharma, Peter M. Keller, Ellis C. Kelly, Margarita Kholina, Mikashmi Kohli, Katharina Kranzer, Ian F. Laurenson, Jason Limberis, S-Y Grace Lin, Yongge Liu, Alexandre Lopez-Gavin, Anna Lyander, Diana Machado, Elena Martinez, Faisal Masood, Satoshi Mitarai, Nomonde R. Mvelase, Stefan Niemann, Vladyslav Nikolayevskyy, Florian P. Maurer, Matthias Merker, Paolo Miotto, Shaheed Omar, Ralf Otto-Knapp, Moises Palaci, Juan Jose Palacios Gutierrez, Sharon J. Peacock, Charles A. Peloquin, Jennifer Perera, Catherine Pierre-Audigier, Suporn Pholwat, James E. Posey, Therdsak Prammananan, Leen Rigouts, Jaime Robledo, Neesha Rockwood, Camilla Rodrigues, Max Salfinger, Marcos C. Schechter, Marva Seifert, Sarah Sengstake, Thomas Shinnick, Natalia Shubladze, Vitali Sintchenko, Frederick Sirgel, Sulochana Somasundaram, Timothy R. Sterling, Andrea Spitaleri, Elizabeth Streicher, Philip Supply, Erik Svensson, Elisa Tagliani, Sabira Tahseen, Akiko Takaki, Grant Theron, Gabriela Torrea, Armand Van Deun, Annelies Van Rie, Dick van Soolingen, Roger Vargas, Amour Venter, Nicolas Veziris, Cristina Villellas, Miguel Viveiros, Robin Warren, Shu'an Wen, Jim Werngren, Robert J. Wilkinson, Caie Yang, Tingting Zhang, Danila Zimenkov, Nazir Ismail, Thomas Schon, Claudio U. Koser, Soudeh Ehsani, Jakko van Ingen, F. Ferda Yilmaz
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Infectious Diseases
David Wyllie, Trien Do, Richard Myers, Vlad Nikolayevskyy, Derrick Crook, Tim Peto, Eliza Alexander, Esther Robinson, A. Sarah Walker, Colin Campbell, E. Grace Smith
Summary: Mixtures of transmissible strains occur in at least 5% of tuberculosis infections in England, and when present in pulmonary disease, such mixtures are associated with an increased risk of tuberculosis transmission.
JOURNAL OF INFECTION
(2022)
Article
Biochemical Research Methods
Francis Stieber, Nadia Allen, Kara Carpenter, Jenny Howard, Riccardo Alagna, Davide Manissero, Vladyslav Nikolayevskyy
Summary: Emerging evidence suggests the significant role of T-cells in COVID-19 immunity, and IGRA assays are considered attractive alternatives for accurate assessment. Adhering to good laboratory practice principles is important.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Immunology
Kanwara Trisakul, Ditthawat Nonghanphithak, Pratchakan Chaiyachat, Orawee Kaewprasert, Kankanon Sakmongkoljit, Wipa Reechaipichitkul, Angkana Chaiprasert, David Blair, Taane G. Clark, Kiatichai Faksri
Summary: Global susceptibility data for newly recommended anti-TB drugs against M/XDR-TB are limited. This study used whole-genome sequences to determine the proportion of XDR-TB isolates carrying mutations associated with resistance against these drugs.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biology
Orawee Kaewprasert, Ditthawat Nonghanphithak, Ploenchan Chetchotisakd, Wises Namwat, Rick Twee-Hee Ong, Kiatichai Faksri
Summary: In this study, we analyzed the genetic diversity and mutation rate of Mycobacterium abscessus between subspecies and identified drug-resistant mutations. We found a high mutation rate during the initial infection process, followed by a significant reduction in serial isolates separated by more than 180 days. There were also differences in mutation rate between the two subspecies, with MMAS strains showing higher susceptibility to certain antibiotics. The information obtained can be valuable in diagnosis and patient management.
Article
Microbiology
Ye Win Aung, Kiatichai Faksri, Arunnee Sangka, Kanchana Tomanakan, Wises Namwat
Summary: Tuberculosis (TB) is a leading cause of death worldwide. In this study, the sensitivity of droplet digital PCR (ddPCR) for diagnosing active pulmonary TB was compared with that of GeneXpert and AFB smear microscopy. The results showed that ddPCR had a sensitivity of 100%, higher than GeneXpert (82.4%) and AFB smear microscopy (41.9%). This study suggests that ddPCR has the potential to be used as an early diagnostic method for TB.
Article
Microbiology
Auttawit Sirichoat, Benjawan Kaewseekhao, Arnone Nithichanon, Sittiruk Roytrakul, Kiatichai Faksri
Summary: This study investigated the host proteomic patterns after clearance of Staphylococcus aureus from cells. The ATP8A2 protein was found to have potential as a clearance biomarker for S. aureus infection. Additionally, the expression of certain proteins varied between infection and clearance stages, suggesting their potential roles in S. aureus infection and clearance.
CURRENT MICROBIOLOGY
(2023)
Correction
Infectious Diseases
M. Krutikov, L. Faust, V Nikolayevskyy
LANCET INFECTIOUS DISEASES
(2022)
Correction
Infectious Diseases
M. Krutikov, L. Faust, V Nikolayevskyy
LANCET INFECTIOUS DISEASES
(2022)
