Article
Hematology
Genevieve Marcoux, Audree Laroche, Stephan Hasse, Marie Bellio, Maroua Mbarik, Marie Tamagne, Isabelle Allaeys, Anne Zufferey, Tania Levesque, Johan Rebetz, Annie Karakeussian-Rimbaud, Julie Turgeon, Sylvain G. Bourgoin, Hind Hamzeh-Cognasse, Fabrice Cognasse, Rick Kapur, John W. Semple, Marie-Josee Hebert, France Pirenne, Herman S. Overkleeft, Bogdan I. Florea, Melanie Dieude, Benoit Vingert, Eric Boilard
Summary: Platelets not only play a role in hemostasis but also have an important function in immunity by releasing extracellular vesicles (EVs) when activated. These platelet-derived EVs (PEVs) contain the active 20S proteasome, MHC-I, and lymphocyte costimulatory molecules. PEVs contribute to adaptive immunity by cross-presentation of antigens and have privileged access to immune cells through the lymphatic system.
Review
Cell Biology
Zhi Cai, Junyan Feng, Nian Dong, Pan Zhou, Yuanshuai Huang, Hongwei Zhang
Summary: Platelet-derived extracellular vesicles (PEVs) not only have procoagulant activity but also contain platelet-derived inflammatory factors that mediate inflammatory responses. Studies have shown that platelets are activated during storage to produce large amounts of PEVs, which may have implications for platelet transfusion therapy. Compared to platelets, PEVs have a longer storage time and greater procoagulant activity, making them an ideal alternative to platelets.
Article
Biochemistry & Molecular Biology
Yuki Kawai-Harada, Hanine El Itawi, Hiroaki Komuro, Masako Harada
Summary: Extracellular vesicles (EVs) serve as mediators of intercellular communication by transporting molecules between cells, and have potential clinical applications in diagnostics and therapeutics. However, standardized storage conditions for EVs are lacking, leading to challenges in clinical applications and potential misinterpretation of findings. This study aims to optimize the storage conditions of EVs and characterize them under different experimental conditions and models, reporting a highly efficient EV storage buffer that can protect their molecular cargo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jacob L. Leger, Marie-France N. Soucy, Vanessa Veilleux, Robert D. Foulem, Gilles A. Robichaud, Marc E. Surette, Eric P. Allain, Luc H. Boudreau
Summary: Inflammation is a crucial defense process against infections, illness, or tissue damage, and polymorphonuclear neutrophils (PMN) are among the first immune cells involved. Recent studies have shown that platelets (PLTs), in addition to their role in blood coagulation, also play a role in inflammatory responses by releasing functional mitochondria (freeMitos). This study reveals that freeMitos increase mitochondrial gene expression in PMN and significantly induce the release of microvesicles.
Review
Biochemistry & Molecular Biology
Miquel Antich-Rossello, Maria Antonia Forteza-Genestra, Marta Monjo, Joana M. Ramis
Summary: Platelet-derived EVs (pEVs) show great potential as a new therapeutic approach in wound healing and regenerative medicine. Studies have demonstrated that pEVs not only participate in healing responses but also induce cellular differentiation, promoting musculoskeletal or neural regeneration. However, there is heterogeneity in the obtention and characterization of pEVs, requiring further research to enhance their translation into feasible therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Meryem Mabrouk, Fadila Guessous, Abdallah Naya, Yahye Merhi, Younes Zaid
Summary: Platelet-derived extracellular vesicles play an important role in the blood, carrying diverse contents and performing various functions under different physiological and pathophysiological conditions.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Cardiac & Cardiovascular Systems
Tejasvi Dudiki, Manoj Veleeparambil, Irina Zhevlakova, Sudipta Biswas, Eric A. A. Klein, Peter Ford, Eugene A. A. Podrez, Tatiana V. V. Byzova
Summary: Thrombosis is a common complication in cancer patients and platelet hyperactivation plays a critical role. This study found that small extracellular vesicles (sEVs) released by cancer cells are efficiently taken up by platelets, leading to platelet activation and thrombosis. The discovery highlights the diagnostic and prognostic value of platelet-associated cancer markers and identifies new pathways for intervention.
CIRCULATION RESEARCH
(2023)
Review
Cell Biology
Jiuping Wu, Yingxin Piao, Qinyi Liu, Xiaoyu Yang
Summary: Platelet-rich plasma (PRP) has been used in regenerative medicine for over 30 years due to its promising therapeutic properties. Recent studies have found that extracellular vesicles (EVs) released from activated platelets also play a role in tissue repair, leading to increased research on PRP-derived EVs (PRP-EVs) for tissue regeneration as a superior cell-free therapeutic strategy.
CELL PROLIFERATION
(2021)
Correction
Cell & Tissue Engineering
Yifeng Ke, Xiaoe Fan, Rui Hao, Lijie Dong, Min Xue, Liangzhang Tan, Chunbo Yang, Xiaorong Li, Xinjun Ren
Summary: The amended version of this paper has been published and can be accessed through the original article.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Leslie Marisol Lugo-Gavidia, Janis M. Nolde, Revathy Carnagarin, Dylan Burger, Justine Chan, Sandi Robinson, Erika Bosio, Vance B. Matthews, Markus P. Schlaich
Summary: This study aimed to investigate the relationship between circulating platelet-derived extracellular vesicles (EVs) and cardiovascular risk stratification and vascular organ damage. The results showed that EV levels were associated with the severity of cardiovascular risk and macrovascular organ damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Rosa Suades, Teresa Padro, Gemma Vilahur, Lina Badimon
Summary: Platelets play essential roles in thrombosis, inflammation, and angiogenesis. Stimulation by thrombin triggers the release of pEVs from platelets, which are rich in procoagulant and proadhesive proteins. Differential proteomic analysis revealed significant changes in protein composition between pEVs from activated platelets and non-activated platelets.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Renato Simoes Gaspar, Plinio M. Ferreira, Joanne L. Mitchell, Giordano Pula, Jonathan M. Gibbins
Summary: The study shows that PDEVs contain Nox-1 and can induce platelet activation through Nox1-mediated superoxide generation. Inhibition of Nox-1 eliminates PDEV-mediated platelet activation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Immunology
Ehsan Gharib, Vanessa Veilleux, Luc H. Boudreau, Nicolas Pichaud, Gilles A. Robichaud
Summary: Inflammation and platelets promote cancer malignancy. Platelet-derived microparticles (PMPs) induce metabolic plasticity and disease progression in chronic lymphocytic leukemia (CLL). PMPs enhance CLL tumor growth and invasiveness through mitochondrial internalization and OXPHOS stimulation, as well as increase resistance to chemotherapy drugs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Haotian Luo, Danying Chen, Ruoyu Li, Runze Li, Yungshan Teng, Yang Cao, Xuenong Zou, Weicai Wang, Chen Zhou
Summary: Biofilm-related diseases are difficult to treat due to their tolerance to antimicrobial chemotherapies. Periodontitis, a chronic biofilm disease induced by dental plaque, provides a good model to study the effects of host factors on the biofilm microenvironment. This study confirmed the reduction of microRNA-126 (miR-126) with the recruitment of macrophages in periodontitis and explored a strategy for targeted delivery of miR-126 to macrophages. Exosomes overexpressing CXCR4 and loaded with miR-126 successfully regulated macrophages and reduced bone resorption and osteoclastogenesis in periodontitis. These findings provide new insights for the treatment of periodontitis and other biofilm-related diseases.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Cell Biology
Benshuai You, Chenglin Zhou, Yang Yang
Summary: Osteoarthritis is the main cause of disability in older adults and severely affects quality of life. Multiple factors are involved in its pathogenesis and existing therapies are relatively ineffective. However, mesenchymal stem cell-related therapies mediated by extracellular vesicles have shown positive outcomes for the treatment of osteoarthritis.
AGEING RESEARCH REVIEWS
(2023)
Article
Gastroenterology & Hepatology
Yuhan Yin, Anna Sichler, Josef Ecker, Melanie Laschinger, Gerhard Liebisch, Marcus Hoering, Marijana Basic, Andre Bleich, Xue-Jun Zhang, Ludwig Kuebelsbeck, Johannes Plagge, Emely Scherer, Dirk Wohlleber, Jianye Wang, Yang Wang, Marcella Steffani, Pavel Stupakov, Yasmin Gaertner, Fabian Lohoefer, Carolin Mogler, Helmut Friess, Daniel Hartmann, Bernhard Holzmann, Norbert Hueser, Klaus-Peter Janssen
Summary: Hepatocyte growth and proliferation depend on membrane phospholipid biosynthesis, which is significantly influenced by short-chain fatty acids (SCFAs) generated through bacterial fermentation. Antibiotic treatment and dysbiosis not only affect gut microbiota, but also impair hepatic lipid synthesis and liver regeneration.
JOURNAL OF HEPATOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Nikolaos Pagonas, Felix S. Seibert, Gerhard Liebisch, Maximillian Seidel, Theodoros Giannakopoulos, Benjamin Sasko, Oliver Ritter, Nina Babel, Timm H. Westhoff
Summary: This study found an association between low levels of propionate, a short-chain fatty acid, and coronary artery disease (CAD).
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Silvia Friedrichs, Dieter Luetjohann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: Replacing soybean oil with Hermetia illucen larvae fat alters the fatty acid composition of meat and reduces lipid oxidation.
ARCHIVES OF ANIMAL NUTRITION
(2023)
Article
Hematology
Lilli Arndt, Ileana Hernandez-Resendiz, Doreen Moos, Janine Dokas, Silvana Mueller, Franziska Jeromin, Richard Wagner, Uta Ceglarek, Iris M. Heid, Marcus Hoering, Gerhard Liebisch, Sonja C. Stadler, Ralph Burkhardt
Summary: This study demonstrates that deficiency of Trib1 promotes the formation of atherosclerotic lesions and increases plasma cholesterol and triglyceride levels in Ldlr(-/-) mice. It also suggests that Trib1 deficiency alters hepatic lipid metabolism and induces inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Genetics & Heredity
Lisa Rein-Fischboeck, Rebekka Pohl, Elisabeth M. Haberl, Wolfgang Mages, Philipp Girke, Gerhard Liebisch, Sabrina Krautbauer, Christa Buechler
Summary: Visceral adiposity is associated with liver steatosis and the development of NASH. Mice lacking SNTB2 have reduced intra-abdominal fat mass and do not accumulate hepatic cholesteryl esters on a MCD diet. However, they still develop a similar NASH pathology as wild type mice, suggesting a minor role of intra-abdominal fat and liver cholesteryl esters in this model.
Article
Biochemistry & Molecular Biology
Georg Peschel, Kilian Weigand, Jonathan Grimm, Martina Mueller, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Christa Buechler
Summary: This study analyzed the composition of sphingomyelin (SM) in the serum of patients with chronic hepatitis C virus (HCV) infection, and found differences in SM species among different genders, genotypes, and patients with liver cirrhosis. The levels of SM were more closely associated with liver function in male patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Coenraad Frederik Slabber, Marc Bachofner, Tobias Speicher, Andrii Kuklin, Abbie Elisabeth Fearon, Susagna Padrissa-Altes, Roman Bogorad, Carla Horvath Rudigier, Daria Wust, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Daniel G. Anderson, Christian Wolfrum, Ulrich Auf Dem Keller, Sabine Werner
Summary: Mice lacking FGF receptors 1 and 2 (Fgfr1 and Fgfr2) in hepatocytes are hypersensitive to cytotoxic injury during liver regeneration, which is associated with the protective role of the urinary-reproductive factor Uhrf2 in liver cells. Uhrf2 expression increases during regeneration after partial hepatectomy in an FGFR-dependent manner, but the abundance of Uhrf2 in liver cells is lower in FGFR-deficient mice. Hepatocyte-specific knockout of Uhrf2 leads to liver necrosis, impaired hepatocyte proliferation, and liver failure.
Article
Multidisciplinary Sciences
Susanne Heimerl, Marcus Hoering, Dominik Kopczynski, Alexander Sigruener, Christina Hart, Ralph Burkhardt, Anne Black, Robert Ahrends, Gerhard Liebisch
Summary: This study presents a standardized protocol for quantifying lipid species concentrations in basal and activated human platelets using flow-injection mass spectrometry. The results show changes in lipid species concentrations after platelet activation and the release of complex lipids. These concentrations provide important insights into the physiological role of platelet lipids and serve as a basis for studying the platelet lipidome in patients with hemorrhagic or thrombotic disorders.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Garima Maheshwari, Marcus Hoering, Gerhard Liebisch, Erika Most, Klaus Eder, Robert Ringseis
Summary: This study comprehensively investigated the effect of using insect fat from Hermetia illucens larvae as a fat source in broiler diets. The results showed that replacing soybean oil with insect fat had no effect on the growth performance and metabolic health of broilers, suggesting that insect fat can be a sustainable alternative fat source in broiler diets.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Fen Long, Memoona R. Bhatti, Alexandra Kellenberger, Wenfei Sun, Salvatore Modica, Marcus Hoering, Gerhard Liebisch, Jean-Philippe Krieger, Christian Wolfrum, Tenagne D. Challa
Summary: This study reveals that a high-fat diet can cause liver fibrosis and non-alcoholic fatty liver disease, and explores the mechanisms through which these diseases occur under strict diet control.
MOLECULAR METABOLISM
(2023)
Article
Chemistry, Analytical
Dominik Kopczynski, Nils Hoffmann, Nina Troppmair, Cristina Coman, Kim Ekroos, Michael R. Kreutz, Gerhard Liebisch, Dominik Schwudke, Robert Ahrends
Summary: Lipid analysis is important for understanding the various functions of lipids, and LipidSpace is a standalone tool that analyzes lipidomes by assessing their structural and quantitative differences. It offers a user-friendly GUI and support for multiple data formats, allowing for reanalysis and merging of datasets, and provides additional discoveries.
ANALYTICAL CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Sarah M. Grundmann, Kerstin Ress, Lea Zimmermann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: A high phosphorus intake is associated with metabolic disorders and this study investigated the impact of a high-phosphorus diet on mouse skeletal muscle lipid composition and gene transcription.
Article
Cell Biology
Thomas Timm, Christiane Hild, Gerhard Liebisch, Markus Rickert, Guenter Lochnit, Juergen Steinmeyer
Summary: The levels of lysophosphatidylcholine (LPC) are elevated in the synovial fluid of patients with osteoarthritis (OA). Different LPC species have varying effects on the regulation of proteins and phospholipids in fibroblast-like synoviocytes (FLSs). IL-1 & beta; has the most significant impact on the regulation of proteins by LPC 16:0, suggesting its potential role in modulating inflammatory and catabolic mechanisms.
Article
Biology
Sabrina Ehnert, Anna J. Schreiner, Claudine Seeliger, Josef Ecker, Fabian Springer, Gerhard Liebisch, Philipp Hemmann, Tina Histing, Andreas K. Nussler
Summary: Using fatty acid methyl ester gas chromatography-mass spectrometry, this study found that both saturated and unsaturated fatty acids increase in the bone marrow. Characteristic fatty acid patterns were identified in patients with normal BMD, osteopenia, and osteoporosis, and different patterns were found in plasma, red bone marrow, and yellow bone marrow. Specific fatty acids correlated with osteoclast activity and suggested a possible mechanism for how fatty acids interfere with BMD, but no single fatty acid in the profile could be claimed for controlling BMD.