Article
Biochemistry & Molecular Biology
Ireneusz Litwin, Seweryn Mucha, Ewa Pilarczyk, Robert Wysocki, Ewa Maciaszczyk-Dziubinska
Summary: In this study, it was found that trivalent antimony can cause various forms of DNA damage, including replication and oxidative DNA damage, and affect the activation of DNA damage checkpoints and formation of recombination repair centers in the model organism Saccharomyces cerevisiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yinjie Su, Bo Wang, Jian Huang, Ming Huang, Tianxin Lin
Summary: The study aimed to evaluate PTEN regulation and identify targets for relieving chemoresistance in bladder cancer. The expression of YTHDC1 was found to be associated with cisplatin sensitivity in patients with bladder cancer. Decreased YTHDC1 expression promotes cisplatin resistance, while overexpression of YTHDC1 enhances cisplatin sensitivity. Furthermore, reducing YTHDC1 expression activates DNA damage response, including faster cell cycle recovery, apoptosis evasion, and enhanced DNA repair capability.
CELL PROLIFERATION
(2023)
Article
Oncology
Job-Joris Meijer, Alessandro Leonetti, Giulia Airo, Marcello Tiseo, Christian Rolfo, Elisa Giovannetti, Mahrou Vahabi
Summary: Despite poor prognosis for SCLC patients, immunotherapeutic approaches show potential. Targeting aberrant signaling pathways with new agents has shown promising results, while epigenetic alterations, gene amplifications, and mutations can act as potential biomarkers. Further research and clinical translational studies can help identify specific predictive biomarkers.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Neurosciences
Chao Liu, Shuwen Kuang, Lei Wu, Quan Cheng, Xuan Gong, Jun Wu, Longbo Zhang
Summary: H3(K27M) mutated diffuse midline gliomas (DMGs) are highly aggressive and major cause of cancer-related deaths in pediatric brain tumors, with common radio-resistance. This study summarized current understandings of the molecular responses of H3(K27M) DMGs to radiotherapy and provided crucial insights into current advances in radiosensitivity enhancement.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Genetics & Heredity
Delisa E. Clay, Donald T. Fox
Summary: Genome damage is a common threat to all organisms, and DNA damage responses (DDRs) play a crucial role in initiating cell cycle arrest, repair, and cell death. While many DDR components are conserved across different organisms, there are also adaptations to specific needs. Research on model genetic organisms has greatly advanced our understanding of DDR mechanisms and how they are influenced by the cell cycle stage. Future studies expanding beyond traditional model organisms will further enhance our knowledge of genome protection mechanisms.
Article
Cell Biology
Ilaria Ceppi, Elda Cannavo, Helene Bret, Rosa Camarillo, Francesca Vivalda, Roshan Singh Thakur, Amador Romero-Franco, Alessandro A. Sartori, Pablo Huertas, Raphael Guerois, Petr Cejka
Summary: In this study, the researchers used AlphaFold2 to identify a separation-of-function mutant of CtIP, CtIP-F728E-Y736E, which can still work with MRN but cannot stimulate ssDNA degradation by DNA2. The findings support a model in which the phosphorylation of CtIP by CDK activates DNA resection in the S phase, while the phosphorylation of CtIP by PLK1 disrupts its stimulation of DNA2, attenuating long-range resection later in the cell cycle.
GENES & DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Anjali Mann, Miguel Angel Ramirez-Otero, Anna De Antoni, Yodhara Wijesekara Hanthi, Vincenzo Sannino, Giorgio Baldi, Lucia Falbo, Anna Schrempf, Sara Bernardo, Joanna Loizou, Vincenzo Costanzo
Summary: POLO plays an important role in repairing DNA double-strand breaks in HR-defective tumors. It is found that POLO processes stalled Okazaki fragments, preventing the accumulation of ssDNA gaps on lagging strands in the absence of RAD51. Inhibition of POLO's DNA polymerase activity leads to unprotected fork gaps, which are cleaved by the MRE11-NBS1-CtIP endonuclease, causing asymmetric single-ended DSBs that impede the survival of BRCA2-defective cells.
Review
Endocrinology & Metabolism
Yan Wang, Mengrong Su, Yujie Chen, Xinyu Huang, Lian Ruan, Qizhuang Lv, Li Li
Summary: This study provides a systematic review of the role and mechanism of DNA damage repair in germ cell development. It summarizes the causes, detection methods, and repair methods of DNA damage, as well as the mechanism of DNA damage repair. It also discusses the causes of abnormal DNA damage repair in germ cells, the effects of germ cell damage, and the applications of DNA damage repair in reproductive diseases and related surgical treatments.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Environmental
Hongrui Guo, Yujuan Ouyang, Jiaqi Wang, Hengmin Cui, Huidan Deng, Xinyue Zhong, Zhijie Jian, Huan Liu, Jing Fang, Zhicai Zuo, Xun Wang, Ling Zhao, Yi Geng, Ping Ouyang, Huaqiao Tang
Summary: Copper is essential but over-exposure can lead to adverse health effects. CuSO4 was found to induce spermatogenesis disorder through oxidative stress-mediated DNA damage and apoptosis, impairing male reproductive function.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Virology
Michelle Mac, Brianna M. M. DeVico, Sophia M. M. Raspanti, Cary A. A. Moody
Summary: In this study, the researchers found that SETD2-mediated H3K36me3 promotes the repair of damaged DNA on HPV31 chromatin by facilitating the recruitment of CtIP and Rad51 to viral DNA through LEDGF binding to H3K36me3. SETD2 trimethylates H3K36me3 during transcription, and active transcription is necessary for Rad51 recruitment to viral DNA.
JOURNAL OF VIROLOGY
(2023)
Article
Oncology
Lifang Pan, Qiong Wu, Yuqing Wang, Shenglin Ma, Shirong Zhang
Summary: This study aimed to establish and explore the biological characteristics of radioresistant LUSC cells. Radioresistant cells showed decreased radiosensitivity, enhanced DNA damage repair ability, and regulated double strands break through the ATM/CHK2 and DNA-PKcs/Ku70 pathways. Proteomics analysis revealed that differential genes in radioresistant cells were mainly enriched in biological pathways such as cell migration and ECM-receptor interaction.
Editorial Material
Plant Sciences
Moira Rodriguez, Ana Martinez-Hottovy, Alan C. Christensen
Summary: Plant mtDNA can be repaired through homologous recombination, and mitochondrial interactions may influence genetic behavior.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biology
Sissel Hauge, Adrian Eek Mariampillai, Gro Elise Rodland, Lilli T. E. Bay, Helga B. Landsverk, Randi G. Syljuasen
Summary: The combination of cell cycle checkpoint inhibitors and radiation therapy can disrupt checkpoints, induce mitotic catastrophe, selectively increase the radiosensitivity of tumor cells, and inhibit DNA repair. In addition, these kinase inhibitors can also induce significant replication stress in the S phase of the cell cycle, contributing to the elimination of radioresistant S phase cells. Recent studies have also found that the combination of cell cycle checkpoint inhibitors and radiation therapy can enhance anti-tumor immune effects.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Oncology
Wenfeng Gou, Xiaojun Yu, Shaohua Wu, Hongying Wu, Huajie Chang, Leyuan Chen, Huiqiang Wei, Changfen Bi, Hongxin Ning, Yingliang Wu, Wenbin Hou, Daiying Zuo, Yiliang Li
Summary: This study investigated the impact of AND-1 on the radiosensitivity of non-small cell lung cancer (NSCLC) and found that AND-1 inhibition significantly increased the radiosensitivity of NSCLC cells, likely by regulating the cell cycle and enhancing DNA damage.
Article
Oncology
Romain Tropee, Barbara de la Pena Avalos, Madeline Gough, Cameron Snell, Pascal H. G. Duijf, Eloise Dray
Summary: Chromatin remodeling factor SMARCD3 plays a crucial role in regulating cell proliferation and DNA damage repair. Lower SMARCD3 expression results in decreased cell proliferation rates, cell cycle progression failure, and accumulation of DNA damage, which may contribute to poor survival outcomes in hormone-positive breast cancer patients. SMARCD3 could serve as a potential tumor suppressor and a prognostic biomarker for breast cancer.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Genetics & Heredity
Abigael Cheruiyot, Sharad C. Paudyal, In-Kwon Kim, Melanie Sparks, Tom Ellenberger, Helen Piwnica-Worms, Zhongsheng You
Article
Biochemistry & Molecular Biology
Xiaoqing Chen, In-Kwon Kim, Yuchi Honaker, Sharad C. Paudyal, Won Kyun Koh, Melanie Sparks, Shan Li, Helen Piwnica-Worms, Tom Ellenberger, Zhongsheng You
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Review
Biochemistry & Molecular Biology
Sharad C. Paudyal, Zhongsheng You
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2016)
Article
Cell Biology
Zhiquan Wang, Honglian Zhang, Ji Liu, Abigael Cheruiyot, Jeong-Heon Lee, Tamas Ordog, Zhenkun Lou, Zhongsheng You, Zhiguo Zhang
GENES & DEVELOPMENT
(2016)
Article
Multidisciplinary Sciences
Haoxing Zhang, Hailong Liu, Yali Chen, Xu Yang, Panfei Wang, Tongzheng Liu, Min Deng, Bo Qin, Cristina Correia, Seungbaek Lee, Jungjin Kim, Melanie Sparks, Asha A. Nair, Debra L. Evans, Krishna R. Kalari, Pumin Zhang, Liewei Wang, Zhongsheng You, Scott H. Kaufmann, Zhenkun Lou, Huadong Pei
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Andrew Nickless, Abigael Cheruiyot, Kevin C. Flanagan, David Piwnica-Worms, Sheila A. Stewart, Zhongsheng You
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Sharad C. Paudyal, Shan Li, Hong Yan, Tony Hunter, Zhongsheng You
NUCLEIC ACIDS RESEARCH
(2017)
Article
Multidisciplinary Sciences
Delphine Lemacon, Jessica Jackson, Annabel Quinet, Joshua R. Brickner, Shan Li, Stephanie Yazinski, Zhongsheng You, Grzegorz Ira, Lee Zou, Nima Mosammaparast, Alessandro Vindigni
NATURE COMMUNICATIONS
(2017)
Review
Biochemistry & Molecular Biology
Andrew Nickless, Julie M. Bailis, Zhongsheng You
CELL AND BIOSCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Andrew Nickless, Erin Jackson, Jayne Marasa, Patrick Nugent, Robert W. Mercer, David Piwnica-Worms, Zhongsheng You
Article
Multidisciplinary Sciences
Abigael Cheruiyot, Shan Li, Andrew Nickless, Robyn Roth, James A. J. Fitzpatrick, Zhongsheng You
Article
Genetics & Heredity
Brandon J. Lamarche, Nicole I. Orazio, Brittany Goben, Jill Meisenhelder, Zhongsheng You, Matthew D. Weitzman, Tony Hunter
Article
Biochemistry & Molecular Biology
Shan Li, Zeno Lavagnino, Delphine Lemacon, Lingzhen Kong, Alessandro Ustione, Xuewen Ng, Yuanya Zhang, Yingchun Wang, Bin Zheng, Helen Piwnica-Worms, Alessandro Vindigni, David W. Piston, Zhongsheng You
Article
Cell Biology
Cuige Zhu, Anna Rogers, Karama Asleh, Jennifer Won, Dongxia Gao, Samuel Leung, Shan Li, Kiran R. Vij, Jian Zhu, Jason M. Held, Zhongsheng You, Torsten O. Nielsen, Jieya Shao
Article
Oncology
Abigael Cheruiyot, Shan Li, Sridhar Nonavinkere Srivatsan, Tanzir Ahmed, Yuhao Chen, Delphine S. Lemacon, Ying Li, Zheng Yang, Brian A. Wadugu, Wayne A. Warner, Shondra M. Pruett-Miller, Esther A. Obeng, Daniel C. Link, Dalin He, Fei Xiao, Xiaowei Wang, Julie M. Bailis, Matthew J. Walter, Zhongsheng You
Summary: This study developed a novel NMD reporter system and identified new NMD-promoting factors through a genome-wide CRISPR-Cas9 knockout screen. Mutations in spliceosome genes SF3B1 and U2AF1 were found to attenuate NMD activity, with cells showing increased sensitivity to NMD inhibition. Overexpression of RNase Hi rescued the sensitivity, suggesting a potential therapeutic approach for cancers with spliceosome mutations.