期刊
TRANSGENIC RESEARCH
卷 18, 期 3, 页码 455-465出版社
SPRINGER
DOI: 10.1007/s11248-008-9236-x
关键词
Auto-excision; FLP/FRT recombination system; Marker-free; Oxidative stress-inducible; Transgenic tobacco
类别
资金
- National Institute of Agricultural Biotechnology [05-5-11-22-2]
- BioGreen21 program [20070501-034-005]
- Rural Development Administration, Korea
Antibiotic resistance marker genes are powerful selection tools for use in plant transformation processes. However, once transformation is accomplished, the presence of these resistance genes is no longer necessary and can even be undesirable. We herein describe the successful excision of antibiotic resistance genes from transgenic plants via the use of an oxidative stress-inducible FLP gene. FLP encodes a recombinase that can eliminate FLP and hpt selection genes flanked by two FRT sites. During a transformation procedure in tobacco, transformants were obtained by selection on hygromycin media. Regenerants of the initial transformants were screened for selective marker excision in hydrogen peroxide supplemented media and both the FLP and hpt genes were found to have been eliminated. About 13-41% of regenerated shoots on hydrogen peroxide media were marker-free. This auto-excision system, mediated by the oxidative stress-inducible FLP/FRT system to eliminate a selectable marker gene can be very readily adopted and used to efficiently generate marker-free transgenic plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据