Article
Biochemistry & Molecular Biology
Anshika Narang, Salvatore A. E. Marras, Natalia Kurepina, Varsha Chauhan, Elena Shashkina, Barry Kreiswirth, Mandira Varma-Basil, Christopher Vinnard, Selvakumar Subbian
Summary: The emergence of drug-resistant tuberculosis is a global health issue. This study developed a SuperSelective primer-based real-time PCR assay to efficiently diagnose heteroresistant tuberculosis, which can contribute to managing antimicrobial resistance in tuberculosis and other infectious diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Che Jie, Chen Bo Han, Xu Li, Gao Yuan, Yue Meng Meng, Chen Zi Man, Zhang Mao Jun, Shao Zhu Jun
Summary: This study established and modified 27 novel qPCR assays for the analysis of 97 pneumococcal serotypes. By performing bioinformatics analyses and experimental validation, specific gene sequences related to different serotypes were identified, allowing for the identification of multiple serotypes.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2023)
Article
Immunology
Gwynngelle A. Borillo, Ron M. Kagan, Elizabeth M. Marlowe
Summary: This study demonstrates that multiplexed real-time RT-PCR genotyping has strong agreement with whole-genome sequencing (WGS) and may provide additional SARS-CoV-2 variant screening capabilities when WGS is unavailable or cost-prohibitive. RT-PCR genotyping assays can also supplement existing sequencing efforts, providing rapid results at or near the time of diagnosis to guide patient management.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
K. Srividhyaa, S. Ranjani, S. Hemalatha
Summary: Antibiotic resistance is a major threat to global health, food security, and the environment. This study evaluated the protein binding capability of lemon peel phytochemicals against various poultry pathogens using molecular docking, and tested the antibacterial activity of LP AgNPs obtained from lemon peel. The results showed that LP AgNPs have the potential to treat antibiotic resistant poultry pathogens.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Theodoros Chatzimitakos, Vassilis Athanasiadis, Konstantina Kotsou, Eleni Bozinou, Stavros I. Lalas
Summary: This study aimed to identify the optimal extraction procedures and parameters to obtain the maximum quantity of bioactive components from lemon peel by-products. The results revealed that simple stirring for 150 min at 20 degrees C proved to be the most effective and practical method. Under optimum extraction conditions, the extract was found to contain high total phenolic content, total flavonoid content, amounts of ascorbic acid, and total carotenoids content, and demonstrated potent antioxidant properties, making it a promising ingredient for functional foods and cosmetics.
Article
Biochemistry & Molecular Biology
Valeria Sorrenti, Valeria Consoli, Salvo Grosso, Marco Raffaele, Margherita Amenta, Gabriele Ballistreri, Simona Fabroni, Paolo Rapisarda, Luca Vanella
Summary: The lemon extract showed potential in increasing PPARγ, GLUT4, and DGAT-1 levels, reducing IL-6 release, and restoring triglyceride synthesis, which could help manage insulin resistance conditions associated with TNF-alpha pathway activation.
Article
Ecology
Marco Di Domenico, Valentina Curini, Riccardo Caprioli, Carla Giansante, Agata Mrugala, Michaela Mojzisova, Cesare Camma, Adam Petrusek
Summary: The study developed real-time PCR TaqMan assays to distinguish five genotype groups of A. astaci. This method may be a useful tool for investigating the natural history of crayfish plague in Europe, as well as the sources and introduction pathways of the pathogen.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Gamal Hamad, Elsayed E. Hafez, Sherien E. Sobhy, Taha Mehany, Reham A. Elfayoumy, Eman M. Elghazaly, Michael Eskander, Rasha G. Tawfik, Saleh M. Hussein, Leonel Pereira
Summary: This study aimed to detect and control Clostridium botulinum in Egyptian fish products using natural leaf extracts of Citrus limon, Citrus sinensis, and Citrus unshiu, as well as investigating the application of C. limon in tuna. The antibacterial activity of C. limon leaf extract was also assessed. Results showed variations in the distribution of positive C. botulinum in fish samples from different governorates. All three Citrus extracts exhibited antimicrobial properties, with C. limon being the most effective. The C. limon leaf extract demonstrated high antioxidant and anti-Clostridium powers, suggesting its potential as a natural preservative in fishery products.
Article
Multidisciplinary Sciences
Chika Kingsley Onwuamah, Azuka Patrick Okwuraiwe, Olumuyiwa B. Salu, Joseph O. Shaibu, Nnaemeka Ndodo, Samuel O. Amoo, Leona C. Okoli, Fehintola A. Ige, Rahaman A. Ahmed, Munir Akinwale Bankole, Judith O. Sokei, Bamidele Paul Mutiu, James Ayorinde, Babatunde Akeem Saka, Celestina Obiekea, Nwando Mba, Richard A. Adegbola, Sunday Omilabu, Chikwe Ihekweazu, Babatunde Lawal Salako, Rosemary Audu
Summary: Quality diagnostics is crucial in containing the spread of SARS-CoV-2 infection, and this study compares the performance of five real-time diagnostic assays. The assays were effective in detecting high positive samples, but varied in identifying negative samples. The results can guide laboratories in determining repeat testing thresholds and cut-off values.
Article
Fisheries
Juliano dos Santos Macedo, Carlos Eduardo Copatti, Emmanoel Vilaca Costa, Felipe Moura Araujo da Silva, Livia Macedo Dutra, Victoria Laysna dos Anjos Santos, Jackson Roberto Guedes da Silva Almeida, Marcos Tavares-Dias, Jose Fernando Bibiano Melo
Summary: This study aimed to investigate the effects of dietary supplementation with Citrus limon extract (CLE) on the growth and immune response of striped catfish juveniles. The results showed that adding 0.4 g CLE/kg feed improved the growth and immune resistance of striped catfish.
AQUACULTURE INTERNATIONAL
(2023)
Article
Horticulture
Qi Wu, Mingming Zhao, Yi Li, Dazhi Li, Xianfeng Ma, Ziniu Deng
Summary: In this study, the BAK1 gene was identified as a disease resistance gene involved in plant-microbe interaction between citrus and Xcc. Functional investigations of this gene revealed that both CsBAK1 or CmBAK1 could inhibit the growth of Xcc to some extent. Critical regions of the CmBAK1 promoter sequence were identified, and a transcription factor named CmRAP2-13 was found to activate the expression of CmBAK1. Transcriptional analysis showed an increase in CsRAP2-13 levels in Citron C-05, American citron, and Finger citron.
Article
Biochemical Research Methods
Yahui Wang, Qing Wang, Sven M. Bergmann, Yingying Li, Bo Li, Yuefeng Lv, Jiyuan Yin, Guang Yang, Yang Qv, Yingying Wang, Weiwei Zeng
Summary: Both real-time RPA and qPCR assays showed high sensitivity and specificity for TiLV, with qPCR being more suitable for quantitative analysis and accurate detection in a diagnostic laboratory, while real-time RPA is more suitable for clinical disease diagnosis and preliminary screening in poorly equipped laboratories or fish farms.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Infectious Diseases
Olga Byadovskaya, Yana Pestova, Aleksandr Kononov, Irina Shumilova, Svetlana Kononova, Alexander Nesterov, Shawn Babiuk, Alexander Sprygin
Summary: This study evaluated the effectiveness of different assays for identifying lumpy skin disease virus, finding that when using recombinant viruses, there may be misclassification leading to inaccurate identification of vaccine or wild-type viruses.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Editorial Material
Forestry
Gaetano Distefano
Article
Chemistry, Applied
Giulia Modica, Claudia Pannitteri, Mario Di Guardo, Stefano La Malfa, Alessandra Gentile, Giuseppe Ruberto, Luana Pulvirenti, Lucia Parafati, Alberto Continella, Laura Siracusa
Summary: The study evaluated the impact of rootstock on fruit quality of sweet orange, revealing significant effects of temperature on polyphenol accumulation and antioxidant activity, as well as differences in metabolic profiles of juice among different rootstocks. Additionally, specific rootstocks were found to enhance anthocyanin accumulation in the fruit, providing valuable insights for rootstock selection in new plantings.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Agronomy
Ivana Castello, Alessandro D'Emilio, Andrea Baglieri, Giancarlo Polizzi, Alessandro Vitale
Summary: Pre-plant soil fumigation is a widely adopted method in intensive ornamental and vegetable production areas to manage soilborne pathogens. This study evaluated the effectiveness of dazomet applied alone and under virtually impermeable film (VIF) in managing Verticillium wilt on chrysanthemum plants. The results showed that a reduced rate of dazomet combined with VIF application had good performances in controlling the disease and reducing yield losses, suggesting its potential use on a large scale.
Article
Biochemistry & Molecular Biology
Luigi Parrotta, Lavinia Mareri, Iris Aloisi, Claudia Faleri, Gaetano Distefano, Alessandra Gentile, Angela Roberta Lo Piero, Verena Kriechbaumer, Marco Caruso, Giampiero Cai, Stefano Del Duca
Summary: This study investigated the effects of clementine ASP-RICH proteins on pollen tubes by expressing them in tobacco. The results showed that ASP-RICH modulated Ca2+ content, which in turn affected cytoskeleton organization and cell wall deposition, ultimately impacting the growth pattern of pollen tubes. These findings suggest a regulatory role of ASP-RICH in the mechanism of plant cell growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Giorgio Gusella, Alessandro Vitale, Giancarlo Polizzi
Summary: This study evaluated the efficacy of different biological control agents against canker pathogens of pistachio. Results showed that all the tested biological agents were able to inhibit the mycelial growth of the pathogens in vitro, but had limited effect in reducing infections in dynamic experiments. Only a few specific pathogens showed partial inhibition. Detached fruit experiments demonstrated that these biological control agents had a certain efficacy in reducing lesions caused by the pathogen Neofusicoccum mediterraneum.
Article
Plant Sciences
Francesca Calderone, Alessandro Vitale, Salvina Panebianco, Monia Federica Lombardo, Gabriella Cirvilleri
Summary: Organic viticulture has gained significant interest in recent years, and this study evaluated the effectiveness of alternative and sustainable methods for combating grapevine diseases. The combination of COS-OGA and AMF showed promising results in reducing powdery mildew severity, as well as protecting against gray mold and sour rot. Furthermore, this combination did not negatively impact grape quality and microbial communities. Overall, COS-OGA and AMF could be a viable and safer option for the sustainable management of grapevine diseases in organic farming systems.
Article
Agronomy
Damiano Puglisi, Giuseppina Las Casas, Filippo Ferlito, Elisabetta Nicolosi, Mario Di Guardo, Francesco Scollo, Giuseppe Saitta, Stefano La Malfa, Alessandra Gentile, Gaetano Distefano
Summary: The development of new seedless cultivars is an important goal in grape breeding programs. This study compares the efficiency of embryo rescue and gamic propagation techniques for obtaining new seedless cultivars. The results suggest that some genotypes perform better as female parents in terms of embryo/seed production. The research aims to provide useful information for improving the efficiency of seedless grape breeding programs.
Review
Biochemistry & Molecular Biology
Luca Nerva, Lorenza Dalla Costa, Angelo Ciacciulli, Silvia Sabbadini, Vera Pavese, Luca Dondini, Elisa Vendramin, Emilia Caboni, Irene Perrone, Andrea Moglia, Sara Zenoni, Vania Michelotti, Sabrina Micali, Stefano La Malfa, Alessandra Gentile, Stefano Tartarini, Bruno Mezzetti, Roberto Botta, Ignazio Verde, Riccardo Velasco, Mickael Arnaud Malnoy, Concetta Licciardello
Summary: Climate change is affecting food chain production, leading to reduced quality and yield. The international scientific community is working on enhancing resilience in agriculture. Italy, a major European producer of fruit trees, has taken initiatives to maintain the specificity of the 'Made in Italy' label. However, conventional breeding approaches face challenges in terms of financial commitment, land resources availability, and long generation times.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Agronomy
Sebastiano Seminara, Stefania Bennici, Mario Di Guardo, Marco Caruso, Alessandra Gentile, Stefano La Malfa, Gaetano Distefano
Summary: This study reviews the current status of sweet orange varieties and the main driving forces in sweet orange breeding, and points out that genetic diversity within the species is derived from mutations and hybridization and backcrossing between mandarin and pummelo. It also introduces the application of conventional and innovative (molecular-based) methods in breeding improved sweet orange varieties.
Article
Agronomy
Chiara Catalano, Giuseppina Las Casas, Alessio Giuffrida, Filippo Ferlito, Mario Di Guardo, Alberto Continella, Stefania Bennici, Stefano La Malfa, Alessandra Gentile, Gaetano Distefano
Summary: The study characterized the high variability in floral sterility and polyembryony traits among forty Sicilian and international lemon cultivars, with 'Adamopoulos' showing the highest polyembryonic seed percentage. The results provide valuable information for breeders to design novel breeding schemes.
Editorial Material
Plant Sciences
Alessandro Vitale
Article
Horticulture
Claudio Di Vaio, Aurora Cirillo, Chiara Catalano, Stefano La Malfa
Summary: This study aims to explore the germplasm resources of lemons in Southern Italy and identify cultivars suitable for fresh consumption and processed goods. The study found significant diversity among different cultivars, with some cultivars being suitable for fresh consumption and others for processed goods.