期刊
TRENDS IN BIOTECHNOLOGY
卷 26, 期 2, 页码 82-89出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2007.11.004
关键词
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Lipid membranes are versatile and convenient models for the study of properties of natural cell membranes. In particular, surface-supported membranes have attracted considerable attention because the whole range of surface-sensitive techniques, including interface-sensitive electrochemical measurements, can be used with them. Here we describe recent advances and current directions in the development of nano- and microporous substrates for electrochemical characterization of membrane protein-containing lipid bilayers. Improved techniques for lipid membrane self-assembly and membrane protein incorporation on these substrates have led to great improvements in measurement sensitivity, membrane stability and packaging. These advances suggest that nanopore-spanning membranes are leading contenders for a breakthrough in membrane protein screening and biosensing applications.
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