Article
Immunology
Jessica Schuster, Shi-Bin Cheng, James Padbury, Surendra Sharma
Summary: Pre-eclampsia is a hypertensive disease of pregnancy with mechanisms centered around abnormal placentation, inadequate spiral-artery remodeling, and deficiency in trophoblast invasion. Extracellular vesicles (EVs) are suggested to play a crucial role in pre-eclampsia, potentially influencing immune responses, inflammation, altered angiogenesis, and endothelial dysfunction.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Immunology
Chiara Tersigni, Donatella Lucchetti, Rita Franco, Filomena Colella, Caterina Neri, Laura Crispino, Alessandro Sgambato, Antonio Lanzone, Giovanni Scambia, Manu Vatish, Nicoletta Di Simone
Summary: PE is a common pregnancy disorder characterized by hypertension and proteinuria, with aberrant expression of HLA-DR in STBEVs specifically associated with PE. Further studies are needed to define the role of abnormal placental HLA-DR expression in PE pathogenesis and evaluate the potential application of detecting circulating HLA-DR positive STBEVs in diagnosing and predicting PE in early and mid-pregnancy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Miguel A. Ortega, Oscar Fraile-Martinez, Cielo Garcia-Montero, Alberto Paradela, Maria Asuncion Sanchez-Gil, Sonia Rodriguez-Martin, Juan A. De Leon-Luis, Claude Pereda-Cerquella, Julia Bujan, Luis G. Guijarro, Melchor Alvarez-Mon, Natalio Garcia-Honduvilla
Summary: The human placenta plays multiple roles in pregnancy and has a significant impact on both maternal and fetal well-being. Extracellular vesicles (EVs) are crucial for the development and functioning of the placenta, as well as for communication between the mother and fetus. These EVs have been identified as potential biomarkers for various obstetric pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Arshiya Parveen, Suman Mishra, Medha Srivastava, Dharmendra K. Chaudhary, Deepa Kapoor, Amrit Gupta, Swasti Tiwari
Summary: The study found that the transcriptome of placental alkaline phosphate positive exosomes changes with gestational age advancement in healthy women, expressing crucial placental genes involved in early embryonic development. These genes primarily participate in actin cytoskeleton organization, appropriate cell positioning, DNA replication, and regulation for protecting mammalian fetuses from rejection.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Joshua D. Spitzberg, Scott Ferguson, Katherine S. Yang, Hannah M. Peterson, Jonathan C. T. Carlson, Ralph Weissleder
Summary: This study developed a technique called MASEV for multiplexed analysis of EVs, which allows the interrogation of thousands of individual EVs using multi-channel fluorescence staining. The findings reveal the presence of multiple biomarkers in single vesicles and the potential loss of rare EV subtypes through affinity purification. These findings demonstrate the importance of MASEV in uncovering the heterogeneity and biology of EVs and improving diagnostic specificity.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Wenjing Liu, Yuanyu Huang, Zhengping Li, Lele Li, Yuliang Zhao, Mengyuan Li
Summary: This study presents an exosomal engineering strategy that enables controlled regulation of exosome-cell interactions and dynamic monitoring through activatable sensing probes. The engineered exosomes exhibit enhanced cancer cell targeting ability compared to conventional modifications. This approach could contribute to the development of exosome-based biomedical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sheng Yuan Leong, Hong Boon Ong, Hui Min Tay, Fang Kong, Megha Upadya, Lingyan Gong, Ming Dao, Rinkoo Dalan, Han Wei Hou
Summary: Extracellular vesicles are considered as next generation diagnostic biomarkers due to their disease-specific biomolecular cargoes and importance in cell-cell communications. This study presents a microfluidic platform for EV-protein fractionation using size exclusion chromatography. The device shows excellent performance in isolating EVs from biological samples and has been validated for clinical applications.
Article
Biochemical Research Methods
Sandrine Reymond, Lyssia Gruaz, Jean-Charles Sanchez
Summary: This study aimed to improve the efficiency and protein coverage of plasma-derived extracellular vesicles (EVs) by removing highly abundant plasma proteins. Depleted EVs showed higher particle concentrations and a broader protein coverage. However, EVs from non-depleted plasma carried slightly more EV markers. This study also demonstrated the clinical feasibility of characterizing the proteome of EVs derived from small plasma volumes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maria Solaguren-Beascoa, Ana Gamez-Valero, Georgia Escaramis, Marina Herrero-Lorenzo, Ana M. Ortiz, Carla Minguet, Ricardo Gonzalo, Maria Isabel Bravo, Montserrat Costa, Eulalia Marti
Summary: Small RNAs (sRNAs) are bioactive molecules that can be detected in biofluids, reflecting physiological and pathological states. Phospho-RNA-seq, a recent method, increases the detection of previously inaccessible RNAs. This study found that phospho-RNA-seq is valuable in uncovering RNA-based biomarkers in extracellular vesicles (EVs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Y. Chen, P. Huang, C. Han, J. Li, L. Liu, Z. Zhao, Y. Gao, Y. Qin, Q. Xu, Y. Yan, Y. Wang, J. Ren, J. Men, J. Dong, J. Zhang, F. Xue
Summary: This study found that placental, platelet-, and endothelial cell-derived extracellular vesicles (EVs) were significantly elevated in pre-eclampsia (PE) patients, but only placental-derived EVs could predict PE. These EVs played a causal role in the pregnancy-induced hypercoagulability.
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2021)
Article
Medicine, Research & Experimental
Hyun-Kyung Woo, Young Kwan Cho, Chang Yeol Lee, Haeun Lee, Cesar M. Castro, Hakho Lee
Summary: Extracellular vesicles (EVs) have the potential for disease diagnosis, but are often contaminated by low density lipoproteins (LDLs) when isolated from blood. By studying the difference in electric charge between EVs and LDLs, we developed a method using ion-exchange chromatography to separate EVs from LDLs. This finding improved the analytical signals in EV molecular assays.
Article
Oncology
Eleni-Kyriaki Vetsika, Priyanka Sharma, Ioannis Samaras, Alexandra Markou, Vassilis Georgoulias, Theresa L. Whiteside, Athanasios Kotsakis
Summary: This study found that pre-therapy total sEV protein levels in plasma are associated with clinical outcomes in treatment-naive NSCLC patients, with high levels serving as significant negative predictors of PFS and OS. Additionally, there is a positive correlation between sEV TEP levels and the percentages of circulating CD8(+)PD-1(+) and CD8(+)PD-L1(+)T cells, suggesting sEV may hinder anti-tumor immune responses through the PD-L1/PD-1 pathway. Pre-therapy plasma sEV levels could potentially be used as non-invasive predictors of NSCLC progression and treatment outcomes.
Article
Cell Biology
Wenyi Zheng, Rui He, Xiuming Liang, Samantha Roudi, Jeremy Bost, Coly Pierre-Michael, Guillaume van Niel, Samir E. L. Andaloussi
Summary: This study presents a reconfigurable glycoengineering strategy to produce extracellular vesicles (EVs) with strong cellular specificity by endogenously displaying glycans of interest on the EV surface. The strategy showcases the surface display of different glycan ligands on EVs and achieves high specificity towards specific cell types, leading to enhanced therapeutic effects and attenuation of cell damage.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Medicine, General & Internal
Sanja Lalic-Cosic, Violeta Dopsaj, Mirjana Kovac, Vesna Mandic-Markovic, Zeljko Mikovic, Fariborz Mobarrez, Aleksandra Antovic
Summary: The study found that in pregnant women with pre-eclampsia (P-EC), the concentration of extracellular vesicles (EVs) expressing specific markers was elevated and associated with global hemostatic parameters and fibrin clot properties. This suggests that EVs may be involved in intravascular fibrin deposition leading to subsequent microcirculation disorders.
FRONTIERS IN MEDICINE
(2021)
Article
Immunology
Deborah Neyrinck-Leglantier, Marie Tamagne, Sasha L'honore, Leonie Cagnet, Sadaf Pakdaman, Alexandre Marchand, France Pirenne, Benoit Vingert
Summary: Autologous EVs may modulate the function of immune system cells, specifically CD4(+) T lymphocytes, but their immunomodulatory role is different from heterologous EVs. Autologous EVs can increase CD4(+) T lymphocyte proliferation, but T-cell receptor engagement is required.
FRONTIERS IN IMMUNOLOGY
(2022)