期刊
TRANSGENIC RESEARCH
卷 23, 期 1, 页码 177-185出版社
SPRINGER
DOI: 10.1007/s11248-013-9764-x
关键词
Mouse; Genetics; Transgenics; Mammalian; Cre recombinase
类别
资金
- Wellcome Trust [WT098051]
- NIH [U42RR033192]
We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据