Article
Biochemistry & Molecular Biology
Jeffrey M. Stolwijk, Jonathan A. Stefely, Mike T. Veling, Thomas J. van t Erve, Brett A. Wagner, Thomas J. Raife, Garry R. Buettner
Summary: The research revealed that GPx4 is an active enzyme in mature RBCs, protecting them from hemolysis during blood bank storage. It is correlated with lipid-anchored proteins and anti-correlated with lyso-phospholipids and complement system proteins.
Article
Surgery
Kasiemobi E. Pulliam, Bernadin Joseph, Amy T. Makley, Charles C. Caldwell, Alex B. Lentsch, Michael D. Goodman, Timothy A. Pritts
Summary: The study showed that washing packed red blood cells during storage can decrease the development of red blood cell storage lesions, reducing microvesicle accumulation and cell-free hemoglobin, with no impact on red blood cell viability.
Review
Physiology
Antreas C. Kalli, Reinhart A. F. Reithmeier
Summary: Molecular dynamics simulations provide valuable insights into the structure and function of the red blood cell Band 3 anion exchanger. These simulations reveal specific lipid and protein interactions and have been used to reconstruct a model of the Wright blood group antigen complex of Band 3 and Glycophorin A. Current research is focused on describing the real-time trajectory of substrate binding and translocation.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Physiology
Gregory Barshtein, Ivana Pajic-Lijakovic, Alexander Gural
Summary: Red blood cells' deformability plays a crucial role in adapting to flow conditions, with high deformability facilitating flow through small blood vessels. Loss of deformability may lead to cell rigidity and dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gregory Barshtein, Alexander Gural, Dan Arbell, Refael Barkan, Leonid Livshits, Ivana Pajic-Lijakovic, Saul Yedgar
Summary: Reduced deformability of red blood cells (RBCs) is associated with various circulatory disorders and anemias, and is linked to metabolic and structural alterations. This study identified 14 proteins that are positively correlated with RBC deformability, suggesting that the level of one or a few of these proteins can be used to assess cell deformability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Myrthe J. van Dijk, Brigitte A. van Oirschot, Alexander N. Harrison, Steffen M. Recktenwald, Min Qiao, Amaury Stommen, Anne-Sophie Cloos, Juliette Vanderroost, Romano Terrasi, Kuntal Dey, Jennifer Bos, Minke A. E. Rab, Anna Bogdanova, Giampaolo Minetti, Giulio G. Muccioli, Donatienne Tyteca, Stephane Egee, Lars Kaestner, Robert S. Molday, Eduard J. van Beers, Richard van Wijk
Summary: Mutations in the ATP11C gene result in decreased activity of the PS flippase, leading to hemolytic anemia. The deficiency of ATP11C causes an increase in PS exposure on the red blood cell membrane, abnormal membrane structure, and altered flow behavior of red blood cells.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Biochemical Research Methods
Rui Liu, Meng Shao, Zeyu Ke, Changxu Li, Fengya Lu, Min-cheng Zhong, Yuxin Mao, Xunbin Wei, Zhensheng Zhong, Jinhua Zhou
Summary: Measuring the deformability of RBCs is crucial for transfusion and gas transport ability of RBCs during storage. However, traditional methods have low efficiency in measuring the deformability of RBCs with different morphologies. This study developed a cost-effective device and successfully measured the deformability of RBCs with different morphologies.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Food Science & Technology
Naiyan Lu, Jiyue Wang, Zhe Chen, Xuan Zhang, Chen Chen, Sisi Wang
Summary: The study found that pretreating milk samples with added phospholipids resulted in smaller MFGs particle size, lower ζ-potential, and improved storage stability. Laser confocal microscopy revealed that more surface area of MFGs was covered by phospholipids. Additionally, SDS-PAGE patterns showed that adding phospholipids reduced serum protein absorption and preserved more native MFGM proteins.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biophysics
Bing -De Zheng, Mei-tian Xiao
Summary: Nano-RBMs is a disguised therapeutic platform that can resist recognition by the immune system and prolong the circulation time of the treatment agent in the blood, achieving ideal phototherapy effects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Multidisciplinary Sciences
Lu Han, Lianlian Li, Hangya Linghu, Lei Zheng, Daming Gou
Summary: This study investigated whether transfusion affects the lifespan of red blood cells. The results showed that bypass plasma altered the morphology of red blood cells, increased oxidative stress, caspase-3 activity, and phosphatidylserine exposure, while decreasing ATP level. Pre-treating red blood cells attenuated the effects of bypass on caspase-3 activity.
Article
Biochemistry & Molecular Biology
Alkmini T. Anastasiadi, Vassilis L. Tzounakas, Vasiliki-Zoi Arvaniti, Monika Dzieciatkowska, Konstantinos Stamoulis, Marilena E. Lekka, Issidora S. Papassideri, Angelo D'Alessandro, Anastasios G. Kriebardis, Marianna H. Antonelou
Summary: Proteasome activity in stored red blood cells is influenced by storage time and donor characteristics, with higher activity observed in cells from beta-thalassemia trait donors. During storage, proteasomes show a close connection with oxidative stress and interact with chaperones and calpains. The interactomes of beta-thalassemia trait donors are enriched in kinases, metabolic enzymes, and proteins differentially expressed in their membranes.
Review
Biochemistry & Molecular Biology
Sandrine Laurance, Mickael Marin, Yves Colin
Summary: Central retinal vein occlusion (CRVO) is a common retinal disorder leading to blindness, but the primary cause and treatment remain unidentified. This review aims to provide an overview of CRVO pathophysiology, specifically focusing on the role of red blood cells (RBCs). The review explores the functions of RBCs beyond oxygen transport and their potential involvement in CRVO occurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Juan Cata, Maria Ramirez, Patrice Forget, Lee-Lynn Chen, Oscar Diaz-Cambronero, Wankun Chen, Matthew A. Warner, Adriana Knopfelmacher Couchonal, Paolo Pelosi, Luis Cuellar, German Corrales, Carlos Romero, Francisco Lobo, Leif Saager, Jorge Castro Tapia, Roy Kiberenge, Lei Feng, Ary Serpa Neto
Summary: The ARCA-1 study aims to assess the incidence and associations of perioperative blood transfusions with 1-year survival in a large cohort of patients undergoing major cancer surgery. The study is a prospective international multicentre observational study approved by the Institutional Review Board and will be disseminated at international conferences.
Article
Physiology
Giacomo Marchi, Claudia Bozzini, Lorenzo Bertolone, Francesco Dima, Fabiana Busti, Annalisa Castagna, Chiara Stranieri, Anna Maria Fratta Pasini, Simonetta Friso, Giuseppe Lippi, Domenico Girelli, Alice Vianello
Summary: Peripheral blood smear is still a valuable tool for diagnosing blood cell abnormalities despite advances in automated and molecular techniques. The morphological abnormalities of red blood cells in COVID-19 patients may be associated with immune-inflammatory stress hematopoiesis and have a certain correlation with mortality rate.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
Teresa Tiffert, Virgilio L. Lew
Summary: Studies have shown that hypertonic transitions can cause irreversible osmotic collapse of sealed membrane vesicles, which is critical for the proper interpretation of experimental results during functional studies.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Osha Roopnarine, David D. Thomas
Summary: The study found that ten actin-binding compounds affect the interaction between cardiac myosin subfragment 1 (S1) and pyrene-labeled F-actin (PFA), increasing ATP affinity for actin-bound myosin and slowing the transition of actin-myosin interaction in the early ATPase cycle. This work elucidates the mechanisms of action for these compounds, providing valuable insights for potential treatments of hypercontractile forms of cardiomyopathy and future drug discovery efforts for heart failure.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ang Li, Samantha L. Yuen, Daniel R. Stroik, Evan Kleinboehl, Razvan L. Cornea, David D. Thomas
Summary: The study demonstrates that DWORF enhances SERCA2a function by competing with PLB for binding, potentially offering a novel therapeutic approach for heart failure.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Abir Majumdar, David J. Burban, Joseph M. Muretta, Andrew R. Thompson, Tiffany A. Engel, Damien M. Rasmussen, Manu V. Subrahmanian, Gianluigi Veglia, David D. Thomas, Nicholas M. Levinson
Summary: CDKs are master regulators of the eukaryotic cell cycle, requiring regulatory phosphorylation and cyclin binding for activation. The study explores the activation process of Cdk2 and its allosteric coupling with different regulators, revealing differences between Cdk2 and Cdk4 in allosteric wiring and substrate specificity.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biology
Cristina Olivieri, Caitlin Walker, Adak Karamafrooz, Yingjie Wang, V. S. Manu, Fernando Porcelli, Donald K. Blumenthal, David D. Thomas, David A. Bernlohr, Simon M. Sandford, Susan S. Taylor, Gianluigi Veglia
Summary: The fusion of the dynamic J-domain to PKA-C disrupts the internal allosteric network, causing attenuation of nucleotide/PKI binding cooperativity. This reduced allosteric cooperativity alters specific recognitions and interactions between substrates and regulatory partners, contributing to dysregulation exhibited by PKA-C-DNAJB1.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biology
Daniel K. Weber, U. Venkateswara Reddy, Songlin Wang, Erik K. Larsen, Tata Gopinath, Martin B. Gustavsson, Razvan L. Cornea, David D. Thomas, Alfonso De Simone, Gianluigi Veglia
Summary: Phospholamban (PLN) regulates cardiac Ca2+ transport response by phosphorylation or increased Ca2+ concentration, disrupting inhibitory contacts on the SERCA binding interface and enhancing Ca2+ transport. This study sheds light on the signal transduction mechanism operated by posttranslationally modified bitopic membrane proteins, addressing longstanding questions about SERCA regulation.
Correction
Biology
Cristina Olivieri, Caitlin Walker, Adak Karamafrooz, Yingjie Wang, V. S. Manu, Fernando Porcelli, Donald K. Blumenthal, David D. Thomas, David A. Bernlohr, Sanford M. Simon, Susan S. Taylor, Gianluigi Veglia
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thomas A. Bunch, Piyali Guhathakurta, Victoria C. Lepak, Andrew R. Thompson, Rhye-Samuel Kanassatega, Anna Wilson, David D. Thomas, Brett A. Colson
Summary: This study identified small-molecule inhibitors that affect the binding between cMyBP-C and actin, providing potential targets for heart failure drugs that mimic phosphorylation or disrupt interactions with actin/myosin. The fluorescence lifetime assay allowed for the detection of pharmacologically active compounds that interfere with cMyBP-C-actin binding. This validated high-throughput screen focused on cMyBP-C, a regulator of cardiac muscle contractility and a key factor in heart failure, for the first time.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sami Chu, Joseph M. Muretta, David D. Thomas
Summary: The study aimed to test whether the IHM structure is the structural basis for the SRX state, and the results indicated that the IHM structure is sufficient but not necessary to produce the SRX kinetic state.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mark D. Rustad, Osha Roopnarine, Razvan L. Cornea, David D. Thomas
Summary: Insufficient activity of SERCA, the integral transmembrane Ca2+ pump, contributes significantly to heart failure, a leading cause of death worldwide. The novel DWORF micropeptide has been identified as a muscle-specific SERCA effector that can reverse the inhibitory effects of PLB and activate SERCA independently. However, the structural basis for these functions has not yet been determined.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Philip A. A. Bidwell, Samantha L. L. Yuen, Ji Li, Kaja Berg, Robyn T. T. Rebbeck, Courtney C. C. Aldrich, Osha Roopnarine, Razvan L. L. Cornea, David D. D. Thomas
Summary: The sarco/endoplasmic reticulum Ca-ATPase (SERCA) is a critical ion pump for muscle function, and increasing its activity could alleviate muscle dysfunction. Through high-throughput screening, several compounds were discovered that can activate SERCA and improve calcium uptake in both cardiac and skeletal muscles.
Article
Biochemistry & Molecular Biology
Bengt Svensson, Florentin R. Nitu, Robyn T. Rebbeck, Lindsey M. Mcgurran, Tetsuro Oda, David D. Thomas, Donald M. Bers, Razvan L. Cornea
Summary: Current studies have shown that Ca2+ leakage from cardiomyocyte sarcoplasmic reticulum through hyperactive resting cardiac ryanodine receptor channels (RyR2) has a pro-arrhythmic effect. An exogenous peptide (DPc10) binding promotes leaky RyR2 in cardiomyocytes and reports on the endogenous state. Conversely, binding of calmodulin (CaM) inhibits RyR2 leak and low CaM affinity is indicative of leaky RyR2. These findings have led to the development of a FRET biosensor for drug discovery targeting RyR2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Elisa Bovo, Robyn T. Rebbeck, Osha Roopnarine, David D. Thomas, Razvan L. Cornea, Aleksey Zima
Summary: This study reveals that Compound 6 exhibits promising characteristics that can improve intracellular Ca2+ dynamics by selectively enhancing SERCA2a Ca2+ uptake.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Joseph M. Autry, Bengt Svensson, Samuel F. Carlson, Zhenhui Chen, Razvan L. Cornea, David D. Thomas, Stephanie J. Valberg
Summary: The enzymatic activity of SERCA from horse muscle was analyzed, and it was found that horse SR vesicles have a higher Ca2+ transport rate and increased luminal Ca2+ stores compared to rabbit SR vesicles. This may be due to a reduced SLN inhibition of SERCA and abundant expression of CASQ in horse myofibers.
VETERINARY SCIENCES
(2021)
Article
Biochemical Research Methods
Adak Karamafrooz, Jack Brennan, David D. Thomas, Laurie L. Parker
Summary: The DNAJB1-PRKACA fusion is a signature genetic event of FL-HCC, potentially upregulating oncogenic pathways through redirecting substrate recognition. By integrating cell-based and in vitro phosphoproteomics, substrates directly targeted by PKA and J-PKA(ca) were identified, with inhibitors showing differential effects on substrates in the presence of the fusion protein.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Robyn T. Rebbeck, Anna K. Andrick, Sarah A. Denha, Bengt Svensson, Piyali Guhathakurta, David D. Thomas, Thomas S. Hays, Adam W. Avery
Summary: This study established a high-throughput screening method for discovering potential disrupters of the interaction between mutant beta-III-spectrin ABD and actin in live cells, showing its effectiveness.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)