4.7 Article

Observation of the scale of patchy stomatal behavior in leaves of Quercus crispula using an Imaging-PAM chlorophyll fluorometer

期刊

TREE PHYSIOLOGY
卷 32, 期 7, 页码 839-846

出版社

OXFORD UNIV PRESS
DOI: 10.1093/treephys/tps053

关键词

chlorophyll fluorescence imaging; heterobaric leaf; midday depression of photosynthesis; patchy stomatal closure

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资金

  1. JSPS (Japan Society for the Promotion Science) [23580207]
  2. Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)
  3. Grants-in-Aid for Scientific Research [23580207] Funding Source: KAKEN

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Patchy stomatal closure occurs in plants with heterobaric leaves, in which vertical extensions of bundle sheath cells delimit the mesophyll and restrict the diffusion of CO2. The scale of patchy stomatal behavior was investigated in this study. The distribution of PSII quantum yield (Phi(II)) obtained from chlorophyll fluorescence images was used to evaluate the scale of stomatal patchiness and its relationship with leaf photosynthesis in the sun leaves of 2-year-old saplings of Quercus crispula Blume. Fluorescent patches were observed only during the day with low stomatal conductance. Comparison of numerical simulation of leaf gas exchange and chlorophyll fluorescence images showed that heterogeneous distribution of electron transport rate through PSII (J) was observed following stomatal closure with a bimodal manner under both natural and saturated photosynthetic photon flux densities. Thus, fluorescence patterns can be interpreted in terms of patchy stomatal closure. The mapping of J from chlorophyll fluorescence images showed that the scale of stomatal patchiness was approximately 2.5-fold larger than that of anatomical patches (lamina areas bounded by bundle sheath extensions within lamina). Our results suggest the spatial scale of stomatal patches in Q. crispula leaves.

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