Review
Immunology
Zhu-xing Ji, Xiao-quan Wang, Xiu-fan Liu
Summary: Since the 1918 influenza pandemic, people have recognized the perniciousness of the virus. The NS1 protein plays a key role in the influenza virus's interaction with the host's innate immunity, affecting the virus's pathogenicity and its interaction with the host.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Fisheries
Chang Min Kim, Hyunseok Jang, Eunmi Hong, Jun Hyuck Lee, Hyun Ho Park
Summary: In this study, the TRAF4 gene was cloned and purified from the Antarctic yellowbelly rockcod. The crystal structure of teleost fish TRAF4 was determined, and the mechanisms of cold adaptiveness of signaling molecules were elucidated. The interaction between the platelet receptor GPIb beta homolog and ncTRAF4 was also identified, indicating the role of ncTRAF4 in mediating cellular signaling. These findings provide new insights into TRAF4-mediated immune cell and platelet signaling in fish and the cold adaptiveness of signaling molecules.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Gyu Hwan Hyun, In Ho Cho, Yoon Young Yang, Da-Hye Jeong, Yun Pyo Kang, You-Sun Kim, Seul Ji Lee, Sung Won Kwon
Summary: This study investigates the immunological action of complex pectin by studying its interaction with Toll-like receptors (TLRs). The research shows that pectic heteropolysaccharides (HPSs) derived from terrestrial plant cell walls can bind to TLR4 and activate the immune response. The findings provide a better understanding of the interaction between complex carbohydrates and proteins.
CARBOHYDRATE POLYMERS
(2023)
Article
Microbiology
Quanjie Li, Ni An, Xiao Yin, Ruixin Zhang, Huihan Shao, Dongrong Yi, Shan Cen
Summary: This study characterizes the binding mechanism of MxB protein to NS5A of hepatitis C virus and reveals the key residues involved. The presence of MxB weakens the association between NS5A and cyclophilin A, leading to a decrease in their binding affinity. This work provides insights into the antiviral mechanism of MxB and may contribute to the development of new strategies against CypA-dependent viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Xiaokai Bao, Weijun Wang, Tingzhu Yuan, Yan Li, Xipan Chen, Xiumei Liu, Xiaohui Xu, Guohua Sun, Bin Li, Jianmin Yang, Yanwei Feng, Zan Li
Summary: This study investigated the immune response of Amphioctopus fangsiao larvae infected with Vibrio anguillarum under different egg-protecting behaviors. Transcriptome analysis identified thousands of differentially expressed genes and revealed enriched immune-related GO terms and KEGG signaling pathways. Protein-protein interaction networks helped identify hub genes related to multiple gene functions and signaling pathways.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yukai Qin, Zhi Luo, Ke Zhao, Xingyu Nan, Yanan Guo, Weiwei Li, Qun Wang
Summary: This study identified a novel SVWC gene, named EsSVWC, in Eriocheir sinensis, which responds positively to bacterial stimulation and regulates the expression of antimicrobial peptides, thus improving the crab's resistance to bacterial infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Zan Li, Xiaokai Bao, Xintian Liu, Yan Li, Mingxian Cui, Xiumei Liu, Bin Li, Yanwei Feng, Xiaohui Xu, Guohua Sun, Weijun Wang, Jianmin Yang
Summary: The protection provided by the immune system is essential for the survival of organisms. Problems within the immune network can impact the organism's normal activities. Octopus ocellatus is a widely distributed cephalopod species in the world's oceans, and research on its unique nervous system and locomotive organs has been increasing in recent years. In this study, we analyzed gene expression profiles in O. ocellatus larvae and identified 5936 differentially expressed genes (DEGs). Protein-protein interaction networks were constructed to examine immune-related DEGs, leading to the identification of 15 hub genes involved in multiple signaling pathways and protein interactions. This study sheds light on the impact of egg protection on the immunity of O. ocellatus larvae and provides valuable genetic resources for understanding invertebrate immunity.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Kexin Liu, Zhe Qu, Jingjie Hu, Zhenmin Bao, Mengqiang Wang
Summary: In this study, the QM protein gene was cloned from Eriocheir sinensis and found to play important roles in immune responses. The gene showed responses to bacterial infection and environmental pollution.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Kifayatullah Mengal, Golara Kor, Valentina Siino, Milos Buric, Pavel Kozak, Fredrik Levander, Hamid Niksirat
Summary: Hemolymph in crustaceans plays a crucial role in wound healing and immune responses. A comparative analysis of the protein composition between non-clotted and clotted hemolymph in crayfish was conducted. The majority of proteins showed no significant changes during coagulation, while four proteins displayed abundance differences that may affect the coagulation process and the phagocytosis ability of hemocytes.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hailun Liu, Yuge Li, Yilong Hu, Yuhua Yang, Wenbin Zhang, Ming He, Xiaoming Li, Chunyu Zhang, Fanjiang Kong, Xu Liu, Xingliang Hou
Summary: EIJ1 acts as a negative regulator of plant immunity by interacting with EDS1 and inhibiting pathogen-triggered trafficking of EDS1 to the nucleus. As disease progresses, EIJ1 is degraded, allowing EDS1 to accumulate in the nucleus for enhanced resistance. The avirulent strain Pst DC3000 (AvrRps4) induces rapid degradation of EIJ1 to abolish its repressive effect during effector-triggered immunity.
Article
Oncology
Wenyong Huang, Dongmei Ye, Wenjing He, Xiaoshun He, Xiaomin Shi, Yifang Gao
Summary: The study found that MAIT cells in patients with HCC had lower levels in both peripheral blood and liver compared to healthy controls, with reduced IFN-γ production. While peripheral MAIT cells showed upregulation of HLA-DR and PD-1, intrasinusoidal MAIT cells did not exhibit significant differences. Additionally, MAIT cells were significantly enriched in the livers of HCC patients, showing high levels of activation and exhaustion markers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Editorial Material
Immunology
Xinxin Wang, Yuping Lai
Summary: In this study, the authors investigate the mechanism by which pattern recognition receptors NOD1 and NOD2 sense bacterial muropeptides to induce inflammation in keratinocytes. They demonstrate that solute carrier 46A2 (SLC46A2) transports DAP-muropeptides into the cytosol, leading to NOD1 activation and subsequent skin inflammation during psoriasis.
Review
Immunology
Muhammad Nadeem Abbas, Saima Kausar, Bibi Asma, Wenhao Ran, Jingui Li, Zini Lin, Tiejun Li, Hongjuan Cui
Summary: The interaction between bacteria and insects has widespread implications due to their global distribution. It can directly impact human health as insects serve as disease vectors, and also have economic consequences. Moreover, it is linked to high mortality rates in economically important insects, resulting in significant economic losses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Xiongnan Chen, Xiaojun Chen, Yifan Liang, Sijia Xu, Zhijun Weng, Qi Gao, Zhao Huang, Guihong Zhang, Lang Gong
Summary: In this study, we identified the interaction between African swine fever virus (ASFV) structural protein p30 and host cell proteins using a yeast two-hybrid system. We further analyzed the functional enrichment of the host proteins and their impact on host cell functions. This study enhances our understanding of the interaction between ASFV and the host, and provides valuable information for future antiviral research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Minfeng Yang, Arpan Mahanty, Chunjing Jin, Alex Ngai Nick Wong, Jung Sun Yoo
Summary: This study established a label-free metabolic intravital imaging technique using two coenzymes as robust imaging markers to monitor metabolic responses to immunotherapy. The results demonstrated the sensitive monitoring capability of the imaging technique for therapeutic response and the ability to differentiate cancer cells and immunosuppressive cells.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Cell Biology
D. A. B. Rex, Nupur Agarwal, T. S. Keshava Prasad, Richard K. Kandasamy, Yashwanth Subbannayya, Sneha M. Pinto
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2020)
Article
Multidisciplinary Sciences
Leonhard X. Heinz, JangEun Lee, Utkarsh Kapoor, Felix Kartnig, Vitaly Sedlyarov, Konstantinos Papakostas, Adrian Cesar-Razquin, Patrick Essletzbichler, Ulrich Goldmann, Adrijana Stefanovic, Johannes W. Bigenzahn, Stefania Scorzoni, Mattia D. Pizzagalli, Ariel Bensimon, Andre C. Mueller, F. James King, Li Jun, Enrico Girardi, M. Lamine Mbow, Charles E. Whitehurst, Manuele Rebsamen, Giulio Superti-Furga
Article
Biochemistry & Molecular Biology
Mattia D. Pizzagalli, Ariel Bensimon, Giulio Superti-Furga
Summary: This review provides an overview of the SLC superfamily of transporter proteins and their roles in regulating metabolism and environmental conditions at the cell surface, with a focus on their involvement in cancer development and progression.
Article
Biochemistry & Molecular Biology
Yashwanth Subbannayya, Markus Haug, Sneha M. Pinto, Varshasnata Mohanty, Hany Zakaria Meas, Trude Helen Flo, T. S. Keshava Prasad, Richard K. Kandasamy
Summary: This study conducted label-free high-resolution proteomic profiling of resting and activated CD4+ T cells, identifying significant alterations in the levels of numerous proteins and observed changes in stimulatory/inhibitory immune checkpoint markers between the two states. Network analysis revealed regulatory hubs of CD4+ T cell activation, and comparisons with other cell types indicated interspecies proteomic differences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
D. A. B. Rex, Shobha Dagamajalu, Richard K. Kandasamy, Rajesh Raju, T. S. Keshava Prasad
Summary: COVID-19, caused by SARS-CoV-2, varies in clinical manifestations and disease progression; understanding the mechanism of SARS-CoV-2 interaction with host cells is crucial for developing novel therapeutics.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2021)
Article
Multidisciplinary Sciences
Hera Kim, Yashwanth Subbannayya, Fiachra Humphries, Astrid Skejsol, Sneha M. Pinto, Miriam Giambelluca, Terje Espevik, Katherine A. Fitzgerald, Richard K. Kandasamy
Summary: This study investigated the gene expression changes in THP1-derived macrophages upon TLR4 activation and identified several interferon-stimulated genes, including CMPK2. The in-depth characterization of CMPK2 expression revealed its significant induction upon TLR4 and TLR3 ligand stimulation, suggesting its potential role in anti-bacterial and antiviral responses mediated by IRF3-type I interferon signaling.
Article
Cell Biology
D. A. B. Rex, K. Deepak, Neelanchal Vaid, Shobha Dagamajalu, Richard Kumaran Kandasamy, Trude Helen Flo, T. S. Keshava Prasad
Summary: Bradykinin signals through its receptor B2R with diverse vascular permeability functions and is implicated in inflammation, chronic pain, vasculopathy, neuropathy, obesity, diabetes, and cancer. An integrated pathway reaction map of bradykinin consisting of 233 reactions has been developed, which will aid in identifying novel therapeutic targets for diseases associated with dysregulated bradykinin signaling.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Anjana Soorajkumar, Ebrahim Alakraf, Mohammed Uddin, Stefan S. Du Plessis, Alawi Alsheikh-Ali, Richard K. Kandasamy
Summary: Short linear motifs (SLiMs) are important for mediating protein-protein interactions, and viruses utilize these motifs to disrupt the host system. Through sequence analysis, four overriding SLiMs were identified in the SARS-CoV-2 Omicron variant, which may interfere with immune functions, cellular pathways, and contribute to viral infection and transmission. These SLiMs serve as potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Devasahayam Arokia Balaya Rex, Thottethodi Subrahmanya Keshava Prasad, Richard K. Kandasamy
Summary: The fate of a viral infection in the host is determined by various cellular responses, including different types of cell death mechanisms. Apoptosis, necroptosis, and pyroptosis play crucial roles in the response to viral infections. This review discusses the mechanisms by which viruses interfere with cell death and illustrates the general principles and cellular signaling mechanisms of virus-host molecule interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Evandro Ferrada, Giulio Superti-Furga
Summary: This study provides an integrative classification of solute carriers by combining evolutionary information with proteome-wide structure models. The results reveal approximately 180 independent evolutionary origins of solute carriers and identify 24 structurally distinct transmembrane folds. In addition, a new solute carrier member and details of noncanonical solute carriers are discovered.
Article
Biology
Manuele Rebsamen, Enrico Girardi, Vitaly Sedlyarov, Stefania Scorzoni, Konstantinos Papakostas, Manuela Vollert, Justyna Konecka, Bettina Guertl, Kristaps Klavins, Tabea Wiedmer, Giulio Superti-Furga
Summary: This study developed a strategy to infer functional relationships between solute carrier (SLC) transporters and metabolites, revealing that up-regulation of transport activity may overcome environmental metabolic restrictions.
LIFE SCIENCE ALLIANCE
(2022)
Article
Multidisciplinary Sciences
Sneha M. Pinto, Yashwanth Subbannayya, Hera Kim, Lars Hagen, Maria W. Gorna, Anni I. Nieminen, Magnar Bjoras, Terje Espevik, Denis Kainov, Richard K. Kandasamy
Summary: The COVID-19 pandemic remains a global health concern due to the emergence of new variants. Extensive evidence from multi-Omics studies has revealed host-pathogen interactions and potential therapeutic targets. Understanding the regulation of host signaling networks by post-translational modifications and their impact on cellular dynamics is crucial for advancing knowledge on SARS-CoV-2 infections.
Article
Biology
Patrick Essletzbichler, Vitaly Sedlyarov, Fabian Frommelt, Didier Soulat, Leonhard X. Heinz, Adrijana Stefanovic, Benedikt Neumayer, Giulio Superti-Furga
Summary: Phagocytosis is a crucial process for tissue clearance and host defense, but its dysregulation is linked to various diseases. This study used a genome-wide CRISPRko FACS screen to identify 716 genes involved in phagocytosis. By mapping the hits to a protein-protein interaction network, novel phagocytosis regulators were discovered and validated. This comprehensive network provides insights into cargo uptake and transformation, and can aid in the identification of potential drivers for endo-lysosomal diseases.
LIFE SCIENCE ALLIANCE
(2023)
Review
Pharmacology & Pharmacy
Vojtech Dvorak, Giulio Superti-Furga
Summary: Solute carriers (SLCs) are the largest group of membrane transporters in the human genome, and they are central in controlling metabolism compartmentalization, with most of this superfamily being linked to human diseases. Despite being considered attractive therapeutic targets, many SLCs still lack functional and structural annotations.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Cell Biology
Haobo Zhang, Lea Bernaleau, Maeva Delacretaz, Ed Hasanovic, Ales Drobek, Hermann Eibel, Manuele Rebsamen
Summary: Endolysosomal Toll-like receptors (TLRs) have important roles in immune responses and autoimmune diseases. SLC15A4 is essential for TLR7/8/9-induced responses and disease development in SLE. SLC15A4 recruits TASL to endolysosomes, leading to the activation of IRF5 and production of interferon b and cytokines. Targeting the SLC15A4-TASL complex could be a potential therapeutic strategy for SLE and related diseases.