Article
Biochemistry & Molecular Biology
Yunfeng Yan, Xiaofei Huang, Yao Zhou, Jingyi Li, Feiyun Liu, Xueying Li, Xiaotao Hu, Jing Wang, Lingyan Guo, Renning Liu, Naoki Takaya, Shengmin Zhou
Summary: In Aspergillus nidulans, the peroxiredoxin (PrxA) plays a crucial role in protecting dormant conidia against high levels of H2O2, while catalase (CatB) becomes the main antioxidant during the mature hyphae stage. CatB needs to be secreted to function, and this process is limited to the mature hyphae stage.
Article
Food Science & Technology
Ming-Hsun Shen, Rakesh K. Singh
Summary: This study investigated the effect of H2O2 treatment on catalase-inactivated peanuts and found that it effectively reduced aflatoxins. Higher treatment temperature and catalase inactivation improved the efficacy of AF reduction. The oil quality of treated peanuts was not significantly affected. The weight loss and oil quality change of the treated peanuts were negligible. Peanuts were able to keep intact after the treatment.
Article
Chemistry, Analytical
Lu Li, Jingjing Xu, Jingru Li, Xin Lyu, Zhanfang Liu, Juan Wei
Summary: Polymeric photonic crystals containing ferrocene were successfully prepared and used for efficient visual detection of hydrogen peroxide and catalase. The variations in the morphology of ferrocene before and after redox resulted in volume shrinkage of the sensors and a significant blue shift in reflection. The sensors exhibited excellent performance in detecting hydrogen peroxide and catalase.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Abeera Shaeer, Mehwish Aslam, Naeem Rashid
Summary: In this study, a putative manganese-catalase gene Cat(Bsu) from Bacillus subtilis R5 was cloned and expressed in Escherichia coli, and its biochemical characteristics were described. Cat(Bsu) exhibited highest activity at pH 8.0 and 55 degrees C, with a half-life of 2 hours at 80 degrees C.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Kanokwan Srirattana, Masahiro Kaneda, Rangsun Parnpai
Summary: This article reviews recent approaches for improving the technical aspects of somatic cell nuclear transfer (SCNT) and enhancing epigenetic modifications in donor cells, oocytes, and cloned embryos in order to increase cloning efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fang Yuan, Shouliang Yin, Yang Xu, Lijun Xiang, Haiyan Wang, Zilong Li, Keqiang Fan, Guohui Pan
Summary: Catalases in bacteria have a crucial role in defense against oxidative stress and are involved in various cellular processes. The study revealed the diversity and distribution of catalases among bacterial genomes, highlighting their importance in secondary metabolite biosynthesis. Further investigation into the physiological roles of catalases may aid in enhancing the yield of valuable secondary metabolites in engineered bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Shaoqing Lu, Yongxian Guo, Lubin Qi, Qiongzheng Hu, Li Yu
Summary: A H2O2-responsive liquid crystal sensing platform was developed and utilized for detecting catalase in human serum, showing high sensitivity and label-free detection capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Manabu Nakazono, Kenji Hamase
Summary: Four novel bis-acridinium esters were synthesized and their chemiluminescence properties were studied. The introduction of fluorine and methyl groups at specific positions greatly affected the chemiluminescence intensities. One of the esters showed strong and long-lasting chemiluminescence under neutral and alkaline conditions and was used for sensitive determination of hydrogen peroxide and catalase activity. The linear calibration ranges for hydrogen peroxide and catalase activity were 5-1000 μM and 0.05-1 U/mL, respectively.
Article
Reproductive Biology
Young-Bum Son, Yeon Ik Jeong, Kyu Chan Hwang, Yeon Woo Jeong, Woo Suk
Summary: This study evaluated the developmental potential of interspecies lycaon-dog embryos and assessed the mitochondrial content and metabolism of the produced cloned lycaon-dog fetus. Despite the genetic identity between the cloned lycaon-dog fetus and lycaon donor cell, mitochondrial DNA was inherited from the oocyte donor dogs. The study suggested that nuclear-mitochondrial incompatibility may contribute to fetal death due to impaired pyruvate metabolism.
Article
Biotechnology & Applied Microbiology
Javier Espinoza-Vergara, Paulo Molina, Mariana Walter, Miguel Gulppi, Nelson Vejar, Francisco Melo, Marcela Urzua, Hugo Munoz, Jose H. Zagal, Xiaorong Zhou, Manuel I. Azocar, Maritza A. Paez
Summary: The study investigated the influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa. It was found that the enzymatic activity exhibited maximum activity at pH 7.5, with oxygen reduction reaction (ORR) occurring at higher overpotentials at higher or lower pHs. Atomic force microscopy was also used to assess the influence of bacterial adhesion on the electrochemical behavior.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ouardia Bendou, Ismael Gutierrez-Fernandez, Emilio L. Marcos-Barbero, Nara Bueno-Ramos, Ana I. Gonzalez-Hernandez, Rosa Morcuende, Juan B. Arellano
Summary: A rapid and high throughput protocol has been developed to measure the catalase activity in vitro. By using a two-degree polynomial fitting of the experimental data, the initial rate of H2O2 decomposition by catalase can be determined, and statistical bias can be avoided. This protocol can be used for small scale and low cost measurement of other antioxidant enzyme activities in microplate readers.
Article
Reproductive Biology
Van Khanh Nguyen, Tamas Somfai, Daniel Salamone, Vu Thi Thu Huong, Huong Le Thi Nguyen, Quan Xuan Huu, Au Thi Hoang, Hieu Trung Phan, Yen Kim Thi Pham, Lan Doan Pham
Summary: An efficient culture system was established to produce embryos by SCNT of the endangered Vietnamese ? pig, achieving a blastocyst production rate of 26.4% by improving culture systems for donor cells, oocytes, and embryos culture. Transfer of embryos resulted in pregnancies, but live offspring were not obtained.
Article
Chemistry, Analytical
Mauro Tomassetti, Riccardo Pezzilli, Giuseppe Prestopino, Corrado Di Natale, Pier Gianni Medaglia
Summary: An enzyme biosensor and a non-enzymatic sensor were fabricated for detecting and catalyzing H2O2 using LDH glued on GC electrodes. Both sensors exhibited long lifetimes, but the enzyme biosensor showed a lower detection limit compared to the non-enzymatic sensor.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Seyyed Jaber Hosseini, Zeinolabedin Tahmasebi-Sarvestani, Ali Mokhtassi-Bidgoli, Hamed Keshavarz, Shahryar Kazemi, Masoumeh Khalvandi, Hematollah Pirdashti, Seyyed Hamidreza Hashemi-Petroudi, Silvana Nicola
Summary: Saline stress significantly affects plant growth, especially in drought-affected areas. This study examined the biochemical and enzymatic properties of 18 mint ecotypes, and found that M. piperita and M. rotundifolia exhibited better stress tolerance, while M. longifolia was salt sensitive. The results provide valuable information for selecting and breeding salt-tolerant mint varieties.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Chemistry, Analytical
Shaoqing Lu, Qiongzheng Hu, Li Yu
Summary: A brief, convenient, label-free and sensitive liquid crystal sensing platform is demonstrated for the detection of catalase in human serum. CeO2 nanomaterials and single-stranded DNA (ssDNA) are used to detect catalase by changing the optical appearance of liquid crystals.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Paula R. Chen, Lee D. Spate, Eric C. Leffeler, Joshua A. Benne, Raissa F. Cecil, Taylor K. Hord, Randall S. Prather
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Biochemistry & Molecular Biology
Raissa F. Cecil, Paula R. Chen, Joshua A. Benne, Taylor K. Hord, Lee D. Spate, Melissa S. Samuel, Randall S. Prather
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Biochemical Research Methods
C. G. Lucas, A. M. Spate, M. S. Samuel, L. D. Spate, W. C. Warren, R. S. Prather, K. D. Wells
TRANSGENIC RESEARCH
(2020)
Article
Medicine, Research & Experimental
Eric M. Walters, Christopher Burlak
XENOTRANSPLANTATION
(2020)
Article
Medicine, Research & Experimental
Erik A. Koppes, Bethany K. Redel, Marie A. Johnson, Kristen J. Skvorak, Lina Ghaloul-Gonzalez, Megan E. Yates, Dale W. Lewis, Susanne M. Gollin, Yijen L. Wu, Shawn E. Christ, Martine Yerle, Angela Leshinski, Lee D. Spate, Joshua A. Benne, Stephanie L. Murphy, Melissa S. Samuel, Eric M. Walters, Sarah A. Hansen, Kevin D. Wells, Uta Lichter-Konecki, Robert A. Wagner, Joseph T. Newsome, Steven F. Dobrowolski, Jerry Vockley, Randall S. Prather, Robert D. Nicholls
Article
Multidisciplinary Sciences
Katy S. Stoecklein, M. Sofia Ortega, Lee D. Spate, Clifton N. Murphy, Randall S. Prather
Summary: This study investigated the potential of adding a combination of three cytokines (FGF2, LIF, and IGF1) named FLI to improve the development and quality of in vitro produced bovine embryos. Results showed that the addition of FLI increased the success rate of oocyte maturation and blastocyst development, as well as improved the quality of embryos by reducing lipid content and enhancing cytoskeleton integrity. This supplementation also decreased cell apoptosis post-thaw, indicating its potential to alleviate challenges associated with cryo-survival of in vitro produced bovine embryos.
Article
Multidisciplinary Sciences
Krishanu Saha, Erik J. Sontheimer, P. J. Brooks, Melinda R. Dwinell, Charles A. Gersbach, David R. Liu, Stephen A. Murray, Shengdar Q. Tsai, Ross C. Wilson, Daniel G. Anderson, Aravind Asokan, Jillian F. Banfield, Krystof S. Bankiewicz, Gang Bao, Jeff W. M. Bulte, Nenad Bursac, Jarryd M. Campbell, Daniel F. Carlson, Elliot L. Chaikof, Zheng-Yi Chen, R. Holland Cheng, Karl J. Clark, David T. Curiel, James E. Dahlman, Benjamin E. Deverman, Mary E. Dickinson, Jennifer A. Doudna, Stephen C. Ekker, Marina E. Emborg, Guoping Feng, Benjamin S. Freedman, David M. Gamm, Guangping Gao, Ionita C. Ghiran, Peter M. Glazer, Shaoqin Gong, Jason D. Heaney, Jon D. Hennebold, John T. Hinson, Anastasia Khvorova, Samira Kiani, William R. Lagor, Kit S. Lam, Kam W. Leong, Jon E. Levine, Jennifer A. Lewis, Cathleen M. Lutz, Danith H. Ly, Samantha Maragh, Paul B. McCray, Todd C. McDevitt, Oleg Mirochnitchenko, Ryuji Morizane, Niren Murthy, Randall S. Prather, John A. Ronald, Subhojit Roy, Sushmita Roy, Venkata Sabbisetti, W. Mark Saltzman, Philip J. Santangelo, David J. Segal, Mary Shimoyama, Melissa C. Skala, Alice F. Tarantal, John C. Tilton, George A. Truskey, Moriel Vandsburger, Jonathan K. Watts, Kevin D. Wells, Scot A. Wolfe, Qiaobing Xu, Wen Xue, Guohua Yi, Jiangbing Zhou
Summary: The NIH's SCGE Consortium aims to develop safer and more effective methods to edit disease-relevant somatic cell genomes in patients, even in hard-to-reach tissues. Their approach includes rigorous validation of technology through third-party testing in animals to accelerate clinical development of new therapies.
Review
Developmental Biology
Rodney D. Geisert, Ashley E. Meyer, Caroline A. Pfeiffer, Destiny N. Johns, Kiho Lee, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: The development of viviparity in mammals requires the evolution of the placenta as an intermediate interface between the fetus and maternal uterus, as well as the modification or inhibition of the maternal immune system. CRISPR-Cas9 gene-editing technology has provided a direct approach to investigate the specific role of conceptus factors in pregnancy establishment and maintenance, altering our understanding of maternal recognition and maintenance of pregnancy in pigs.
Article
Biochemistry & Molecular Biology
Paula R. Chen, Caroline G. Lucas, Lee D. Spate, Randall S. Prather
Summary: Supplementation with glutamine in porcine embryo culture medium improves development, increases leucine consumption, and enhances mitochondrial activity. Glutaminolysis is involved in the activation of mTORC1 in porcine embryos, as evidenced by changes in phosphorylated MTOR levels and colocalization with lysosomal markers. Further studies are needed to understand the downstream effects on development.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Article
Cardiac & Cardiovascular Systems
George C. Gabriel, William Devine, Bethany K. Redel, Kristin M. Whitworth, Melissa Samuel, Lee D. Spate, Raissa F. Cecil, Randall S. Prather, Yijen Wu, Kevin D. Wells, Cecilia W. Lo
Summary: Modeling cardiovascular diseases in pigs has revealed similar cardiac development processes to humans and mice, showing potential for preclinical studies focusing on congenital heart disease improvement. Genetically edited pigs with congenital heart defects could serve as valuable models for further research.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Reproductive Biology
C. G. Lucas, B. K. Redel, P. R. Chen, L. D. Spate, R. S. Prather, K. D. Wells
Summary: Pigs have become important models for agricultural and biomedical purposes, with the use of genomic engineering tools like CRISPR/Cas9 to produce livestock models with desired modifications. Enhancing HDR events in pig embryos using the compound RS-1 has shown promising results, with no adverse effects on early embryo development. Combining CRISPR/Cas9 with HDR strategies, like utilizing RS-1, is a key tool to simplify and accelerate the production of genetically modified livestock models.
REPRODUCTIVE TOXICOLOGY
(2021)
Review
Cell Biology
Paula R. Chen, Bethany K. Redel, Karl C. Kerns, Lee D. Spate, Randall S. Prather
Summary: The review discusses the application of genetically modified pigs in animal agriculture and human medicine, focusing on the importance of in vitro production and manipulation of embryos. To improve porcine embryo culture systems, consideration of metabolic characteristics, comparisons with other mammalian species, and addressing stressors from environment and parental factors are essential. Progressing stepwise through in vitro oocyte maturation, fertilization, and embryo culture in pigs can help assess current systems and identify areas for improvement.
Review
Biochemistry & Molecular Biology
Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: CRISPR-Cas9 gene editing technology allows for the study of specific genes involved in reproductive regulation, particularly in the establishment and maintenance of pregnancy. Research on gene editing of porcine conceptuses provides new insights into the regulation of endometrial function, while in vitro studies using endometrial organoids offer an alternative method for studying specific endometrial genes.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Cardiac & Cardiovascular Systems
Katherine Bishara, Jennie H. H. Kwon, Morgan A. A. Hill, Kristi L. L. Helke, Russell A. A. Norris, Kristin Whitworth, Randall S. S. Prather, Taufiek Konrad Rajab
Summary: A transgenic strain of pigs expressing green fluorescent protein (GFP) was created, and GFP expression in the semilunar valves and great arteries was characterized. Immunofluorescence was used to visualize and quantify GFP expression and its colocalization with nuclear staining. GFP expression was confirmed in both the semilunar valves and great arteries of the transgenic pigs.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Review
Agriculture, Multidisciplinary
Kristin M. M. Whitworth, Jonathan A. A. Green, Bethany K. K. Redel, Rodney D. D. Geisert, Kiho Lee, Bhanu P. P. Telugu, Kevin D. D. Wells, Randall S. S. Prather
Summary: Genetic modification of animals through selective breeding is essential for modern agriculture, but it has limitations. In order to sustainably improve animal production and feed the growing global population, responsive and precise breeding strategies are needed. The emergence of genome editing tools provides a solution, and germline engineering in pigs is a significant development in this field.
CABI AGRICULTURE & BIOSCIENCE
(2022)