Article
Chemistry, Analytical
Teliang Zhang, Xueliang Wang, Youjun Zeng, Songfeng Huang, Xiaoqi Dai, Weifu Kong, Qian Liu, Jiajie Chen, Junle Qu, Yonghong Shao
Summary: Surface plasmon resonance microscopy (SPRM) is widely used in biological fields for its high spatial resolution and label-free detection. In this study, a home-built SPRM system based on total internal reflection (TIR) was utilized to study the imaging of a single nanoparticle. By using a ring filter and deconvolution algorithm, the parabolic tail of the nanoparticle image was removed, achieving a spatial resolution of 248 nm. Additionally, specific binding between human IgG antigen and goat anti-human IgG antibody was successfully measured using TIR-based SPRM, demonstrating its capability in imaging sparse nanoparticles and monitoring biomolecular interactions.
Article
Polymer Science
J. Alejandro Rivera-Moran, Peter R. Lang
Summary: This paper investigates the influence of noise effects, particle size, and sampling time on total internal reflection microscopy (TIRM) measurement results. It finds that detector shot noise and prolonged sampling times may cause erroneous results in steep parts of the interaction potential, while background noise has negligible effects. Noise does not significantly affect dynamic data, but lengthy sampling times and small probe particle radii can cause issues. Additionally, the study observes that dynamic results may differ from standard hydrodynamic predictions due to partial slip.
Article
Chemistry, Physical
Jing Zeng, Wen Fan, Kangyu Jia, Shuhua Tu, Limin Wu
Summary: A novel retroreflective structural color film (RSCF) based on interference and total internal reflection (TIR) was reported. The film consists of a microscale polymer hemisphere array, displaying different colors under coaxial and noncoaxial illumination and observation. Additionally, an elastic polyurethane-based RSCF can exhibit dynamically changing colors during uniaxial tensile/compressive deformation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Acoustics
Hongfei Zhu, Timothy F. Walsh, Bradley H. Jared, Fabio Semperlotti
Summary: This study discusses the design and application of a nonlocal elastic metasurface for achieving broadband passive vibration isolation in a complex structural assembly. The experimental results confirm the significant vibration isolation capabilities of the nonlocal metasurface, even under non-ideal incidence conditions, extending the validity of the results. Additionally, the study demonstrates that a planar metasurface can be morphed into a curved interface while maintaining full wave control capabilities.
JOURNAL OF SOUND AND VIBRATION
(2022)
Article
Optics
Shijie Sun, Yuanhua Che, Qidong Yu, Yuhang Xie, Tianhang Lian, Xueqing Sun, Xibin Wang, Daming Zhang
Summary: A broadband mode-independent thermo-optic switch based on the total-internal-reflection effect is proposed and experimentally demonstrated on a polymer waveguide platform. By optimizing the geometric parameters of the switch, a mode-independent TO switching function with a large bandwidth and extinction ratio can be realized for different modes. The designed structure can also be cascaded to form a 1 x N switch network for mode-division multiplexing (MDM) systems, improving network flexibility.
Article
Optics
Kevin Affannoukoue, Simon Labouesse, Guillaume Maire, Laurent Gallais, Julien Savatier, Marc Allain, Md Rasedujjaman, Loic Legoff, Jerome Idier, Renaud Poincloux, Florence Pelletier, Christophe Leterrier, Thomas Mangeat, Anne Sentenac
Summary: A benefit of random illumination microscopy (RIM) is that it improves the resolution and linearity of structured illumination microscopy using speckled illumination. In this study, RIM was implemented in the TIRF configuration for imaging biological processes close to the coverslip surface, achieving high-resolution images on fixed samples and dynamic images on live macrophages. The simplicity of the experimental setup and imaging protocol, as well as the robustness of the data processing, make TIRF-RIM a preferred method for super-resolution TIRF imaging.
Article
Multidisciplinary Sciences
Peng Hu, Chongwu Xie, Qianju Song, Ang Chen, Hong Xiang, Dezhuan Han, Jian Zi
Summary: This article elaborates on the formation mechanisms of bound states in the continuum in a photonic-crystal slab and develops a highly efficient solver based on total internal reflections of Bloch waves. A photonic-crystal slab can support bound states in the continuum (BICs) that have infinite lifetimes but are embedded into the continuous spectrum of optical modes in free space. The formation of BICs requires a total internal reflection (TIR) condition at both interfaces between the slab and the free space. The article shows that the TIR of Bloch waves can be directly obtained based on the generalized Fresnel equations proposed. A BIC solver with low computational complexity and fast convergence speed is developed, which can also work efficiently at high frequencies beyond the diffraction limit where multiple radiation channels exist. Two examples of multi-channel BICs are shown and their topological nature in momentum space is also revealed. The concept of the generalized TIR and the TIR-based BIC solver developed offer highly effective approaches for explorations of BICs that could have many potential applications in guided-wave optics and enhanced light-matter interactions.
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemistry & Molecular Biology
Joerg Nikolaus, Kasey Hancock, Maria Tsemperouli, David Baddeley, Erdem Karatekin
Summary: Researchers used a polarization-controlled total internal reflection fluorescence (pTIRF) microscope to monitor the fusion of proteoliposomes with planar lipid bilayers, investigating membrane fusion pore dynamics. By varying excitation polarization angle, they identified a dye-dependent optimal polarization for maximal fluorescence increase upon fusion, aiding event detection and lipid transfer kinetics analysis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Ernesta Buzavaite-Verteliene, Vincentas Maciulis, Justina Anulyte, Tomas Tolenis, Algirdas Baskys, Ieva Plikusiene, Zigmas Balevicius
Summary: In this study, a one-dimensional photonic crystal with an additional TiO2 layer, supporting Bloch surface waves (BSW), was used to enhance the signal sensitivity for protein interaction detection. The results showed that the BSW component of the photonic crystal sample with an additional TiO2 layer had higher sensitivity.
Article
Chemistry, Physical
Jia Meng, Jae Bem You, Gilmar F. Arends, Hao Hao, Xiaoli Tan, Xuehua Zhang
Summary: This study presents a method using a quasi-2D microfluidic device combined with total internal reflection microscopy for in situ observation of phase separation. Diffusion-driven mixing allows for controlled composition of the mixture in the main channel. This approach is demonstrated for observing asphaltene precipitation and beta-alanine crystallization.
EUROPEAN PHYSICAL JOURNAL E
(2021)
Article
Chemistry, Multidisciplinary
Nathaniel E. Sturniolo, Krista Hirsch, Caleb H. Meredith, Beau C. Beshires, Shawn Khanna, Malak S. Rayes, Michael A. Gallegos, Shannon McGee, Bryan Kaehr, Lauren D. Zarzar
Summary: This article presents an experimental investigation and optical modeling of structural coloration produced from total internal reflection interference within 3D microstructures. The iridescence generated for various microgeometries is modeled, examined, and rationalized using ray-tracing simulations, color visualization, and spectral analysis techniques. The observed iridescence is deconstructed into elementary components and linked to ray trajectories, which emanate from the illuminated microstructures. The findings provide a conceptual framework for understanding and tailoring the optical and iridescent properties of microstructured surfaces.
ADVANCED MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Xuanhui Meng, Adar Sonn-Segev, Anne Schumacher, Daniel Cole, Gavin Young, Stephen Thorpe, Robert W. Style, Eric R. Dufresne, Philipp Kukura
Summary: Single particle tracking has broad applications in life and physical sciences, allowing observation and characterization of nano- and microscopic motion. This study demonstrates that micromirror-based total internal reflection dark field microscopy achieves efficient background suppression, with nanometer localization precision and high temporal resolution.
Article
Chemistry, Analytical
Chongqi Zhou, Yang Xu, Xiaonan Zhang, Zhangyan Li, Tian Guan, Yanhong Ji, Yanhong Ji
Summary: A modified weak measurement scheme, rotatory biased weak measurement, is proposed to significantly improve the sensitivity and resolution of the total internal reflection sensor. The method introduces an additional phase in the post-selected procedure and generates an extinction point in the spectrum distribution. The performance of the sensor is analyzed, and an optimal refractive index resolution of 4 x 10(-7) RIU is demonstrated.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Optics
Zetao Fan, Yan Kuai, Xi Tang, Yifan Zhang, Douguo Zhang
Summary: This paper proposes a novel TIRF microscope based on a photonic chip, which can replace conventional objectives or prisms for simplified optical configuration and shadowless illumination.
Article
Chemistry, Analytical
Hongqi Chen, Wanying Xia, Qian Gao, Lun Wang
Summary: A fast and efficient quantitative image analysis method has been developed for the determination of HSO3- concentration, using upconversion nanoparticles and cyanine dye molecules to build a luminescence energy transfer system. The method significantly reduces sample consumption, allows for quick and efficient analysis, and shows a wider linear range, lower detection limit, and higher detection speed compared to the classical luminescence spectrometer.
Article
Cell Biology
Rachel L. Belote, Sanford M. Simon
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon, Charles M. Rice
NATURE COMMUNICATIONS
(2020)
Article
Oncology
James A. Saltsman, William J. Hammond, Nicole J. C. Narayan, David Requena, Helmuth Gehart, Gadi Lalazar, Michael P. LaQuaglia, Hans Clevers, Sanford Simon
Article
Biology
Joan Pulupa, Harriet Prior, Daniel S. Johnson, Sanford M. Simon
Article
Biology
Cristina Olivieri, Caitlin Walker, Adak Karamafrooz, Yingjie Wang, V. S. Manu, Fernando Porcelli, Donald K. Blumenthal, David D. Thomas, David A. Bernlohr, Simon M. Sandford, Susan S. Taylor, Gianluigi Veglia
Summary: The fusion of the dynamic J-domain to PKA-C disrupts the internal allosteric network, causing attenuation of nucleotide/PKI binding cooperativity. This reduced allosteric cooperativity alters specific recognitions and interactions between substrates and regulatory partners, contributing to dysregulation exhibited by PKA-C-DNAJB1.
COMMUNICATIONS BIOLOGY
(2021)
Article
Pathology
Daniel J. Rowan, Saba Yasir, Zongming E. Chen, Taofic Mounajjed, Sibel Erdogan Damgard, Lisa Cummins, Lizhi Zhang, Emma Whitcomb, Vince Falck, Sanford M. Simon, Aatur D. Singhi, Michael S. Torbenson
Summary: Myxoid hepatic adenomas are a rare subtype of hepatic adenomas that predominantly occur in older individuals and have an elevated risk of malignant transformation. They are characterized by the combined inactivation of HNF1A and additional mutations that cluster in the PKA pathway.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Oncology
Gadi Lalazar, David Requena, Lavoisier Ramos-Espiritu, Denise Ng, Patrick D. Bhola, Ype P. de Jong, Ruisi Wang, Nicole J. C. Narayan, Bassem Shebl, Solomon Levin, Eleftherios Michailidis, Mohammad Kabbani, Koen O. A. Vercauteren, Arlene M. Hurley, Benjamin A. Farber, William J. Hammond, James A. Saltsman, Ethan M. Weinberg, J. Fraser Glickman, Barbara A. Lyons, Jessica Ellison, Erik Schadde, Martin Hertl, Jennifer L. Leiting, Mark J. Truty, Rory L. Smoot, Faith Tierney, Tomoaki Kato, Hans-Guido Wendel, Michael P. LaQuaglia, Charles M. Rice, Anthony Letai, Philip Coffino, Michael S. Torbenson, Michael Ortiz, Sanford M. Simon
Summary: Therapeutics informed by genomics have not been effective for fibrolamellar carcinoma (FLC). A functional drug screen identified that inhibitors of TOPO1 and HDAC, along with napabucasin, showed efficacy on patient-derived xenografts (PDX) of FLC. Inhibiting Bcl-xL in combination with these drugs also showed synergy in treating FLC.
Article
Biochemistry & Molecular Biology
Lara Rheinemann, Diane Miller Downhour, Kate Bredbenner, Gaelle Mercenne, Kristen A. Davenport, Phuong Tieu Schmitt, Christina R. Necessary, John McCullough, Anthony P. Schmitt, Sanford M. Simon, Wesley I. Sundquist, Nels C. Elde
Summary: Duplicated and truncated copies of the ESCRT-III factor CHMP3 have been found in New World monkeys and mice, which can block virus budding. These new retroCHMP3 proteins have evolved to reduce interactions with other ESCRT-III factors, providing a new route for inhibiting the release of various viruses.
Article
Cell & Tissue Engineering
Nicole J. C. Narayan, David Requena, Gadi Lalazar, Lavoisier Ramos-Espiritu, Denise Ng, Solomon Levin, Bassem Shebl, Ruisi Wang, William J. Hammond, James A. Saltsman III, Helmuth Gehart, Michael S. Torbenson, Hans Clevers, Michael P. LaQuaglia, Sanford M. Simon
Summary: In this study, patient-derived organoids were successfully developed and used as a model system to recapitulate the histologic morphology, immunohistochemistry, and transcriptome of fibrolamellar carcinoma (FLC). This model system shows promise for improving our understanding of this rare cancer and for drug testing and development.
Article
Oncology
Christoph Neumayer, Denise Ng, Caroline S. Jiang, Adam Qureshi, Gadi Lalazar, Roger Vaughan, Sanford M. Simon
Summary: The fusion gene DNAJB1-PRKACA is found to be a recurrent mutation in fibrolamellar hepatocellular carcinoma (FLC). Continued expression of DNAJB1-PRKACA is essential for tumor growth and its inhibition can lead to cell death. This discovery has implications for investigating other fusion genes in pediatric cancers and advancing precision therapies.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xiao Wang, Sanford M. Simon, Philip Coffino
Summary: This study used single molecule experiments to measure the dwell time of substrates interacting with the AAA+ ATPase unfoldase/translocase ClpX, finding that tail length and composition of the substrates affect substrate retention during futile unfolding. Both polyG and GAr have inhibitory effects on unfolding, but act differently.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Bassem Shebl, Denise Ng, Gadi Lalazar, Carly Rosemore, Tova M. Finkelstein, Rachael D. Migler, Guangrong Zheng, Peiyi Zhang, Caroline S. Jiang, Adam Qureshi, Roger Vaughan, Mark Yarchoan, Ype P. de Jong, Charles M. Rice, Philip Coffino, Michael V. Ortiz, Daohong Zhou, Sanford M. Simon
Summary: Fibrolamellar hepatocellular carcinoma (FLC) is a rare and lethal liver cancer with no effective systemic therapy. Inhibiting the antiapoptotic protein BCL-XL has shown synergy with various systemic therapies for FLC cells in vitro. However, the physiological expression of BCL-XL in platelets has hindered previous attempts to target this vulnerability in other cancers.
Correction
Oncology
Sanford M. Simon
NATURE REVIEWS CANCER
(2023)
Review
Oncology
Sanford M. Simon
Summary: The fight against rare cancers is challenged by issues such as misdiagnosis, lack of information and tools, limited samples, and inadequate funding. However, studying rare cancers has led to important discoveries in cancer biology, such as the identification of tumor suppressor genes. Fibrolamellar hepatocellular carcinoma (FLC) is a rare and difficult-to-diagnose liver cancer that primarily affects young individuals. Through a personal journey, Sanford Simon shares insights into whether FLC is a single cancer type or a collection of similar phenotypes, and its genetic inheritance or somatic mutation origins.
NATURE REVIEWS CANCER
(2023)
Article
Gastroenterology & Hepatology
Amichai Berkovitz, Rachael D. Migler, Adam Qureshi, Carly Rosemore, Michael S. Torbenson, Roger Vaughan, Erin Marcotte, Sanford M. Simon
Summary: Fibrolamellar hepatocellular carcinoma (FLC) is a rare primary liver cancer primarily affecting adolescents and young adults. Female gender is associated with improved survival, while the presence of 10 or more tumors within the liver and metastases at diagnosis are associated with worse survival. This study utilized a patient community-based FLC registry to highlight the importance of rare tumor registries in advancing clinical and scientific discoveries.
HEPATOLOGY COMMUNICATIONS
(2022)
