Article
Biochemistry & Molecular Biology
Jessica M. Warren, Thalia Salinas-Giege, Deborah A. Triant, Douglas R. Taylor, Laurence Drouard, Daniel B. Sloan
Summary: Research on mitochondrial genomes of Silene plants reveals the process of tRNA gene loss and replacement, as well as the import of nuclear-encoded tRNAs. Even in systems with recent mitochondrial tRNA gene loss, nuclear-encoded counterparts may likely replace mitochondrial tRNAs, providing insight into the evolutionary history of plant mitochondrial genomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Editorial Material
Multidisciplinary Sciences
Cecilia Giulivi, Kezhong Zhang, Hirofumi Arakawa
Summary: In the past decade, there has been a growing recognition of the significant role mitochondria play in various cellular processes, including energy production, immune response, and signal transmission. It is now known that mitochondrial dysfunction is implicated in many diseases, such as primary mitochondrial diseases caused by mutations in mitochondrial genes, secondary mitochondrial diseases caused by mutations in non-mitochondrial genes essential for mitochondrial function, and complex diseases associated with mitochondrial dysfunction. Moreover, evidence suggests that mitochondrial dysfunction often precedes other pathological signs, with its manifestation influenced by genetics, environment, and lifestyle.
SCIENTIFIC REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Bibekananda Kar, Santiago R. Castillo, Ankit Sabharwal, Karl J. Clark, Stephen C. Ekker
Summary: Mitochondria are crucial organelles involved in energy generation and cell functionality, as well as producing important signaling molecules. They can vary between cells, tissues, and organs, and undergo changes due to disease, aging, and environmental factors. Human mitochondrial DNA can have single nucleotide variants that are linked to life-threatening diseases. Mitochondrial DNA base editing tools have proven useful in creating disease models and offer potential for personalized gene therapies targeting mtDNA-based disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Clinical Neurology
Yi Shiau Ng, Laurence A. Bindoff, Grainne S. Gorman, Thomas Klopstock, Cornelia Kornblum, Michelangelo Mancuso, Robert McFarland, Carolyn M. Sue, Anu Suomalainen, Robert W. Taylor, David R. Thorburn, Doug M. Turnbull
Summary: In the past five years, significant progress has been made in understanding, diagnosing, and treating mitochondrial diseases. National cohorts and international collaborations have expanded knowledge of the clinical phenotypes and natural history, high-throughput sequencing has altered diagnostic approaches, and efforts are ongoing to find better treatments including drug repurposing and gene therapies. Reproductive technologies also offer opportunities to prevent DNA-related mitochondrial disease transmission.
Review
Chemistry, Multidisciplinary
Liying Meng, Guanzhao Wu
Summary: Mitochondrial disorders are observed in various human diseases and therapeutic methods for mitochondrial disorders are limited. This review focuses on the latest advances in developing bioactive compounds for treating mitochondrial disease, aiming to provide a broader perspective of fundamental studies that have been carried out to evaluate the effects of small molecules in regulating mitochondrial function.
Review
Genetics & Heredity
Suleva Povea-Cabello, Marina Villanueva-Paz, Juan M. Suarez-Rivero, Monica alvarez-Cordoba, Irene Villalon-Garcia, Marta Talaveron-Rey, Alejandra Suarez-Carrillo, Manuel Munuera-Cabeza, Jose A. Sanchez-Alcazar
Summary: Mitochondrial diseases are rare genetic disorders caused by mutations in nuclear or mitochondrial DNA. These diseases mainly affect energy-demanding organs like the brain and skeletal muscle. Researchers are exploring novel models such as induced pluripotent stem cells to study these diseases and develop new therapeutic options.
FRONTIERS IN GENETICS
(2021)
Review
Pharmacology & Pharmacy
Chayodom Maneechote, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Doxorubicin is an effective chemotherapy drug, but its cardiotoxicity limits its effectiveness. Recent research has shown that excessive mitochondrial fission and impaired fusion contribute to this cardiotoxicity. However, using inhibitors of mitochondrial fission or promoters of fusion can protect the heart against the toxicity of doxorubicin.
Review
Cell Biology
Mario G. Pavez-Giani, Lukas Cyganek
Summary: This review summarizes recent advances in iPSC-based disease modeling of mitochondrial cardiomyopathies and explores the patho-mechanistic insights as well as new therapeutic approaches. Researchers have used iPSC technology to recapitulate major characteristics of mitochondrial cardiomyopathies, providing a powerful platform for drug development and testing of new therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lin Yan, Zhenzhen Hou, Jinnan Ma, Hongmei Wang, Jie Gao, Chenjuan Zeng, Qin Chen, Bisong Yue, Xiuyue Zhang
Summary: The complete mitochondrial genome of Episymploce splendens was determined and annotated in this study. It showed distinct features compared to other insect mitochondrial genomes, including the absence of two tRNAs and a long non-coding spacer sequence. Additionally, two Numts transferred to the nucleus were discovered in this species, serving as molecular fossils for insect phylogenetic relationship inference.
Review
Chemistry, Multidisciplinary
Sung-Eun Choi, Harrison Khoo, Soojung Claire Hur
Summary: This article introduces the physical principles and design trends of microscale electroporation devices and discusses their applications in drug development and cell analysis, among other areas.
Review
Chemistry, Multidisciplinary
Xinyue Tan, Huadong Wang
Summary: This review provides an overview of recent advancements in borenium-catalyzed reactions, with a focus on catalyst synthesis, methodology development, and mechanistic insight.
CHEMICAL SOCIETY REVIEWS
(2022)
Review
Chemistry, Multidisciplinary
Dong Qiu, Chuanhui Gong, SiShuang Wang, Miao Zhang, Chao Yang, Xianfu Wang, Jie Xiong
Summary: The emergence of superconductivity in 2D materials has attracted attention due to their unique physical properties. Researchers have made efforts to explore mechanisms behind the unexpected superconductivity phenomena by adjusting different physical parameters. Different types of 2D superconductivity are introduced in the article, along with a perspective towards the future of this field.
ADVANCED MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Jorge Diaz-Rullo, Jose Eduardo Gonzalez-Pastor
Summary: In this study, we developed a novel bioinformatic strategy to predict Q-genes and found that these genes are widely enriched in cellular functions, especially in biofilm formation and virulence in bacteria, particularly in human pathogens. Experimental validation showed that altering the level of tRNA Q-modification significantly affected these physiological processes in different model bacteria, representing the first report of a general mechanism controlling biofilm formation and virulence in Gram-positive and Gram-negative bacteria. Additionally, we propose that changes in Q availability in a microbiome would impact its functionality. These findings open new avenues for the control of bacterial infections and biofilm formation by inhibiting tRNA Q-modification.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Naintara Jain, Ridhima Gomkale, Olaf Bernhard, Peter Rehling, Luis Daniel Cruz-Zaragoza
Summary: Mitochondria play a crucial role in cellular energy production and metabolism, and errors in mitochondrial protein import can lead to metabolic disorders. This study establishes a fluorescence-based import assay to analyze protein import into mitochondria, offering the advantage of quantifying import with high sensitivity. The assay can be adapted to a screening-compatible format and used to monitor the assembly of the F1F0 ATP synthase in purified mitochondria.
Article
Multidisciplinary Sciences
F. X. Reymond Sutandy, Ines Goessner, Georg Tascher, Christian Muench
Summary: This study demonstrates that cytosol-mitochondrial reactive oxygen species (mtROS) and accumulation of mitochondrial protein precursors in the cytosol (c-mtProt) play a critical role in initiating the mitochondrial unfolded protein response (UPRmt). The findings reveal a link between mitochondrial and cytosolic proteostasis and provide molecular insight into UPRmt signaling in human cells.
Article
Biochemistry & Molecular Biology
Valerie Cognat, Geoffrey Morelle, Cyrille Megel, Stephanie Lalande, Jean Molinier, Timothee Vincent, Ian Small, Anne-Marie Duchene, Laurence Marechal-Drouard
NUCLEIC ACIDS RESEARCH
(2017)
Article
Plant Sciences
Monika W. Murcha, Szymon Kubiszewski-Jakubiak, Pedro F. Teixeira, Irene L. Guegel, Beata Kmiec, Reena Narsai, Aneta Ivanova, Cyrille Megel, Annette Schock, Sabrina Kraus, Oliver Berkowitz, Elzbieta Glaser, Katrin Philippar, Laurence Marechal-Drouard, Juergen Soll, James Whelan
Article
Biochemistry & Molecular Biology
Thalia Salinas-Giege, Marina Cavaiuolo, Valerie Cognat, Elodie Ubrig, Claire Remacle, Anne-Marie Duchene, Olivier Vallon, Laurence Marechal-Drouard
NUCLEIC ACIDS RESEARCH
(2017)
Article
Plant Sciences
Timothee Vincent, Audrey Vingadassalon, Elodie Ubrig, Kevin Azeredo, Ola Srour, Valerie Cognat, Stefanie Graindorge, Thalia Salinas, Laurence Marechal-Drouard, Anne-Marie Duchene
Article
Biochemistry & Molecular Biology
Cyrille Megel, Guillaume Hummel, Stephanie Lalande, Elodie Ubrig, Valerie Cognat, Geoffrey Morelle, Thalia Salinas-Giege, Anne-Marie Duchene, Laurence Marechal-Drouard
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Jean-Stephane Varre, Nunzio D'Agostino, Pascal Touzet, Sophie Gallina, Rachele Tamburino, Concita Cantarella, Elodie Ubrig, Teodoro Cardi, Laurence Drouard, Jose Manuel Gualberto, Nunzia Scotti
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Sean A. Montgomery, Yasuhiro Tanizawa, Bence Galik, Nan Wang, Tasuku Ito, Takako Mochizuki, Svetlana Akimcheva, John L. Bowman, Valerie Cognat, Laurence Marechal-Drouard, Heinz Ekker, Syuan-Fei Hong, Takayuki Kohchi, Shih-Shun Lin, Li-Yu Daisy Liu, Yasukazu Nakamura, Lia R. Valeeva, Eugene Shakirov, Dorothy E. Shippen, Wei-Lun Wei, Masaru Yagura, Shohei Yamaoka, Katsuyuki T. Yamato, Chang Liu, Frederic Berger
Article
Biochemistry & Molecular Biology
Stephanie Lalande, Remy Merret, Thalia Salinas-Giege, Laurence Drouard
Article
Cell Biology
Mickaele Hemono, Elodie Ubrig, Kevin Azeredo, Thalia Salinas-Giege, Laurence Drouard, Anne-Marie Duchene
Article
Biochemistry & Molecular Biology
Jessica M. Warren, Thalia Salinas-Giege, Guillaume Hummel, Nicole L. Coots, Joshua M. Svendsen, Kristen C. Brown, Laurence Drouard, Daniel B. Sloan
Summary: Differences in tRNA expression play a crucial role in biological processes, but measuring abundance and detecting modifications remains challenging. Researchers modified standard RNA-seq methods to sequence tRNAs from flowering plants and produced a modification index, revealing insights into tRNA modifications.
Article
Biochemistry & Molecular Biology
Guillaume Hummel, Alexandre Berr, Stefanie Graindorge, Valerie Cognat, Elodie Ubrig, David Pflieger, Jean Molinier, Laurence Drouard
NUCLEIC ACIDS RESEARCH
(2020)
Article
Plant Sciences
Thalia Salinas-Giege, Elodie Ubrig, Laurence Drouard
Summary: Mitochondria have evolved considerably since originating from a single endosymbiosis event, acquiring unique traits in different eukaryotic kingdoms. The freshwater alga Cyanophora paradoxa serves as an exciting model for studying the evolution of mitochondrial gene expression, showing that tRNAs can function in mRNA processing in a non-conventional manner. The tRNA-like elements found in the 3'-terminals of mitochondrial mRNAs in land plants are proposed to originate from true tRNAs found in the mitochondria of basal photosynthetic glaucophyte.
Article
Biochemistry & Molecular Biology
Elizabeth Ramos-Morales, Efil Bayam, Jordi Del-Pozo-Rodriguez, Thalia Salinas-Giege, Martin Marek, Peggy Tilly, Philippe Wolff, Edouard Troesch, Eric Ennifar, Laurence Drouard, Juliette D. Godin, Christophe Romier
Summary: This study provides insights into the molecular mechanisms of tRNA deamination by ADAT2 and the importance of the ADAT3 N-terminal domain in tRNA binding and deamination. The rotation of the N-terminal domain with respect to the catalytic domain plays a crucial role in presenting and positioning the tRNA anticodon-stem-loop correctly in the ADAT2 active site. A founder mutation in the ADAT3 N-terminal domain, causing intellectual disability, is shown to hinder optimal presentation of the tRNA anticodon-stem-loop to ADAT2.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Florent Waltz, Thalia Salinas-Giege, Robert Englmeier, Herrade Meichel, Heddy Soufari, Lauriane Kuhn, Stefan Pfeffer, Friedrich Forster, Benjamin D. Engel, Philippe Giege, Laurence Drouard, Yaser Hashem
Summary: The study explores the structural and species-specific adaptations of the mitochondrial ribosome in Chlamydomonas reinhardtii, shedding light on the extreme diversity of mitoribosomes and their membrane tethering strategies.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jessica M. Warren, Thalia Salinas-Giege, Deborah A. Triant, Douglas R. Taylor, Laurence Drouard, Daniel B. Sloan
Summary: Research on mitochondrial genomes of Silene plants reveals the process of tRNA gene loss and replacement, as well as the import of nuclear-encoded tRNAs. Even in systems with recent mitochondrial tRNA gene loss, nuclear-encoded counterparts may likely replace mitochondrial tRNAs, providing insight into the evolutionary history of plant mitochondrial genomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)