Article
Plant Sciences
Jing Han, Siwang Zhang, Baoping Jiang, Jingwen Wang, Xiaojing Ge, Bingrong Wu, Sen Zhang, Dongsheng Wang
Summary: The sesquiterpene lactones, including five major constituents, may be the main active constituent of X. sibiricum for its anti-asthma activity. SL-XS exerted its anti-asthma effect by modulating the Th1/Th2 balance via the JAK/STAT signaling pathway.
Article
Biochemistry & Molecular Biology
Maria Irene Cerezo-Cortes, Juan German Rodriguez-Castillo, Dulce Adriana Mata-Espinosa, Estela Isabel Bini, Jorge Barrios-Payan, Zyanya Lucia Zatarain-Barron, Juan Manuel Anzola, Fernanda Cornejo-Granados, Adrian Ochoa-Leyva, Patricia Del Portillo, Martha Isabel Murcia, Rogelio Hernandez-Pando
Summary: This study analyzed the transcriptomic differences between Beijing-Like SIT-190 and Beijing-Classic SIT-1 isolates of Mycobacterium tuberculosis in a murine model of progressive tuberculosis. The results showed differential transcriptional programs between these two strains, with Beijing-Like SIT-190 being associated with host immune response and hypoxia, and Beijing-Classic SIT-1 being associated with proteolysis and protein folding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Yee Huang, Li Nan, Chenwen Xiao, Jie Dong, Ke Li, Jvfen Cheng, Quanan Ji, Qiang Wei, Guolian Bao, Yan Liu
Summary: The study successfully developed GAN-OMV, which can stimulate Th1/Th2/Th17 immune responses against Bb and provide a promising strategy for novel vaccine development against the microbial pathogen. GAN-OMV showed a stable core-shell structure and effectively internalized into macrophages through multiple endocytotic pathways, promoting cell proliferation, cytokine secretion, and M1 polarization. Subcutaneous GAN-OMV vaccination significantly enhanced specific antibody production, lymphocyte proliferation, and the production of Th1/Th2/Th17 cytokines.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Immunology
Nazgol-Sadat Haddadi, Purvi Mande, Tia Y. Brodeur, Kaiyuan Hao, Grace E. Ryan, Stephanie Moses, Sharon Subramanian, Xhuliana Picari, Khashayar Afshari, Ann Marshak-Rothstein, Jillian M. Richmond
Summary: This study assessed the role of specific T cell subsets in the initiation and persistence of skin disease using a murine model of cutaneous lupus erythematosus (CLE). The findings suggest that Th2 cells trigger skin lesion formation in CLE, and these cells switch to a Th1-like phenotype over time. Furthermore, the study identified changes in T cells at four weeks post injection, with higher expression levels of interferon stimulated genes. These results highlight the importance of understanding the role of different T cell subsets in CLE progression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Michal Meir, Mark Foreman, Michal Bar-Oz, Noga Naor, Anna Rozenblit, Daniel Barkan
Summary: The study aimed to determine whether isolated glycopeptidolipid (GPL) defects affect the transmissibility of Mycobacterium abscessus. By comparing fully isogenic strains with and without GPL defects, the study found no survival advantage for either strain on fomites or in chlorhexidine. The findings suggest that reduced transmissibility of clinical isolates cannot be solely attributed to GPL synthesis mutations.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Liping Qin, Yu Song, Fan Zhang, Ru Wang, Li Zhou, Shiqi Jin, Chaojia Chen, Chunyang Li, Molin Wang, Baichun Jiang, Gongping Sun, Chunhong Ma, Yaoqin Gong, Peishan Li
Summary: CUL4B plays a critical role in Th cell differentiation through regulating histone modifications.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Maria Cristina Puzzolo, Ilaria Del Giudice, Nadia Peragine, Paola Mariglia, Maria Stefania De Propris, Luca Vincenzo Cappelli, Livio Trentin, Gianluigi Reda, Antonio Cuneo, Stefano Molica, Alfonso Piciocchi, Valentina Arena, Francesca Romana Mauro, Anna Guarini, Robin Foa
Summary: Research suggests that ibrutinib treatment can reverse T-cell polarization in chronic lymphocytic leukemia (CLL), decreasing Th2 cells and increasing Th1 cells, with a gradual reduction in the Th2/Th1 ratio after treatment initiation. Furthermore, patients with a Th2/Th1 ratio below 0.088 at M8 are more likely to achieve complete remission (CR).
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Philipp Burt, Michael Peine, Caroline Peine, Zuzanna Borek, Sebastian Serve, Michael Flossdorf, Ahmed N. Hegazy, Thomas Hoefer, Max Loehning, Kevin Thurley
Summary: Selective differentiation of CD4+ T helper (Th) cells into specialized subsets is crucial for appropriate immune responses. While canonical Th-cell lineages are well understood, the transcriptional architecture of hybrid phenotypes remains unclear. This study explored the transcriptome of Th-cell differentiation, revealing an early bifurcation point in gene expression programs. Hybrid Th1/2 cells showed mixed effects from Th1 and Th2 cells for a minority of genes, while others followed a Th1 and Th2 cell-independent transcriptional program. These findings emphasize the importance of longitudinal data for characterizing cellular phenotypes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Mercedes Fernandez-Escobar, Rafael Calero-Bernal, Javier Regidor-Cerrillo, Raquel Vallejo, Julio Benavides, Esther Collantes-Fernandez, Luis Miguel Ortega-Mora
Summary: The study identified significant intra- and inter-genotype virulence differences in Spanish T. gondii isolates, which were not reflected by the genetic characterization using currently described molecular markers. Isolates TgShSp16 (#3) and TgShSp24 (#2) presented higher degrees of virulence, and there were significant differences in invasion rates and tachyzoite yield at 72 h post-inoculation between isolates.
VETERINARY RESEARCH
(2021)
Review
Microbiology
Ekaterina Martynova, Albert Rizvanov, Richard A. Urbanowicz, Svetlana Khaiboullina
Summary: This review focuses on the mechanisms of inflammasome activation and their role in the development of Th1, Th2, and Th17 lymphocytes. The contribution of cytokines released from activated inflammasomes to the immune response is summarized, along with the discussion of the role of cytokines released from tissue cells in promoting lymphocyte differentiation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Tatiana Vinogradova, Marine Dogonadze, Natalia Zabolotnykh, Maria Badleeva, Irina Yarusova, Anna Vyazovaya, Alena Gerasimova, Svetlana Zhdanova, Maria Vitovskaya, Natalia Solovieva, Oksana Pasechnik, Oleg Ogarkov, Igor Mokrousov
Summary: Two newly discovered clusters of Mycobacterium tuberculosis strains of the early ancient sublineage of the Beijing genotype have emerged in the Asian part of Russia and are strongly associated with MDR/XDR. In murine model and in vitro growth experiments, the 396 strain from Buryatia, Far East, showed higher virulence, while the 6691 strain from Omsk, Siberia, exhibited lower virulence. Further study of the highly lethal MDR strain 396 representing the Beijing 14717-15 cluster in Russia is warranted.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Chemistry, Applied
Ishfaq Ahmed, Hong Lin, Zhenxing Li, Lili Xu, Ihsan Mabood Qazi, Chen Luo, Xiang Gao, Mati Ullah Khan, Amjad Iqbal, Yuman Guo, Tushar Ramesh Pavase, Lirui Sun
Summary: The study found that enzymatic cross-linking of shrimp tropomyosin with tyrosinase and caffeic acid could reduce allergic responses, improve digestibility, decrease IgG/IgE recognition, alleviate allergic symptoms, and inhibit allergic markers in the serum via immunosuppression and modulation of T-helper cell balance.
Review
Immunology
Weihang Luo, Jindong Hu, Weifang Xu, Jingcheng Dong
Summary: The immune response in the asthmatic respiratory tract is driven by CD4(+) T helper cells, especially Th2 cells. Asthma is a heterogeneous and progressive disease, with different stages of the disease course showing different immune responses. The complex immune networks in the inflammatory airway make it challenging to find a universal treatment for all asthmatics.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Yuan Zhang, Jiuli Zhang, Jun Bao, Chaohua Tang, Ziwei Zhang
Summary: The study found that selenium deficiency can lead to necroptosis, inflammatory responses, and abnormal expression of cytokines in swine intestine tissues, potentially causing damage to the digestive system.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Immunology
Xin Lv, Jian Wang, Li Zhang, Xian Shao, Yao Lin, Hongyan Liu, Guangyang Ma, Jing Li, Saijun Zhou, Pei Yu
Summary: This study investigated the mechanism by which the SGLT2 inhibitor canagliflozin attenuates podocyte injury in rats with idiopathic membranous nephropathy (MN). The results showed that canagliflozin improved kidney function, reduced proteinuria, and had a more pronounced protective effect on the kidneys compared to losartan. The mechanism involved reversing the imbalance in Th1/Th2 cells and restoring podocyte autophagy inhibited by abnormal immunoglobulin G secretion from B-cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Bertin C. Bisimwa, Jean B. Nachega, Robin M. Warren, Grant Theron, John Z. Metcalfe, Maunank Shah, Andreas H. Diacon, Nadia A. Sam-Agudu, Marcel Yotebieng, Andre N. H. Bulabula, Patrick D. M. C. Katoto, Jean-Paul Chirambiza, Rosette Nyota, Freddy M. Birembano, Eric M. Musafiri, Sifa Byadunia, Esto Bahizire, Michel K. Kaswa, Steven Callens, Zacharie M. Kashongwe
Summary: In a study conducted in eastern Democratic Republic of the Congo, it was found that Xpert had a high sensitivity for detecting RIF resistance but a low positive-predictive value for MDR-TB. Comprehensive resistance testing for both INH and RIF should be performed in this setting.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Grant Theron, Jason Limberis, Rouxjeane Venter, Liezel Smith, Elize Pietersen, Aliasgar Esmail, Greg Calligaro, Julian te Riele, Marianna de Kock, Paul van Helden, Tawanda Gumbo, Taane G. Clark, Kevin Fennelly, Robin Warren, Keertan Dheda
Review
Genetics & Heredity
Julian S. Peters, Nabila Ismail, Anzaan Dippenaar, Shuyi Ma, David R. Sherman, Robin M. Warren, Bavesh D. Kana
ANNUAL REVIEW OF GENETICS, VOL 54, 2020
(2020)
Article
Microbiology
Maxwell T. Chirehwa, Richard Court, Mariana de Kock, Lubbe Wiesner, Nihal de Vries, Joseph Harding, Tawanda Gumbo, Gary Maartens, Rob Warren, Paolo Denti, Helen McIlleron
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Biochemical Research Methods
Emmanuel Riviere, Tim H. Heupink, Nabila Ismail, Anzaan Dippenaar, Charlene Clarke, Gemeda Abebe, Peter Heusden, Rob Warren, Conor J. Meehan, Annelies Van Rie
Summary: Asia and Africa are underrepresented in Mtb WGS research compared to their TB burden, leading to adaptations in training, mentoring, and infrastructure building. Utilizing case studies created a safe space for students to voice and defend their opinions. Ensuring quality DNA extraction, biosafety level 3 and general laboratory skill training, commercial DNA extraction kits, and a combination of training in a highly equipped laboratory and local setting were added to the program.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Multidisciplinary Sciences
Wynand J. Goosen, Tanya J. Kerr, Leanie Kleynhans, Robin M. Warren, Paul D. van Helden, David H. Persing, Sven D. C. Parsons, Peter Buss, Michele A. Miller
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Albany Resendiz-Mora, Carlos Wong-Baeza, Irene Nevarez-Lechuga, Carla Landa-Saldivar, Erendira Molina-Gomez, Rogelio Hernandez-Pando, Isabel Wong-Baeza, Alejandro Escobar-Gutierrez, Isabel Baeza
Summary: The study evaluated the role of the TH2 response, particularly the cytokine IL-4, in the development of a lupus-like disease in mice induced by NPAs stabilization. The findings indicated that IL-4 plays a central role in this process.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Katrin Smith, Netanya Bernitz, Samantha Goldswain, David V. Cooper, Robin M. Warren, Wynand J. Goosen, Michele A. Miller
Summary: The study evaluated the Mabtech ELISA kit for detecting buffalo IFN-γ, showing good sensitivity and specificity for early detection of Mycobacterium bovis. The Mabtech IGRA demonstrated a sensitivity of 89% and a specificity of 94%, indicating it as a promising diagnostic tool for M. bovis detection in African buffaloes.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Immunology
Katrin Smith, Leanie Kleynhans, Candice Snyders, Netanya Bernitz, David Cooper, Paul van Helden, Robin M. Warren, Michele A. Miller, Wynand J. Goosen
Summary: The African buffalo as a maintenance host for Mycobacterium bovis poses transmission risks to livestock, humans, and other wildlife. Using the MILLIPLEX(R) platform to test whole blood samples from infected buffaloes revealed potential cytokine biomarkers and demonstrated the utility of the platform in this novel species. Further investigation is needed to confirm these preliminary findings.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Immunology
Josephine Chileshe, Tanya J. Kerr, Craig Kinnear, Peter E. Buss, Paul D. van Helden, Robin M. Warren, Michele A. Miller, Sven D. C. Parsons
Summary: This study aimed to evaluate a novel unbiased approach to discovering candidate biomarkers for M. bovis infection in rhinoceros and to conduct preliminary validation. Analysis of gene expression in immune sensitized rhinoceros samples identified several genes upregulated in response to antigen stimulation, leading to the design of new gene expression assays for selected candidates. The findings confirm the value of the equine RT2 profiler PCR array as a useful tool for screening biomarkers for the diagnosis of M. bovis infection in rhinoceros.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Microbiology
Jacqueline V. Lara-Espinosa, Maria Fernanda Arce-Aceves, Jorge Barrios-Payan, Dulce Mata-Espinosa, Vasti Lozano-Ordaz, Enrique Becerril-Villanueva, Maria Dolores Ponce-Regalado, Rogelio Hernandez-Pando
Summary: This study found that low doses of dexamethasone can reduce the bacilli load and pneumonia in tuberculosis-infected lungs and increase animal survival. Dexamethasone also decreases the inflammatory response and neurological abnormalities in infected animals. Therefore, dexamethasone represents a promising adjuvant treatment for pulmonary tuberculosis.
Article
Biochemistry & Molecular Biology
Jose L. Sanchez-Gloria, Constanza E. Martinez-Olivares, Leonardo Del Valle-Mondragon, Fernando Cortes-Camacho, Oscar R. Zambrano-Vasquez, Rogelio Hernandez-Pando, Fausto Sanchez-Munoz, Laura G. Sanchez-Lozada, Horacio Osorio-Alonso
Summary: In this study, we evaluated the effects of allicin on vascular remodeling, endothelial function, and oxidative stress in experimental pulmonary arterial hypertension (PAH). Allicin administration improved body weight, survival rate, and prevented wall thickness and right ventricle hypertrophy. Allicin also maintained normal levels of angiotensin II and its receptors, increased nitric oxide and other beneficial molecules, and prevented oxidative stress and hypoxia response. These effects of allicin contribute to preventing endothelial dysfunction, remodeling of the pulmonary arteries, and heart failure in PAH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Belen Cuevas-Lopez, Edgar Ignacio Romero-Ramirez, Fernando E. Garcia-Arroyo, Edilia Tapia, Juan Carlos Leon-Contreras, Alejandro Silva-Palacios, Francisco-Javier Roldan, Omar Noel Medina Campos, Luz Hernandez-Esquivel, Alvaro Marin-Hernandez, Jose Guillermo Gonzaga-Sanchez, Rogelio Hernandez-Pando, Jose Pedraza-Chaverri, Laura Gabriela Sanchez-Lozada, Omar Emiliano Aparicio-Trejo
Summary: The incidence of kidney disease is increasing globally, and acute kidney injury (AKI) can contribute to the development of cardio-renal syndrome (CRS) type 3. However, the mechanism behind CRS development is not fully understood. This study aimed to investigate the molecular mechanisms associated with cardiac mitochondrial impairment and its role in CRS development. The findings suggest that mitochondrial bioenergetics impairment plays a crucial role in heart damage, and the preservation of heart mitochondrial function during AKI could be a valuable strategy to prevent CRS type 3.
Article
Biochemistry & Molecular Biology
Angelica Flores-Flores, Samuel Estrada-Soto, Cesar Millan-Pacheco, Blanca Bazan-Perkins, Rogelio Hernandez-Pando, Maximiliano Ibarra-Barajas, Rafael Villalobos-Molina
Summary: Asthma is a condition where the airways become inflamed and narrowed, causing symptoms such as shortness of breath and wheezing. It was found that 6-aminoflavone (6-NH2F) can effectively relax the airways and has low toxicity in animal models, potentially through calcium channel blockade.
Review
Chemistry, Medicinal
Dulce Mata-Espinosa, Jacqueline V. Lara-Espinosa, Jorge Barrios-Payan, Rogelio Hernandez-Pando
Summary: This study reviews the model strategies for gene therapy and new vaccines in the treatment of tuberculosis, suggesting that it is a potential alternative therapy for active TB, as well as for the prevention and treatment of latent TB reactivation and transmission of Mtb.