Review
Engineering, Biomedical
Marcus Ground, Steve Waqanivavalagi, Robert Walker, Paget Milsom, Jillian Cornish
Summary: Tissue engineered heart valves offer a potential alternative to traditional valve prostheses, but predicting their in vivo performance is challenging. Understanding the short and long term immune reactions is crucial for bringing these prostheses to market, and researchers need to utilize a variety of models to fully elucidate the host's impact on the graft.
ACTA BIOMATERIALIA
(2021)
Review
Multidisciplinary Sciences
Nicholas Rogozinski, Apuleyo Yanez, Rahulkumar Bhoi, Moo-Yeal Lee, Huaxiao Yang
Summary: 3D cardiac engineered constructs not only advance cardiac regenerative medicine but also enable more accurate modeling of healthy and diseased cardiac tissues. This review highlights the relationship between different cell types and advanced fabrication methods, shedding light on various pathologies and providing therapeutic potential for damaged myocardium. The future direction is focused on creating specialized and personalized constructs with region-specific microtopography and function.
Article
Engineering, Biomedical
Ramona Hesselbarth, Tilman U. Esser, Kaveh Roshanbinfar, Stefan Schruefer, Dirk W. Schubert, Felix B. Engel
Summary: The integration of CHIR99021 in collagen I hydrogels promotes human-induced pluripotent stem cell-derived cardiomyocyte proliferation, enhancing contractility and calcium flow in engineered cardiac microtissues.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Marcy Martin, Eric K. N. Gahwiler, Melanie Generali, Simon P. Hoerstrup, Maximilian Y. Emmert
Summary: The adult human heart lacks complete regenerative capabilities, posing a challenge for cardiac injury treatment. Pluripotent stem cell technologies and 3D culture systems have revolutionized the field, providing a more accurate model for disease and drug interactions. This study explores the advancements and limitations in stem cell-based cardiac regenerative medicine, including clinical implementation and ongoing trials, as well as the potential of 3D culture systems in generating cardiac organoids for disease modeling. Insights gained from cardiac organoids shed light on cardiac regeneration and have implications for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, Research & Experimental
Jaeyeaon Cho, Hyein Lee, Woongchan Rah, Hyuk Jae Chang, Young-sup Yoon
Summary: This article discusses the use of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in drug screening and disease modeling and introduces the development of three-dimensional cardiac tissue models, including engineered heart tissues and cardiac organoids, analyzing their characteristics, applications, and limitations.
Article
Cardiac & Cardiovascular Systems
Dilip Thomas, Suji Choi, Christina Alamana, Kevin Kit Parker, Joseph C. Wu
Summary: An ensemble of in vitro cardiac tissue models has been developed to aid in the understanding of complex cardiovascular disorders using stem cell differentiation approaches. These models allow for customization and the study of diseased states, with recent progress in the development of various cardiovascular models. However, there are limitations and challenges with current cardiac models that need to be addressed.
CIRCULATION RESEARCH
(2022)
Review
Engineering, Biomedical
Kyeong Seob Hwang, Eun U. Seo, Nakwon Choi, Jongbaeg Kim, Hong Nam Kim
Summary: Viral infections cause damage to various organ systems. Current research mainly focuses on 2D cell culture models and animal models, which have limitations in recapitulating human-specific viral infection pathology. Three-dimensional engineered tissue models have shown potential in replicating infection-associated pathologies.
BIOACTIVE MATERIALS
(2023)
Review
Chemistry, Medicinal
Ying Chen, Ying Wang, Sheng-Chang Luo, Xiang Zheng, Ranjith Kumar Kankala, Shi-Bin Wang, Ai-Zheng Chen
Summary: Body articulation units, also known as body joints, are important for body flexibility in the musculoskeletal system. However, they are prone to various pathological conditions such as arthritis. Existing treatment modalities based on anti-inflammatory and analgesic drugs have limitations in terms of cost and recovery. Engineering strategies, such as fabricating body articulation unit models for tissue regeneration and drug development, have emerged as promising solutions. Challenges and difficulties still exist in the clinical application of these models.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Engineering, Biomedical
Kaveh Roshanbinfar, Maria Kolesnik-Gray, Miriam Angeloni, Stefan Schruefer, Maren Fiedler, Dirk W. Schubert, Fulvia Ferrazzi, Vojislav Krstic, Felix B. Engel
Summary: Cardiac tissue engineering is a promising strategy to prevent heart failure, but several issues remain unsolved. This study develops a biohybrid hydrogel that enhances the beating properties of engineered cardiac tissues and allows for concurrent drug release.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Valeria Vincenza Alvino, Anita C. Thomas, Mohamed T. Ghorbel, Filippo Rapetto, Srinivas A. Narayan, Michael Kilcooley, Dominga Iacobazzi, Michele Carrabba, Marco Fagnano, William Cathery, Elisa Avolio, Massimo Caputo, Paolo Madeddu
Summary: The study evaluated the safety and effectiveness of a matrix graft cellularized with cardiac pericytes in a piglet model of pulmonary artery reconstruction. While the primary goal of supporting the graft's growth was not achieved, the secondary outcomes showed improvements in luminal cellularization, intraparietal vascularization, and elastic remodeling of the recipient artery. The beneficial properties of neonatal cardiac pericytes may have potential applications in bioengineering to reproduce the cellular composition of native arteries.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Medicine, General & Internal
Chang Liu, Xing Feng, Guoping Li, Priyanka Gokulnath, Junjie Xiao
Summary: Human pluripotent stem cell (hPSC) technology provides abundant opportunities for disease modeling. Cardiomyocytes derived from hPSCs are efficient tools for studying cardiac diseases, drug screening, and pathological mechanisms. This review discusses the advantages and limitations of the 2D hPSC-cardiomyocyte system and introduces recent developments in 3D culture platforms derived from hPSCs.
Article
Cardiac & Cardiovascular Systems
Willem J. de Lange, Emily T. Farrell, Caroline R. Kreitzer, Derek R. Jacobs, Di Lang, Alexey Glukhov, J. Carter Ralphe
Summary: This study provides a detailed assessment of engineered human cardiac tissue constructs (hiPSC-ECTs) formed by coculturing hiPSC-CMs and cardiac fibroblasts in a 3D fibrin matrix. The hiPSC-ECTs show improved physiological responsiveness, develop a t-tubular system, and exhibit calcium-handling and contractile kinetics similar to human myocardium. The study emphasizes the suitability of 3D-engineered human cardiac tissues as a model for studying cardiac function and provides data to aid in selecting the most appropriate configuration based on the research question.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Vasco Sampaio-Pinto, Jasmijn Janssen, Nino Chirico, Margarida Serra, Paula M. Alves, Pieter A. Doevendans, Ilja K. Voets, Joost P. G. Sluijter, Linda W. van Laake, Alain van Mil
Summary: Heart failure affects over 26 million patients worldwide, and cardiac tissue-engineered constructs are being explored as a potential solution. Efficient preservation methods are crucial for the availability of these constructs in clinical settings. Current research suggests that vitrification could be a promising approach for long-term cryopreservation, while short-term preservation can be achieved using protective additives at normothermic or hypothermic temperatures.
ADVANCED MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Vittorio Picchio, Erica Floris, Yuriy Derevyanchuk, Claudia Cozzolino, Elisa Messina, Francesca Pagano, Isotta Chimenti, Roberto Gaetani
Summary: Ex vivo modelling systems are important in cardiovascular research for reducing lab animal use and advancing personalized medicine. Integrating multiple cell types in complex setups simulates the intercellular communication of the microenvironment. 3D culture systems enhance the physiological significance of these models. Various multicellular 3D models, such as microtissues, bioprinted constructs, engineered tissues, and organs-on-chip, have been used to study cardiac fibrosis, leading to important discoveries and potential therapeutic molecules. Future developments will further enhance the translational impact of these models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Ferdous B. Finklea, Yuan Tian, Petra Kerscher, Wen J. Seeto, Morgan E. Ellis, Elizabeth A. Lipke
Summary: The study demonstrates successful production of functional cardiac tissue microspheres through direct differentiation of hydrogel encapsulated hiPSCs, with high reproducibility and cell viability. These microspheres support efficient cardiac differentiation and have potential applications in biomanufacturing, drug screening, and regenerative therapies.
Article
Multidisciplinary Sciences
Fang Liu, David K. Jones, Willem J. de Lange, Gail A. Robertson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Review
Cardiac & Cardiovascular Systems
Alison J. Kriegel, Melanie Gartz, Muhammad Z. Afzal, Willem J. de Lange, J. Carter Ralphe, Jennifer L. Strande
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2017)
Article
Physiology
Emily T. Farrell, Adrian C. Grimes, Willem J. de Lange, Annie E. Armstrong, J. Carter Ralphe
FRONTIERS IN PHYSIOLOGY
(2017)
Article
Cardiac & Cardiovascular Systems
Dan F. Smelter, Willem J. de Lange, Wenxuan Cai, Ying Ge, J. Carter Ralphe
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2018)
Article
Hematology
Henry L. Keen, Carmen M. Halabi, Andreas M. Beyer, Willem J. de Lange, Xuebo Liu, Nobuyo Maeda, Frank M. Faraci, Thomas L. Casavant, Curt D. Sigmund
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2010)
Meeting Abstract
Biophysics
Matthew R. Locher, Willem J. de lange, Laura F. Hegge, Adrian C. Grimes, John C. Ralphe, Richard L. Moss
BIOPHYSICAL JOURNAL
(2012)
Article
Cell Biology
Justin L. Grobe, Connie L. Grobe, Terry G. Beltz, Scott G. Westphal, Donald A. Morgan, Di Xu, Willem J. de Lange, Huiping Li, Koji Sakai, Daniel R. Thedens, Lisa A. Cassis, Kamal Rahmouni, Allyn L. Mark, Alan Kim Johnson, Curt D. Sigmund
Article
Cell Biology
Christopher J. Pelham, Pimonrat Ketsawatsomkron, Severine Groh, Justin L. Grobe, Willem J. de Lange, Stella-Rita C. Ibeawuchi, Henry L. Keen, Eric T. Weatherford, Frank M. Faraci, Curt D. Sigmund
Article
Cardiac & Cardiovascular Systems
W. J. de Lange, L. F. Hegge, A. C. Grimes, C. W. Tong, T. M. Brost, R. L. Moss, J. C. Ralphe
CIRCULATION RESEARCH
(2011)
Article
Peripheral Vascular Disease
Andreas M. Beyer, Gary L. Baumbach, Carmen M. Halabi, Mary L. Modrick, Cynthia M. Lynch, Thomas D. Gerhold, Shams M. Ghoneim, Willem J. de Lange, Henry L. Keen, Yau-Sheng Tsai, Nobuyo Maeda, Curt D. Sigmund, Frank M. Faraci
Article
Biochemistry & Molecular Biology
Kunil K. Raval, Ran Tao, Brent E. White, Willem J. De lange, Chad H. Koonce, Junying Yu, Priya S. Kishnani, James A. Thomson, Deane F. Mosher, John C. Ralphe, Timothy J. Kamp
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Physiology
Willem J. de Lange, Adrian C. Grimes, Laura F. Hegge, J. Carter Ralphe
JOURNAL OF GENERAL PHYSIOLOGY
(2013)
Article
Physiology
Willem J. De lange, Adrian C. Grimes, Laura F. Hegge, Alexander M. Spring, Taylor M. Brost, J. Carter Ralphe
JOURNAL OF GENERAL PHYSIOLOGY
(2013)
Article
Physiology
Gregory P. Barton, Willem J. de Lange, John C. Ralphe, Judd Aiken, Gary Diffee
PHYSIOLOGICAL REPORTS
(2017)