Review
Cardiac & Cardiovascular Systems
Junqi Wang, Xiaoping Chen
Summary: This review summarizes the roles of JAMs in the development of atherosclerosis and predicts future research directions.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell Biology
Max L. B. Gronloh, Janine J. G. Arts, Jaap D. van Buul
Summary: Leukocyte transendothelial migration (TEM) during inflammation involves several established steps, such as rolling, adhesion, crawling, diapedesis, and sub-endothelial crawling. Factors like chemokine cues, adhesion molecules, membrane protrusions, and physical factors contribute to the formation of transmigration hotspots where leukocytes exit the vasculature.
JOURNAL OF CELL SCIENCE
(2021)
Review
Cardiac & Cardiovascular Systems
Carina Mauersberger, Julia Hinterdobler, Heribert Schunkert, Thorsten Kessler, Hendrik B. Sager
Summary: This article comprehensively reviews the process of leukocyte recruitment relevant to atherosclerosis, with a focus on endothelial cells, monocytes and macrophages, neutrophils, T lymphocytes and platelets. Therapeutic options targeting leukocyte recruitment into atherosclerotic lesions, as well as potential insights into its precise mechanisms, are also highlighted.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Sreelakshmi N. Menon, Farzana Zerin, Emmanuella Ezewudo, Nimi P. Simon, Sreeranjini N. Menon, Morgan L. Daniel, Andrea J. Green, Ajay Pandey, Charles E. Mackay, Sherif Hafez, Nader H. Moniri, Raquibul Hasan
Summary: Neflamapimod effectively inhibits endothelial cell activation, adhesion molecule expression, and leukocyte attachment, thereby reducing vascular inflammation.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Oncology
Marina Cerreto, Robin Foa, Alessandro Natoni
Summary: The progression of chronic lymphocytic leukemia (CLL) and its response to therapies depend on the microenvironment of the bone marrow and lymph nodes. Cell adhesion molecules play a critical role in the re-localization and survival of CLL cells. Understanding the molecular mechanisms underlying their activation and functions has led to the development of new therapeutic strategies. This review summarizes the current knowledge on the microenvironment, cell adhesion molecules, and their role in transendothelial migration and cell adhesion-mediated drug resistance in CLL. The review also discusses how novel therapeutic compounds modulate the functions of these molecules.
Article
Medicine, Research & Experimental
Marina Anastasiou, Gail A. Newton, Kuljeet Kaur, Francisco J. Carrillo-Salinas, Sasha A. Smolgovsky, Abraham L. Bayer, Vladimir Ilyukha, Shruti Sharma, Alexander Poltorak, Francis W. Luscinskas, Pilar Alcaide
Summary: Research has shown that the lack of STING in mice impairs T cell infiltration into peritoneum, while STING plays a crucial role in T cell transendothelial migration, dependent on CXCL10 and IFNI/IFNAR signaling.
Article
Medicine, Research & Experimental
Pilar Alcaide
Summary: T-cell interaction with endothelial cells plays a crucial role in the inflammatory response, allowing T cells to migrate from the circulation to sites of infection or sterile inflammation. Understanding the key players in these interactions may lead to the development of novel therapeutics that can modulate T-cell migration and inflammation.
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2022)
Article
Multidisciplinary Sciences
Guillaume Serwe, David Kachaner, Jessica Gagnon, Cedric Plutoni, Driss Lajoie, Eloise Durame, Malha Sahmi, Damien Garrido, Martin Lefrancois, Genevieve Arseneault, Marc K. Saba-El-Leil, Sylvain Meloche, Gregory Emery, Marc Therrien
Summary: Cell motility is a critical feature of invasive tumour cells that is governed by complex signal transduction events. Here, researchers have identified a novel pro-motility pathway in cancer cells, in which the scaffold protein CNK2 and its partner SAMD12 play a crucial role in promoting cell migration by activating ARF6 GTPase.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Janine J. G. Arts, Eike K. Mahlandt, Max L. B. Gronloh, Lilian Schimmel, Ivar Noordstra, Emma Gordon, Abraham C. van Steen, Simon Tol, Barbara Walzog, Jos van Rijssel, Martijn A. Nolte, Marten Postma, Satya Khuon, John M. Heddleston, Eric Wait, Teng Leong Chew, Mark Winter, Eloi Montanez, Joachim Goedhart, Jaap D. van Buul
Summary: During inflammation, leukocytes rapidly transmigrate across the endothelium by choosing membrane protrusions at cell junctions, which depend on Rac1 activity. They cross the junction by migrating underneath the protruding endothelial cell, facilitating their exit from inflamed vessel walls.
Article
Veterinary Sciences
L. Etchevers, M. S. Renna, E. M. Belotti, P. U. Diaz, N. R. Salvetti, H. H. Ortega, A. N. Amweg
Summary: ACTH treatment decreases the expression of adhesion molecules in PBMCs and endothelial cells, reducing the infiltration of PBMCs and alleviating the inflammatory response during ovulation in cows.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Oncology
Leila Mohammadi, Bashir Mosayyebi, Mahsa Imani, Mohammad Rahmati
Summary: Dexamethasone can affect the migration and adhesion of T47D breast cancer cells by regulating the expression levels of cell adhesion molecules and MMPs. It decreases the viability of cells and alters the expression of these molecules. These findings suggest that dexamethasone may play a role in preventing metastasis in breast cancer.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Giacomo S. Amelio, Livia Provitera, Genny Raffaeli, Ilaria Amodeo, Silvia Gulden, Valeria Cortesi, Francesca Manzoni, Nicola Pesenti, Matteo Tripodi, Valentina Pravata, Caterina Lonati, Gaia Cervellini, Fabio Mosca, Giacomo Cavallaro
Summary: This study investigates the perinatal endothelial phenotype in very preterm infants and its predictive value on neonatal mortality and hemodynamic and respiratory complications. The results suggest that very preterm infants have a proinflammatory endothelial phenotype, possibly resulting from a combination of pathological prenatal inheritance and premature extrauterine transition.
Article
Medicine, Research & Experimental
Cong Zhang, Zhuowen Yang, Peng Zhou, Muxin Yu, Baorong Li, Yingmiao Liu, Jiaqi Jin, Wenhui Liu, Haijiao Jing, Jingwen Du, Jie Tian, Zhiyu Zhao, Jianxin Wang, Yinzhu Chu, ChunMei Zhang, Valerie A. Novakovic, Jialan Shi, Changjun Wu
Summary: The study found that neoadjuvant chemotherapy significantly increased the expression of PS+ BCMPs in patient plasma, which was associated with a rapid increase in procoagulant activity. Treatment with lactadherin reduced the adhesion of BCMPs and their procoagulant activity. Injection of BCMPs in mice induced a hypercoagulable state, reducing fibrinogen levels and promoting thrombus formation.
Review
Biochemistry & Molecular Biology
Ghassan Bkaily, Danielle Jacques
Summary: The vascular endothelium is crucial in embryogenesis and aging, and it lines the blood vessels as a cell monolayer. It is recognized by the immune system as part of the body, so any abnormality in the endothelium exposes tissues to the immune system, leading to inflammation and vascular diseases like atherosclerosis. The endothelium's secretory role allows it to release vasoconstrictors, vasorelaxants, and cardio-modulatory factors that maintain proper circulatory system functioning. Adhesion molecules play a significant role in cardiovascular physiology and pathology by providing a seal for the monolayer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anu Autio, Huan Wang, Francisco Velazquez, Gail Newton, Charles A. Parkos, Pablo Engel, Daniel Engelbertsen, Andrew H. Lichtman, Francis W. Luscinskas
Summary: The SIRP alpha-CD47 axis plays an important role in T cell antigen priming. The reduced numbers and impaired migration of CD4(+) conventional dendritic cells (cDCs) in Cd47KO and SKI mice lead to reduced proximity between T cells and DCs in the splenic T cell zone, as well as decreased TCR activation, cell division, proliferation, and increased apoptosis in T cells.
Article
Cell Biology
Sofia K. H. Morsing, Timo Rademakers, Sanne L. N. Brouns, Anne-Marieke D. van Stalborch, Marjo M. P. C. Donners, Jaap D. van Buul
Summary: The study reveals that endothelial ADAM10 plays a role in facilitating neutrophil transendothelial migration by cleaving ICAM-1, supporting the efficient release of neutrophils from the endothelium. Silencing or inhibiting endothelial ADAM10 leads to a significantly longer diapedesis step for neutrophils due to impaired dissociation from ICAM-1. Increased ICAM-1 levels on transmigrating neutrophils and potential membrane transfer from endothelium to neutrophils are also observed.
Article
Peripheral Vascular Disease
Joana Amado-Azevedo, Anne-Marieke D. van Stalborch, Erik T. Valent, Kalim Nawaz, Jan van Bezu, Etto C. Eringa, Femke P. M. Hoevenaars, Iris M. De Cuyper, Peter L. Hordijk, Victor W. M. van Hinsbergh, Geerten P. van Nieuw Amerongen, Jurjan Aman, Coert Margadant
Summary: The kinase Arg/Abl2 has been identified as a mediator of endothelial barrier disruption. Depletion of Arg in endothelial cells resulted in activation of RhoA and Rac1, increased cell spreading and elongation, and redistribution of integrin-dependent cell-matrix adhesions. In vivo, genetic deletion of Arg diminished vascular leak in the skin and lungs, suggesting therapeutic inhibition of Arg may be a feasible strategy for treating conditions characterized by vascular leak.
Review
Cell Biology
Max L. B. Gronloh, Janine J. G. Arts, Jaap D. van Buul
Summary: Leukocyte transendothelial migration (TEM) during inflammation involves several established steps, such as rolling, adhesion, crawling, diapedesis, and sub-endothelial crawling. Factors like chemokine cues, adhesion molecules, membrane protrusions, and physical factors contribute to the formation of transmigration hotspots where leukocytes exit the vasculature.
JOURNAL OF CELL SCIENCE
(2021)
Article
Surgery
Natalija Bogunovic, Jorn P. Meekel, Jisca Majolee, Marije Hekhuis, Jakob Pyszkowski, Stefan Jockenhoevel, Magnus Kruse, Elise Riesebos, Dimitra Micha, Jan D. Blankensteijn, Peter L. Hordijk, Samaneh Ghazanfari, Kak K. Yeung
Summary: The study presented a novel 3D vascular model for investigating SMC spatial organization and SMC-ECM interactions in the context of AAA. Comparison between controls and patients revealed differences in cell morphology, orientation, mechanical properties, and ECM production, with implications for understanding AAA pathophysiology. This patient-specific model shows promise for translational research and therapeutic developments in the field.
JOURNAL OF ENDOVASCULAR THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Thijs J. Sluiter, Jaap D. van Buul, Stephan Huveneers, Paul H. A. Quax, Margreet R. de Vries
Summary: The vascular endothelium acts as a specialized barrier controlling the passage of fluids and cells, particularly leukocytes. Impaired barrier function in atherosclerosis leads to uncontrolled leukocyte extravasation and vascular leakage, contributing to plaque destabilization and intraplaque hemorrhage.
Correction
Peripheral Vascular Disease
Joana Amado-Azevedo, Anne-Marieke D. van Stalborch, Erik T. Valent, Kalim Nawaz, Jan van Bezu, Etto C. Eringa, Femke P. M. Hoevenaars, Iris M. De Cuyper, Peter L. Hordijk, Victor W. M. van Hinsbergh, Geerten P. van Nieuw Amerongen, Jurjan Aman, Coert Margadant
Article
Critical Care Medicine
Xue D. Manz, Robert Szulcek, Xiaoke Pan, Petr Symersky, Chris Dickhoff, Jisca Majolee, Veerle Kremer, Elisabetta Michielon, Ekaterina S. Jordanova, Teodora Radonic, Irene Bijnsdorp, Sander R. Piersma, Thang Pham, Connie R. Jimenez, Anton Vonk Noordegraaf, Frances S. de Man, Reinier A. Boon, Jan Voorberg, Peter L. Hordijk, Jurjan Aman, Harm Jan Bogaard
Summary: This study identifies the role of von Willebrand factor (vWF) in chronic thromboembolic pulmonary hypertension (CTEPH). Increased vWF levels in plasma and pulmonary endothelium of CTEPH patients contribute to elevated platelet adhesion to CTEPH endothelium. The increased expression of nuclear factor (NF)-kappa B2 and epigenetic modifications of the vWF promoter play a critical role in driving vWF transcription and promoting a prothrombotic phenotype in CTEPH-pulmonary artery endothelial cells.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart
Summary: The study introduces a new genetically encoded calcium indicator, Tq-Ca-FLITS, which enables reliable measurement of intracellular calcium concentrations through fluorescence lifetime imaging, addressing the quantification issues associated with existing GECIs.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Marieke Goedhart, Edith Slot, Maria F. Pascutti, Sulima Geerman, Timo Rademakers, Benjamin Nota, Stephan Huveneers, Jaap D. van Buul, Katherine C. MacNamara, Carlijn Voermans, Martijn A. Nolte
Summary: Apart from controlling hematopoiesis, the bone marrow also functions as a secondary lymphoid organ. Bone marrow-resident dendritic cells (DCs) are mainly localized around sinusoids and can present antigens. They belong to the cDC2 subtype and are associated with Th2/Th17 immunity. These DCs express several c-type lectins, including Dectin-1, which can bind fungal beta-glucans. Compared to spleen DCs, bone marrow DCs are more efficient in phagocytosing yeast particles and Aspergillus conidia, and this is dependent on Dectin-1. Additionally, zymosan-stimulated bone marrow DCs enhance neutrophil differentiation and maintenance of progenitor cells.
Article
Hematology
Sofia K. H. Morsing, Eveline Zeeuw van der Laan, Annemarieke D. van Stalborch, Jaap D. van Buul, Rick Kapur, Alexander P. Vlaar
Summary: The study found that the combination of LPS and HLA class I antibody can have a synergistic effect on pulmonary endothelial cells, leading to enhanced inflammatory responses.
Article
Biochemistry & Molecular Biology
Max L. B. Gronloh, Janine J. G. Arts, Sebastian Palacios Martinez, Amerens A. van Der Veen, Lanette Kempers, Abraham C. van Steen, Joris J. T. H. Roelofs, Martijn A. Nolte, Joachim Goedhart, Jaap D. van Buul
Summary: Using a photoconvertible probe, researchers traced and identified the original endothelial transmigration hotspots, and found that these hotspots function to limit vascular leakage during transmigration events. The distribution of ICAM-1 determines the location of the transmigration hotspot.
Article
Biochemistry & Molecular Biology
Fabienne Podieh, Roos Wensveen, Max C. Overboom, Lotte Abbas, Jisca Majolee, Peter L. Hordijk
Summary: Endothelial monolayer permeability is regulated by actin dynamics and vesicular traffic. Inhibition of E1 ubiquitin ligases leads to a rapid, reversible loss of integrity in quiescent endothelial monolayers with increased F-actin stress fibers and intercellular gaps. Fast protein turnover of negative regulators of cell-cell contact is essential for maintaining monolayer integrity in quiescent human endothelial cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biology
Eike K. Mahlandt, Sebastian Palacios Martinez, Janine J. G. Arts, Simon Tol, Jaap D. van Buul, Joachim Goedhart
Summary: This study investigates the role of Rho GTPases in the vascular barrier of endothelial cells using optogenetics. The results show that by precisely controlling the activation of Rho GTPases, the strength of the vascular barrier can be precisely regulated in a VE-cadherin-independent manner through cell-cell overlap-dependent mechanism. Furthermore, optogenetic control over morphological features of endothelial cells, such as cell size and shape, is also demonstrated.
Article
Medicine, General & Internal
Daan P. van den Brink, Derek J. B. Kleinveld, Annabel Bongers, Jael Vos, Joris T. H. Roelofs, Nina C. Weber, Jaap D. van Buul, Nicole P. Juffermans, Toshiaki Iba
Summary: In a rat pneumosepsis model, supplementing fluid resuscitation with heparan sulfate did not reduce organ or endothelial injury.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Max Laurens Bastiaan Gronloh, Janine Johanna Geertruida Arts, Eike Karin Mahlandt, Martijn A. Nolte, Joachim Goedhart, Jaap Diederik van Buul
Summary: During inflammation, primary neutrophil adhesion to the endothelium triggers the formation of transmigration hotspots, allowing secondary neutrophils to cross the endothelium more efficiently. This process is mediated by the activation of JNK1 and MARCKSL1, which induces the formation of filopodia and increases neutrophil adhesion.
