Article
Plant Sciences
Chang-Min Lee, Hyun-Su Park, Man-Kee Baek, O-Young Jeong, Jeonghwan Seo, Suk-Man Kim
Summary: Climate change has threatened the stability of crop cultivation and production due to global warming. Pre-harvest sprouting (PHS) poses a threat to crops, especially staple foods like rice, resulting in reduced yield and quality. In this study, QTL analysis was performed to identify genetic markers associated with PHS resistance using RILs populations derived from weedy rice in Korea. The analysis revealed two stable QTLs, qPH7 and qPH2, on chromosomes 7 and 2 respectively, explaining around 38% of the phenotypic variation. Fine mapping of qPH7 identified a specific region on chromosome 7 associated with PHS, and the upregulated expression of the Os07g0584366 gene was found in the resistant donor. Japonica lines with QTLs related to PHS resistance were developed to improve PHS characteristics and facilitate marker-assisted backcrossing with susceptible cultivars.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Mercedeh Movassagh, Steven J. Schiff, Joseph N. Paulson
Summary: Recent advancements in genomics have allowed researchers to explore the interplay between genetic mutations and microbiome data, providing insights into disease progression and individual responses to treatment interventions.
Article
Agronomy
Modester Kachapila, Yuki Horiuchi, Hidetaka Nagasawa, Noe Michihata, Toru Yoshida, Yuta Kato, Paul C. Bethke, Kiyoaki Kato, Masahiko Mori
Summary: By studying the progeny of a cross between two bean cultivars, four genetic loci that affect epicotyl length were found, and the chromosomal location of one of these loci was narrowed down using molecular markers, which can be used for selecting bean cultivars with longer epicotyls that are suitable for mechanical weeding and harvesting.
Article
Biotechnology & Applied Microbiology
Maya Hiltpold, Naveen Kumar Kadri, Fredi Janett, Ulrich Witschi, Fritz Schmitz-Hsu, Hubert Pausch
Summary: The study identified novel recessive QTL for male fertility in Brown Swiss cattle, with one QTL associated with sperm head shape anomalies. Fine-mapping approaches and whole-genome sequencing revealed candidate causal variants linked to these QTL, suggesting that non-coding variants may play a significant role in bull fertility variation.
Article
Plant Sciences
Hiroyuki Kakui, Eriko Tsurisaki, Rei Shibata, Yoshinari Moriguchi
Summary: Genetic variation, male strobilus weight, and pollen size are the main factors affecting the pollen number in Japanese cedar. The pollen numbers vary significantly among different clones, with environmental conditions having a less significant impact. Understanding these factors will aid in breeding strategies to combat pollinosis.
Article
Plant Sciences
Jia Wang, Lin Mao, Zhaoqiong Zeng, Xiaobo Yu, Jianqiu Lian, Jun Feng, Wenying Yang, Jiangang An, Haiying Wu, Mingrong Zhang, Liezhao Liu
Summary: This study utilized QTL mapping, QTL-seq, and RNA sequencing to identify genes controlling protein content in soybean using the Nanxiadou 25 variety. Major QTLs on chromosome 20 were repeatedly detected across multiple environments, indicating potential candidate genes. Through gene expression analysis and clone sequencing, different SNPs and indels were identified as potentially responsible for changes in protein content.
Article
Plant Sciences
Carmen Santos, Davide Coelho Martins, Maria Jose Gonzalez-Bernal, Diego Rubiales, Maria Carlota Vaz Patto
Summary: In this study, a combination of phenotyping, gene expression, and linkage mapping was used to prioritize four candidate genes relevant for disease-resistance precision breeding in Lathyrus cicera. These genes are involved in adaptation to biotic stress, cellular and organelle homeostasis, and proteins directly involved in plant defense.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Siti Hazirah Zolkafli, Ngoot-Chin Ting, Nik Shazana Nik Mohd Sanusi, Maizura Ithnin, Sean Mayes, Festo Massawe, Ravigadevi Sambanthamurthi, Ismanizan Ismail, Mohd Isa Zainol Abidin, Siti Habsah Roowi, Yang Ping Lee, Nurul Fatiha Farhana Hanafi, Rajinder Singh
Summary: By analyzing QTLs associated with YCs in oil palm, this study revealed potential genetic factors influencing yield. A comparison of QTLs from different genetic backgrounds identified common chromosomes that may play a role in regulating YCs. The use of genome data also facilitated the discovery of candidate genes related to oil palm yield.
Article
Genetics & Heredity
Nagenahalli Dharmegowda Rathan, Deepmala Sehgal, Karthikeyan Thiyagarajan, Ravi Singh, Anju-Mahendru Singh, Velu Govindan
Summary: The study on nutritionally enhanced wheat identified new genomic regions controlling grain iron and zinc concentrations, leading to the discovery of pleiotropic QTL that could be used in wheat biofortification breeding to address micronutrient deficiency among wheat consumers. The in silico analysis revealed candidate genes involved in transporting small peptides and minerals as well as catalyzing phosphorylation processes, providing insights into the mechanisms of mineral uptake in wheat.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Harsh Raman, Nawar Shamaya, Ramethaa Pirathiban, Brett McVittie, Rosy Raman, Brian Cullis, Andrew Easton
Summary: Canola plants have reduced crop yield and oil content under water-deficit conditions, especially during reproductive stages. This study analysed the genetic factors affecting seed yield and related traits in canola under well-watered and water-deficit conditions. The analysis revealed multiple genomic regions associated with flowering time, plant height, and seed yield under different water regimes. The study identified specific QTLs that were stable across environments and showed potential for improving water-use efficiency in canola breeding programs.
Article
Plant Sciences
Qing Meng, Zhiyuan Liu, Chunda Feng, Helong Zhang, Zhaosheng Xu, Xiaowu Wang, Jian Wu, Hongbing She, Wei Qian
Summary: In this study, a major quantitative trait locus (QTL) related to early bolting in spinach was identified, and two candidate genes associated with flowering regulatory pathways were proposed. This research provides a basis for understanding the molecular mechanisms of bolting in spinach.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell & Tissue Engineering
Katya L. Mack, Heather E. Talbott, Michelle F. Griffin, Jennifer B. L. Parker, Nicholas J. Guardino, Amanda F. Spielman, Michael F. Davitt, Shamik Mascharak, Mauricio Downer, Annah Morgan, Caleb Valencia, Deena Akras, Mark J. Berger, Derrick C. Wan, Hunter B. Fraser, Michael T. Longaker
Summary: In adult mammals, skin wounds typically heal through scarring. However, the MRL mice have the ability to regenerate ear punch wounds. The researchers identified the molecular basis for this regeneration by analyzing gene expression in hybrid crosses between MRL and non-regenerating mice. They found that regulatory differences in fibroblasts were associated with wound-healing pathways and treatment with one of these proteins accelerated wound repair and induced regeneration in fibrotic wounds. The study provides insights into the molecular drivers of regeneration with potential clinical implications.
Article
Biochemical Research Methods
Jing Chen, Lindsey J. Leach, Zewei Luo
Summary: Mapping quantitative trait loci (QTL) in autotetraploid species is a timely and challenging task. Two papers by Wu and his colleagues proposed statistical methods for QTL mapping in these species, but have been criticized for fundamental conceptual errors from statistical and genetic perspectives.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Plant Sciences
Jianqin Xu, Xiaoxin Qin, Huaqing Zhu, Fanjun Chen, Xiuyi Fu, Futong Yu
Summary: Iron (Fe) deficiency is a major stress in crop production. This study used maize inbred lines to investigate the physiological mechanism underlying Fe deficiency tolerance and identified several candidate genes associated with Fe deficiency tolerance. The findings provide insights into the response to Fe deficiency stress in maize.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Mohammed Abdelaziz Sayed, Saad Mohamed Nassar, Ehab Soudi Moustafa, Mohamed Tharwat Said, Andreas Boerner, Alhosein Hamada
Summary: This study identified important genomic regions associated with grain weight and salinity tolerance in barley, as well as pinpointed specific markers linked to vernalization, dwarfing, and dehydrin genes. The evaluation of different barley genotypes revealed that wild and elite barley varieties possess valuable alleles that can enhance salinity tolerance and improve yield under salt stress conditions.