期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 9, 期 -, 页码 40-47出版社
ELSEVIER
DOI: 10.1016/j.algal.2015.02.023
关键词
Aurantiochytrium; Thraustochytrid; Docosahexaenoic acid; Polyunsaturated fatty acids; Omega-3 alternative
资金
- Ministry of Higher Education Malaysia under the Exploratory Research Grant Scheme [ERGS/1/2012/STG08/UKM/02/15]
Thraustochytrids are marine protists belonging to the class Labyrinthula of the kingdom Chromista. This group of microbes is considered a promising alternative source of high-valued omega-3 oils, especially docosahexaenoic acid (DHA, C22:6n3), replacing fish oil which is the current major source of DHA and eicosapentaenoic acid (EPA, C20:5n3). Our new isolate, SW1, was considered to be an Aurantiochytrium based on its morphology, fatty acid profile and molecular phylogenetic analysis. Microscopic observations revealed that SW1 has high similarity to Aurantiochytrium limacinum ATCC MYA-1381 (SR21), possessing spherical vegetative cells which undergo repeated bipartition to form diads, tetrads, octads and zoosporangia that release motile zoospores. When cultivated in four different media, SW1 produced the highest biomass (13.17 g/L) and DHA (3.6 g/L) in a medium composed of glucose, sea salt, monosodium glutamate (MSG) and yeast extract. Visualization of lipid droplet development in SW1 using Sudan Black B dye revealed that lipid droplets enlarge to occupy almost the entire cell volume within 72 to 96 h of cultivation. This strain was also found to be able to utilize various saccharides as carbon source. The results of this study show that Aurantiochytrium sp. SW1 is a potential candidate to be developed as commercial microbial DHA producer. (C) 2015 Elsevier B.V. All rights reserved.
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