Review
Pharmacology & Pharmacy
Long Bai, Gang Tao, Maogeng Feng, Yuping Xie, Shuyu Cai, Shuanglin Peng, Jingang Xiao
Summary: With the advancement in understanding bone regeneration mechanisms and development of bone tissue engineering, a variety of scaffold carrier materials called hydrogels have emerged. Hydrogels are biocompatible, have unique swelling properties, and are relatively easy to fabricate, making them increasingly popular in bone regeneration and tissue engineering. Hydrogel drug delivery systems consist of cells, cytokines, an extracellular matrix, and small molecule nucleotides, each with different properties based on their chemical or physical cross-linking. This paper summarizes recent research in the field of bone regeneration using hydrogels as delivery carriers, discusses their application in bone defect diseases and their mechanisms, and explores future research directions of hydrogel drug delivery systems in bone tissue engineering.
Article
Biotechnology & Applied Microbiology
Yue Ke, Yu Ye, Jintao Wu, Yanxia Ma, Yuxin Fang, Fei Jiang, Jinhua Yu
Summary: A phosphoserine-loaded chitosan membrane was synthesized to improve the osteogenic effect of chitosan membranes. The incorporation of phosphoserine into chitosan membranes was confirmed and the phosphoserine-chitosan membranes significantly stimulated bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Izumi Asahina, Hideaki Kagami, Hideki Agata, Masaki J. Honda, Yoshinori Sumita, Minoru Inoue, Tokiko Nagamura-Inoue, Arinobu Tojo
Summary: This study evaluated the safety and efficacy of bone tissue engineering using BMSCs in patients with severe atrophic alveolar bone. The results showed good long-term stability and safety of the regenerated bone, suggesting potential application in clinical practice. However, individual variations were observed, highlighting the need for further studies with larger sample sizes to confirm the efficacy of BMSCs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Zihang Ma, Xiaoying Guo, Jun Zhang, Qifeng Jiang, Wuying Liang, Wenxin Meng, Shuaijiang Chen, Yufan Zhu, Cundong Ye, Kun Jia
Summary: This study aimed to evaluate the therapeutic effect of combining canine bone marrow mesenchymal stem cells (cBMSCs) with gelatin-nano-hydroxyapatite (Gel-nHAP) on bone defect diseases in dogs. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In animal experiments, significant cortical bone growth was observed in the Gel-nHAP group at week 8 and in the cBMSCs-Gel-nHAP group at week 4.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Nadja Engel, Carsten Fechner, Annika Voges, Robert Ott, Jan Stenzel, Stefan Siewert, Carina Bergner, Valeria Khaimov, Jan Liese, Klaus-Peter Schmitz, Bernd Joachim Krause, Bernhard Frerich
Summary: Efficient and homogeneous seeding of stem cells in bone substitute scaffolds was achieved using a newly designed bioreactor with a conical diffuser geometry. Optimization of flow rates, cell density, and perfusion properties improved the distribution homogeneity throughout the scaffold.
SCIENTIFIC REPORTS
(2021)
Retraction
Biochemistry & Molecular Biology
Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer, Christophe Poulet, Benoit Hennuy, Lukas Sommer, Olga Shakhova, Bernard Rogister
Summary: A correction to this paper has been published.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Polymer Science
Chong Teng, Zhicheng Tong, Qiulin He, Huangrong Zhu, Lu Wang, Xianzhu Zhang, Wei Wei
Summary: In this study, biocompatible hydrogel microspheres were fabricated to promote the osteogenic differentiation of stem cells and enhance bone regeneration. The results from in vivo experiments demonstrated the superiority of the BMSC/HM system in inducing bone regeneration. This study provides a simple and effective research idea for bone defect repair, and the optimization of hydrogel microspheres holds promise for future tissue engineering applications.
Review
Biochemistry & Molecular Biology
Shuntaro Yamada, Philipp Niklas Ockermann, Thomas Schwarz, Kamal Mustafa, Jan Hansmann
Summary: This review primarily focuses on the biophysical environment in bone and its application in 2D and 3D cell culture, including the impact of mechanical stimuli on bone cell growth and fate, as well as the use of dynamic cell culture platforms in bone tissue engineering.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Giusi Alberti, Eleonora Russo, Simona Corrao, Rita Anzalone, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: Extracellular vesicles (EVs) are one of the main mechanisms of communication between cells and the surrounding tissue or at distance. Recent studies have revealed the molecular composition and clinical indications of EVs derived from adult mesenchymal stromal cells (MSCs). These EVs maintain some of the properties of their parent cells and have important implications for cell-free therapies.
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shiau-Ting Shiu, Wei-Fang Lee, Sheng-Min Chen, Liu-Ting Hao, Yuan-Ting Hung, Pin-Chuang Lai, Sheng-Wei Feng
Summary: This study evaluated the new bone formation potential of MBCP and Bio-Oss grafting materials with DPSCs and BMSCs in a rabbit calvarial bone defect model. The results demonstrated that autogenous bone is the gold standard, and both DPSCs and BMSCs enhanced the osteoconductive capacities of MBCP and Bio-Oss. Moreover, the efficiency of BMSCs combined with MBCP and Bio-Oss was comparable to that of autogenous bone after 8 weeks of healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Feng Wang, Wenbo Wang, Lingchi Kong, Li Shi, Mengwei Wang, Yimin Chai, Jia Xu, Qinglin Kang
Summary: This study found that ADM2 accelerates bone regeneration by enhancing the osteogenic differentiation and pro-angiogenic potential of BMSCs, possibly through the NF-κB/β-catenin and AKT/β-catenin pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Health Care Sciences & Services
Cheng-Feng Chu, Shih-Hsuan Mao, Victor Bong-Hang Shyu, Chih-Hao Chen, Chien-Tzung Chen
Summary: The study found that allogeneic BMSCs can promote bone regeneration to some extent and gradually match the efficacy of autologous BMSCs after a period of time. In terms of immune response, allogeneic BMSCs caused a weaker immune response in vivo and the bone regeneration effects gradually matched those of autologous BMSCs.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Yongbo Li, Zhe Tan, Jixiang Zhang, Junhan Mu, Han Wu
Summary: In this study, nano-deer bone meal was successfully prepared and found to have a higher Ca/P ratio and enriched beneficial trace elements compared to cow bone. The nano-deer bone meal showed good biocompatibility and met the standards of medical biomaterials. Cell function studies demonstrated its excellent performance in the proliferation and differentiation of bone marrow mesenchymal stem cells. These findings suggest the potential use of nano-deer bone meal as an active nanomaterial in bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
John D. Krutty, Kevin Koesser, Stephen Schwartz, Junsu Yun, William L. Murphy, Padma Gopalan
Summary: This study created chemically defined three-dimensional surfaces for large-scale cell expansion by applying a synthetic polymer coating on commercially available polystyrene microcarriers, providing a reproducible surface without relying on xenogeneic serum proteins. These microcarriers not only improved the adhesion of hMSC in serum-free media but also allowed efficient cell passage without the use of cleavage enzymes.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biology
Samuel Herberg, Daniel Varghai, Daniel S. Alt, Phuong N. Dang, Honghyun Park, Yuxuan Cheng, Jung-Youn Shin, Anna D. Dikina, Joel D. Boerckel, Marsha W. Rolle, Eben Alsberg
Summary: This study demonstrates the potential of localized morphogen presentation to stimulate chondrogenic priming/endochondral differentiation in engineered hMSC tubes compared to loosely packed hMSC sheets for defect healing. hMSC tubes, unlike hMSC sheets, induced regenerate tissue partially resembling normal growth plate in long bone regeneration.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Kannan Manian, Chad A. Galloway, Sonal Dalvi, Anthony A. Emanuel, Jared A. Mereness, Whitney Black, Lauren Winschel, Celia Soto, Yiming Li, Yuanhui Song, William DeMaria, Akhilesh Kumar, Igor Slukvin, Michael P. Schwartz, William L. Murphy, Bela Anand-Apte, Mina Chung, Danielle S. W. Benoit, Ruchira Singh
Summary: Researchers have developed a 3D RPE-CC model that mimics key features of healthy and AMD/MD eyes while providing modular control over RPE and CC layers. They have found that both RPE and mesenchyme-secreted factors are necessary for the formation of fenestrated CC-like vasculature, and that CC atrophy and/or CNV can be initiated by patient serum or mutant RPE-secreted factors. Additionally, FGF2 and matrix metalloproteinases have been identified as potential therapeutic targets for AMD/MDs.
Article
Chemistry, Multidisciplinary
Aixiang Ding, Oju Jeon, David Cleveland, Kaelyn L. Gasvoda, Derrick Wells, Sang Jin Lee, Eben Alsberg
Summary: 4D bioprinting is a promising technique for creating complex cell-laden constructs for tissue/organ regeneration. This study introduces a new single-component micro-flake hydrogel (MFH) system as a bioink for 4D bioprinting. The MFH exhibits scalable production, simple composition, and shear-thinning, shear-yielding, and rapid self-healing properties.
ADVANCED MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
M. Orth, T. Fritz, J. Stutz, C. Scheuer, B. Ganse, Y. Bullinger, J. S. Lee, W. L. Murphy, M. W. Laschke, M. D. Menger, T. Pohlemann
Summary: MCM + VB, loaded with VEGF and BMP-2, improves bone healing in atrophic non-unions by promoting angiogenesis and osteogenesis, as well as reducing early osteoclast activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Aixiang Ding, Sang Jin Lee, Rui Tang, Kaelyn L. Gasvoda, Felicia He, Eben Alsberg
Summary: This study demonstrates a simple 4D bioprinting approach for the formation of shape-morphing cell condensate-laden bilayer systems with predefined complex shapes, broadening the applications of cell condensation-based 4D bioprinting.
Article
Engineering, Biomedical
Andrew S. Khalil, Daniel Hellenbrand, Kaitlyn Reichl, Jennifer Umhoefer, Mallory Filipp, Joshua Choe, Amgad Hanna, William L. Murphy
Summary: Spinal cord injuries often have devastating consequences and limited therapeutic options. This study utilizes mineral-coated microparticles as efficient non-viral gene delivery vehicles to produce exogenous ChABC in spinal cord injuries, reducing CSPG deposition and improving motor function recovery.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Cell & Tissue Engineering
Anna Laura Nelson, GianLuca Fontana, Elizabeth Miclau, Mallory Rongstad, William Murphy, Johnny Huard, Nicole Ehrhart, Chelsea Bahney
Summary: This article reviews methods to enhance bone formation and improve bone repair, focusing on the challenges and solutions for activating the Wnt pathway through antibody-based therapeutics, synthetic Wnt surrogates, and scaffold doping. Additionally, emerging technologies such as mRNA therapy and CRISPR technology for clinical translation are discussed.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Biophysics
Rui Tang, Kaelyn L. Gasvoda, Jacob Rabin, Eben Alsberg
Summary: Thermally stable proteins are important for resisting irreversible denaturation during thermal stress. In this study, the conformational and thermal stability of TGF-beta 1 were investigated, revealing its ability to recover native folding after thermal treatment. Further studies demonstrated the maintenance of its bioactive properties and potential applications in biomedicine and nutrition.
BIOPHYSICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Md Shahadat Hossan, Ethan Samuel Lin, Eleanor Riedl, Austin Stram, Eric Mehlhaff, Luke Koeppel, Jamie Warner, Inem Uko, Lori Mankowski Gettle, Sam Lubner, Stephanie M. McGregor, Wei Zhang, William Murphy, Jeremy D. Kratz
Summary: To accurately predict therapeutic responses in pancreatic and ampullary cancers, researchers have developed a low-volume screening assay and an automated alignment algorithm. By comparing the oligoclonal growth response with validated markers of response, subclonal populations and their relationship with treatment sensitivity were identified. This study is of great significance for utilizing cancer organoid models as biomarkers and advancing novel therapeutic development across different cancer types.
BIOENGINEERING-BASEL
(2023)
Article
Ophthalmology
Hannah Yoo, Ayushi Singh, Haiyan Li, Ana N. N. Strat, Tyler Bague, Preethi S. S. Ganapathy, Samuel Herberg
Summary: This study aimed to investigate the effect of simvastatin on glucocorticoid-induced human TM cell dysfunction by inhibiting YAP/TAZ activation. The results showed that simvastatin can effectively block the nuclear localization/activity of YAP/TAZ and attenuate the pathological phenotype of HTM cells, indicating that simvastatin may reduce the risk of developing glaucoma by indirectly inhibiting YAP/TAZ.
CURRENT EYE RESEARCH
(2023)
Article
Engineering, Biomedical
Junsu Yun, Samantha Robertson, Chanul Kim, Masatoshi Suzuki, William L. Murphy, Padma Gopalan
Summary: Decellularized plant scaffolds with innate uniaxial micropatterned topography were biofunctionalized with a copolymer to enable cell adhesion, growth, and alignment. Human muscle cells cultured on this biofunctionalized leaf scaffold showed aligned cell orientation and formation of muscle-like myotube structures, demonstrating potential for tissue engineering applications.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Sung-Won Hwang, Chung-Man Lim, Cong Truc Huynh, Hossein Moghimianavval, Nicholas A. Kotov, Eben Alsberg, Allen P. Liu
Summary: This study presents a strategy to confer mechano-responsive behavior on hydrogels by embedding hybrid vesicles. Under mechanical stress, these vesicles release encapsulated compounds that control the hydrogel network, offering promise for diverse biomedical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Kari Martyniak, Sean Kennedy, Makan Karimzadeh, Maria A. Cruz, Oju Jeon, Eben Alsberg, Thomas J. Kean
Summary: In this study, a high-throughput human articular chondrocyte reporter system was used to optimize the combination of GelMA and OMA for lubricin expression. The 14% GelMA / 2% OMA formulation was identified as the optimal one for lubricin secretion. This strategy allows for rapid analysis of the effects of biomaterial composition and cell manipulation on lubricin expression, which could enhance therapeutic strategies for cartilage regeneration.
BIOENGINEERING-BASEL
(2023)
Article
Materials Science, Biomaterials
Jae Sung Lee, Ping Guo, Katarina Klett, MacGregor Hall, Krishna Sinha, Sudheer Ravuri, Johnny Huard, William L. Murphy
Summary: Autologous platelet-rich plasma (PRP) is a popular treatment for musculoskeletal tissue injuries due to its favorable safety profile and cost-effectiveness. However, the therapeutic efficacy of PRP for osteoarthritis and damaged cartilage has been inconsistent. In this study, the researchers developed a method to enhance the efficacy of PRP treatment by attenuating a growth factor known to inhibit cartilage repair. The results showed that VEGF-attenuated PRP significantly improved healing of rat OA cartilage compared to conventional PRP treatment or control group. This study provides a new PRP customization method that can be applied to various tissue repair processes.
BIOMATERIALS SCIENCE
(2022)
